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Eric N Jellen - One of the best experts on this subject based on the ideXlab platform.
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Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat
Theoretical and Applied Genetics, 2001Co-Authors: S. L. Fox, Eric N Jellen, H W Rines, Shahryar F. Kianian, R L PhillipsAbstract:The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population. Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes. In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally, the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.
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Characterization of ‘Sun II’ oat Monosomics through C-banding and identification of eight new ‘Sun II’ Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, S. L. Fox, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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characterization of sun ii oat Monosomics through c banding and identification of eight new sun ii Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
Theoretical and Applied Genetics, 1994Co-Authors: William L Rooney, Eric N Jellen, Ronald L Phillips, H W Rines, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.
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characterization of the hexaploid oat avena byzantina cv kanota monosomic series using c banding and rflps
Genome, 1993Co-Authors: Eric N Jellen, William L Rooney, Ronald L Phillips, H W RinesAbstract:The establishment of a C-banded karyotype of hexaploid oat (Avena spp., 2n = 6x = 42) has facilitated the cytological characterization of a monosomic series in 'Kanota', an A. byzantina (C. Koch) cultivar. The 'Kanota' series of Monosomics analyzed in this study consists of only 12 of the 21 different chromosome-deficient lines possible plus potential translocated segments of two or three additional chromosomes. These findings were confirmed by RFLP mapping data from studies in which oat probes were assigned to syntenic groups using the 'Kanota' set of monosomic lines. Among the remaining nine monosomic lines analyzed, eight are missing chromosomes represented in the set of 12 unique lines and one line, monosomic K13, is missing a chromosome from the unique set of 12 that possesses a cytologically detectable translocation. This same translocation, involving chromosomes 7C and 14, is found in 5 of the 21 'Kanota' Monosomics. The incompleteness of the set of 'Kanota' Monosomics might be due to (i) difficult...
H W Rines - One of the best experts on this subject based on the ideXlab platform.
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Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat
Theoretical and Applied Genetics, 2001Co-Authors: S. L. Fox, Eric N Jellen, H W Rines, Shahryar F. Kianian, R L PhillipsAbstract:The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population. Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes. In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally, the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.
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characterization of sun ii oat Monosomics through c banding and identification of eight new sun ii Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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Characterization of ‘Sun II’ oat Monosomics through C-banding and identification of eight new ‘Sun II’ Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, S. L. Fox, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
Theoretical and Applied Genetics, 1994Co-Authors: William L Rooney, Eric N Jellen, Ronald L Phillips, H W Rines, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.
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Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1994Co-Authors: W L Rooney, H W Rines, E N Jellen, R L Phillips, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.Mention of a trademark or proprietary product does not constitute a guarantee or warranty by the USDA-ARS or the University of Minnesota and does not imply approval over other products that also may be suitable.
B S Gill - One of the best experts on this subject based on the ideXlab platform.
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characterization of sun ii oat Monosomics through c banding and identification of eight new sun ii Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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Characterization of ‘Sun II’ oat Monosomics through C-banding and identification of eight new ‘Sun II’ Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, S. L. Fox, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
Ronald L Phillips - One of the best experts on this subject based on the ideXlab platform.
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Characterization of ‘Sun II’ oat Monosomics through C-banding and identification of eight new ‘Sun II’ Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, S. L. Fox, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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characterization of sun ii oat Monosomics through c banding and identification of eight new sun ii Monosomics
Theoretical and Applied Genetics, 1997Co-Authors: Eric N Jellen, Ronald L Phillips, H W Rines, D W Davis, B S GillAbstract:Monosomics are a powerful tool for genetic mapping in allopolyploid plant species such as oat (Avena sativa L., 2n=6x=42). A C-banded karyotype of the oat cultivar Sun II was compared with previously described oat karyotypes and was used to identify the missing chromosome in each line of Sun II aneuploids. These included new aneuploids, isolated among derivatives of oat haploids obtained from Sun II oat×maize crosses, along with the original Sun II aneuploid set which had been obtained by cytological screening of a Sun II population for spontaneous aneuploids. Eight new Sun II Monosomics were identified among the derivatives of haploids from the oat×maize crosses, to give a total of 18 unique Sun II monosomic/nullisomic lines. All seven C-genome chromosomes are represented by Sun II Monosomics. Chromosomes 13, 14 and 17 are not represented by Sun II aneuploids but are found in the Kanota monosomic series. Therefore, Monosomics of some form are now available for all 21 oat chromosomes. A reciprocal translocation involving chromosomes 3C and 14, found in a portion of the original set of Sun II monosomic lines, was also described. No new translocations were detected in the Sun II×maize crosses.
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Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
Theoretical and Applied Genetics, 1994Co-Authors: William L Rooney, Eric N Jellen, Ronald L Phillips, H W Rines, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.
-
characterization of the hexaploid oat avena byzantina cv kanota monosomic series using c banding and rflps
Genome, 1993Co-Authors: Eric N Jellen, William L Rooney, Ronald L Phillips, H W RinesAbstract:The establishment of a C-banded karyotype of hexaploid oat (Avena spp., 2n = 6x = 42) has facilitated the cytological characterization of a monosomic series in 'Kanota', an A. byzantina (C. Koch) cultivar. The 'Kanota' series of Monosomics analyzed in this study consists of only 12 of the 21 different chromosome-deficient lines possible plus potential translocated segments of two or three additional chromosomes. These findings were confirmed by RFLP mapping data from studies in which oat probes were assigned to syntenic groups using the 'Kanota' set of monosomic lines. Among the remaining nine monosomic lines analyzed, eight are missing chromosomes represented in the set of 12 unique lines and one line, monosomic K13, is missing a chromosome from the unique set of 12 that possesses a cytologically detectable translocation. This same translocation, involving chromosomes 7C and 14, is found in 5 of the 21 'Kanota' Monosomics. The incompleteness of the set of 'Kanota' Monosomics might be due to (i) difficult...
Shahryar F. Kianian - One of the best experts on this subject based on the ideXlab platform.
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Assignment of RFLP linkage groups to chromosomes using monosomic F1 analysis in hexaploid oat
Theoretical and Applied Genetics, 2001Co-Authors: S. L. Fox, Eric N Jellen, H W Rines, Shahryar F. Kianian, R L PhillipsAbstract:The availability of molecular genetic maps in oat (Avena spp.) and improved identification of chromosomes by C-banding are two recent developments that have made locating linkage groups to chromosomes possible in cultivated hexaploid oat, 2n=6x=42. Monosomic series derived from Avena byzantina C. Koch cv Kanota and from Avena sativa L. cv Sun II were used as maternal plants in crosses with the parents, Kanota-1 and Ogle-C, of the oat RFLP mapping population. Monosomic F1 plants were identified by root-tip cell chromosome counts. For marker analysis, DNAs of eight F2 plants from a monosomic F1 were combined to provide a larger source of DNA that mimicked that of the monosomic F1 plant. Absence of maternal alleles in monosomic F1s served to associate linkage groups with individual chromosomes. Twenty two linkage groups were associated with 16 chromosomes. In seven instances, linkage groups that were independent of each other in recombination analyses were associated with the same chromosome. Five linkage groups were shown to be associated with translocation differences among oat lines. Additionally, the results better-characterized the oat monosomic series through the detection of duplicates and translocation differences among the various monosomic lines. The F1 monosomic series represents a powerful cytogenetic tool with the potential to greatly improve understanding of the oat genome.
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Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
Theoretical and Applied Genetics, 1994Co-Authors: William L Rooney, Eric N Jellen, Ronald L Phillips, H W Rines, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.
-
Identification of homoeologous chromosomes in hexaploid oat (A. byzantina cv Kanota) using Monosomics and RFLP analysis.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 1994Co-Authors: W L Rooney, H W Rines, E N Jellen, R L Phillips, Shahryar F. KianianAbstract:The use of RFLP markers, together with a partial set of Monosomics available in Avena byzantina cv Kanota, has enabled us to identify putative homoeologous chromosome sets in hexaploid Avena species (2n = 6x = 42, AACCDD). We first identified probes producing distinct three-band patterns on Southern blots that possibly reflect orthologous loci of the three genomes present in the hexaploid. Using monosomic analysis, 51 different restriction fragments that hybridized to 26 probes were localized to 12 different chromosomes for which monosomic stocks were available. These DNA restriction fragments were localized to specific Monosomics using image analysis to quantify band intensity relative to other bands in the same lane. From these data, we have tentatively identified two complete homoeologous sets of three chromosomes each and two partial sets of two of the three chromosomes. The results indicate that RFLP dosage analysis is useful in the characterization of homoeologous chromosomes in hexaploid oat where nullisomics for many of the chromosomes are not available.Mention of a trademark or proprietary product does not constitute a guarantee or warranty by the USDA-ARS or the University of Minnesota and does not imply approval over other products that also may be suitable.