The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Shuyao Wang - One of the best experts on this subject based on the ideXlab platform.
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antiviral activity against enterovirus 71 of sulfated rhamnan isolated from the green alga Monostroma latissimum
Carbohydrate Polymers, 2018Co-Authors: Shuyao Wang, Wei Wang, Yu Yunjia, Meijia HeAbstract:Abstract Polysaccharide from Monostroma latissimum PML is a sulfated rhamnan, which consists of →3)-α-L-Rhap-(1→ and →2)-α-L-Rhap-(1→ residues with partial branches and sulfate groups at C-2 of →3)-α-L-Rhap-(1→ and/or C-3 of →2)-α-L-Rhap-(1→. The anti-enterovirus 71 (EV71) activity in vitro of PML was assessed by cytopathic effect inhibition and plaque reduction assays, and the results showed that PML was non-cytotoxic and significantly inhibited EV71 infection. The mechanism analysis of anti-EV71 activity demonstrated that PML largely inhibited viral replication before or during viral adsorption, mainly by targeting the capsid protein VP1. PML may also inhibit some early steps of infection after viral adsorption by modulating signaling through the epidermal growth factor receptor (EGFR)/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Moreover, PML markedly improved survival and decreased viral titers in EV71-infected mice. The investigation revealed that PML has potential as a novel anti-EV71 agent targeting the viral capsid protein as well as cellular EGFR/PI3K/Akt pathway.
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a rhamnan type sulfated polysaccharide with novel structure from Monostroma angicava kjellm chlorophyta and its bioactivity
Carbohydrate Polymers, 2017Co-Authors: Xiaoxi He, Shuyao WangAbstract:Abstract A homogeneous polysaccharide was obtained from Monostroma angicava Kjellm by water extraction, preparative anion-exchange and size-exclusion chromatography. Results of chemical and spectroscopic analyses showed that the polysaccharide was a glucuronic acid-containing rhamnan-type sulfated polysaccharide. The backbone mainly consisted of →3)-α- l - R ha p -(1→ and →2)-α- l - R ha p -(1→ residues, partially sulfated at C-2 of →3)-α- l - R ha p -(1→ and C-3/C-4 of →2)-α- l - R ha p -(1→. The branching contained unsulfated or monosulfated 3-linked, 2-linked, 4-linked α- l -rhamnose and terminal β- d -glucuronic acid residues. The polysaccharide had strong antidiabetic activity assessed by glucose consumption, total cholesterol and triglyceride levels using human hepatocellular carcinoma (HepG2) and insulin-resistant HepG2 cells. The polysaccharide exhibited high anticoagulant property by activated partial thromboplastin time and thrombin time assays, and possessed high fibrin(ogen)olytic activity evaluated by plasminogen activator inhibitior-1, fibrin(ogen) degradation products and D-dimer levels using rats plasma. The investigation demonstrated that the polysaccharide from Monostroma angicava Kjellm was a novel sulfated rhamnan and could be a potential antidiabetic and anticoagulant polysaccharide.
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structure and anticoagulant property of a sulfated polysaccharide isolated from the green seaweed Monostroma angicava
Carbohydrate Polymers, 2017Co-Authors: Na Li, Xiaoxi He, Shuyao Wang, Huali XianAbstract:Abstract An anticoagulant-active polysaccharide PF2 was extracted with boiling water from the green seaweed Monostroma angicava , further purified by anion-exchange and size-exclusion chromatography. PF2 was a rhamnan-type sulfated polysaccharide with molecular weight of about 88.1 kDa. Results of chemical and spectroscopic analyses demonstrated that PF2 consisted of → 3)-α- l - R ha p -(1→ and →2)-α- l - R ha p -(1 → residues, with partially branches at C-2 of → 3)-α- l - R ha p -(1 → residues. Sulfate groups were substituted at C-3 of → 2)-α- l - R ha p -(1 → residues. The sulfated polysaccharide PF2 had a high anticoagulant action, and the mechanism of anticoagulant activity mediated by PF2 was mainly attributed to strong potentiation thrombin by heparin cofactor II. PF2 also exhibited weak effect on antithrombin-dependent thrombin or factor Xa inhibition. The fibrin(ogen)olytic activity and thrombolytic activity of PF2 were also evaluated. The investigation revealed that PF2 was a novel sulfated rhamnan differing from previously described sulfated polysaccharides from green seaweed and could be a potential anticoagulant polysaccharide.
Yin Chen - One of the best experts on this subject based on the ideXlab platform.
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preparation structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum
Bioresource Technology, 2012Co-Authors: Hongyan Li, Xiaohui Qi, Yin Chen, Chunqi Zhao, Yanli Chen, Na Li, Chunyan WangAbstract:Abstract A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked α- l -rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked α- l -rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked α- l -rhamnose and 1,2-linked α- l -rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity.
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chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum chlorophyta
International Journal of Biological Macromolecules, 2009Co-Authors: Hongyan Li, Yi Li, Huijuan Zhang, Xiaohui Qi, Yin ChenAbstract:A polysaccharide was isolated from marine green algae Monostroma latissimum, and its chemical characteristic and anticoagulant activity were investigated. The results demonstrated that the polysaccharide was high rhamnose-containing sulfated polysaccharide, and was mainly composed of 1,2-linked l-rhamnose residues with sulfate groups substituted at positions C-3 and/or C-4. The sulfated polysaccharide exhibited high anticoagulant activities by assays of the activated partial thromboplastin time (APTT) and thrombin time (TT). The anticoagulant property of the sulfated polysaccharide was mainly attributed to powerful potentiation thrombin by heparin cofactor II.
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heparinoid active two sulfated polysaccharides isolated from marine green algae Monostroma nitidum
Carbohydrate Polymers, 2008Co-Authors: Fang Fang, Hongyan Li, Xiaohui Qi, Yin ChenAbstract:Two sulfated polysaccharides WF1 and WF3 were isolated from marine green algae Monostroma nitidum, and their structural characteristics were determined. Anticoagulant activities of WF1 and WF3 were evaluated by assays of the activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), antithrombin and anticoagulation factor Xa activities. The results showed that WF1 and WF3 had similar high contents of rhamnose, whereas their sulfate contents, sulfation positions, molecular sizes and linkage patterns of rhamnose residues were different. The bioassay results demonstrated that WF1 and WF3 had high anticoagulant activities, and were potent thrombin inhibitors mediated by heparin cofactor II, especially WF3. They also hastened thrombin and coagulation factor Xa inhibition by potentiating antithrombin III, but at a lower effectiveness. The differences of anticoagulant activities between WF1 and WF3 were directly due to their structural features discrepancy.
Rong Huei Chen - One of the best experts on this subject based on the ideXlab platform.
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Seed galactomannans: An overview
Carbohydrate Polymers, 2009Co-Authors: Rong Huei Chen, Weei Yuu Chen, H. P. Ramesh, La Plata, Buenos Aires, Barrio La, V. P. Kapoor, Manjoosha Srivastava, Rudrapatnam N. Tharanathan, J. C. Onweluzo, Lovedeep Kaur, Jaspreet SinghAbstract:A study was made to explore the rheological properties of the water-soluble mucilage of a green laver, Monostroma nitidium. The apparent viscosity of the water-soluble mucilage decreased with increasing shear rate, with increasing solution temperature (from 4 to 70 degreesC), with increasing NaCl concentration from (0.5% to 2.0%), and decreased slightly with a shearing time for 60 min. The decreases in the apparent viscosity were more pronounced for higher-concentration water-soluble mucilage. However, it increased with increasing solution pH from pH 3 to 9.
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Rheological properties of the water-soluble mucilage of a green laver, Monostroma nitidium
Journal of Applied Phycology, 2001Co-Authors: Rong Huei Chen, Weei Yuu ChenAbstract:A study was made to explore the rheological properties of the water-solublemucilage of a green laver, Monostroma nitidium . The apparentviscosity of the water-soluble mucilage decreased with increasing shear rate,with increasing solution temperature (from 4 to 70 °C), withincreasing NaCl concentration from (0.5% to 2.0%), and decreasedslightly with a shearing time for 60 min. The decreases in the apparentviscosity were more pronounced for higher-concentration water-solublemucilage. However, it increased with increasing solution pH from pH 3 to 9.
Hongyan Li - One of the best experts on this subject based on the ideXlab platform.
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preparation structure and anticoagulant activity of a low molecular weight fraction produced by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum
Bioresource Technology, 2012Co-Authors: Hongyan Li, Xiaohui Qi, Yin Chen, Chunqi Zhao, Yanli Chen, Na Li, Chunyan WangAbstract:Abstract A low molecular weight fraction, designated LMWP, was prepared by mild acid hydrolysis of sulfated rhamnan from Monostroma latissimum and purified by anion-exchange and gel-permeation chromatography. Chemical and spectroscopic analyses showed that LMWP was mainly composed of rhamnose, and its molecular weight was about 33.6 kDa. The backbone of LMWP consists of 1,3-linked α- l -rhamnose units with partially sulfate groups at the C-2 position. Approximately 25% of 1,3-linked α- l -rhamnose units is substituted at C-2 by sulfated or non-sulfated 1,3-linked α- l -rhamnose and 1,2-linked α- l -rhamnose units. LMWP effectively prolonged clotting time as evaluated by the activated partial thromboplastin time assay and was a potent thrombin inhibitor mediated by heparin cofactor II. The investigation demonstrated that LMWP is a novel sulfated polysaccharide with anticoagulant activity.
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chemical characteristic and anticoagulant activity of the sulfated polysaccharide isolated from Monostroma latissimum chlorophyta
International Journal of Biological Macromolecules, 2009Co-Authors: Hongyan Li, Yi Li, Huijuan Zhang, Xiaohui Qi, Yin ChenAbstract:A polysaccharide was isolated from marine green algae Monostroma latissimum, and its chemical characteristic and anticoagulant activity were investigated. The results demonstrated that the polysaccharide was high rhamnose-containing sulfated polysaccharide, and was mainly composed of 1,2-linked l-rhamnose residues with sulfate groups substituted at positions C-3 and/or C-4. The sulfated polysaccharide exhibited high anticoagulant activities by assays of the activated partial thromboplastin time (APTT) and thrombin time (TT). The anticoagulant property of the sulfated polysaccharide was mainly attributed to powerful potentiation thrombin by heparin cofactor II.
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heparinoid active two sulfated polysaccharides isolated from marine green algae Monostroma nitidum
Carbohydrate Polymers, 2008Co-Authors: Fang Fang, Hongyan Li, Xiaohui Qi, Yin ChenAbstract:Two sulfated polysaccharides WF1 and WF3 were isolated from marine green algae Monostroma nitidum, and their structural characteristics were determined. Anticoagulant activities of WF1 and WF3 were evaluated by assays of the activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), antithrombin and anticoagulation factor Xa activities. The results showed that WF1 and WF3 had similar high contents of rhamnose, whereas their sulfate contents, sulfation positions, molecular sizes and linkage patterns of rhamnose residues were different. The bioassay results demonstrated that WF1 and WF3 had high anticoagulant activities, and were potent thrombin inhibitors mediated by heparin cofactor II, especially WF3. They also hastened thrombin and coagulation factor Xa inhibition by potentiating antithrombin III, but at a lower effectiveness. The differences of anticoagulant activities between WF1 and WF3 were directly due to their structural features discrepancy.
Weei Yuu Chen - One of the best experts on this subject based on the ideXlab platform.
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Seed galactomannans: An overview
Carbohydrate Polymers, 2009Co-Authors: Rong Huei Chen, Weei Yuu Chen, H. P. Ramesh, La Plata, Buenos Aires, Barrio La, V. P. Kapoor, Manjoosha Srivastava, Rudrapatnam N. Tharanathan, J. C. Onweluzo, Lovedeep Kaur, Jaspreet SinghAbstract:A study was made to explore the rheological properties of the water-soluble mucilage of a green laver, Monostroma nitidium. The apparent viscosity of the water-soluble mucilage decreased with increasing shear rate, with increasing solution temperature (from 4 to 70 degreesC), with increasing NaCl concentration from (0.5% to 2.0%), and decreased slightly with a shearing time for 60 min. The decreases in the apparent viscosity were more pronounced for higher-concentration water-soluble mucilage. However, it increased with increasing solution pH from pH 3 to 9.
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Rheological properties of the water-soluble mucilage of a green laver, Monostroma nitidium
Journal of Applied Phycology, 2001Co-Authors: Rong Huei Chen, Weei Yuu ChenAbstract:A study was made to explore the rheological properties of the water-solublemucilage of a green laver, Monostroma nitidium . The apparentviscosity of the water-soluble mucilage decreased with increasing shear rate,with increasing solution temperature (from 4 to 70 °C), withincreasing NaCl concentration from (0.5% to 2.0%), and decreasedslightly with a shearing time for 60 min. The decreases in the apparentviscosity were more pronounced for higher-concentration water-solublemucilage. However, it increased with increasing solution pH from pH 3 to 9.