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Jacques Bejuihugues - One of the best experts on this subject based on the ideXlab platform.
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is isolated Mobile Component exchange an option in the management of intraprosthetic dislocation of a dual mobility cup
Clinical Orthopaedics and Related Research, 2020Co-Authors: Julien Wegrzyn, Matthieu Malatray, Vincent Pibarot, Gaetano Anania, Jacques BejuihuguesAbstract:BACKGROUND Intraprosthetic dislocation is a specific complication of dual mobility cups, although it occurs less frequently with the latest generations of implants. Intraprosthetic dislocation is related to long-term polyethylene wear of the Mobile Component chamfer and retentive area, leading to a snap-out of the femoral head. With the increased use of dual mobility cups, even in younger and active patients, the management of intraprosthetic dislocation should be defined according to its type. However, no previous studies, except for case reports, have described the strategy to manage long-term wear-related intraprosthetic dislocation, particularly when a dual mobility cup is not loose. QUESTIONS/PURPOSES This study aimed to (1) determine the prevalence of intraprosthetic dislocation in this patient population and the macroscopic findings at the time of surgical revision and (2) evaluate whether isolated Mobile Component exchange could be an option to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. METHODS From January 1991 to December 2009, a continuous series of 5274 THAs with dual mobility cups (4546 patients; 2773 women; mean [range] age 58 years [22-87]; bilateral THA = 728) were prospectively enrolled in our institutional total joint registry. A cementless, hemispherical dual mobility cup was systematically implanted, regardless of the patient's age or indication for THA. At the latest follow-up examination, the registry was queried to isolate each occurrence of intraprosthetic dislocation, which was retrospectively analyzed regarding the patient's demographics, indication for THA, radiographs, intraoperative findings (polyethylene wear and lesion patterns on the Mobile Component, periarticular metallosis, and implant damage because of intraprosthetic impingement of the femoral neck), management of intraprosthetic dislocation (isolated exchange of the Mobile Component or revision of the dual mobility cup), and outcome. RESULTS At a mean (range) follow-up duration of 14 years (3-26), 3% of intraprosthetic dislocations (169 of 5274) were reported, with a mean (range) time from THA of 18 years (13-22). Intraprosthetic dislocation occurred predominantly in younger men (mean [range] age at THA, 42 years [22-64] versus 61 years [46-87]; p < 0.001, and sex ratio (male to female, 1:32 [96 male and 73 female] versus 0.62 [1677 male and 2700 female]; p < 0.001) in patients with intraprosthetic dislocation and those without, respectively, but was not influenced by the indication for THA (105 patients with intraprosthetic dislocation who underwent THA for primary hip osteoarthritis and 64 with other diagnoses versus 3146 patients without who underwent THA for primary hip osteoarthritis and 1959 for other diagnoses (p = 0.9)). In all patients with intraprosthetic dislocation, a macroscopic analysis of the explanted Mobile Component revealed circumferential polyethylene wear and damage to the chamfer and retentive area, with subsequent loss of retaining power for the femoral head. Nine percent of intraprosthetic dislocations (16 of 169 patients with intraprosthetic dislocations) were associated with aseptic loosening of the dual mobility cup and were managed with acetabular revision without recurrence at a mean (range) follow-up duration of 7.5 years (5-11). Ninety-one percent of intraprosthetic dislocations (153 of 169) were pure, related to wear of the Mobile Component chamfer and retentive area without aseptic loosening of the dual mobility cup, and managed with isolated Mobile Component exchange. Intraprosthetic dislocation recurred in 6% (nine of 153) at a mean (range) follow-up interval of 3 years (2-4.5). Additionally, severe premature polyethylene wear of the Mobile Component with loosening of the dual mobility cup occurred in 12% of patients (19 of 153) at a mean (range) follow-up duration of 1.5 years (0.5-3). CONCLUSIONS A failure rate of 18% (28 of 153 patients undergoing isolated Mobile Component exchange) was reported within 5 years after isolated Mobile Component exchange to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. The two modes of failure were early recurrence of intraprosthetic dislocation or severe premature metallosis-related polyethylene wear of the Mobile Component with loosening of the dual mobility cup. Acetabular revision with synovectomy should remain the standard procedure to manage intraprosthetic dislocation, particularly if periarticular metallosis is present. The exception is intraprosthetic dislocation occurring in elderly or frail patients, for whom a conventional acetabular revision procedure would be associated with an unjustified surgical or anesthetic risk. LEVEL OF EVIDENCE Level II, prognostic study.
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can dual mobility cups prevent dislocation in all situations after revision total hip arthroplasty
Journal of Arthroplasty, 2015Co-Authors: Julien Wegrzyn, Jacques Bejuihugues, Eloise Tebaa, Alexandre Jacquel, J P Carret, Vincent PibarotAbstract:Abstract The outcome of a single design of dual mobility cup was prospectively evaluated in a continuous series of 994 revision THAs with respect to dislocation and intra-prosthetic dislocation (IPD). At a 7.3-year mean follow-up, the dislocation rate was 1.5% and the IPD rate was 0.2%. The 2 IPD occurred in acetabular-only revisions and were related to a poor head-to-neck ratio with early impingement and wear at the polyethylene Mobile Component chamfer. Dual mobility cups demonstrated a low dislocation rate in revision THA but did not compensate for potential perioperative technical errors. In addition, IPD did not appear to be a concern with respect to the benefit in term of instability prevention though caution is advised in acetabular-only revision associated with a poor head-to-neck ratio. Level of Evidence: Therapeutic study—Level IV
Julien Wegrzyn - One of the best experts on this subject based on the ideXlab platform.
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is isolated Mobile Component exchange an option in the management of intraprosthetic dislocation of a dual mobility cup
Clinical Orthopaedics and Related Research, 2020Co-Authors: Julien Wegrzyn, Matthieu Malatray, Vincent Pibarot, Gaetano Anania, Jacques BejuihuguesAbstract:BACKGROUND Intraprosthetic dislocation is a specific complication of dual mobility cups, although it occurs less frequently with the latest generations of implants. Intraprosthetic dislocation is related to long-term polyethylene wear of the Mobile Component chamfer and retentive area, leading to a snap-out of the femoral head. With the increased use of dual mobility cups, even in younger and active patients, the management of intraprosthetic dislocation should be defined according to its type. However, no previous studies, except for case reports, have described the strategy to manage long-term wear-related intraprosthetic dislocation, particularly when a dual mobility cup is not loose. QUESTIONS/PURPOSES This study aimed to (1) determine the prevalence of intraprosthetic dislocation in this patient population and the macroscopic findings at the time of surgical revision and (2) evaluate whether isolated Mobile Component exchange could be an option to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. METHODS From January 1991 to December 2009, a continuous series of 5274 THAs with dual mobility cups (4546 patients; 2773 women; mean [range] age 58 years [22-87]; bilateral THA = 728) were prospectively enrolled in our institutional total joint registry. A cementless, hemispherical dual mobility cup was systematically implanted, regardless of the patient's age or indication for THA. At the latest follow-up examination, the registry was queried to isolate each occurrence of intraprosthetic dislocation, which was retrospectively analyzed regarding the patient's demographics, indication for THA, radiographs, intraoperative findings (polyethylene wear and lesion patterns on the Mobile Component, periarticular metallosis, and implant damage because of intraprosthetic impingement of the femoral neck), management of intraprosthetic dislocation (isolated exchange of the Mobile Component or revision of the dual mobility cup), and outcome. RESULTS At a mean (range) follow-up duration of 14 years (3-26), 3% of intraprosthetic dislocations (169 of 5274) were reported, with a mean (range) time from THA of 18 years (13-22). Intraprosthetic dislocation occurred predominantly in younger men (mean [range] age at THA, 42 years [22-64] versus 61 years [46-87]; p < 0.001, and sex ratio (male to female, 1:32 [96 male and 73 female] versus 0.62 [1677 male and 2700 female]; p < 0.001) in patients with intraprosthetic dislocation and those without, respectively, but was not influenced by the indication for THA (105 patients with intraprosthetic dislocation who underwent THA for primary hip osteoarthritis and 64 with other diagnoses versus 3146 patients without who underwent THA for primary hip osteoarthritis and 1959 for other diagnoses (p = 0.9)). In all patients with intraprosthetic dislocation, a macroscopic analysis of the explanted Mobile Component revealed circumferential polyethylene wear and damage to the chamfer and retentive area, with subsequent loss of retaining power for the femoral head. Nine percent of intraprosthetic dislocations (16 of 169 patients with intraprosthetic dislocations) were associated with aseptic loosening of the dual mobility cup and were managed with acetabular revision without recurrence at a mean (range) follow-up duration of 7.5 years (5-11). Ninety-one percent of intraprosthetic dislocations (153 of 169) were pure, related to wear of the Mobile Component chamfer and retentive area without aseptic loosening of the dual mobility cup, and managed with isolated Mobile Component exchange. Intraprosthetic dislocation recurred in 6% (nine of 153) at a mean (range) follow-up interval of 3 years (2-4.5). Additionally, severe premature polyethylene wear of the Mobile Component with loosening of the dual mobility cup occurred in 12% of patients (19 of 153) at a mean (range) follow-up duration of 1.5 years (0.5-3). CONCLUSIONS A failure rate of 18% (28 of 153 patients undergoing isolated Mobile Component exchange) was reported within 5 years after isolated Mobile Component exchange to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. The two modes of failure were early recurrence of intraprosthetic dislocation or severe premature metallosis-related polyethylene wear of the Mobile Component with loosening of the dual mobility cup. Acetabular revision with synovectomy should remain the standard procedure to manage intraprosthetic dislocation, particularly if periarticular metallosis is present. The exception is intraprosthetic dislocation occurring in elderly or frail patients, for whom a conventional acetabular revision procedure would be associated with an unjustified surgical or anesthetic risk. LEVEL OF EVIDENCE Level II, prognostic study.
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can dual mobility cups prevent dislocation in all situations after revision total hip arthroplasty
Journal of Arthroplasty, 2015Co-Authors: Julien Wegrzyn, Jacques Bejuihugues, Eloise Tebaa, Alexandre Jacquel, J P Carret, Vincent PibarotAbstract:Abstract The outcome of a single design of dual mobility cup was prospectively evaluated in a continuous series of 994 revision THAs with respect to dislocation and intra-prosthetic dislocation (IPD). At a 7.3-year mean follow-up, the dislocation rate was 1.5% and the IPD rate was 0.2%. The 2 IPD occurred in acetabular-only revisions and were related to a poor head-to-neck ratio with early impingement and wear at the polyethylene Mobile Component chamfer. Dual mobility cups demonstrated a low dislocation rate in revision THA but did not compensate for potential perioperative technical errors. In addition, IPD did not appear to be a concern with respect to the benefit in term of instability prevention though caution is advised in acetabular-only revision associated with a poor head-to-neck ratio. Level of Evidence: Therapeutic study—Level IV
Vincent Pibarot - One of the best experts on this subject based on the ideXlab platform.
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is isolated Mobile Component exchange an option in the management of intraprosthetic dislocation of a dual mobility cup
Clinical Orthopaedics and Related Research, 2020Co-Authors: Julien Wegrzyn, Matthieu Malatray, Vincent Pibarot, Gaetano Anania, Jacques BejuihuguesAbstract:BACKGROUND Intraprosthetic dislocation is a specific complication of dual mobility cups, although it occurs less frequently with the latest generations of implants. Intraprosthetic dislocation is related to long-term polyethylene wear of the Mobile Component chamfer and retentive area, leading to a snap-out of the femoral head. With the increased use of dual mobility cups, even in younger and active patients, the management of intraprosthetic dislocation should be defined according to its type. However, no previous studies, except for case reports, have described the strategy to manage long-term wear-related intraprosthetic dislocation, particularly when a dual mobility cup is not loose. QUESTIONS/PURPOSES This study aimed to (1) determine the prevalence of intraprosthetic dislocation in this patient population and the macroscopic findings at the time of surgical revision and (2) evaluate whether isolated Mobile Component exchange could be an option to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. METHODS From January 1991 to December 2009, a continuous series of 5274 THAs with dual mobility cups (4546 patients; 2773 women; mean [range] age 58 years [22-87]; bilateral THA = 728) were prospectively enrolled in our institutional total joint registry. A cementless, hemispherical dual mobility cup was systematically implanted, regardless of the patient's age or indication for THA. At the latest follow-up examination, the registry was queried to isolate each occurrence of intraprosthetic dislocation, which was retrospectively analyzed regarding the patient's demographics, indication for THA, radiographs, intraoperative findings (polyethylene wear and lesion patterns on the Mobile Component, periarticular metallosis, and implant damage because of intraprosthetic impingement of the femoral neck), management of intraprosthetic dislocation (isolated exchange of the Mobile Component or revision of the dual mobility cup), and outcome. RESULTS At a mean (range) follow-up duration of 14 years (3-26), 3% of intraprosthetic dislocations (169 of 5274) were reported, with a mean (range) time from THA of 18 years (13-22). Intraprosthetic dislocation occurred predominantly in younger men (mean [range] age at THA, 42 years [22-64] versus 61 years [46-87]; p < 0.001, and sex ratio (male to female, 1:32 [96 male and 73 female] versus 0.62 [1677 male and 2700 female]; p < 0.001) in patients with intraprosthetic dislocation and those without, respectively, but was not influenced by the indication for THA (105 patients with intraprosthetic dislocation who underwent THA for primary hip osteoarthritis and 64 with other diagnoses versus 3146 patients without who underwent THA for primary hip osteoarthritis and 1959 for other diagnoses (p = 0.9)). In all patients with intraprosthetic dislocation, a macroscopic analysis of the explanted Mobile Component revealed circumferential polyethylene wear and damage to the chamfer and retentive area, with subsequent loss of retaining power for the femoral head. Nine percent of intraprosthetic dislocations (16 of 169 patients with intraprosthetic dislocations) were associated with aseptic loosening of the dual mobility cup and were managed with acetabular revision without recurrence at a mean (range) follow-up duration of 7.5 years (5-11). Ninety-one percent of intraprosthetic dislocations (153 of 169) were pure, related to wear of the Mobile Component chamfer and retentive area without aseptic loosening of the dual mobility cup, and managed with isolated Mobile Component exchange. Intraprosthetic dislocation recurred in 6% (nine of 153) at a mean (range) follow-up interval of 3 years (2-4.5). Additionally, severe premature polyethylene wear of the Mobile Component with loosening of the dual mobility cup occurred in 12% of patients (19 of 153) at a mean (range) follow-up duration of 1.5 years (0.5-3). CONCLUSIONS A failure rate of 18% (28 of 153 patients undergoing isolated Mobile Component exchange) was reported within 5 years after isolated Mobile Component exchange to manage intraprosthetic dislocation occurring with a well-fixed dual mobility cup metal shell. The two modes of failure were early recurrence of intraprosthetic dislocation or severe premature metallosis-related polyethylene wear of the Mobile Component with loosening of the dual mobility cup. Acetabular revision with synovectomy should remain the standard procedure to manage intraprosthetic dislocation, particularly if periarticular metallosis is present. The exception is intraprosthetic dislocation occurring in elderly or frail patients, for whom a conventional acetabular revision procedure would be associated with an unjustified surgical or anesthetic risk. LEVEL OF EVIDENCE Level II, prognostic study.
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can dual mobility cups prevent dislocation in all situations after revision total hip arthroplasty
Journal of Arthroplasty, 2015Co-Authors: Julien Wegrzyn, Jacques Bejuihugues, Eloise Tebaa, Alexandre Jacquel, J P Carret, Vincent PibarotAbstract:Abstract The outcome of a single design of dual mobility cup was prospectively evaluated in a continuous series of 994 revision THAs with respect to dislocation and intra-prosthetic dislocation (IPD). At a 7.3-year mean follow-up, the dislocation rate was 1.5% and the IPD rate was 0.2%. The 2 IPD occurred in acetabular-only revisions and were related to a poor head-to-neck ratio with early impingement and wear at the polyethylene Mobile Component chamfer. Dual mobility cups demonstrated a low dislocation rate in revision THA but did not compensate for potential perioperative technical errors. In addition, IPD did not appear to be a concern with respect to the benefit in term of instability prevention though caution is advised in acetabular-only revision associated with a poor head-to-neck ratio. Level of Evidence: Therapeutic study—Level IV
Jurgen Janek - One of the best experts on this subject based on the ideXlab platform.
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guidelines for all solid state battery design and electrode buffer layers based on chemical potential profile calculation
ACS Applied Materials & Interfaces, 2019Co-Authors: Takashi Nakamura, Koji Amezawa, Jörn Kulisch, Wolfgang G. Zeier, Jurgen JanekAbstract:Protective coatings on cathode active materials have become paramount for the implementation of solid-state batteries; however, the development of coatings lacks the understanding of the necessary coating properties. In this study, guidelines for the design of solid electrolytes and electrode coatings in all-solid-state batteries are proposed from the viewpoint of the steady-state Li chemical potential profile across the battery cell. The model calculation of the (electro)chemical potential profile in all-solid-state batteries is established by considering the steady-state mixed ionic and electronic conduction in the solid electrolyte under the assumption of local equilibrium. For quantitative discussion, the potential profiles within oxygen ion conductors are calculated instead of Li/Na ion conductors as their partial electronic conductivities have not been reported so far in sufficient detail. Based on the calculated chemical potential profile, two main conclusions are obtained: (1) the decisive factor for the formation of the chemical potential profile of the neutral Mobile Component (e.g., oxygen or lithium) in the solid electrolyte is its electronic conductivity (and the activity dependence), and (2) a particularly large potential drop is formed in a region where the electronic conductivity becomes small. While these conclusions are valid and general for any solid electrolyte device, they are particularly important for the design of protective coatings and the understanding of the functionality of self-assembled solid electrolyte interphases in all-solid-state batteries. To protect the solid electrolyte from decomposition by reduction/oxidation at the anode/cathode interfaces, a sufficient chemical potential drop is necessary within the coating layer or directly at the interphase layer. To achieve this situation, the coating/interphase materials need to have a lower electronic conductivity than the solid electrolyte.
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Guidelines for All-Solid-State Battery Design and Electrode Buffer Layers Based on Chemical Potential Profile Calculation
2019Co-Authors: Takashi Nakamura, Koji Amezawa, Jörn Kulisch, Wolfgang G. Zeier, Jurgen JanekAbstract:Protective coatings on cathode active materials have become paramount for the implementation of solid-state batteries; however, the development of coatings lacks the understanding of the necessary coating properties. In this study, guidelines for the design of solid electrolytes and electrode coatings in all-solid-state batteries are proposed from the viewpoint of the steady-state Li chemical potential profile across the battery cell. The model calculation of the (electro)chemical potential profile in all-solid-state batteries is established by considering the steady-state mixed ionic and electronic conduction in the solid electrolyte under the assumption of local equilibrium. For quantitative discussion, the potential profiles within oxygen ion conductors are calculated instead of Li/Na ion conductors as their partial electronic conductivities have not been reported so far in sufficient detail. Based on the calculated chemical potential profile, two main conclusions are obtained: (1) the decisive factor for the formation of the chemical potential profile of the neutral Mobile Component (e.g., oxygen or lithium) in the solid electrolyte is its electronic conductivity (and the activity dependence), and (2) a particularly large potential drop is formed in a region where the electronic conductivity becomes small. While these conclusions are valid and general for any solid electrolyte device, they are particularly important for the design of protective coatings and the understanding of the functionality of self-assembled solid electrolyte interphases in all-solid-state batteries. To protect the solid electrolyte from decomposition by reduction/oxidation at the anode/cathode interfaces, a sufficient chemical potential drop is necessary within the coating layer or directly at the interphase layer. To achieve this situation, the coating/interphase materials need to have a lower electronic conductivity than the solid electrolyte
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thermomigration and soret effect in naxcoo2 as thermoelectric material preparation and characterization of sodium cobaltate thin films
Physica Status Solidi (a), 2016Co-Authors: Christian Schneider, Patrick Schichtel, Boris Mogwitz, Rainer Straubinger, Andreas Beyer, Marcus Rohnke, K Volz, Jurgen JanekAbstract:Sodium cobaltate (NaxCoO2, NCO) is a model type thermoelectric material for high temperature applications, which is representative for the class of non-stoichiometric and mixed ion-electron conducting thermoelectrics (e.g., Cu2 – xSe). The present study deals with the kinetic instability of the originally homogeneous chemical composition when a temperature gradient is applied – a common situation in thermoelectric materials with a Mobile Component (element), but rarely considered in thermoelectric materials research so far. In order to investigate a well reproducible system, highly epitaxial thin films of NaxCoO2 with an atmosphere protective capping layer of alumina are prepared via pulsed laser deposition on sapphire (001) and lanthanum aluminate (111). A self-designed non-isothermal set-up allows the precise determination of the heat of transport for Mobile sodium as 8.3 kJ mol−1 and, therefore, the quantification of thermodiffusion (i.e., the Ludwig-Soret effect in the stationary state). The experiments also allow to estimate the chemical diffusion coefficient of sodium at 422 K as about = 5 × 10−4 cm2 s−1.
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coulometric titration at low temperatures nonstoichiometric silver selenide
Solid State Ionics, 2004Co-Authors: G Beck, Jurgen JanekAbstract:Abstract A modified coulometric titration technique is described for the investigation of nonstoichiometric phases at low temperatures. It allows to obtain titration curves at temperatures where the conventional coulometric titration technique fails because of too small chemical diffusion coefficients of the Mobile Component. This method for indirect coulometric titration is applied to silver selenide between −100 and 100 °C. The titration curves are analyzed on the basis of a defect chemical model and provide thermodynamic data of the α-silver selenide phase. The boundaries of the homogeneous phase field are determined.
Deepti Mithrananda - One of the best experts on this subject based on the ideXlab platform.
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vesicular delivery of the antifungal antibiotics of lysobacter enzymogenes c3
Applied and Environmental Microbiology, 2018Co-Authors: Paul Meers, Carol Liu, Rensa Chen, William Bartos, Julianne Davis, Nicole Dziedzic, Jason Orciuolo, Szymon Kutyla, Maria Jose Pozo, Deepti MithranandaAbstract:ABSTRACT Lysobacter enzymogenes C3 is a predatory strain of Gram-negative gliding bacteria that produces antifungal antibiotics by the polyketide synthetic pathway. Outer membrane vesicles (OMV) are formed as a stress response and can deliver virulence factors to host cells. The production of OMV by C3 and their role in antifungal activity are reported here. Vesicles in the range of 130 to 150 nm in diameter were discovered in the cell-free supernatants of C3 cultures. These OMV contain molecules characteristic of bacterial outer membranes, such as lipopolysaccharide and phospholipids. In addition, they contain chitinase activity and essentially all of the heat-stable antifungal activity in cell supernatants. We show here that C3 OMV can directly inhibit growth of the yeast Saccharomyces cerevisiae as well as that of the filamentous fungus Fusarium subglutinans. The activity is dependent on physical contact between OMV and the cells. Furthermore, fluorescent lipid labeling of C3 OMV demonstrated transfer of the membrane-associated probe to yeast cells, suggesting the existence of a mechanism of delivery for membrane-associated molecules. Mass spectrometric analysis of C3 OMV extracts indicates the presence of molecules with molecular weights identical to some of the previously identified antifungal products of C3. These data together suggest that OMV act as an important remote Mobile Component of predation by Lysobacter. IMPORTANCE The data presented here suggest a newly discovered function of outer membrane vesicles (OMV) that are produced from the outer membrane of the bacterial species Lysobacter enzymogenes strain C3. We show that these OMV can be released from the surface of the cells to deliver antibiotics to target fungal organisms as a mechanism of killing or growth inhibition. Understanding the role of OMV in antibiotic delivery can generally lead to improved strategies for dealing with antibiotic-resistant organisms. These results also add to the evidence that some bacterially produced antibiotics can be discovered and purified using methods designed for isolation of nanoscale vesicles. Information on these systems can lead to better identification of active molecules or design of delivery vehicles for these molecules.
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vesicular delivery of the antifungal antibiotics of lysobacter enzymogenes c3
bioRxiv, 2018Co-Authors: Paul Meers, Carol Liu, Rensa Chen, William Bartos, Julianne Davis, Nicole Dziedzic, Jason Orciuolo, Szymon Kutyla, Maria Jose Pozo, Deepti MithranandaAbstract:Lysobacter enzymogenes C3 is a predatory strain of gram-negative gliding bacteria that produces antifungal antibiotics by the polyketide synthetic pathway. Outer membrane vesicles (OMV) are formed as a stress response and can deliver virulence factors to host cells. The production of OMV by C3 and their role in antifungal activity are reported here. Vesicles in the range of 130-150 nm in diameter were discovered in the cell-free supernatants of C3 cultures. These OMV contain molecules characteristic of bacterial outer membranes, such as lipopolysaccharide and phospholipids. In addition, they contain chitinase activity and essentially all of the heat stable antifungal activity in cell supernatants. We show here that C3 OMV can directly inhibit growth of the yeast Saccharomyces cerevisiae as well as the filamentous fungus Fusarium subglutinans. The activity is dependent on physical contact between OMV and the cells. Furthermore, fluorescent lipid labeling of C3 OMV demonstrated transfer of the membrane-associated probe to yeast cells, suggesting the existence of a mechanism of delivery for membrane-associated molecules. Mass spectrometric analysis of C3 OMV extracts indicates the presence of molecules with molecular weights identical to some of the previously identified antifungal products of C3. These data together suggest that OMV act as an important remote Mobile Component of predation by Lysobacter.