The Experts below are selected from a list of 33504 Experts worldwide ranked by ideXlab platform
Maureen R Hanson - One of the best experts on this subject based on the ideXlab platform.
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In vivoanalysis of interactions between GFP-labeled Microfilaments and plastid stromules
BMC Plant Biology, 2004Co-Authors: Ernest Y Kwok, Maureen R HansonAbstract:Background Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with Microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled Microfilaments and plastids. Results We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe Microfilaments and their relationship to stromules in vivo . Microfilaments were observed in close contact with stromules and plastid bodies of hypocotyl epidermis. Time-lapse confocal microscopy revealed that Microfilament rearrangements were associated with changes in plastid and stromule morphology and position. We also observed close interactions between mitochondria and stromules in double-labeled cells. Conclusion Our results indicate a correlation between the rearrangement of Microfilaments and changes in the shape and position of plastids and stromules. Stromules interact with Microfilaments that may also be utilized by mitochondria and other organelles. The interaction of Microfilaments and plastids is likely to be mediated by actin-binding proteins on the plastid envelope membrane.
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In vivo analysis of interactions between GFP-labeled Microfilaments and plastid stromules.
BMC plant biology, 2004Co-Authors: Ernest Y Kwok, Maureen R HansonAbstract:Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with Microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled Microfilaments and plastids. We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe Microfilaments and their relationship to stromules in vivo. Microfilaments were observed in close contact with stromules and plastid bodies of hypocotyl epidermis. Time-lapse confocal microscopy revealed that Microfilament rearrangements were associated with changes in plastid and stromule morphology and position. We also observed close interactions between mitochondria and stromules in double-labeled cells. Our results indicate a correlation between the rearrangement of Microfilaments and changes in the shape and position of plastids and stromules. Stromules interact with Microfilaments that may also be utilized by mitochondria and other organelles. The interaction of Microfilaments and plastids is likely to be mediated by actin-binding proteins on the plastid envelope membrane.
Ronald L Moore - One of the best experts on this subject based on the ideXlab platform.
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A Microfilament-ERUPTION MECHANISM FOR SOLAR SPICULES
The Astrophysical Journal, 2016Co-Authors: Alphonse C. Sterling, Ronald L MooreAbstract:Recent investigations indicate that solar coronal jets result from eruptions of small-scale chromospheric filaments, called minifilaments; that is, the jets are produced by scaled-down versions of typical-sized filament eruptions. We consider whether solar spicules might in turn be scaled-down versions of coronal jets, being driven by eruptions of "Microfilaments." Assuming a Microfilament's size is about a spicule's width ($\sim$300~km), the estimated occurrence number plotted against the estimated size of erupting filaments, minifilaments, and Microfilaments approximately follows a power-law distribution (based on counts of CMEs, coronal jets, and spicules), suggesting that many or most spicules could result from Microfilament eruptions. Observed spicule-base Ca II brightenings plausibly result from such Microfilament eruptions. By analogy with coronal jets, Microfilament eruptions might produce spicules with many of their observed characteristics, including smooth rise profiles, twisting motions, and EUV counterparts. The postulated Microfilament eruptions are presumably eruptions of twisted-core micro magnetic bipoles that are $\sim$1$".0$ wide. These explosive bipoles might be built and destabilized by merging and cancelation of magnetic-flux elements of $\sim$few$\times 100$~G and of size
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a Microfilament eruption mechanism for solar spicules
The Astrophysical Journal, 2016Co-Authors: Alphonse C. Sterling, Ronald L MooreAbstract:Recent investigations indicate that solar coronal jets result from eruptions of small-scale chromospheric filaments, called minifilaments; that is, the jets are produced by scaled-down versions of typical-sized filament eruptions. We consider whether solar spicules might in turn be scaled-down versions of coronal jets, being driven by eruptions of "Microfilaments." Assuming a Microfilament's size is about a spicule's width ($\sim$300~km), the estimated occurrence number plotted against the estimated size of erupting filaments, minifilaments, and Microfilaments approximately follows a power-law distribution (based on counts of CMEs, coronal jets, and spicules), suggesting that many or most spicules could result from Microfilament eruptions. Observed spicule-base Ca II brightenings plausibly result from such Microfilament eruptions. By analogy with coronal jets, Microfilament eruptions might produce spicules with many of their observed characteristics, including smooth rise profiles, twisting motions, and EUV counterparts. The postulated Microfilament eruptions are presumably eruptions of twisted-core micro magnetic bipoles that are $\sim$1$".0$ wide. These explosive bipoles might be built and destabilized by merging and cancelation of magnetic-flux elements of $\sim$few$\times 100$~G and of size <~$0".5$---$1".0$. If however spicules are relatively more numerous than indicated by our extrapolated distribution, then only a fraction of spicules might result from this proposed mechanism.
Ernest Y Kwok - One of the best experts on this subject based on the ideXlab platform.
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In vivoanalysis of interactions between GFP-labeled Microfilaments and plastid stromules
BMC Plant Biology, 2004Co-Authors: Ernest Y Kwok, Maureen R HansonAbstract:Background Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with Microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled Microfilaments and plastids. Results We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe Microfilaments and their relationship to stromules in vivo . Microfilaments were observed in close contact with stromules and plastid bodies of hypocotyl epidermis. Time-lapse confocal microscopy revealed that Microfilament rearrangements were associated with changes in plastid and stromule morphology and position. We also observed close interactions between mitochondria and stromules in double-labeled cells. Conclusion Our results indicate a correlation between the rearrangement of Microfilaments and changes in the shape and position of plastids and stromules. Stromules interact with Microfilaments that may also be utilized by mitochondria and other organelles. The interaction of Microfilaments and plastids is likely to be mediated by actin-binding proteins on the plastid envelope membrane.
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In vivo analysis of interactions between GFP-labeled Microfilaments and plastid stromules.
BMC plant biology, 2004Co-Authors: Ernest Y Kwok, Maureen R HansonAbstract:Plastid stromules are stroma-filled tubules that extend from the surface of plastids in higher plants and allow the exchange of protein molecules between plastids. These structures are highly dynamic; stromules change both their shape and position in the cytoplasm very rapidly. Previous studies with Microfilament inhibitors indicated that stromule shape and movement are dependent on the actin cytoskeleton. To learn more about the nature of the interactions of stromules and the cytoskeleton, we imaged fluorescently-labeled Microfilaments and plastids. We have used Arabidopsis thaliana plants expressing green fluorescent protein fused to the human actin-binding protein talin to observe Microfilaments and their relationship to stromules in vivo. Microfilaments were observed in close contact with stromules and plastid bodies of hypocotyl epidermis. Time-lapse confocal microscopy revealed that Microfilament rearrangements were associated with changes in plastid and stromule morphology and position. We also observed close interactions between mitochondria and stromules in double-labeled cells. Our results indicate a correlation between the rearrangement of Microfilaments and changes in the shape and position of plastids and stromules. Stromules interact with Microfilaments that may also be utilized by mitochondria and other organelles. The interaction of Microfilaments and plastids is likely to be mediated by actin-binding proteins on the plastid envelope membrane.
Alphonse C. Sterling - One of the best experts on this subject based on the ideXlab platform.
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A Microfilament-ERUPTION MECHANISM FOR SOLAR SPICULES
The Astrophysical Journal, 2016Co-Authors: Alphonse C. Sterling, Ronald L MooreAbstract:Recent investigations indicate that solar coronal jets result from eruptions of small-scale chromospheric filaments, called minifilaments; that is, the jets are produced by scaled-down versions of typical-sized filament eruptions. We consider whether solar spicules might in turn be scaled-down versions of coronal jets, being driven by eruptions of "Microfilaments." Assuming a Microfilament's size is about a spicule's width ($\sim$300~km), the estimated occurrence number plotted against the estimated size of erupting filaments, minifilaments, and Microfilaments approximately follows a power-law distribution (based on counts of CMEs, coronal jets, and spicules), suggesting that many or most spicules could result from Microfilament eruptions. Observed spicule-base Ca II brightenings plausibly result from such Microfilament eruptions. By analogy with coronal jets, Microfilament eruptions might produce spicules with many of their observed characteristics, including smooth rise profiles, twisting motions, and EUV counterparts. The postulated Microfilament eruptions are presumably eruptions of twisted-core micro magnetic bipoles that are $\sim$1$".0$ wide. These explosive bipoles might be built and destabilized by merging and cancelation of magnetic-flux elements of $\sim$few$\times 100$~G and of size
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a Microfilament eruption mechanism for solar spicules
The Astrophysical Journal, 2016Co-Authors: Alphonse C. Sterling, Ronald L MooreAbstract:Recent investigations indicate that solar coronal jets result from eruptions of small-scale chromospheric filaments, called minifilaments; that is, the jets are produced by scaled-down versions of typical-sized filament eruptions. We consider whether solar spicules might in turn be scaled-down versions of coronal jets, being driven by eruptions of "Microfilaments." Assuming a Microfilament's size is about a spicule's width ($\sim$300~km), the estimated occurrence number plotted against the estimated size of erupting filaments, minifilaments, and Microfilaments approximately follows a power-law distribution (based on counts of CMEs, coronal jets, and spicules), suggesting that many or most spicules could result from Microfilament eruptions. Observed spicule-base Ca II brightenings plausibly result from such Microfilament eruptions. By analogy with coronal jets, Microfilament eruptions might produce spicules with many of their observed characteristics, including smooth rise profiles, twisting motions, and EUV counterparts. The postulated Microfilament eruptions are presumably eruptions of twisted-core micro magnetic bipoles that are $\sim$1$".0$ wide. These explosive bipoles might be built and destabilized by merging and cancelation of magnetic-flux elements of $\sim$few$\times 100$~G and of size <~$0".5$---$1".0$. If however spicules are relatively more numerous than indicated by our extrapolated distribution, then only a fraction of spicules might result from this proposed mechanism.
Shigetou Namba - One of the best experts on this subject based on the ideXlab platform.
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Interaction between the membrane protein of a pathogen and insect Microfilament complex determines insect-vector specificity
Proceedings of the National Academy of Sciences of the United States of America, 2006Co-Authors: Shiho Suzuki, Ryo Arashida, Shigeyuki Kakizawa, Yasuyuki Yamaji, Hisashi Nishigawa, Kenro Oshima, Masashi Ugaki, Hee-young Jung, Shigetou NambaAbstract:Many insect-transmissible pathogens are transmitted by specific insect species and not by others, even if they are closely related. The molecular mechanisms underlying such strict pathogen–insect specificity are poorly understood. Candidatus Phytoplasma asteris, OY strain, line W (OY), is a phytopathogenic bacterium transmitted from plant to plant by sap-feeding insect vectors (leafhoppers). Our study focused on an abundant cell-surface membrane protein of the phytoplasma named antigenic membrane protein (Amp), which is not homologous with any reported functional protein. Immunofluorescence microscopy of the phytoplasma-infected insect showed that OY phytoplasma was localized to the Microfilaments of the visceral smooth muscle surrounding the insect’s intestinal tract. The affinity column assay showed that Amp forms a complex with three insect proteins: actin, myosin heavy chain, and myosin light chain. Amp–Microfilament complexes were detected in all OY-transmitting leafhopper species, but not in the non-OY-transmitting leafhoppers, suggesting that the formation of the Amp–Microfilament complex is correlated with the phytoplasma-transmitting capability of leafhoppers. Although several studies have reported interactions between pathogens and mammalian Microfilaments, this is an example of host-specific interactions between a bacterial surface protein and a host Microfilament in insect cells. Our data also suggest that the utilization of a host Microfilament may be a universal system for pathogenic bacteria infecting mammals or insects.