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Anna Brillowskadąbrowska - One of the best experts on this subject based on the ideXlab platform.

  • pcr and real time pcr approaches to the identification of arthroderma otae species Microsporum canis and Microsporum Audouinii Microsporum ferrugineum
    Journal of The European Academy of Dermatology and Venereology, 2016
    Co-Authors: Natalia Kobylak, Barbara Bykowska, Roman Nowicki, Ewelina Kurzyk, Anna Brillowskadąbrowska
    Abstract:

    Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum Audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. Audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. Audouinii/M. ferrugineum DNA from a pure culture.

Yvonne Gräser - One of the best experts on this subject based on the ideXlab platform.

  • Unusual strains of Microsporum Audouinii causing tinea in Europe
    Mycoses, 2015
    Co-Authors: Jochen Brasch, S Müller, Yvonne Gräser
    Abstract:

    We comment on an unusual strain of Microsporum (M.) Audouinii. It was isolated from tinea corporis of a boy who lived in Germany and most likely had acquired his infection during a stay on a farm with animal husbandry in Poland. The strain showed features of M. canis (plenty of markedly rough-walled macroconidia, growth on rice, positive hair perforation) as well as of M. Audouinii (white thallus, long macroconidia with central constriction) and in vitro it degraded hair of various mammals. Because its ribosomal internal transcribed spacer region showed 99.9% homology to a M. Audouinii reference strain it was finally identified as M. Audouinii. We relate these findings with recent observations of M. Audouinii causing tinea in Europe. This appraisal suggests that irrespective of an identical ribosomal ITS region distinct M. Audouinii strains can display a spectrum of morphological and physiological features that is broader than currently outlined in mycological textbooks. Certain unusual characteristics like an enhanced capacity to utilise keratins may even be associated with unexpected transmission routes. Above all sporadic M. Audouinii infections in Europe that bear no relation to an endemic area should be analysed from this perspective.

  • Tinea corporis caused by an unusual strain of Microsporum Audouinii that perforates hair in vitro
    Mycoses, 2009
    Co-Authors: Jochen Brasch, R. Hügel, F. Lipowsky, Yvonne Gräser
    Abstract:

    We report on a dermatophyte infection acquired by a young woman from Germany who had worked in Ghana. The strain isolated from her skin lesions showed morphological and physiological features compatible with Microsporum Audouinii but a clearly positive hair perforation test made its definite identification by conventional methods equivocal. A genetic analysis finally unambiguously revealed Microsporum Audouinii. This is the first observation of a Microsporum Audouinii strain with a positive hair perforation test. The ability to perforate hair may be related to attributes favouring an inflammatory host response.

Natalia Kobylak - One of the best experts on this subject based on the ideXlab platform.

  • pcr and real time pcr approaches to the identification of arthroderma otae species Microsporum canis and Microsporum Audouinii Microsporum ferrugineum
    Journal of The European Academy of Dermatology and Venereology, 2016
    Co-Authors: Natalia Kobylak, Barbara Bykowska, Roman Nowicki, Ewelina Kurzyk, Anna Brillowskadąbrowska
    Abstract:

    Objectives The identification of species in the Arthroderma otae complex is essential to determine the origin of infection and to eliminate the risk of transmission. Microsporum canis is a zoophilic species, whereas Microsporum Audouinii and Microsporum ferrugineum are anthropophilic species. In this paper, we propose alternative methods that permit species-specific identification of both anthropophilic and zoophilic members of the A. otae complex Methods Two PCR assays were designed based on differences in the DNA fragment encoding β-tubulin and were applied in both traditional and real-time PCR using DNA isolated by rapid method from culture. Result The two assays presented in this study enable the identification of M. canis and M. Audouinii/M. ferrugineum with 100% sensitivity and specificity by both traditional and real-time PCR. Conclusion We developed a new diagnostic assay using specific primers and both traditional and real-time PCR reactions that can be applied in routine laboratory praxis as well as in epidemiological studies to detect M. canis and M. Audouinii/M. ferrugineum DNA from a pure culture.

Jochen Brasch - One of the best experts on this subject based on the ideXlab platform.

  • Unusual strains of Microsporum Audouinii causing tinea in Europe
    Mycoses, 2015
    Co-Authors: Jochen Brasch, S Müller, Yvonne Gräser
    Abstract:

    We comment on an unusual strain of Microsporum (M.) Audouinii. It was isolated from tinea corporis of a boy who lived in Germany and most likely had acquired his infection during a stay on a farm with animal husbandry in Poland. The strain showed features of M. canis (plenty of markedly rough-walled macroconidia, growth on rice, positive hair perforation) as well as of M. Audouinii (white thallus, long macroconidia with central constriction) and in vitro it degraded hair of various mammals. Because its ribosomal internal transcribed spacer region showed 99.9% homology to a M. Audouinii reference strain it was finally identified as M. Audouinii. We relate these findings with recent observations of M. Audouinii causing tinea in Europe. This appraisal suggests that irrespective of an identical ribosomal ITS region distinct M. Audouinii strains can display a spectrum of morphological and physiological features that is broader than currently outlined in mycological textbooks. Certain unusual characteristics like an enhanced capacity to utilise keratins may even be associated with unexpected transmission routes. Above all sporadic M. Audouinii infections in Europe that bear no relation to an endemic area should be analysed from this perspective.

  • Tinea corporis caused by an unusual strain of Microsporum Audouinii that perforates hair in vitro
    Mycoses, 2009
    Co-Authors: Jochen Brasch, R. Hügel, F. Lipowsky, Yvonne Gräser
    Abstract:

    We report on a dermatophyte infection acquired by a young woman from Germany who had worked in Ghana. The strain isolated from her skin lesions showed morphological and physiological features compatible with Microsporum Audouinii but a clearly positive hair perforation test made its definite identification by conventional methods equivocal. A genetic analysis finally unambiguously revealed Microsporum Audouinii. This is the first observation of a Microsporum Audouinii strain with a positive hair perforation test. The ability to perforate hair may be related to attributes favouring an inflammatory host response.

Macit Ilkit - One of the best experts on this subject based on the ideXlab platform.

  • Identification of rare macroconidia-producing dermatophytic fungi by real-time PCR.
    Medical mycology, 2011
    Co-Authors: Tuba Yuksel, Macit Ilkit
    Abstract:

    To understand the pathogenicity and clinical significance of dermatophytes (also known as ringworms), the correct identification of these molds is essential. However, in routine practice they are notoriously difficult to classify and identify. The morphology of macroconidia, which are abundantly produced under suitable in vitro conditions, have provided useful criteria for the identification of many of the dermatophytes. However, several of them, including Microsporum Audouinii, M. ferrugineum, Trichophyton concentricum, T. schoenleinii, T. verrucosum, and T. violaceum (including T. soudanense and T. yaoundei) rarely produce macroconidia and cannot be easily identified. The objective of this study was to design, optimize, and evaluate real-time PCR as a tool for identifying dermatophytic fungi in a laboratory setting. The performance of the assay was evaluated using 64 dermatophyte isolates, i.e., 35 rare macroconidia-producing reference strains, including the six species mentioned above, and 29 clinical ...