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Nanette H. Bishopric - One of the best experts on this subject based on the ideXlab platform.

  • microRNA-20a Constrains p300-Driven Myocardial Angiogenic Transcription by Direct Targeting of p300
    2016
    Co-Authors: Lina A. Shehadeh, Salil Sharma, Jian Qin Wei, Jing Liu, Huijun Yuan, Claudia O. Rodrigues, Michaela Scherr, Nicholas F. Tsinoremas, Nanette H. Bishopric
    Abstract:

    Objective: To characterize downstream effectors of p300 acetyltransferase in the myocardium. Background: Acetyltransferase p300 is a central driver of the hypertrophic response to increased workload, but its biological targets and downstream effectors are incompletely known. Methods and Results: Mice expressing a myocyte-restricted transgene encoding acetyltransferase p300, previously shown to develop spontaneous hypertrophy, were observed to undergo robust compensatory blood vessel growth together with increased angiogenic gene expression. Chromatin immunoprecipitation demonstrated binding of p300 to the enhancers of the angiogenic regulators Angpt1 and Egln3. Interestingly, p300 overexpression in vivo was also associated with relative upregulation of several members of the anti-angiogenic miR-17,92 cluster in vivo. Confirming this finding, both miR-17-3p and miR-20a were upregulated in neonatal rat ventricular myocytes following adenoviral transduction of p300. Relative expression of most members of the 17,92 cluster was similar in all 4 cardiac chambers and in other organs, however, significant downregulation of miR-17-3p and miR-20a occurred between 1 and 8 months of age in both wt and tg mice. The decline in expression of these microRNAs was associated with increased expression of VEGFA, a validated miR-20a target. In addition, miR-20a was demonstrated to directly repress p300 expression through a consensus binding site in the p30

  • microRNA 20a constrains p300 driven myocardial angiogenic transcription by direct targeting of p300
    PLOS ONE, 2013
    Co-Authors: Lina A. Shehadeh, Salil Sharma, Jian Qin Wei, Jing Liu, Huijun Yuan, Claudia O. Rodrigues, Michaela Scherr, Nicholas F. Tsinoremas, Monica Pessanha, Nanette H. Bishopric
    Abstract:

    Objective To characterize downstream effectors of p300 acetyltransferase in the myocardium.

Yunfei Bian - One of the best experts on this subject based on the ideXlab platform.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3' untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE-/- mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1 molecular and cell biology of lipids
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3′ untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE⁻/⁻ mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • gw26 e1038 microRNA 20a b regulates cholesterol efflux through posttranscriptional repression of atp binding cassette transporter a1 in thp 1 macrophage raw 264 7 derived foam cells
    Journal of the American College of Cardiology, 2015
    Co-Authors: Bin Liang, Xin Wang, Rui Bai, Huiyu Yang, Zhiming Yang, Yunfei Bian, Yaoxia Han, Chuanshi Xiao
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and has anti-atherosclerosis effects. It has been reported that microRNAs regulate the expression of ABCA1. Recent studies have shown that microRNA (miR)-20a/b might play an important role in

Chuanshi Xiao - One of the best experts on this subject based on the ideXlab platform.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3' untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE-/- mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1 molecular and cell biology of lipids
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3′ untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE⁻/⁻ mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • gw26 e1038 microRNA 20a b regulates cholesterol efflux through posttranscriptional repression of atp binding cassette transporter a1 in thp 1 macrophage raw 264 7 derived foam cells
    Journal of the American College of Cardiology, 2015
    Co-Authors: Bin Liang, Xin Wang, Rui Bai, Huiyu Yang, Zhiming Yang, Yunfei Bian, Yaoxia Han, Chuanshi Xiao
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and has anti-atherosclerosis effects. It has been reported that microRNAs regulate the expression of ABCA1. Recent studies have shown that microRNA (miR)-20a/b might play an important role in

Bin Liang - One of the best experts on this subject based on the ideXlab platform.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3' untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE-/- mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1 molecular and cell biology of lipids
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3′ untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE⁻/⁻ mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • gw26 e1038 microRNA 20a b regulates cholesterol efflux through posttranscriptional repression of atp binding cassette transporter a1 in thp 1 macrophage raw 264 7 derived foam cells
    Journal of the American College of Cardiology, 2015
    Co-Authors: Bin Liang, Xin Wang, Rui Bai, Huiyu Yang, Zhiming Yang, Yunfei Bian, Yaoxia Han, Chuanshi Xiao
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and has anti-atherosclerosis effects. It has been reported that microRNAs regulate the expression of ABCA1. Recent studies have shown that microRNA (miR)-20a/b might play an important role in

Xin Wang - One of the best experts on this subject based on the ideXlab platform.

  • microRNA 20a 5p ameliorates non alcoholic fatty liver disease via inhibiting the expression of cd36
    Frontiers in Cell and Developmental Biology, 2020
    Co-Authors: Xin Wang, Longyan Yang, Dong Zhao
    Abstract:

    Fatty acid translocase CD36 (CD36) plays an important role in the initiation and pathogenesis of chronic liver disease and non-alcoholic fatty liver disease (NAFLD). The purpose of this study is to investigate the regulation of microRNA-20a-5p (miR-20a-5p) on CD36 in the pathogenesis of NAFLD. Human plasma samples were obtained from NAFLD patients and healthy controls. Mice were fed with high-fat diet to induce an in vivo NAFLD model. Histology staining was performed to examine the morphology and lipid deposition of mouse liver tissue. Real-time PCR, dual-luciferase assay, and western blotting were employed to detect the relationship between miR-20a-5p and CD36. The expression level of miR-20a-5p was decreased in NAFLD patients, HFD mice, and free fatty acid (FFA)-treated HepG2 cells or primary mouse hepatocytes, accompanied by increased lipid production in hepatocytes. MiR-20a-5p suppressed the expression of CD36 to reduce lipid accumulation via binding to its 3'-untranslated region (UTR). However, under the condition of interference with CD36, further inhibition of miR-20a-5p would not cause lipid over-accumulation. In this study, we found that miR-20a-5p played a protective role in lipid metabolic disorders of NAFLD by targeting CD36, which indicated the prospect of miR-20a-5p as a biomarker and treatment target for NAFLD.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3' untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE-/- mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • microRNA 20a b regulates cholesterol efflux through post transcriptional repression of atp binding cassette transporter a1 molecular and cell biology of lipids
    Biochimica et Biophysica Acta, 2017
    Co-Authors: Bin Liang, Xin Wang, Xiaosu Song, Rui Bai, Huiyu Yang, Zhiming Yang, Chuanshi Xiao, Yunfei Bian
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and exhibits anti-atherosclerosis effects. Some microRNAs (miRs) regulate ABCA1 expression, and recent studies have shown that miR-20a/b might play a critical role in atherosclerotic diseases. Here, we attempted to clarify the potential contribution of miR-20a/b in post-transcriptional regulation of ABCA1, cholesterol efflux, and atherosclerosis. We performed bioinformatics analysis and found that miR-20a/b was highly conserved and directly bound to ABCA1 mRNA with low binding free energy. Luciferase-reporter assay also confirmed that miR-20a/b significantly reduced luciferase activity associated with the ABCA1 3′ untranslated region reporter construct. Additionally, miR-20a/b decreased ABCA1 expression, which, in turn, decreased cholesterol efflux and increased cholesterol content in THP-1 and RAW 264.7 macrophage-derived foam cells. In contrast, miR-20a/b inhibitors increased ABCA1 expression and cholesterol efflux, decreased cholesterol content, and inhibited foam-cell formation. Consistent with our in vitro results, miR-20a/b-treated ApoE⁻/⁻ mice showed decreased ABCA1expression in the liver and reductions of reverse cholesterol transport in vivo. Furthermore, miR-20a/b regulated the formation of nascent high-density lipoprotein and promoted atherosclerotic development, whereas miR-20a/b knockdown attenuated atherosclerotic formation. miR-20 is a new miRNA capable of targeting ABCA1 and regulating ABCA1 expression. Therefore, miR-20 inhibition constitutes a new strategy for ABCA1-based treatment of atherosclerosis.

  • gw26 e1038 microRNA 20a b regulates cholesterol efflux through posttranscriptional repression of atp binding cassette transporter a1 in thp 1 macrophage raw 264 7 derived foam cells
    Journal of the American College of Cardiology, 2015
    Co-Authors: Bin Liang, Xin Wang, Rui Bai, Huiyu Yang, Zhiming Yang, Yunfei Bian, Yaoxia Han, Chuanshi Xiao
    Abstract:

    ATP-binding cassette transporter A1 (ABCA1) plays a crucial role in reverse cholesterol transport and has anti-atherosclerosis effects. It has been reported that microRNAs regulate the expression of ABCA1. Recent studies have shown that microRNA (miR)-20a/b might play an important role in