The Experts below are selected from a list of 5160 Experts worldwide ranked by ideXlab platform

David J Mooney - One of the best experts on this subject based on the ideXlab platform.

  • performance and biocompatibility of extremely tough alginate polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, David J Mooney
    Abstract:

    Abstract Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ∼9000 J/m 2 , we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

  • Performance and biocompatibility of extremely tough alginate/polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, Jeong-yun Sun, Zhigang Suo, David J Mooney
    Abstract:

    Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ≈ 9000 J/m(2), we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

Praveen R Arany - One of the best experts on this subject based on the ideXlab platform.

  • performance and biocompatibility of extremely tough alginate polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, David J Mooney
    Abstract:

    Abstract Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ∼9000 J/m 2 , we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

  • Performance and biocompatibility of extremely tough alginate/polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, Jeong-yun Sun, Zhigang Suo, David J Mooney
    Abstract:

    Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ≈ 9000 J/m(2), we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

Reginald Birngruber - One of the best experts on this subject based on the ideXlab platform.

  • Histology of retinal lesions after continuous irradiation and selective micro-coagulation of the retinal pigment epithelium
    Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft, 1993
    Co-Authors: Johann Roider, Thomas J Flotte, Norman Michaud, Reginald Birngruber
    Abstract:

    Abstract Mild continuous wave (CW) irradiation (100 ms, 20 mW, 514 nm) and irradiation with 100 repetitive 5 microseconds laser pulses (3 or 6 microJ, 514 nm) at a repetition rate of 500 Hz was performed to the regio macularis of chinchilla rabbits. The angiographically visible lesions were histologically followed up to 4 weeks. With both irradiation modalities the original retinal pigment epithelium (RPE) was replaced by a monolayer of new RPE cells. Only Minimal immediate and no subsequent damage to the photoreceptors was found after selective RPE photocoagulation. Only Minimal Inflammatory Response was found after selective RPE photocoagulation in contrast to CW photocoagulation where macrophages, RPE cells and lymphocytes regularly appear in the damaged photoreceptor layer.

  • Response of the Retinal Pigment Epithelium to Selective Photocoagulation
    Archives of Ophthalmology, 1992
    Co-Authors: Johann Roider, Norm Michaud, Thomas J Flotte, Reginald Birngruber
    Abstract:

    • Multiple short argon laser pulses can coagulate the retinal pigment epithelium selectively, while sparing the adjacent neural retina and choroid; in contrast, continuous-wave laser irradiation typically damages the neural retina and choroid. The healing Response to selective photocoagulation of the retinal pigment epithelium was studied in rabbits during a period of 4 weeks. The lesions were never visible ophthalmoscopically. During the healing period, the epithelium was reformed by a single sheet of hypertrophic retinal pigment epithelial cells. In contrast to continuous-wave photocoagulation, only Minimal Inflammatory Response was found. Retinal pigment epithelial cells showed clear signs of viability, eg, phagocytized outer segments. The local edema in the photoreceptor layer and subretinal space found in the early stage disappeared when the blood-retinal barrier was reestablished. The choriocapillaris remained unaffected. No subsequent damage to the photoreceptors was found. This type of photocoagulation may be useful for retinal pigment epithelium—related diseases, eg, diffuse diabetic macular edema.

  • Response of the retinal pigment epithelium to selective photocoagulation
    1992
    Co-Authors: Johann Roider, Norm Michaud, Thomas J Flotte, Reginald Birngruber
    Abstract:

    Multiple short argon laser pulses can coagulate the retinal pigment epithelium selectively, while sparing the adjacent neural retina and choroid; in contrast, continuous-wave laser irradiation typically damages the neural retina and choroid. The healing Response to selective photocoagulation of the retinal pigment epithelium was studied in rabbits during a period of 4 weeks. The lesions were never visible ophthalmoscopically. During the healing period, the epithelium was reformed by a single sheet of hypertrophic retinal pigment epithelial cells. In contrast to continuous-wave photocoagulation, only Minimal Inflammatory Response was found

Manav Mehta - One of the best experts on this subject based on the ideXlab platform.

  • performance and biocompatibility of extremely tough alginate polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, David J Mooney
    Abstract:

    Abstract Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ∼9000 J/m 2 , we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

  • Performance and biocompatibility of extremely tough alginate/polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, Jeong-yun Sun, Zhigang Suo, David J Mooney
    Abstract:

    Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ≈ 9000 J/m(2), we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

Max Darnell - One of the best experts on this subject based on the ideXlab platform.

  • performance and biocompatibility of extremely tough alginate polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, David J Mooney
    Abstract:

    Abstract Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ∼9000 J/m 2 , we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.

  • Performance and biocompatibility of extremely tough alginate/polyacrylamide hydrogels
    Biomaterials, 2013
    Co-Authors: Max Darnell, Manav Mehta, Christopher Johnson, Praveen R Arany, Jeong-yun Sun, Zhigang Suo, David J Mooney
    Abstract:

    Although hydrogels now see widespread use in a host of applications, low fracture toughness and brittleness have limited their more broad use. As a recently described interpenetrating network (IPN) of alginate and polyacrylamide demonstrated a fracture toughness of ≈ 9000 J/m(2), we sought to explore the biocompatibility and maintenance of mechanical properties of these hydrogels in cell culture and in vivo conditions. These hydrogels can sustain a compressive strain of over 90% with Minimal loss of Young's Modulus as well as Minimal swelling for up to 50 days of soaking in culture conditions. Mouse mesenchymal stem cells exposed to the IPN gel-conditioned media maintain high viability, and although cells exposed to conditioned media demonstrate slight reductions in proliferation and metabolic activity (WST assay), these effects are abrogated in a dose-dependent manner. Implantation of these IPN hydrogels into subcutaneous tissue of rats for 8 weeks led to mild fibrotic encapsulation and Minimal Inflammatory Response. These results suggest the further exploration of extremely tough alginate/PAAM IPN hydrogels as biomaterials.