Microflora

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Hans Henrik Huss - One of the best experts on this subject based on the ideXlab platform.

  • comparison of the Microflora isolated from spoiled cold smoked salmon from three smokehouses
    Food Research International, 1998
    Co-Authors: Lisbeth Truelstrup Hansen, Hans Henrik Huss
    Abstract:

    Abstract The Microflora on spoiled, sliced and vacuum packed, cold-smoked salmon from three smokehouses was quantified and characterized in two independent experiments. Large variations in the Microflora were observed both within (i.e. among vacuum packs from the same batch) and among the smokehouses. Lactic acid bacteria dominated the Microflora, which reached 107 cfu g−1. Total viable counts of microorganisms alone were not related to quality, though spoilage characteristics were typical for microbiological spoilage. Among the lactic acid bacteria, Lactobacillus curvatus (ca 52–55%) was the most common species in both experiments with Lactobacillus sake, Lactobacillus plantarum, Carnobacterium spp. and Leuconostoc spp. present in smaller numbers. In some cases, large numbers of Enterobacteriaceae were also present and identified species were Serratia liquefaciens, Enterobacter agglomerans and Hafnia alvei. The Microflora on cold-smoked salmon appeared to be related to the source of contamination i.e. the raw material and/or the smokehouse rather than being specific for the product, thus rendering the identification of the specific spoilage organisms difficult.

  • microbiological quality and shelf life of cold smoked salmon from three different processing plants
    Food Microbiology, 1998
    Co-Authors: Lisbeth Truelstrup Hansen, Susanne Drewes Rontved, Hans Henrik Huss
    Abstract:

    Abstract Whole fillets and slices of cold-smoked salmon from the same batch were vacuum packed and stored at 5°C for up to 50 days, sampled regularly and analysed for sensory and microbiological changes. The experiment was performed twice with salmon from three different smokehouses. The taste panel rejected sliced salmon after 21–36 days and fillets after 32–49 days of storage with no apparent relation to the Microflora or salt content (4.1–6.1% in the water phase). Whole fillets developed the same spoilage characteristics, which included sweet, sour, bitter, faecal, ammonia and cabbage-like off-flavours, as sliced salmon but had a softer texture at the time of rejection. Plate count and Malthus detection times revealed that the Microflora varied in numbers and composition among the three smokehouses. The Microflora differed between fillets and sliced salmon, indicating the impact of the in-house flora on the size and composition of the Microflora. Lactic acid bacteria, Enterobacteriaceae , psychrotrophic marine vibrio and Photobacterium spp. dominated the Microflora of spoiled salmon. Use of conductometric measurements (Malthus) calibrated to traditional agar methods was not a suitable rapid microbiological method for determining the size and composition of the Microflora, because of the variable Microflora.

Chao Hui Cheng - One of the best experts on this subject based on the ideXlab platform.

  • fermentative hydrogen production from xylose using anaerobic mixed Microflora
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Chao Hui Cheng
    Abstract:

    Abstract Sewage sludge Microflora were anaerobically cultivated in a chemostat-type anaerobic bioreactor at a temperature of 35 ± 1 ∘ C , pH of 7.1 and hydraulic retention time of 12 h to determine the hydrogen production efficiency from xylose (20 g-COD/L). This enriched Microflora was used as a seed in batch experiments to investigate the pH and substrate concentration effects on hydrogen-producing bioactivity. It is demonstrated that the enriched mesophilic sewage sludge Microflora with a continuous feeding can produce hydrogen from xylose with hydrogen content of 32% (v/v) in the biogas. Each mole of xylose yields 0.7 moles of hydrogen and each gram of biomass produces 0.038 moles of hydrogen per day. According to the batch test results, changes in pH and xylose concentration could enhance the Microflora hydrogen production activity. Batch cultivation of this mixed Microflora at pH values of 6–7 and xylose concentrations of 20 g-COD/L resulted in high hydrogen production with a yield of 1.92–2.25 mol- H 2 /mol-xylose. This value is comparable to that from an enrichment culture. Strategies based on pH and xylose concentration controls for optimal hydrogen production from xylose using sewage sludge Microflora are proposed.

  • Fermentative hydrogen production from xylose using anaerobic mixed Microflora
    International Journal of Hydrogen Energy, 2006
    Co-Authors: Chiu-yue Lin, Chao Hui Cheng
    Abstract:

    Sewage sludge Microflora were anaerobically cultivated in a chemostat-type anaerobic bioreactor at a temperature of 35 ± 1 {ring operator} C, pH of 7.1 and hydraulic retention time of 12 h to determine the hydrogen production efficiency from xylose (20 g-COD/L). This enriched Microflora was used as a seed in batch experiments to investigate the pH and substrate concentration effects on hydrogen-producing bioactivity. It is demonstrated that the enriched mesophilic sewage sludge Microflora with a continuous feeding can produce hydrogen from xylose with hydrogen content of 32% (v/v) in the biogas. Each mole of xylose yields 0.7 moles of hydrogen and each gram of biomass produces 0.038 moles of hydrogen per day. According to the batch test results, changes in pH and xylose concentration could enhance the Microflora hydrogen production activity. Batch cultivation of this mixed Microflora at pH values of 6-7 and xylose concentrations of 20 g-COD/L resulted in high hydrogen production with a yield of 1.92-2.25 mol- H2/mol-xylose. This value is comparable to that from an enrichment culture. Strategies based on pH and xylose concentration controls for optimal hydrogen production from xylose using sewage sludge Microflora are proposed. © 2005 International Association for Hydrogen Energy.

Lisbeth Truelstrup Hansen - One of the best experts on this subject based on the ideXlab platform.

  • comparison of the Microflora isolated from spoiled cold smoked salmon from three smokehouses
    Food Research International, 1998
    Co-Authors: Lisbeth Truelstrup Hansen, Hans Henrik Huss
    Abstract:

    Abstract The Microflora on spoiled, sliced and vacuum packed, cold-smoked salmon from three smokehouses was quantified and characterized in two independent experiments. Large variations in the Microflora were observed both within (i.e. among vacuum packs from the same batch) and among the smokehouses. Lactic acid bacteria dominated the Microflora, which reached 107 cfu g−1. Total viable counts of microorganisms alone were not related to quality, though spoilage characteristics were typical for microbiological spoilage. Among the lactic acid bacteria, Lactobacillus curvatus (ca 52–55%) was the most common species in both experiments with Lactobacillus sake, Lactobacillus plantarum, Carnobacterium spp. and Leuconostoc spp. present in smaller numbers. In some cases, large numbers of Enterobacteriaceae were also present and identified species were Serratia liquefaciens, Enterobacter agglomerans and Hafnia alvei. The Microflora on cold-smoked salmon appeared to be related to the source of contamination i.e. the raw material and/or the smokehouse rather than being specific for the product, thus rendering the identification of the specific spoilage organisms difficult.

  • microbiological quality and shelf life of cold smoked salmon from three different processing plants
    Food Microbiology, 1998
    Co-Authors: Lisbeth Truelstrup Hansen, Susanne Drewes Rontved, Hans Henrik Huss
    Abstract:

    Abstract Whole fillets and slices of cold-smoked salmon from the same batch were vacuum packed and stored at 5°C for up to 50 days, sampled regularly and analysed for sensory and microbiological changes. The experiment was performed twice with salmon from three different smokehouses. The taste panel rejected sliced salmon after 21–36 days and fillets after 32–49 days of storage with no apparent relation to the Microflora or salt content (4.1–6.1% in the water phase). Whole fillets developed the same spoilage characteristics, which included sweet, sour, bitter, faecal, ammonia and cabbage-like off-flavours, as sliced salmon but had a softer texture at the time of rejection. Plate count and Malthus detection times revealed that the Microflora varied in numbers and composition among the three smokehouses. The Microflora differed between fillets and sliced salmon, indicating the impact of the in-house flora on the size and composition of the Microflora. Lactic acid bacteria, Enterobacteriaceae , psychrotrophic marine vibrio and Photobacterium spp. dominated the Microflora of spoiled salmon. Use of conductometric measurements (Malthus) calibrated to traditional agar methods was not a suitable rapid microbiological method for determining the size and composition of the Microflora, because of the variable Microflora.

Simonetta Cirilli - One of the best experts on this subject based on the ideXlab platform.

  • microfloristic provincialism in the upper triassic circum mediterranean area and palaeogeographic implication
    Geobios, 2007
    Co-Authors: Nicoletta Buratti, Simonetta Cirilli
    Abstract:

    Abstract This study represents a contribution to the developing knowledge about the microfloristic provincialism affecting Upper Triassic palynoflora. The compositional differences existing between the Onslow and the Ipswich Microfloras are mainly based on the presence, in the Onslow Microflora, of a diverse and more varied group of gymnosperm pollen grains, including typical European elements. In this study, the palynological assemblages recovered from Carnian successions of the western Tethyan margin (Sicily, Tunisia, Albany, Libya and Israel) are compared with those of West Timor Microfloral assemblages, which have been referred to the Onslow Microflora of southern hemisphere. They contain several common taxa, mainly consisting of conifer miospores widely recorded in Carnian European successions and less frequently recovered in the Carnian of western and eastern Australia. The number of Circum-Mediterranean sporomorphs in the Onslow Microflora assemblages is wider than previously thought, providing new evidences to extend the distribution of the Onslow Microflora to include Carnian associations formerly assigned to the Circum-Mediterranean assemblages. These broad Microfloral affinities seem to indicate the existence of a homogeneous Microflora that maintains, with minor variations, its composition from western Tethys coasts to the northern Australian margin (West Timor). The parent plant community grew in a coastal environment, along the continental margins; the establishment of an equable climatic regime influenced by warm equatorial currents and suitable humid conditions probably conditioned its diffusion. It has long been recognised that the strong floral provinciality which characterised the Late Triassic world gave way to a more homogeneous flora in the Early Jurassic. The decrease in macrofloral diversity is associated with a less pronounced microfloristic provincialism, which in turn coincides with the rise, to strong dominance, of cheirolepidiaceous conifers (Circumpolles producers). This important microfloristic event occurs in the southern hemisphere during the Early Jurassic, however this study reveals their incipient diffusion during the Norian.

  • Microfloristic provincialism in the Upper Triassic Circum-Mediterranean area and palaeogeographic implication
    Geobios, 2007
    Co-Authors: Nicoletta Buratti, Simonetta Cirilli
    Abstract:

    This study represents a contribution to the developing knowledge about the microfloristic provincialism affecting Upper Triassic palynoflora. The compositional differences existing between the Onslow and the Ipswich Microfloras are mainly based on the presence, in the Onslow Microflora, of a diverse and more varied group of gymnosperm pollen grains, including typical European elements. In this study, the palynological assemblages recovered from Carnian successions of the western Tethyan margin (Sicily, Tunisia, Albany, Libya and Israel) are compared with those of West Timor Microfloral assemblages, which have been referred to the Onslow Microflora of southern hemisphere. They contain several common taxa, mainly consisting of conifer miospores widely recorded in Carnian European successions and less frequently recovered in the Carnian of western and eastern Australia. The number of Circum-Mediterranean sporomorphs in the Onslow Microflora assemblages is wider than previously thought, providing new evidences to extend the distribution of the Onslow Microflora to include Carnian associations formerly assigned to the Circum-Mediterranean assemblages. These broad Microfloral affinities seem to indicate the existence of a homogeneous Microflora that maintains, with minor variations, its composition from western Tethys coasts to the northern Australian margin (West Timor). The parent plant community grew in a coastal environment, along the continental margins; the establishment of an equable climatic regime influenced by warm equatorial currents and suitable humid conditions probably conditioned its diffusion. It has long been recognised that the strong floral provinciality which characterised the Late Triassic world gave way to a more homogeneous flora in the Early Jurassic. The decrease in macrofloral diversity is associated with a less pronounced microfloristic provincialism, which in turn coincides with the rise, to strong dominance, of cheirolepidiaceous conifers (Circumpolles producers). This important microfloristic event occurs in the southern hemisphere during the Early Jurassic, however this study reveals their incipient diffusion during the Norian. © 2007 Elsevier Masson SAS. All rights reserved.

  • microfloristic provincialism in the upper triassic circum mediterranean area and palaeogeographic implication provincialisme microfloristique dans les regions circum mediterraneennes et implications paleogeographiques
    2007
    Co-Authors: Nicoletta Buratti, Simonetta Cirilli
    Abstract:

    This study represents a contribution to the developing knowledge about the microfloristic provincialism affecting Upper Triassic palynoflora. The compositional differences existing between the Onslow and the Ipswich Microfloras are mainly based on the presence, in the Onslow Microflora, of a diverse and more varied group of gymnosperm pollen grains, including typical European elements. In this study, the palynological assemblages recovered from Carnian successions of the western Tethyan margin (Sicily, Tunisia, Albany, Libya and Israel) are compared with those of West Timor Microfloral assemblages, which have been referred to the Onslow Microflora of southern hemisphere. They contain several common taxa, mainly consisting of conifer miospores widely recorded in Carnian European successions and less frequently recovered in the Carnian of western and eastern Australia. The number of Circum-Mediterranean sporomorphs in the Onslow Microflora assemblages is wider than previously thought, providing new evidences to extend the distribution of the Onslow Microflora to include Carnian associations formerly assigned to the Circum-Mediterranean assemblages. These broad Microfloral affinities seem to indicate the existence of a homogeneous Microflora that maintains, with minor variations, its composition from western Tethys coasts to the northern Australian margin (West Timor). The parent plant community grew in a coastal environment, along the continental margins; the establishment of an equable climatic regime influenced by warm equatorial currents and suitable humid conditions probably conditioned its diffusion. It has long been recognised that the strong floral provinciality which characterised the Late Triassic world gave way to a more homogeneous flora in the Early Jurassic. The decrease in macrofloral diversity is associated with a less pronounced microfloristic provincialism, which in turn coincides with the rise, to strong dominance, of cheirolepidiaceous conifers (Circumpolles producers). This important microfloristic event occurs in the southern hemisphere during the Early Jurassic, however this study reveals their incipient diffusion during the Norian.

Ingiäld Hafström - One of the best experts on this subject based on the ideXlab platform.

  • Effect of sulphasalazine on gastrointestinal Microflora and on mucosal heat shock protein expression in patients with rheumatoid arthritis
    Br.J Rheumatol., 1994
    Co-Authors: L. Kanerud, Cari Erik Nord, Annika Scheynius, Ingiäld Hafström
    Abstract:

    This study was performed in order to elucidate a possible association between mucosal heat shock protein expression, the gastrointestinal Microflora and disease activity in 17 patients with RA before and after 16 weeks of sulphasalazine (SASP) treatment. The duodenal-jejunal mucosal binding of the monoclonal antibody ML30, recognizing the 65 kDa heat shock protein of mycobacteria, was increased (P = 0.048) in the untreated RA patients compared to controls, but did not correlate to disease, activity or Microflora and was not altered by SASP therapy. There was no convincing evidence for bacterial overgrowth in the jejunum and the faecal Microflora was normal. SASP treatment altered the faecal Microflora, with significant reductions of the total aerobic bacteria, Escherichia coli and Bacteroides, and increased numbers of Bacillus. SASP had only minor effects on the jejunal Microflora. A high carriage frequency of Candida albicans was found in saliva and the counts correlated negatively with the unstimulated whole salivary secretion rate. These results suggest that the gut may be involved in the aetiopathogenesis of RA but do not substantiate the hypothesis that the anti-rheumatic effects of SASP are mediated via its anti-microbial properties. However, the possibility that a micro-organism, not detected in this study, may be of crucial importance in RA, cannot be ruled out

  • Effect of suphasalazine on gastrointestinal Microflora and on mucosal heat shock protein expression in patients with rheumatoid arthritis
    British Journal of Rheumatology, 1994
    Co-Authors: L. Kanerud, Cari Erik Nord, Annika Scheynius, Ingiäld Hafström
    Abstract:

    This study was performed in order to elucidate a possible association between mucosal heat shock protein expression, the gastrointestinal Microflora and disease activity in 17 patients with RA before and after 16 weeks of sulphasalazine (SASP) treatment. The duodenal-jejunal mucosal binding of the monoclonal antibody ML30, recognizing the 65 kDa heat shock protein of mycobacteria, was increased (P = 0.048) in the untreated RA patients compared to controls, but did not correlate to disease activity or Microflora and was not altered by SASP therapy. There was no convincing evidence for bacterial overgrowth in the jejunum and the faecal Microflora was normal SASP treatment altered the faecal Microflora, with significant reductions of the total aerobic bacteria, Escherichia coli and Bacteroides, and increased numbers of Bacillus. SASP had only minor effects on the jejunal Microflora. A high carriage frequency of Candida albicans was found in saliva and the counts correlated negatively with the unstimulated whole salivary secretion rate. These results suggest that the gut may be involved in the aetiopathogenesis of RA but do not substantiate the hypothesis that the anti-rheumatic effects of SASP are mediated via its anti-microbial properties. However, the possibility that a micro-organism, not detected in this study, may be of crucial importance in RA, cannot be ruled out.