The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Steven Russell - One of the best experts on this subject based on the ideXlab platform.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Development of a CRISPR/Cas9-based gene drive system in Anopheles gambiae, the main vector for the malaria parasite, paves the way for control of this pest insect. Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
Andrew Hammond - One of the best experts on this subject based on the ideXlab platform.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Development of a CRISPR/Cas9-based gene drive system in Anopheles gambiae, the main vector for the malaria parasite, paves the way for control of this pest insect. Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
J Paya - One of the best experts on this subject based on the ideXlab platform.
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technical reportphysical and mechanical properties of foamed portland cement composite containing crumb rubber from worn tires
Materials & Design, 2014Co-Authors: J N Eiras, F Segovia, M V Borrachero, J Monzo, M Bonilla, J PayaAbstract:The management of worn tires is a concern in industrialized countries. The application of crumb rubber as lightweight aggregate in cement based materials is a green alternative for reusing this material. High replacements of natural sand by crumb rubber were studied and an air-entraining agent was employed to ensure a cellular structure in the cement-based composite. The obtained results from tests in fresh state reveal an improvement in workability. The tests conducted on hardened composite show promise for constructive applications where thermal and acoustic properties are required. The Minimum Requirement of mechanical strength for masonry units was achieved, since compressive strengths varied between 1 and 10 MPa. Finally, potential applications as a construction material have been highlighted.
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physical and mechanical properties of foamed portland cement composite containing crumb rubber from worn tires
Materials & Design, 2014Co-Authors: J N Eiras, F Segovia, M V Borrachero, J Monzo, M Bonilla, J PayaAbstract:Abstract The management of worn tires is a concern in industrialized countries. The application of crumb rubber as lightweight aggregate in cement based materials is a green alternative for reusing this material. High replacements of natural sand by crumb rubber were studied and an air-entraining agent was employed to ensure a cellular structure in the cement-based composite. The obtained results from tests in fresh state reveal an improvement in workability. The tests conducted on hardened composite show promise for constructive applications where thermal and acoustic properties are required. The Minimum Requirement of mechanical strength for masonry units was achieved, since compressive strengths varied between 1 and 10 MPa. Finally, potential applications as a construction material have been highlighted.
Eric Marois - One of the best experts on this subject based on the ideXlab platform.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Development of a CRISPR/Cas9-based gene drive system in Anopheles gambiae, the main vector for the malaria parasite, paves the way for control of this pest insect. Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
Roberto Galizi - One of the best experts on this subject based on the ideXlab platform.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Development of a CRISPR/Cas9-based gene drive system in Anopheles gambiae, the main vector for the malaria parasite, paves the way for control of this pest insect. Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
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a crispr cas9 gene drive system targeting female reproduction in the malaria mosquito vector anopheles gambiae
Nature Biotechnology, 2016Co-Authors: Andrew Hammond, Roberto Galizi, Kyros Kyrou, Alekos Simoni, Carla Siniscalchi, Dimitris Katsanos, Matthew Gribble, Dean A Baker, Eric Marois, Steven RussellAbstract:Gene drive systems that enable super-Mendelian inheritance of a transgene have the potential to modify insect populations over a timeframe of a few years. We describe CRISPR-Cas9 endonuclease constructs that function as gene drive systems in Anopheles gambiae, the main vector for malaria. We identified three genes (AGAP005958, AGAP011377 and AGAP007280) that confer a recessive female-sterility phenotype upon disruption, and inserted into each locus CRISPR-Cas9 gene drive constructs designed to target and edit each gene. For each targeted locus we observed a strong gene drive at the molecular level, with transmission rates to progeny of 91.4 to 99.6%. Population modeling and cage experiments indicate that a CRISPR-Cas9 construct targeting one of these loci, AGAP007280, meets the Minimum Requirement for a gene drive targeting female reproduction in an insect population. These findings could expedite the development of gene drives to suppress mosquito populations to levels that do not support malaria transmission.
