The Experts below are selected from a list of 1892880 Experts worldwide ranked by ideXlab platform
Dylan M Owen - One of the best experts on this subject based on the ideXlab platform.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision. Achieving high axial resolution is challenging in single-molecule localization Microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
Alan M Szalai - One of the best experts on this subject based on the ideXlab platform.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision. Achieving high axial resolution is challenging in single-molecule localization Microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
Bruno Siarry - One of the best experts on this subject based on the ideXlab platform.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision. Achieving high axial resolution is challenging in single-molecule localization Microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
Damian Refojo - One of the best experts on this subject based on the ideXlab platform.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision. Achieving high axial resolution is challenging in single-molecule localization Microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
Alfredo Caceres - One of the best experts on this subject based on the ideXlab platform.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule’s image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision. Achieving high axial resolution is challenging in single-molecule localization Microscopy. Here, the authors present a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope without hardware modification, and show nearly isotropic nanometric resolution.
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three dimensional total internal reflection fluorescence nanoscopy with nanometric axial resolution by photometric localization of single molecules
Nature Communications, 2021Co-Authors: Alan M Szalai, Bruno Siarry, Jeronimo Lukin, David J Williamson, Nicolas Unsain, Alfredo Caceres, Mauricio Pilopais, Guillermo P Acuna, Damian Refojo, Dylan M OwenAbstract:Single-molecule localization Microscopy enables far-field imaging with lateral resolution in the range of 10 to 20 nanometres, exploiting the fact that the centre position of a single-molecule's image can be determined with much higher accuracy than the size of that image itself. However, attaining the same level of resolution in the axial (third) dimension remains challenging. Here, we present Supercritical Illumination Microscopy Photometric z-Localization with Enhanced Resolution (SIMPLER), a photometric method to decode the axial position of single molecules in a total internal reflection fluorescence microscope. SIMPLER requires no hardware modification whatsoever to a conventional total internal reflection fluorescence microscope and complements any 2D single-molecule localization Microscopy method to deliver 3D images with nearly isotropic nanometric resolution. Performance examples include SIMPLER-direct stochastic optical reconstruction Microscopy images of the nuclear pore complex with sub-20 nm axial localization precision and visualization of microtubule cross-sections through SIMPLER-DNA points accumulation for imaging in nanoscale topography with sub-10 nm axial localization precision.
