The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform

Kearney Kate - One of the best experts on this subject based on the ideXlab platform.

  • Using novel approaches to understand development: gene targeting, protein labelling and the development of the accessory glands in Drosophila melanogaster
    2018
    Co-Authors: Kearney Kate
    Abstract:

    Three very different approaches to understanding developmental biology were taken in this project. The first was an attempt to investigate the subCellular localisation of the Drosophila RhoGEF Pebble (Pbl) in live Cells in real time, with an aim to further elucidate the role of Pbl in EMT and mesoderm migration in the embryo. This method, tetracysteine labelling, makes use of a 6 aa tag and a fluorescent, Cell permeable, biarsenical ligand. Pioneered in Roger Tsien's laboratory, it utilises the reversible covalent bonds that form between closely spaced pairs of thiols in the tag and arsenical compounds. However, it was unsuccessful in allowing precise localisation of the Pbl protein either in vitro in Drosophila S2 Cell culture or in vivo in wholemount embryos. While the approach has had success in the mammalian system, particularly mammalian Cell culture, it would appear as though wholemount Drosophila embryos are not amenable to this technology. Secondly, I attempted to delete the PH domain of Pbl using ends out targeted homologous recombination, with an aim to investigate the role of the PH domain in EMT and mesoderm migration in the embryo. The first targeting attempt, to introduce a selectable marker, was successful, with a targeting efficiency of 1 in 160,000. However, the insertion of the PH deletion proved unsuccessful, perhaps due to a small recombination loophole introduced in the first targeting event. Thirdly and finally the development of the binucleate accessory glands was investigated and found to result from a failure of cytokinesis following mitoses that took place between 60 hours and 74 hours after puparium formation. In order to develop tools for the genetic manipulation of this tissue, the expression pattern of two GAL4 drivers, btlGAL4 and prdmf9GAL4, was investigated. prdmf9GAL4 was found to be expressed highly and consistently throughout the binucleate Cell formation period and into the adult gland. Whereas, btlGAL4 was more highly expressed in the associated tracheal tissue of the gland during accessory gland development than in the gland itself. Expression of GAL4 by both drivers induced the formation of Mononucleate accessory gland Cells at different developmental stages, although the reason for this was not elucidated. This Mononucleate Cell type persisted in some Cells of the adult gland in the case of prdmf9GAL4, but were not present in the adult gland in the case of btIGAL4. prdmf9GAL4 presents a potentially useful GAL4 driver for further investigations of the binucleate Cell formation in the accessory glands

Figueiredo F. - One of the best experts on this subject based on the ideXlab platform.

  • Inflammatory response to calcium hydroxide based root canal sealers
    2012
    Co-Authors: Silva, Lea Assed Bezerra, Mário Roberto Leonardo, Faccioli, Lucia Helena, Figueiredo F.
    Abstract:

    This study evaluated the inflammatory response to Sealapex, CRCS, Apexit, and Sealer 26 in the subcutaneous tissue and in peritoneal cavity of Balb/c mice. The inflammatory response of subcutaneous tissue was analyzed after 2, 4, 8, and 16 days. Intense neurotrophilia was seen in response to all sealers during the initial periods. Differences among them related to the presence of necrosis and the number of inflammatory Cells. In the intermediate phase marked differentiation of Cells of the Mononucleate phagocytic system into macrophages, epithelioid Cells and multinucleate giant Cells were observed with Sealapex. This response was less intense with CRCS and Apexit. Tissue necrosis was observed only at tissue sealer interfaces and only during the initial period with Sealapex but was seen throughout the experiment with all other sealers. The animals were injected in the peritoneal cavity with solutions containing the sealers and five mice from each group were killed 6 and 24h, and 5 and 15 days later. During the initial periods (6 and 24h) there was an intense migration of polymorphonuclear leukocytes to the peritoneal cavity in response to all sealers compared to the control. This migration was more intense or Sealer 26 and Apexit. An increase in Mononucleate Cell number was observed after 6 and 24h and 5 days for all sealers and no differences were observed in relation to the control after 15 days (AU

Silva, Lea Assed Bezerra - One of the best experts on this subject based on the ideXlab platform.

  • Inflammatory response to calcium hydroxide based root canal sealers
    2012
    Co-Authors: Silva, Lea Assed Bezerra, Mário Roberto Leonardo, Faccioli, Lucia Helena, Figueiredo F.
    Abstract:

    This study evaluated the inflammatory response to Sealapex, CRCS, Apexit, and Sealer 26 in the subcutaneous tissue and in peritoneal cavity of Balb/c mice. The inflammatory response of subcutaneous tissue was analyzed after 2, 4, 8, and 16 days. Intense neurotrophilia was seen in response to all sealers during the initial periods. Differences among them related to the presence of necrosis and the number of inflammatory Cells. In the intermediate phase marked differentiation of Cells of the Mononucleate phagocytic system into macrophages, epithelioid Cells and multinucleate giant Cells were observed with Sealapex. This response was less intense with CRCS and Apexit. Tissue necrosis was observed only at tissue sealer interfaces and only during the initial period with Sealapex but was seen throughout the experiment with all other sealers. The animals were injected in the peritoneal cavity with solutions containing the sealers and five mice from each group were killed 6 and 24h, and 5 and 15 days later. During the initial periods (6 and 24h) there was an intense migration of polymorphonuclear leukocytes to the peritoneal cavity in response to all sealers compared to the control. This migration was more intense or Sealer 26 and Apexit. An increase in Mononucleate Cell number was observed after 6 and 24h and 5 days for all sealers and no differences were observed in relation to the control after 15 days (AU

Mário Roberto Leonardo - One of the best experts on this subject based on the ideXlab platform.

  • Inflammatory response to calcium hydroxide based root canal sealers
    2012
    Co-Authors: Silva, Lea Assed Bezerra, Mário Roberto Leonardo, Faccioli, Lucia Helena, Figueiredo F.
    Abstract:

    This study evaluated the inflammatory response to Sealapex, CRCS, Apexit, and Sealer 26 in the subcutaneous tissue and in peritoneal cavity of Balb/c mice. The inflammatory response of subcutaneous tissue was analyzed after 2, 4, 8, and 16 days. Intense neurotrophilia was seen in response to all sealers during the initial periods. Differences among them related to the presence of necrosis and the number of inflammatory Cells. In the intermediate phase marked differentiation of Cells of the Mononucleate phagocytic system into macrophages, epithelioid Cells and multinucleate giant Cells were observed with Sealapex. This response was less intense with CRCS and Apexit. Tissue necrosis was observed only at tissue sealer interfaces and only during the initial period with Sealapex but was seen throughout the experiment with all other sealers. The animals were injected in the peritoneal cavity with solutions containing the sealers and five mice from each group were killed 6 and 24h, and 5 and 15 days later. During the initial periods (6 and 24h) there was an intense migration of polymorphonuclear leukocytes to the peritoneal cavity in response to all sealers compared to the control. This migration was more intense or Sealer 26 and Apexit. An increase in Mononucleate Cell number was observed after 6 and 24h and 5 days for all sealers and no differences were observed in relation to the control after 15 days (AU

Faccioli, Lucia Helena - One of the best experts on this subject based on the ideXlab platform.

  • Inflammatory response to calcium hydroxide based root canal sealers
    2012
    Co-Authors: Silva, Lea Assed Bezerra, Mário Roberto Leonardo, Faccioli, Lucia Helena, Figueiredo F.
    Abstract:

    This study evaluated the inflammatory response to Sealapex, CRCS, Apexit, and Sealer 26 in the subcutaneous tissue and in peritoneal cavity of Balb/c mice. The inflammatory response of subcutaneous tissue was analyzed after 2, 4, 8, and 16 days. Intense neurotrophilia was seen in response to all sealers during the initial periods. Differences among them related to the presence of necrosis and the number of inflammatory Cells. In the intermediate phase marked differentiation of Cells of the Mononucleate phagocytic system into macrophages, epithelioid Cells and multinucleate giant Cells were observed with Sealapex. This response was less intense with CRCS and Apexit. Tissue necrosis was observed only at tissue sealer interfaces and only during the initial period with Sealapex but was seen throughout the experiment with all other sealers. The animals were injected in the peritoneal cavity with solutions containing the sealers and five mice from each group were killed 6 and 24h, and 5 and 15 days later. During the initial periods (6 and 24h) there was an intense migration of polymorphonuclear leukocytes to the peritoneal cavity in response to all sealers compared to the control. This migration was more intense or Sealer 26 and Apexit. An increase in Mononucleate Cell number was observed after 6 and 24h and 5 days for all sealers and no differences were observed in relation to the control after 15 days (AU