The Experts below are selected from a list of 3846 Experts worldwide ranked by ideXlab platform

Abdul Mannan - One of the best experts on this subject based on the ideXlab platform.

  • phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of trillium govanianum
    Natural Product Research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    : Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Trillium govanianum and its solid phase extraction (SPE) fractions by using resazurin Microtiter Assay (REMA) against Gram positive and Gram negative bacterial registered strains and to carry out phytochemical analysis. The remarkable amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was manifested by MeOH extract (20.27 ± 3.03 mg GAE/g DW and 9.25 ± 0.50 mg QE/g DW respectively). The GC/MS analysis revealed the presence saturated and unsaturated components. Considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against Gram-negative bacteria (MIC: 2.5-0.165 mg/mL) were observed. The use of Microtiter Plates has the advantage of lower cost, fast and quantitative results.

  • Phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of Trillium govanianum.
    Natural product research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to de...

  • evaluation of resazurin Microtiter Plate Assay and hplc photodiode array analysis of the roots of asparagus adscendens
    Natural Product Research, 2018
    Co-Authors: Kashif Maqbool Khan, Ghazanfar Ali Khan, Abdul Mannan, Lutfun Nahar, Muhammad Arfan, Glyn Hobbs, Satyajit D Sarker
    Abstract:

    AbstractAsparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of A. adscendens and its solid-phase extraction (SPE) fractions using resazurin microtitre Assay against Gram-positive and negative bacterial-registered strains and to carry out HPLC-photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5–0.009 mg/mL) than against Gram-negative bacteria (MIC: 1.25–2.5 mg/mL). The use of microtitre Plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.

Otto S. Wolfbeis - One of the best experts on this subject based on the ideXlab platform.

  • Microtiter Plate Assay for phosphate using a europium tetracycline complex as a sensitive luminescent probe
    Analytica Chimica Acta, 2006
    Co-Authors: Axel Duerkop, Matejka Turel, Aleksandra Lobnik, Otto S. Wolfbeis
    Abstract:

    A new luminescent europium probe is presented for the determination of phosphate (P) in Microtiter Plate format. The Assay is based on the quenching of the luminescence of the europium–tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L.

  • Microtiter Plate Assay for phosphate using a europium–tetracycline complex as a sensitive luminescent probe
    Analytica Chimica Acta, 2006
    Co-Authors: Axel Duerkop, Matejka Turel, Aleksandra Lobnik, Otto S. Wolfbeis
    Abstract:

    A new luminescent europium probe is presented for the determination of phosphate (P) in Microtiter Plate format. The Assay is based on the quenching of the luminescence of the europium–tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L.

Kashif Maqbool Khan - One of the best experts on this subject based on the ideXlab platform.

  • phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of trillium govanianum
    Natural Product Research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    : Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Trillium govanianum and its solid phase extraction (SPE) fractions by using resazurin Microtiter Assay (REMA) against Gram positive and Gram negative bacterial registered strains and to carry out phytochemical analysis. The remarkable amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was manifested by MeOH extract (20.27 ± 3.03 mg GAE/g DW and 9.25 ± 0.50 mg QE/g DW respectively). The GC/MS analysis revealed the presence saturated and unsaturated components. Considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against Gram-negative bacteria (MIC: 2.5-0.165 mg/mL) were observed. The use of Microtiter Plates has the advantage of lower cost, fast and quantitative results.

  • Phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of Trillium govanianum.
    Natural product research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to de...

  • evaluation of resazurin Microtiter Plate Assay and hplc photodiode array analysis of the roots of asparagus adscendens
    Natural Product Research, 2018
    Co-Authors: Kashif Maqbool Khan, Ghazanfar Ali Khan, Abdul Mannan, Lutfun Nahar, Muhammad Arfan, Glyn Hobbs, Satyajit D Sarker
    Abstract:

    AbstractAsparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of A. adscendens and its solid-phase extraction (SPE) fractions using resazurin microtitre Assay against Gram-positive and negative bacterial-registered strains and to carry out HPLC-photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5–0.009 mg/mL) than against Gram-negative bacteria (MIC: 1.25–2.5 mg/mL). The use of microtitre Plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.

Satyajit D Sarker - One of the best experts on this subject based on the ideXlab platform.

  • phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of trillium govanianum
    Natural Product Research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    : Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of Trillium govanianum and its solid phase extraction (SPE) fractions by using resazurin Microtiter Assay (REMA) against Gram positive and Gram negative bacterial registered strains and to carry out phytochemical analysis. The remarkable amount of gallic acid equivalent phenolic and quercetin equivalent flavonoid content was manifested by MeOH extract (20.27 ± 3.03 mg GAE/g DW and 9.25 ± 0.50 mg QE/g DW respectively). The GC/MS analysis revealed the presence saturated and unsaturated components. Considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5-0.009 mg/mL) than against Gram-negative bacteria (MIC: 2.5-0.165 mg/mL) were observed. The use of Microtiter Plates has the advantage of lower cost, fast and quantitative results.

  • Phytochemical profiling and evaluation of modified resazurin Microtiter Plate Assay of the roots of Trillium govanianum.
    Natural product research, 2019
    Co-Authors: Kashif Maqbool Khan, Satyajit D Sarker, Ghazanfar Ali Khan, Hammad Saleem, Shujaat Ali Khan, Abdul Mannan
    Abstract:

    Trillium govanianum Wall. ex D. Don (Melanthiaceae alt. Trilliaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to de...

  • evaluation of resazurin Microtiter Plate Assay and hplc photodiode array analysis of the roots of asparagus adscendens
    Natural Product Research, 2018
    Co-Authors: Kashif Maqbool Khan, Ghazanfar Ali Khan, Abdul Mannan, Lutfun Nahar, Muhammad Arfan, Glyn Hobbs, Satyajit D Sarker
    Abstract:

    AbstractAsparagus adscendens Roxb. (Asparagaceae), is native to the Himalayas. The present study, for the first time, was undertaken to explore the antimicrobial potential, to determine the minimum inhibitory concentration (MIC) values of the methanol extract of the roots of A. adscendens and its solid-phase extraction (SPE) fractions using resazurin microtitre Assay against Gram-positive and negative bacterial-registered strains and to carry out HPLC-photodiode array analysis of the SPE fractions. The methanol extract and all SPE exhibited considerable level of antibacterial potential against Gram-positive bacteria (MIC: 2.5–0.009 mg/mL) than against Gram-negative bacteria (MIC: 1.25–2.5 mg/mL). The use of microtitre Plates has the advantage of lower cost, fast and quantitative results. Like other Asparagus species, the presence of phenolic compounds in all SPE fractions was evident in the HPLC-PDA data.

Matejka Turel - One of the best experts on this subject based on the ideXlab platform.

  • Microtiter Plate Assay for phosphate using a europium tetracycline complex as a sensitive luminescent probe
    Analytica Chimica Acta, 2006
    Co-Authors: Axel Duerkop, Matejka Turel, Aleksandra Lobnik, Otto S. Wolfbeis
    Abstract:

    A new luminescent europium probe is presented for the determination of phosphate (P) in Microtiter Plate format. The Assay is based on the quenching of the luminescence of the europium–tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L.

  • Microtiter Plate Assay for phosphate using a europium–tetracycline complex as a sensitive luminescent probe
    Analytica Chimica Acta, 2006
    Co-Authors: Axel Duerkop, Matejka Turel, Aleksandra Lobnik, Otto S. Wolfbeis
    Abstract:

    A new luminescent europium probe is presented for the determination of phosphate (P) in Microtiter Plate format. The Assay is based on the quenching of the luminescence of the europium–tetracycline (EuTc) 1:1 complex by phosphate using a reagent concentration of 20.8 μmol/L. The probe is excited at 400 nm and displays a large Stokes’ shift of 210 nm. The emission maximum is located at 616 nm. The system works best at neutral pH 7 and is therefore suitable for phosphate determination in biological and biochemical systems. The linear range of the calibration plot is from 5 × 10−6 mol/L to 7.5 × 10−4 mol/L of phosphate, and the limit of detection is 3 μmol/L.