The Experts below are selected from a list of 54 Experts worldwide ranked by ideXlab platform

Bernard Maitre - One of the best experts on this subject based on the ideXlab platform.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    Background Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. Objectives We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. Methods Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. Results BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 ± 1 mmHg vs. 27 ± 1 mmHg, P ≤ 0.01), right ventricle weight/left ventricle+septum weight ratio (0.29 ± 0.02 vs. 0.36 ± 0.01, P ≤ 0.03), and percentage of muscularized vessels (26.4% vs. 33.5%, P ≤ 0.05), compared to control animals treated with irradiated BMDCs. Tracking cells from constitutive GFP-expressing male donor mice with anti-GFP antibodies or chromosome Y level measurement by quantitative real-time PCR showed BMDCs in the lung. In contrast, chronically hypoxic mice subjected to the same procedure failed to show improvement in PH. Conclusion These results show that BMDCs limit pulmonary vascular remodeling induced by vascular injury but not by hypoxia.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice.
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P

William Raoul - One of the best experts on this subject based on the ideXlab platform.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    Background Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. Objectives We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. Methods Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. Results BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 ± 1 mmHg vs. 27 ± 1 mmHg, P ≤ 0.01), right ventricle weight/left ventricle+septum weight ratio (0.29 ± 0.02 vs. 0.36 ± 0.01, P ≤ 0.03), and percentage of muscularized vessels (26.4% vs. 33.5%, P ≤ 0.05), compared to control animals treated with irradiated BMDCs. Tracking cells from constitutive GFP-expressing male donor mice with anti-GFP antibodies or chromosome Y level measurement by quantitative real-time PCR showed BMDCs in the lung. In contrast, chronically hypoxic mice subjected to the same procedure failed to show improvement in PH. Conclusion These results show that BMDCs limit pulmonary vascular remodeling induced by vascular injury but not by hypoxia.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice.
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P

Saadia Eddahibi - One of the best experts on this subject based on the ideXlab platform.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    Background Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. Objectives We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. Methods Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. Results BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 ± 1 mmHg vs. 27 ± 1 mmHg, P ≤ 0.01), right ventricle weight/left ventricle+septum weight ratio (0.29 ± 0.02 vs. 0.36 ± 0.01, P ≤ 0.03), and percentage of muscularized vessels (26.4% vs. 33.5%, P ≤ 0.05), compared to control animals treated with irradiated BMDCs. Tracking cells from constitutive GFP-expressing male donor mice with anti-GFP antibodies or chromosome Y level measurement by quantitative real-time PCR showed BMDCs in the lung. In contrast, chronically hypoxic mice subjected to the same procedure failed to show improvement in PH. Conclusion These results show that BMDCs limit pulmonary vascular remodeling induced by vascular injury but not by hypoxia.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice.
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P

Serge Adnot - One of the best experts on this subject based on the ideXlab platform.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    Background Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. Objectives We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. Methods Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. Results BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 ± 1 mmHg vs. 27 ± 1 mmHg, P ≤ 0.01), right ventricle weight/left ventricle+septum weight ratio (0.29 ± 0.02 vs. 0.36 ± 0.01, P ≤ 0.03), and percentage of muscularized vessels (26.4% vs. 33.5%, P ≤ 0.05), compared to control animals treated with irradiated BMDCs. Tracking cells from constitutive GFP-expressing male donor mice with anti-GFP antibodies or chromosome Y level measurement by quantitative real-time PCR showed BMDCs in the lung. In contrast, chronically hypoxic mice subjected to the same procedure failed to show improvement in PH. Conclusion These results show that BMDCs limit pulmonary vascular remodeling induced by vascular injury but not by hypoxia.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice.
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P

Anne Hulin - One of the best experts on this subject based on the ideXlab platform.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    Background Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. Objectives We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. Methods Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. Results BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 ± 1 mmHg vs. 27 ± 1 mmHg, P ≤ 0.01), right ventricle weight/left ventricle+septum weight ratio (0.29 ± 0.02 vs. 0.36 ± 0.01, P ≤ 0.03), and percentage of muscularized vessels (26.4% vs. 33.5%, P ≤ 0.05), compared to control animals treated with irradiated BMDCs. Tracking cells from constitutive GFP-expressing male donor mice with anti-GFP antibodies or chromosome Y level measurement by quantitative real-time PCR showed BMDCs in the lung. In contrast, chronically hypoxic mice subjected to the same procedure failed to show improvement in PH. Conclusion These results show that BMDCs limit pulmonary vascular remodeling induced by vascular injury but not by hypoxia.

  • Effects of bone marrow-derived cells on Monocrotaline- and hypoxia-induced pulmonary hypertension in mice.
    Respiratory Research, 2007
    Co-Authors: William Raoul, Orianne Wagner-ballon, Guitanouch Saber, Anne Hulin, Elisabeth Marcos, Stéphane Giraudier, William Vainchenker, Serge Adnot, Saadia Eddahibi, Bernard Maitre
    Abstract:

    BACKGROUND: Bone marrow -derived cells (BMDCs) can either limit or contribute to the process of pulmonary vascular remodeling. Whether the difference in their effects depends on the mechanism of pulmonary hypertension (PH) remains unknown. OBJECTIVES: We investigated the effect of BMDCs on PH induced in mice by either Monocrotaline or exposure to chronic hypoxia. METHODS: Intravenous administration of the active Monocrotaline metabolite (Monocrotaline Pyrrole, MCTp) to C57BL/6 mice induced PH within 15 days, due to remodeling of small distal vessels. Three days after the MCTp injection, the mice were injected with BMDCs harvested from femurs and tibias of donor mice treated with 5-fluorouracil (3.5 mg IP/animal) to deplete mature cells and to allow proliferation of progenitor cells. RESULTS: BMDCs significantly attenuated PH as assessed by reductions in right ventricular systolic pressure (20 +/- 1 mmHg vs. 27 +/- 1 mmHg, P