The Experts below are selected from a list of 108 Experts worldwide ranked by ideXlab platform
Kazuhide Kimbara - One of the best experts on this subject based on the ideXlab platform.
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Table_9_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.XLSX
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
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Data_Sheet_1_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.pdf
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
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Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure
Frontiers Media S.A., 2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide KimbaraAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments
Satoshi Ichiyama - One of the best experts on this subject based on the ideXlab platform.
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interspecies dissemination of a Mobilizable Plasmid harboring blaimp 19 and the possibility of horizontal gene transfer in a single patient
Antimicrobial Agents and Chemotherapy, 2016Co-Authors: Masaki Yamamoto, Yasufumi Matsumura, Ryota Gomi, Tomonari Matsuda, Michio Tanaka, Miki Nagao, Shunji Takakura, Shinji Uemoto, Satoshi IchiyamaAbstract:Carbapenemase-producing Gram-negative bacilli have been a global concern over the past 2 decades because these organisms can cause severe infections with high mortality rates. Carbapenemase genes are often carried by mobile genetic elements, and resistance Plasmids can be transferred through conjugation. We conducted whole-genome sequencing (WGS) to demonstrate that the same Plasmid harboring a metallo-β-lactamase gene was detected in two different species isolated from a single patient. Metallo-β-lactamase-producing Achromobacter xylosoxidans (KUN4507), non-metallo-β-lactamase-producing Klebsiella pneumoniae (KUN4843), and metallo-β-lactamase-producing K. pneumoniae (KUN5033) were sequentially isolated from a single patient and then analyzed in this study. Antimicrobial susceptibility testing, molecular typing (pulsed-field gel electrophoresis and multilocus sequence typing), and conjugation analyses were performed by conventional methods. Phylogenetic and molecular clock analysis of K. pneumoniae isolates were performed with WGS, and the nucleotide sequences of Plasmids detected from these isolates were determined using WGS. Conventional molecular typing revealed that KUN4843 and KUN5033 were identical, whereas the phylogenetic tree analysis revealed a slight difference. These two isolates were separated from the most recent common ancestor 0.74 years before they were isolated. The same resistance Plasmid harboring blaIMP-19 was detected in metallo-β-lactamase-producing A. xylosoxidans and K. pneumoniae Although this Plasmid was not self-transferable, the conjugation of this Plasmid from A. xylosoxidans to non-metallo-β-lactamase-producing K. pneumoniae was successfully performed. The susceptibility patterns for metallo-β-lactamase-producing K. pneumoniae and the transconjugant were similar. These findings supported the possibility of the horizontal transfer of Plasmid-borne blaIMP-19 from A. xylosoxidans to K. pneumoniae in a single patient.
Jose Berenguer - One of the best experts on this subject based on the ideXlab platform.
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two nitrate nitrite transporters are encoded within the Mobilizable Plasmid for nitrate respiration of thermus thermophilus hb8
Journal of Bacteriology, 2000Co-Authors: Sandra I Ramirez, Renata Moreno, Olga Zafra, Pablo Castan, Cristina Valles, Jose BerenguerAbstract:Thermus thermophilus HB8 can grow anaerobically by using a membrane-bound nitrate reductase to catalyze the reduction of nitrate as a final electron acceptor in respiration. In contrast to other denitrifiers, the nitrite produced does not continue the reduction pathway but accumulates in the growth medium after its active extrusion from the cell. We describe the presence of two genes, narK1 and narK2, downstream of the nitrate reductase-encoding gene cluster (nar) that code for two homologues to the major facilitator superfamily of transporters. The sequences of NarK1 and NarK2 are 30% identical to each other, but whereas NarK1 clusters in an average-distance tree with putative nitrate transporters, NarK2 does so with putative nitrite exporters. To analyze whether this differential clustering was actually related to functional differences, we isolated derivatives with mutations of one or both genes. Analysis revealed that single mutations had minor effects on growth by nitrate respiration, whereas a double narK1 narK2 mutation abolished this capability. Further analysis allowed us to confirm that the double mutant is completely unable to excrete nitrite, while single mutants have a limitation in the excretion rates compared with the wild type. These data allow us to propose that both proteins are implicated in the transport of nitrate and nitrite, probably acting as nitrate/nitrite antiporters. The possible differential roles of these proteins in vivo are discussed.
Masaki Shintani - One of the best experts on this subject based on the ideXlab platform.
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Table_9_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.XLSX
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
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Data_Sheet_1_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.pdf
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
Kosuke Yanagiya - One of the best experts on this subject based on the ideXlab platform.
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Table_9_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.XLSX
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
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Data_Sheet_1_Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure.pdf
2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide Kimbara, Masaki ShintaniAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments.
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Novel Self-Transmissible and Broad-Host-Range Plasmids Exogenously Captured From Anaerobic Granules or Cow Manure
Frontiers Media S.A., 2018Co-Authors: Kosuke Yanagiya, Yoshiaki Maejima, Hiroki Nakata, Maho Tokuda, Ryota Moriuchi, Hideo Dohra, Kengo Inoue, Moriya Ohkuma, Kazuhide KimbaraAbstract:Novel self-transmissible Plasmids were exogenously captured from environmental samples by triparental matings with pBBR1MCS-2 as a Mobilizable Plasmid and Pseudomonas resinovorans as a recipient. A total of 272 recipients were successfully obtained as Plasmid host candidates from granules of an anaerobic methane fermentation plant and from cow manure. The whole nucleotide sequences of six Plasmids were determined, including one IncP-1 Plasmid (pSN1104-59), four PromA-like Plasmids (pSN1104-11, pSN1104-34, pSN0729-62, and pSN0729-70), and one novel Plasmid (pSN1216-29), whose incompatibility group has not been previously identified. No previously known antibiotic resistance genes were found in these Plasmids. In-depth phylogenetic analyses showed that the PromA-like Plasmids belong to subgroups of PromA (designated as PromAγ and PromAδ) different from previously proposed subgroups PromAα and PromAβ. Twenty-four genes were identified as backbone genes by comparisons with other PromA Plasmids. The nucleotide sequences of pSN1216-29 share high identity with those found in clinical isolates. A minireplicon of pSN1216-29 was successfully constructed from repA encoding a replication initiation protein and oriV. All the captured Plasmids were found to have a broad host range and could be transferred to and replicated in different classes of Proteobacteria. Notably, repA and oriV of pSN1216-29 showed high similarity with one of two replication systems of pSRC119-A/C, known as a Plasmid with multidrug resistance genes found in Salmonella enterica serovar Senftenberg. Our findings suggest that these “cryptic” but broad-host-range Plasmids may be important for spreading several genes as “vehicles” in a wider range of bacteria in natural environments