The Experts below are selected from a list of 69 Experts worldwide ranked by ideXlab platform

Yijing Li - One of the best experts on this subject based on the ideXlab platform.

  • oral immunization with a lactobacillus casei based anti porcine epidemic diarrhoea virus pedv vaccine expressing Microfold Cell targeting peptide co1 fused with the coe antigen of pedv
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yijing Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

Xiaona Wang - One of the best experts on this subject based on the ideXlab platform.

  • oral immunization with a lactobacillus casei based anti porcine epidemic diarrhoea virus pedv vaccine expressing Microfold Cell targeting peptide co1 fused with the coe antigen of pedv
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yijing Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

  • Oral immunization with a Lactobacillus casei‐based anti‐porcine epidemic diarrhoea virus (PEDV) vaccine expressing Microfold Cell‐targeting peptide Co1 fused with the COE antigen of PEDV
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yang Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

Li Wang - One of the best experts on this subject based on the ideXlab platform.

  • oral immunization with a lactobacillus casei based anti porcine epidemic diarrhoea virus pedv vaccine expressing Microfold Cell targeting peptide co1 fused with the coe antigen of pedv
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yijing Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

  • Oral immunization with a Lactobacillus casei‐based anti‐porcine epidemic diarrhoea virus (PEDV) vaccine expressing Microfold Cell‐targeting peptide Co1 fused with the COE antigen of PEDV
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yang Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

D Z Zheng - One of the best experts on this subject based on the ideXlab platform.

  • oral immunization with a lactobacillus casei based anti porcine epidemic diarrhoea virus pedv vaccine expressing Microfold Cell targeting peptide co1 fused with the coe antigen of pedv
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yijing Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

  • Oral immunization with a Lactobacillus casei‐based anti‐porcine epidemic diarrhoea virus (PEDV) vaccine expressing Microfold Cell‐targeting peptide Co1 fused with the COE antigen of PEDV
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yang Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

Yanping Jiang - One of the best experts on this subject based on the ideXlab platform.

  • oral immunization with a lactobacillus casei based anti porcine epidemic diarrhoea virus pedv vaccine expressing Microfold Cell targeting peptide co1 fused with the coe antigen of pedv
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yijing Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.

  • Oral immunization with a Lactobacillus casei‐based anti‐porcine epidemic diarrhoea virus (PEDV) vaccine expressing Microfold Cell‐targeting peptide Co1 fused with the COE antigen of PEDV
    Journal of Applied Microbiology, 2018
    Co-Authors: Xiaona Wang, Li Wang, D Z Zheng, S Chen, Xinyuan Qiao, Yanping Jiang, Lijie Tang, Yigang Xu, Yang Li
    Abstract:

    Aims The aims of this study were to develop an effective M Cell-targeting oral vaccine, involving Lactobacillus casei to deliver the porcine epidemic diarrhoea virus (PEDV) core neutralizing epitope (COE) antigen conjugated with M Cell-targeting peptide Co1 as an adjuvant, against PEDV infection. Methods and results Genetically engineered L. casei 393 (L393) strains expressing PEDV COE antigen only (pPG-COE/L393) or fused-expressing COE and M Cell-targeting peptide Co1 (pPG-COE-Co1/L393) were constructed, and the immunogenicity upon administration as an oral vaccine was evaluated. The results showed that higher anti-PEDV serum IgG and mucosal SIgA antibody responses were induced in mice orally immunized with strain pPG-COE-Co1/L393 as compared to the mice immunized with strain L393 expressing COE alone or carrying the empty plasmid. In addition, the use of the Co1 ligand elicited a splenocyte proliferative response more effectively in comparison with the COE antigen alone and supported a skewed T helper 2 type of immune response against PEDV. Conclusions pPG-COE-Co1/L393 can effectively induce mucosal, humoural and Th2-type Cellular immune responses against PEDV infection via oral administration. Furthermore, M Cell-targeting peptide ligand Co1 is a good mucosal adjuvant. Significance and impact of the study Lactobacillus casei delivering the COE antigen of PEDV conjugated with a M Cell-targeting peptide Co1 as an immune adjuvant is a promising oral vaccine candidate for PEDV.