The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
Stephen D. Cederbaum - One of the best experts on this subject based on the ideXlab platform.
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Subcellular location and differential Antibody specificity of arginase in tissue culture and whole animals
International Journal of Developmental Neuroscience, 1994Co-Authors: E B Spector, Christopher P. Jenkinson, Murray R. Grigor, Rita M. Kern, Stephen D. CederbaumAbstract:Abstract Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the Mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An Antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
E B Spector - One of the best experts on this subject based on the ideXlab platform.
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Subcellular location and differential Antibody specificity of arginase in tissue culture and whole animals
International Journal of Developmental Neuroscience, 1994Co-Authors: E B Spector, Christopher P. Jenkinson, Murray R. Grigor, Rita M. Kern, Stephen D. CederbaumAbstract:Abstract Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the Mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An Antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
V. Eusebi - One of the best experts on this subject based on the ideXlab platform.
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GANT-like gastrointestinal pacemaker cell tumours with oncocytic features
Virchows Archiv, 1999Co-Authors: Stefania Damiani, Gianandrea Pasquinelli, V. EusebiAbstract:We describe two cases of gastrointestinal stromal tumours with prominent oncocytic features. Both had features consistent with differentiation towards the interstitial cells of Cajal (CC). They were composed of nests and bundles of cells with abundant, deeply granular, eosinophilic cytoplasm. Immunohistochemical investigations revealed positivity with c- kit , vimentin and CD34 antibodies in both neoplasms. Ultrastructurally the neoplastic cells showed characteristic features of CC; they had synapse-like structures and dense core cytoplasmic granules. Oncocytic features were confirmed by immunohistochemistry using anti-Mitochondrion Antibody in both cases and by electron microscopy in one case (case 1). Although the CC are frequently described as Mitochondrion-rich cells, oncocytic changes have not previously been reported as a feature of gastrointestinal autonomic nerve tumour (GANT)-like stromal tumours.
Christopher P. Jenkinson - One of the best experts on this subject based on the ideXlab platform.
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Subcellular location and differential Antibody specificity of arginase in tissue culture and whole animals
International Journal of Developmental Neuroscience, 1994Co-Authors: E B Spector, Christopher P. Jenkinson, Murray R. Grigor, Rita M. Kern, Stephen D. CederbaumAbstract:Abstract Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the Mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An Antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.
Murray R. Grigor - One of the best experts on this subject based on the ideXlab platform.
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Subcellular location and differential Antibody specificity of arginase in tissue culture and whole animals
International Journal of Developmental Neuroscience, 1994Co-Authors: E B Spector, Christopher P. Jenkinson, Murray R. Grigor, Rita M. Kern, Stephen D. CederbaumAbstract:Abstract Studies in man and other mammals have demonstrated the existence of two forms of arginase, a cytoplasmic form located primarily in liver and a mitochondrial form expressed in lesser amounts in a larger number of organs, but especially kidney. They appear to be encoded in different gene loci. Using a colloidal silica gradient separation technique, we have now located arginase in H4 cells, a rat hepatoma-derived line, to the cytoplasm and the arginase in human embryonic kidney-derived line, to the Mitochondrion. Antibody prepared against A1 precipitates all the arginase from liver, 50% from kidney and none of the activity from human embryonic kidney (HEK) cells. An Antibody prepared against partially purified All, by contrast, precipitates >90% of arginase activity from HEK cells, half from kidney and virtually none from H4 cells or rat liver.