The Experts below are selected from a list of 285 Experts worldwide ranked by ideXlab platform
Amde Selassie Shifera - One of the best experts on this subject based on the ideXlab platform.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, Jay L Katz, Amde Selassie ShiferaAbstract:Objectives To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, L. Jay Katz, Amde Selassie ShiferaAbstract:Objectives: To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods: Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results: Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions: The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance: These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma. ©2010 American Medical Association.
Jorge A. Alvarado - One of the best experts on this subject based on the ideXlab platform.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, Jay L Katz, Amde Selassie ShiferaAbstract:Objectives To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, L. Jay Katz, Amde Selassie ShiferaAbstract:Objectives: To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods: Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results: Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions: The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance: These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma. ©2010 American Medical Association.
Maciej Siedlar - One of the best experts on this subject based on the ideXlab platform.
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reprint of alterations of trim21 mrna expression during Monocyte maturation
Immunobiology, 2017Co-Authors: Marzena Lenart, Rafal Szatanek, Kazimierz Weglarczyk, Malgorzata Stec, Karolina Bukowskastrakova, Jaroslaw Czyz, Magdalena Rutkowskazapala, Anna Gruca, Maciej SiedlarAbstract:Abstract Tripartite motif-containing protein 21 (TRIM21) play a dual role in the cytoplasm of the cells where it facilitates destruction of some antibody-coated viruses and some bacteria, and initiates synthesis of proinflammatory cytokines. Macrophages and CD16+ Monocyte subset can particularly participate in a proinflammatory response caused by viral infection, however, the molecular mechanisms underlying these processes are not fully understood. The aim of this study was to determine the level of TRIM21-mRNA expression in Monocyte subsets including: classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) Monocytes, as well as during in vitro differentiation of the isolated Monocytes towards dendritic cells or macrophages. Our results revealed that the level of TRIM21 mRNA expression was significantly lower in CD16- Monocytes, when compared to CD16+ cells and the whole Monocyte population, yet no significant differences were observed when CD16+ population was divided into intermediate and non-classical subsets. More pronounced differences were observed in the case of Monocyte-derived macrophages (MDM) and dendritic cells (DCs). TRIM21-mRNA expression level was app. 6-fold higher in DCs, and app. 16-fold higher in MDM (p Our results may suggest the new mechanism of increased proinflammatory cytokine production by CD16+ (intermediate and non-classical) Monocytes and macrophages, at least in patients with acute or chronic infections, caused by enveloped viruses. We suggest that TRIM21 may be one of the factors associated with the “switching on” the proinflammatory programme in CD16+ Monocytes or Monocyte-derived macrophages.
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alterations of trim21 mrna expression during Monocyte maturation
Immunobiology, 2017Co-Authors: Marzena Lenart, Rafal Szatanek, Kazimierz Weglarczyk, Malgorzata Stec, Karolina Bukowskastrakova, Jaroslaw Czyz, Magdalena Rutkowskazapala, Anna Gruca, Maciej SiedlarAbstract:Tripartite motif-containing protein 21 (TRIM21) play a dual role in the cytoplasm of the cells where it facilitates destruction of some antibody-coated viruses and some bacteria, and initiates synthesis of proinflammatory cytokines. Macrophages and CD16+ Monocyte subset can particularly participate in a proinflammatory response caused by viral infection, however, the molecular mechanisms underlying these processes are not fully understood. The aim of this study was to determine the level of TRIM21-mRNA expression in Monocyte subsets including: classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) Monocytes, as well as during in vitro differentiation of the isolated Monocytes towards dendritic cells or macrophages. Our results revealed that the level of TRIM21 mRNA expression was significantly lower in CD16- Monocytes, when compared to CD16+ cells and the whole Monocyte population, yet no significant differences were observed when CD16+ population was divided into intermediate and non-classical subsets. More pronounced differences were observed in the case of Monocyte-derived macrophages (MDM) and dendritic cells (DCs). TRIM21-mRNA expression level was app. 6-fold higher in DCs, and app. 16-fold higher in MDM (p < 0,01), when compared to freshly isolated Monocytes. Our results may suggest the new mechanism of increased proinflammatory cytokine production by CD16+ (intermediate and non-classical) Monocytes and macrophages, at least in patients with acute or chronic infections, caused by enveloped viruses. We suggest that TRIM21 may be one of the factors associated with the “switching on” the proinflammatory programme in CD16+ Monocytes or Monocyte-derived macrophages.
Sheetal Trivedi - One of the best experts on this subject based on the ideXlab platform.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, Jay L Katz, Amde Selassie ShiferaAbstract:Objectives To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma.
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Monocyte modulation of aqueous outflow and recruitment to the trabecular meshwork following selective laser trabeculoplasty
Archives of Ophthalmology, 2010Co-Authors: Jorge A. Alvarado, Sheetal Trivedi, L. Jay Katz, Amde Selassie ShiferaAbstract:Objectives: To determine whether selective laser trabeculoplasty (SLT) induces Monocyte recruitment to the trabecular meshwork (TM) in human and monkey eyes and whether Monocytes increase both aqueous outflow in vivo and the conductivity of human Schlemm canal endothelial cells (SCEs) in vitro. Methods: Monocyte recruitment was examined morphometrically in control human and monkey eyes and compared with that following SLT applied 1 to 3 days earlier. Outflow facility was measured for up to 4 days after the intracameral infusion of autologous macrophages in rabbits. Schlemm canal endothelial cell conductivity was measured using flow meters after exposing cultured SCEs to Monocytes and Monocyte-secreted factors for 24 hours. Results: Our estimates show that the TM in the human eye normally had an average of 15 003 Monocytes, while in the monkey eye there were 3181 Monocytes, and this number increased 4- to 5-fold following SLT. The intracameral infusion of autologous macrophages in rabbits increased outflow facility 2-fold in a rapid and sustained manner. Human Monocytes and Monocyte-secreted factors increased SCE conductivity 2-fold in vitro. Conclusions: The number of Monocytes/macrophages in the TM increases substantially after SLT and Monocytes augment both outflow facility and SCE conductivity. Clinical Relevance: These findings indicate that the innate immune system in general and Monocytes in particular play an important role in aqueous outflow homeostasis. The recruitment of Monocytes in increased numbers after SLT likely plays a role in lowering the intraocular pressure after this procedure. The intracameral introduction of autologous Monocytes harvested from a vein could have therapeutic potential as a cell-based individualized treatment of glaucoma. ©2010 American Medical Association.
Marzena Lenart - One of the best experts on this subject based on the ideXlab platform.
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reprint of alterations of trim21 mrna expression during Monocyte maturation
Immunobiology, 2017Co-Authors: Marzena Lenart, Rafal Szatanek, Kazimierz Weglarczyk, Malgorzata Stec, Karolina Bukowskastrakova, Jaroslaw Czyz, Magdalena Rutkowskazapala, Anna Gruca, Maciej SiedlarAbstract:Abstract Tripartite motif-containing protein 21 (TRIM21) play a dual role in the cytoplasm of the cells where it facilitates destruction of some antibody-coated viruses and some bacteria, and initiates synthesis of proinflammatory cytokines. Macrophages and CD16+ Monocyte subset can particularly participate in a proinflammatory response caused by viral infection, however, the molecular mechanisms underlying these processes are not fully understood. The aim of this study was to determine the level of TRIM21-mRNA expression in Monocyte subsets including: classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) Monocytes, as well as during in vitro differentiation of the isolated Monocytes towards dendritic cells or macrophages. Our results revealed that the level of TRIM21 mRNA expression was significantly lower in CD16- Monocytes, when compared to CD16+ cells and the whole Monocyte population, yet no significant differences were observed when CD16+ population was divided into intermediate and non-classical subsets. More pronounced differences were observed in the case of Monocyte-derived macrophages (MDM) and dendritic cells (DCs). TRIM21-mRNA expression level was app. 6-fold higher in DCs, and app. 16-fold higher in MDM (p Our results may suggest the new mechanism of increased proinflammatory cytokine production by CD16+ (intermediate and non-classical) Monocytes and macrophages, at least in patients with acute or chronic infections, caused by enveloped viruses. We suggest that TRIM21 may be one of the factors associated with the “switching on” the proinflammatory programme in CD16+ Monocytes or Monocyte-derived macrophages.
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alterations of trim21 mrna expression during Monocyte maturation
Immunobiology, 2017Co-Authors: Marzena Lenart, Rafal Szatanek, Kazimierz Weglarczyk, Malgorzata Stec, Karolina Bukowskastrakova, Jaroslaw Czyz, Magdalena Rutkowskazapala, Anna Gruca, Maciej SiedlarAbstract:Tripartite motif-containing protein 21 (TRIM21) play a dual role in the cytoplasm of the cells where it facilitates destruction of some antibody-coated viruses and some bacteria, and initiates synthesis of proinflammatory cytokines. Macrophages and CD16+ Monocyte subset can particularly participate in a proinflammatory response caused by viral infection, however, the molecular mechanisms underlying these processes are not fully understood. The aim of this study was to determine the level of TRIM21-mRNA expression in Monocyte subsets including: classical (CD14++CD16−), intermediate (CD14++CD16+) and non-classical (CD14+CD16++) Monocytes, as well as during in vitro differentiation of the isolated Monocytes towards dendritic cells or macrophages. Our results revealed that the level of TRIM21 mRNA expression was significantly lower in CD16- Monocytes, when compared to CD16+ cells and the whole Monocyte population, yet no significant differences were observed when CD16+ population was divided into intermediate and non-classical subsets. More pronounced differences were observed in the case of Monocyte-derived macrophages (MDM) and dendritic cells (DCs). TRIM21-mRNA expression level was app. 6-fold higher in DCs, and app. 16-fold higher in MDM (p < 0,01), when compared to freshly isolated Monocytes. Our results may suggest the new mechanism of increased proinflammatory cytokine production by CD16+ (intermediate and non-classical) Monocytes and macrophages, at least in patients with acute or chronic infections, caused by enveloped viruses. We suggest that TRIM21 may be one of the factors associated with the “switching on” the proinflammatory programme in CD16+ Monocytes or Monocyte-derived macrophages.
