The Experts below are selected from a list of 71055 Experts worldwide ranked by ideXlab platform
Ulf Olsson - One of the best experts on this subject based on the ideXlab platform.
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rapid confocal imaging of vesicle to sponge phase droplet transition in dilute dispersions of the c 10 e 3 surfactant
Scientific Reports, 2019Co-Authors: Andre P Schroder, Jerome J Crassous, Carlos M Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3 ) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
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Rapid confocal imaging of vesicle-to-sponge phase droplet transition in dilute dispersions of the C10E3 surfactant
Scientific Reports, 2019Co-Authors: André Schroder, Jerome J Crassous, Carlos Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter-and intra-lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
Jerome J Crassous - One of the best experts on this subject based on the ideXlab platform.
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rapid confocal imaging of vesicle to sponge phase droplet transition in dilute dispersions of the c 10 e 3 surfactant
Scientific Reports, 2019Co-Authors: Andre P Schroder, Jerome J Crassous, Carlos M Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3 ) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
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Rapid confocal imaging of vesicle-to-sponge phase droplet transition in dilute dispersions of the C10E3 surfactant
Scientific Reports, 2019Co-Authors: André Schroder, Jerome J Crassous, Carlos Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter-and intra-lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
Robert M Graham - One of the best experts on this subject based on the ideXlab platform.
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insulin like growth factor igf i induces myotube hypertrophy associated with an increase in anaerobic glycolysis in a clonal skeletal muscle cell model
Biochemical Journal, 1999Co-Authors: Christopher Semsarian, Pramod Sutrave, David R Richmond, Robert M GrahamAbstract:Insulin-like growth factor-I (IGF-I) is an important autocrine/paracrine mediator of skeletal-muscle growth and development. To develop a definitive cultured cell model of skeletal-muscle hypertrophy, C2C12 cells were stably transfected with IGF-I and clonal lines developed and evaluated. Quantitative morphometric analysis showed that IGF-I-transfected myotubes had a larger area (2381+/-60 Micrometer2 versus 1429+/-39 Micrometer2; P<0.0001) and a greater maximum width (21.4+/-0.6 Micrometer versus 13.9+/-0.3 Micrometer; P<0.0001) than control C2C12 myotubes, independent of the number of cell nuclei per myotube. IGF-I-transfected myotubes had higher levels of protein synthesis but no difference in DNA synthesis when compared with control myotubes, indicating the development of hypertrophy rather than hyperplasia. Both lactate dehydrogenase and alanine aminotransferase activities were increased (3- and 5-fold respectively), and total lactate levels were higher (2.3-fold) in IGF-I-transfected compared with control myotubes, indicating an increase in anaerobic glycolysis in the hypertrophied myotubes. However, expression of genes involved in skeletal-muscle growth or hypertrophy in vivo, e.g. myocyte nuclear factor and myostatin, was not altered in the IGF-I myotubes. Finally, myotube hypertrophy could also be induced by treatment of C2C12 cells with recombinant IGF-I or by growing C2C12 cells in conditioned media from IGF-I-transfected cells. This quantitative model should be uniquely useful for elucidating the molecular mechanisms of skeletal-muscle hypertrophy.
André Schroder - One of the best experts on this subject based on the ideXlab platform.
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Rapid confocal imaging of vesicle-to-sponge phase droplet transition in dilute dispersions of the C10E3 surfactant
Scientific Reports, 2019Co-Authors: André Schroder, Jerome J Crassous, Carlos Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter-and intra-lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
Andre P Schroder - One of the best experts on this subject based on the ideXlab platform.
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rapid confocal imaging of vesicle to sponge phase droplet transition in dilute dispersions of the c 10 e 3 surfactant
Scientific Reports, 2019Co-Authors: Andre P Schroder, Jerome J Crassous, Carlos M Marques, Ulf OlssonAbstract:The lamellar-to-sponge phase transition of fluorescently labelled large unilamellar vesicles (LUVs) of the non-ionic surfactant triethylene glycol mono n-decyl ether (C 10 E 3 ) was investigated in situ by confocal laser scanning microscopy (CLSM). Stable dispersions of Micrometer-sized C 10 E 3 LUVs were prepared at 20 °C and quickly heated at different temperatures close to the lamellar-to-sponge phase transition temperature. Phase transition of the strongly fluctuating individual vesicles into micrometre-sized sponge phase droplets was observed to occur via manyfold multilamellar morphologies with increasing membrane confinement through inter- and intra- lamellar fusion. The very low bending rigidity and lateral tension of the C 10 E 3 bilayer were supported by quantitative image analysis of a stable fluctuating membrane using both flicker noise spectroscopy and spatial autocorrelation function.
