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Lixi Yue - One of the best experts on this subject based on the ideXlab platform.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    Summary A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 °C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 °C.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 degrees C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 degrees C.

Xianghong Wang - One of the best experts on this subject based on the ideXlab platform.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    Summary A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 °C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 °C.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 degrees C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 degrees C.

Zhenming Chi - One of the best experts on this subject based on the ideXlab platform.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    Summary A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 °C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 °C.

  • a marine killer yeast against the pathogenic yeast strain in crab portunus trituberculatus and an optimization of the toxin production
    Microbiological Research, 2007
    Co-Authors: Xianghong Wang, Zhenming Chi, Lixi Yue
    Abstract:

    A pathogenic yeast strain WCY which could cause Milky Disease in Portunus trituberculatus was identified to be Metschnikowia bicuspidate according to the results of routine yeast identification and 18S rDNA and ITS sequences. After screening of more than 300 yeast strains from different sources in marine environments, it was found that strain YF07b had the highest ability to produce killer toxin against the pathogenic yeast. Strain YF07b was identified to be Pichia anomala according to the results of routine yeast identification and 18S rDNA and ITS sequences. The optimal conditions for killer toxin production by strain YF07b were the production medium with 2.0% NaCl, pH 4.5, cultivation temperature of 20 degrees C and the optimal conditions for action of the crude killer toxin against the pathogenic yeast were the assay medium with 6.0% NaCl, pH 4.5 and temperature 15 degrees C.

Ketut Mahardika - One of the best experts on this subject based on the ideXlab platform.

  • EXPERIMENTAL INFECTIONS OF Milky HEMOLYMPH Disease IN SPINY LOBSTER Panulirus homarus
    Indonesian Aquaculture Journal, 2018
    Co-Authors: Sudewi Sudewi, Zeny Widiastuti, Bejo Slamet, Ketut Mahardika
    Abstract:

    Milky hemolymph Disease of spiny lobster (MHD-SL) is categorized as the most destructive Disease in farming spiny lobster. Therefore, it is required to investigate the routes of Milky Disease infection in spiny lobster as a basic knowledge in order to prevent Milky Disease transmission. The aim of the present study was to perform an experimental infection of Milky Disease in spiny lobster Panulirus homarus. Experimental infection of Milky Disease was carried out by several modes of infection which were injection, immersion and per os exposure. Injection of each 0.2 mL undiluted and diluted hemolymph from the Diseased lobster resulted in a cumulative mortality of 100% at 15 days post-infection (dpi), and 75% at 16 dpi, respectively. Experimental infection through water immersion caused in a cumulative mortality of 50% at 7 dpi. In contrast, no mortality was observed in per os exposure as well as in control groups. Results of this experimental study provided evidence for horizontal transmission of MHD-SL among P. homarus. Histopathological analysis exhibited that there were masses of Rickettsia-like bacteria (RLB) in the connective tissues of the gill, hepatopancreas, gonad, midgut, and muscle tissues of the affected lobsters. Mass of RLB was not only found in the moribund lobsters but also in the surviving lobsters with Milky hemolymph appearance.

  • INVESTIGATION OF DiseaseS IN GROW-OUT OF SPINY LOBSTER Panulirus homarus CULTURED IN FLOATING NET CAGES (LOMBOK, PEGAMETAN AND PANGANDARAN)
    Bogor Agricultural University, 2018
    Co-Authors: Sudewi Sudewi, Zeny Widiastuti, Bejo Slamet, Ketut Mahardika
    Abstract:

    Spiny lobster, Panulirus homarus is an economically important fishery product which indicated by huge demand of this species at both local and international markets. However, high mortalities were recorded during grow-out period due to infection of Diseases. This study was conducted to investigate Disease occurrences in grow-out of P. homarus cultured in floating net cages. The study was done throughout collection of samples, observation of parasites, isolation of fungi and bacteria, and detection of Milky Hemolymph Disease of Spiny Lobster (MHD-SL) by PCR. The samples were obtained from Lombok, Pangandaran, and Pegametan (Bali), five lobsters each. Results showed that three lobsters from Pangandaran were infected with ectoparasite Octolasmis sp. that infect mostly in the gill lamellae. One sample from Lombok was found to be infected with Fusarium sp., the causative agent of black gill Disease which indicated by black coloration of the gill. Detection of Milky Disease showed that one lobster from Pegametan and two lobsters from Lombok were infected with the Disease. Milky Disease is the most devastating Disease which causing in high mortalities within 5 days of the onset of symptoms of Milky Disease infection. Considering that MHD-SL can be transmitted rapidly, it is suggested to perform biosecurity and quarantine procedures immediately to prevent Disease translocation.  Keywords: lobster Diseases, Panulirus homarus, Milky Hemolymph Disease of Spiny Lobster (MHD-SL), black gill Disease, Octolasmis sp. 

Sudewi Sudewi - One of the best experts on this subject based on the ideXlab platform.

  • EXPERIMENTAL INFECTIONS OF Milky HEMOLYMPH Disease IN SPINY LOBSTER Panulirus homarus
    Indonesian Aquaculture Journal, 2018
    Co-Authors: Sudewi Sudewi, Zeny Widiastuti, Bejo Slamet, Ketut Mahardika
    Abstract:

    Milky hemolymph Disease of spiny lobster (MHD-SL) is categorized as the most destructive Disease in farming spiny lobster. Therefore, it is required to investigate the routes of Milky Disease infection in spiny lobster as a basic knowledge in order to prevent Milky Disease transmission. The aim of the present study was to perform an experimental infection of Milky Disease in spiny lobster Panulirus homarus. Experimental infection of Milky Disease was carried out by several modes of infection which were injection, immersion and per os exposure. Injection of each 0.2 mL undiluted and diluted hemolymph from the Diseased lobster resulted in a cumulative mortality of 100% at 15 days post-infection (dpi), and 75% at 16 dpi, respectively. Experimental infection through water immersion caused in a cumulative mortality of 50% at 7 dpi. In contrast, no mortality was observed in per os exposure as well as in control groups. Results of this experimental study provided evidence for horizontal transmission of MHD-SL among P. homarus. Histopathological analysis exhibited that there were masses of Rickettsia-like bacteria (RLB) in the connective tissues of the gill, hepatopancreas, gonad, midgut, and muscle tissues of the affected lobsters. Mass of RLB was not only found in the moribund lobsters but also in the surviving lobsters with Milky hemolymph appearance.

  • INVESTIGATION OF DiseaseS IN GROW-OUT OF SPINY LOBSTER Panulirus homarus CULTURED IN FLOATING NET CAGES (LOMBOK, PEGAMETAN AND PANGANDARAN)
    Bogor Agricultural University, 2018
    Co-Authors: Sudewi Sudewi, Zeny Widiastuti, Bejo Slamet, Ketut Mahardika
    Abstract:

    Spiny lobster, Panulirus homarus is an economically important fishery product which indicated by huge demand of this species at both local and international markets. However, high mortalities were recorded during grow-out period due to infection of Diseases. This study was conducted to investigate Disease occurrences in grow-out of P. homarus cultured in floating net cages. The study was done throughout collection of samples, observation of parasites, isolation of fungi and bacteria, and detection of Milky Hemolymph Disease of Spiny Lobster (MHD-SL) by PCR. The samples were obtained from Lombok, Pangandaran, and Pegametan (Bali), five lobsters each. Results showed that three lobsters from Pangandaran were infected with ectoparasite Octolasmis sp. that infect mostly in the gill lamellae. One sample from Lombok was found to be infected with Fusarium sp., the causative agent of black gill Disease which indicated by black coloration of the gill. Detection of Milky Disease showed that one lobster from Pegametan and two lobsters from Lombok were infected with the Disease. Milky Disease is the most devastating Disease which causing in high mortalities within 5 days of the onset of symptoms of Milky Disease infection. Considering that MHD-SL can be transmitted rapidly, it is suggested to perform biosecurity and quarantine procedures immediately to prevent Disease translocation.  Keywords: lobster Diseases, Panulirus homarus, Milky Hemolymph Disease of Spiny Lobster (MHD-SL), black gill Disease, Octolasmis sp.