The Experts below are selected from a list of 311400 Experts worldwide ranked by ideXlab platform
Brenda E Porter - One of the best experts on this subject based on the ideXlab platform.
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changes in microRNA expression in the whole hippocampus and hippocampal synaptoneurosome fraction following pilocarpine induced status epilepticus
PLOS ONE, 2013Co-Authors: Rashmi M Risbud, Brenda E PorterAbstract:microRNAs regulate protein synthesis by binding non-translated regions of mRNAs and suppressing translation and/or increasing mRNA degradation. microRNAs play an important role in the nervous system including controlling synaptic plasticity. Their expression is altered in disease states including stroke, head injury and epilepsy. To better understand microRNA expression changes that might contribute to the development of epilepsy, microRNA arrays were performed on rat hippocampus 4 hours, 48 hours and 3 weeks following an episode of pilocarpine induced status epilepticus. Eighty microRNAs increased at one or more of the time points. No microRNAs decreased at 4 hours, and only a few decreased at 3 weeks, but 188 decreased 48 hours after status epilepticus. The large number of microRNAs with altered expression following status epilepticus suggests that microRNA regulation of translation has the potential to contribute to changes in protein expression during epileptogenesis. We carried out a second set of array’s comparing microRNA expression at 48 hours in synaptoneurosome and nuclear fractions of the hippocampus. In control rat hippocampi multiple microRNAs were enriched in the synaptoneurosomal fraction as compared to the nuclear fraction. In contrast, 48 hours after status epilepticus only one microRNA was enriched in the synaptoneurosome fraction. The loss of microRNAs enriched in the synaptoneurosomal fraction implies a dramatic change in translational regulation in synapses 48 hours after status epilepticus.
Bo Xiao - One of the best experts on this subject based on the ideXlab platform.
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expression profile of microRNAs in rat hippocampus following lithium pilocarpine induced status epilepticus
Neuroscience Letters, 2011Co-Authors: Kai Hu, Chen Zhang, Lili Long, Xiaoyan Long, Li Feng, Yi Li, Bo XiaoAbstract:Abstract Although microRNAs are expressed extensively in the central nervous system in physiological and pathological conditions, their expression in neurological disorder of epilepsy has not been well characterized. Here we investigated microRNA expression pattern in post status epilepticus rats (24 h after status). Rat microRNA array and differential analysis had detected 19 up-regulated microRNAs and 7 down-regulated microRNAs in rat hippocampus, and four randomly selected deregulated microRNAs (microRNA-34a, microRNA-22, microRNA-125a, microRNA-21) were confirmed by qRT-PCR, then their expression alterations in rat peripheral blood were analyzed. We found that these four deregulated microRNAs were also differentially expressed in rat peripheral blood, and trends for their blood expression alterations were just the same as their counterparts in rat hippocampus. Thus, our results have not only characterized the microRNA expression profile in post status epilepticus rat hippocampus but also demonstrated that some rat hippocampal microRNAs were probably associated with rat peripheral blood microRNAs. Moreover, targets of these deregulated microRNAs were analyzed using bioinformatics and the identified enriched MAPK pathway and long-term potentiation pathway might have been involved in molecular mechanisms concerning neuronal death, inflammation and epileptogenesis.
Gang Cheng - One of the best experts on this subject based on the ideXlab platform.
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a naturally derived dextran peptide vector for microRNA antagomir delivery
RSC Advances, 2015Co-Authors: Qiong Tang, Yanqiao Zhang, Gang ChengAbstract:Single stranded microRNAs and their antagomirs are unstable and polyanionic, which impedes efficient cellular uptake and reduces half-life. Therefore, effective delivery systems with low toxicity for microRNAs are urgently needed for the success of microRNA-based therapy. Here, a dextran–peptide hybrid, Dex10-R5H5(40%), was developed as a carrier to deliver microRNAs. Dex10-R5H5(40%) loaded with antagomir-149 could reduce the level of endogenous microRNA-149 by 76% and it is more effective than the commercially available transfection reagent, RNAiMAX, which leads to 67% reduction. Additionally, Dex10-R5H5(40%) exhibited no cytotoxicity to HepG2 cells. These results indicate that the dextran–peptide hybrid may be a promising delivery system for the safe and efficient microRNA-based therapy.
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A naturally derived dextran–peptide vector for microRNA antagomir delivery
RSC Advances, 2015Co-Authors: Qiong Tang, Yanqiao Zhang, Gang ChengAbstract:Single stranded microRNAs and their antagomirs are unstable and polyanionic, which impedes efficient cellular uptake and reduces half-life. Therefore, effective delivery systems with low toxicity for microRNAs are urgently needed for the success of microRNA-based therapy. Here, a dextran–peptide hybrid, Dex10-R5H5(40%), was developed as a carrier to deliver microRNAs. Dex10-R5H5(40%) loaded with antagomir-149 could reduce the level of endogenous microRNA-149 by 76% and it is more effective than the commercially available transfection reagent, RNAiMAX, which leads to 67% reduction. Additionally, Dex10-R5H5(40%) exhibited no cytotoxicity to HepG2 cells. These results indicate that the dextran–peptide hybrid may be a promising delivery system for the safe and efficient microRNA-based therapy.
Rashmi M Risbud - One of the best experts on this subject based on the ideXlab platform.
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changes in microRNA expression in the whole hippocampus and hippocampal synaptoneurosome fraction following pilocarpine induced status epilepticus
PLOS ONE, 2013Co-Authors: Rashmi M Risbud, Brenda E PorterAbstract:microRNAs regulate protein synthesis by binding non-translated regions of mRNAs and suppressing translation and/or increasing mRNA degradation. microRNAs play an important role in the nervous system including controlling synaptic plasticity. Their expression is altered in disease states including stroke, head injury and epilepsy. To better understand microRNA expression changes that might contribute to the development of epilepsy, microRNA arrays were performed on rat hippocampus 4 hours, 48 hours and 3 weeks following an episode of pilocarpine induced status epilepticus. Eighty microRNAs increased at one or more of the time points. No microRNAs decreased at 4 hours, and only a few decreased at 3 weeks, but 188 decreased 48 hours after status epilepticus. The large number of microRNAs with altered expression following status epilepticus suggests that microRNA regulation of translation has the potential to contribute to changes in protein expression during epileptogenesis. We carried out a second set of array’s comparing microRNA expression at 48 hours in synaptoneurosome and nuclear fractions of the hippocampus. In control rat hippocampi multiple microRNAs were enriched in the synaptoneurosomal fraction as compared to the nuclear fraction. In contrast, 48 hours after status epilepticus only one microRNA was enriched in the synaptoneurosome fraction. The loss of microRNAs enriched in the synaptoneurosomal fraction implies a dramatic change in translational regulation in synapses 48 hours after status epilepticus.
Kai Hu - One of the best experts on this subject based on the ideXlab platform.
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expression profile of microRNAs in rat hippocampus following lithium pilocarpine induced status epilepticus
Neuroscience Letters, 2011Co-Authors: Kai Hu, Chen Zhang, Lili Long, Xiaoyan Long, Li Feng, Yi Li, Bo XiaoAbstract:Abstract Although microRNAs are expressed extensively in the central nervous system in physiological and pathological conditions, their expression in neurological disorder of epilepsy has not been well characterized. Here we investigated microRNA expression pattern in post status epilepticus rats (24 h after status). Rat microRNA array and differential analysis had detected 19 up-regulated microRNAs and 7 down-regulated microRNAs in rat hippocampus, and four randomly selected deregulated microRNAs (microRNA-34a, microRNA-22, microRNA-125a, microRNA-21) were confirmed by qRT-PCR, then their expression alterations in rat peripheral blood were analyzed. We found that these four deregulated microRNAs were also differentially expressed in rat peripheral blood, and trends for their blood expression alterations were just the same as their counterparts in rat hippocampus. Thus, our results have not only characterized the microRNA expression profile in post status epilepticus rat hippocampus but also demonstrated that some rat hippocampal microRNAs were probably associated with rat peripheral blood microRNAs. Moreover, targets of these deregulated microRNAs were analyzed using bioinformatics and the identified enriched MAPK pathway and long-term potentiation pathway might have been involved in molecular mechanisms concerning neuronal death, inflammation and epileptogenesis.
