The Experts below are selected from a list of 1434 Experts worldwide ranked by ideXlab platform
Dennis C. Westhoff - One of the best experts on this subject based on the ideXlab platform.
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Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in a Recirculating System
Journal of food protection, 1997Co-Authors: Patti C. Nedoluha, Dennis C. WesthoffAbstract:The skin, gills, intestines, tank water, and diet of striped bass grown in recirculating tanks were examined for quantity and quality of microflora. Plate counts for the fish and water samples were similar to counts reported in other systems. The bacteria groups represented most frequently in isolates from the fish were Aeromonas (14%), Moraxellaceae (15%), the Flavobacterium - Chryseobacterium - Cytophaga - Sphingobacterium group (11 %), Bacillus (8%), Group I Pseudomonas (6%), the Shewanella - Alteromonas group (6%), and Comamonadaceae (5%). The food-borne pathogens Staphylococcus aureus , motile Aeromonas spp., and Vibrio mimicus were isolated repeatedly. V. parahaemolyticus and V. cholera were each isolated once, and no Listeria , Plesiomonas , Salmonella , or Yersinia enterocolitica were isolated. Close taxonomic relationships were observed among some of the bacteria found on the fish and in the water; bacteria from the skin and gills were more likely to be related to each other and to the water isolates than to the intestinal isolates. Some bacteria in the intestines originated in the diet, but these strains did not significantly affect the overall microflora of the fish and water.
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Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in Flow-Through Tanks
Journal of food protection, 1995Co-Authors: Patti C. Nedoluha, Dennis C. WesthoffAbstract:Aquacultured striped bass from indoor flow-through tanks were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7°C, 22°C, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild striped bass and pond-raised hybrid striped bass. The predominant groups of bacteria isolated were Aeromonas spp. (18%), Flavobacterium/Cytophaga species (15%), Comamonadaceae (15%), Plesiomonas shigelloides (13%), Moraxellaceae (6%), Bacillus spp. (4%), and an unidentified gram-negative rod (9%). Foodborne pathogens Staphylococcus aureus and Vibrio spp. were also isolated.
Patti C. Nedoluha - One of the best experts on this subject based on the ideXlab platform.
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Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in a Recirculating System
Journal of food protection, 1997Co-Authors: Patti C. Nedoluha, Dennis C. WesthoffAbstract:The skin, gills, intestines, tank water, and diet of striped bass grown in recirculating tanks were examined for quantity and quality of microflora. Plate counts for the fish and water samples were similar to counts reported in other systems. The bacteria groups represented most frequently in isolates from the fish were Aeromonas (14%), Moraxellaceae (15%), the Flavobacterium - Chryseobacterium - Cytophaga - Sphingobacterium group (11 %), Bacillus (8%), Group I Pseudomonas (6%), the Shewanella - Alteromonas group (6%), and Comamonadaceae (5%). The food-borne pathogens Staphylococcus aureus , motile Aeromonas spp., and Vibrio mimicus were isolated repeatedly. V. parahaemolyticus and V. cholera were each isolated once, and no Listeria , Plesiomonas , Salmonella , or Yersinia enterocolitica were isolated. Close taxonomic relationships were observed among some of the bacteria found on the fish and in the water; bacteria from the skin and gills were more likely to be related to each other and to the water isolates than to the intestinal isolates. Some bacteria in the intestines originated in the diet, but these strains did not significantly affect the overall microflora of the fish and water.
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Microbiological Analysis of Striped Bass (Morone saxatilis) Grown in Flow-Through Tanks
Journal of food protection, 1995Co-Authors: Patti C. Nedoluha, Dennis C. WesthoffAbstract:Aquacultured striped bass from indoor flow-through tanks were examined for quantity and quality of microflora. Plate counts were performed at three temperatures (7°C, 22°C, and 35°C) under aerobic and anaerobic conditions. Bacterial loads on the skin, gills, and intestines were similar to those reported for wild striped bass and pond-raised hybrid striped bass. The predominant groups of bacteria isolated were Aeromonas spp. (18%), Flavobacterium/Cytophaga species (15%), Comamonadaceae (15%), Plesiomonas shigelloides (13%), Moraxellaceae (6%), Bacillus spp. (4%), and an unidentified gram-negative rod (9%). Foodborne pathogens Staphylococcus aureus and Vibrio spp. were also isolated.
Janet I Macinnes - One of the best experts on this subject based on the ideXlab platform.
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The microbiome of the soft palate of swine.
Animal Health Research Reviews, 2012Co-Authors: Shaun Kernaghan, Adina R Bujold, Janet I MacinnesAbstract:Abstract The tonsil of the soft palate in pigs is a secondary lymphoid tissue that provides a first line ofdefense against foreign antigens entering by the mouth or nares. It has been known for a longtimetobe thesiteofcolonization of important swine andzoonotic bacterial pathogens. Initiallyour understanding of microbes present at this site came from culture-based studies. Veryrecently, sequence-based approaches have been used to identify the core microbiome of theswine tonsil. Although animal to animal and herd to herd variation was detected in thesestudies, >90 of the organisms detected belonged to the phyla Proteobacteria and Firmicutes.Members of the family Pasteurellaceae appeared to be predominate in the tonsil; however, therelative proportions of Actinobacillus, Haemophilus, and Pasteurella varied. Members of thefamilies Moraxellaceae, Fusobacteriaceae, Veillonellaceae, and Neisseriaceae were also seen asfrequent residents of the tonsil. Keywords: swine, tonsil of the soft palate, microbiome, microbiota, palatine tonsil/microbiology
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the microbiome of the soft palate of swine
Animal Health Research Reviews, 2012Co-Authors: Shaun Kernaghan, Adina R Bujold, Janet I MacinnesAbstract:The tonsil of the soft palate in pigs is a secondary lymphoid tissue that provides a first line of defense against foreign antigens entering by the mouth or nares. It has been known for a long time to be the site of colonization of important swine and zoonotic bacterial pathogens. Initially our understanding of microbes present at this site came from culture-based studies. Very recently, sequence-based approaches have been used to identify the core microbiome of the swine tonsil. Although animal to animal and herd to herd variation was detected in these studies, >90 of the organisms detected belonged to the phyla Proteobacteria and Firmicutes. Members of the family Pasteurellaceae appeared to be predominate in the tonsil; however, the relative proportions of Actinobacillus, Haemophilus, and Pasteurella varied. Members of the families Moraxellaceae, Fusobacteriaceae, Veillonellaceae, and Neisseriaceae were also seen as frequent residents of the tonsil.
Andanthony B. Schryvers - One of the best experts on this subject based on the ideXlab platform.
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a comparative cross species investigation of the properties and roles of transferrin and lactoferrin binding protein b from pathogenic bacteria
Biochemistry and Cell Biology, 2017Co-Authors: Nicholas Ostan, Ari Morgenthau, Ronghua Yu, Scott D Grayowen, Andanthony B. SchryversAbstract:Pathogenic bacteria from the families Neisseriaeceae and Moraxellaceae acquire iron from their host using surface receptors that have the ability to hijack iron from the iron-sequestering host proteins transferrin (Tf) and lactoferrin (Lf). The process of acquiring iron from Tf has been well-characterized, including the role of the surface lipoprotein transferrin-binding protein B (TbpB). In contrast, the only well-defined role for the homologue, LbpB, is in its protection against cationic antimicrobial peptides, which is mediated by regions present in some LbpBs that are highly enriched in glutamic or aspartic acid. In this study we compare the Tf-TbpB and the Lf-LbpB interactions and examine the protective effect of LbpB against extracts from human and transgenic mouse neutrophils to gains insights into the physiological roles of LbpB. The results indicate that in contrast to the Tf-TbpB interaction, Lf-LbpB interaction is sensitive to pH and varies between species. In addition, the results with transge...
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A comparative, cross-species investigation of the properties and roles of transferrin- and lactoferrin-binding protein B from pathogenic bacteria.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2016Co-Authors: Nicholas Ostan, Scott D. Gray-owen, Ari Morgenthau, Andanthony B. SchryversAbstract:Pathogenic bacteria from the families Neisseriaeceae and Moraxellaceae acquire iron from their host using surface receptors that have the ability to hijack iron from the iron-sequestering host proteins transferrin (Tf) and lactoferrin (Lf). The process of acquiring iron from Tf has been well-characterized, including the role of the surface lipoprotein transferrin-binding protein B (TbpB). In contrast, the only well-defined role for the homologue, LbpB, is in its protection against cationic antimicrobial peptides, which is mediated by regions present in some LbpBs that are highly enriched in glutamic or aspartic acid. In this study we compare the Tf-TbpB and the Lf-LbpB interactions and examine the protective effect of LbpB against extracts from human and transgenic mouse neutrophils to gains insights into the physiological roles of LbpB. The results indicate that in contrast to the Tf-TbpB interaction, Lf-LbpB interaction is sensitive to pH and varies between species. In addition, the results with transgenic mouse neutrophils raise the question of whether there is species specificity in the cleavage of Lf to generate cationic antimicrobial peptides or differences in the potency of peptides derived from mouse and human Lf.
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crystal structure of the n lobe of lactoferrin binding protein b from moraxella bovis
Biochemistry and Cell Biology, 2012Co-Authors: Elena Arutyunova, Amanda J. Beddek, Andanthony B. Schryvers, Cory L Brooks, M J LemieuxAbstract:Lactoferrin (Lf) is a bi-lobed, iron-binding protein found on mucosal surfaces and at sites of inflammation. Gram-negative pathogens from the Neisseriaceae and Moraxellaceae families are capable of using Lf as a source of iron for growth through a process mediated by a bacterial surface receptor that directly binds host Lf. This receptor consists of an integral outer membrane protein, lactoferrin binding protein A (LbpA), and a surface lipoprotein, lactoferrin binding protein B (LbpB). The N-lobe of the homologous transferrin binding protein B, TbpB, has been shown to facilitate transferrin binding in the process of iron acquisition. Currently there is little known about the role of LbpB in iron acquisition or how Lf interacts with the bacterial receptor proteins. No structural information on any LbpB or domain is available. In this study, we express and purify from Escherichia coli the full-length LbpB and the N-lobe of LbpB from the bovine pathogen Moraxella bovis for crystallization trials. We demonstr...
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Crystal structure of the N-lobe of lactoferrin binding protein B from Moraxella bovis.
Biochemistry and cell biology = Biochimie et biologie cellulaire, 2012Co-Authors: Elena Arutyunova, Amanda J. Beddek, Andanthony B. Schryvers, Cory L Brooks, Michelle W. Mak, M J LemieuxAbstract:Lactoferrin (Lf) is a bi-lobed, iron-binding protein found on mucosal surfaces and at sites of inflammation. Gram-negative pathogens from the Neisseriaceae and Moraxellaceae families are capable of using Lf as a source of iron for growth through a process mediated by a bacterial surface receptor that directly binds host Lf. This receptor consists of an integral outer membrane protein, lactoferrin binding protein A (LbpA), and a surface lipoprotein, lactoferrin binding protein B (LbpB). The N-lobe of the homologous transferrin binding protein B, TbpB, has been shown to facilitate transferrin binding in the process of iron acquisition. Currently there is little known about the role of LbpB in iron acquisition or how Lf interacts with the bacterial receptor proteins. No structural information on any LbpB or domain is available. In this study, we express and purify from Escherichia coli the full-length LbpB and the N-lobe of LbpB from the bovine pathogen Moraxella bovis for crystallization trials. We demonstr...
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anchor peptide of transferrin binding protein b is required for interaction with transferrin binding protein a
Journal of Biological Chemistry, 2011Co-Authors: Xue Yang, Charles Calmettes, Trevor F Moraes, Ronghua Yu, Andanthony B. SchryversAbstract:Gram-negative bacterial pathogens belonging to the Pasteurellaceae, Moraxellaceae, and Neisseriaceae families rely on an iron acquisition system that acquires iron directly from host transferrin (Tf). The process is mediated by a surface receptor composed of transferrin-binding proteins A and B (TbpA and TbpB). TbpA is an integral outer membrane protein that functions as a gated channel for the passage of iron into the periplasm. TbpB is a surface-exposed lipoprotein that facilitates the iron uptake process. In this study, we demonstrate that the region encompassing amino acids 7–40 of Actinobacillus pleuropneumoniae TbpB is required for forming a complex with TbpA and that the formation of the complex requires the presence of porcine Tf. These results are consistent with a model in which TbpB is responsible for the initial capture of iron-loaded Tf and subsequently interacts with TbpA through the anchor peptide. We propose that TonB binding to TbpA initiates the formation of the TbpB-TbpA complex and transfer of Tf to TbpA.
Shaun Kernaghan - One of the best experts on this subject based on the ideXlab platform.
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The microbiome of the soft palate of swine.
Animal Health Research Reviews, 2012Co-Authors: Shaun Kernaghan, Adina R Bujold, Janet I MacinnesAbstract:Abstract The tonsil of the soft palate in pigs is a secondary lymphoid tissue that provides a first line ofdefense against foreign antigens entering by the mouth or nares. It has been known for a longtimetobe thesiteofcolonization of important swine andzoonotic bacterial pathogens. Initiallyour understanding of microbes present at this site came from culture-based studies. Veryrecently, sequence-based approaches have been used to identify the core microbiome of theswine tonsil. Although animal to animal and herd to herd variation was detected in thesestudies, >90 of the organisms detected belonged to the phyla Proteobacteria and Firmicutes.Members of the family Pasteurellaceae appeared to be predominate in the tonsil; however, therelative proportions of Actinobacillus, Haemophilus, and Pasteurella varied. Members of thefamilies Moraxellaceae, Fusobacteriaceae, Veillonellaceae, and Neisseriaceae were also seen asfrequent residents of the tonsil. Keywords: swine, tonsil of the soft palate, microbiome, microbiota, palatine tonsil/microbiology
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the microbiome of the soft palate of swine
Animal Health Research Reviews, 2012Co-Authors: Shaun Kernaghan, Adina R Bujold, Janet I MacinnesAbstract:The tonsil of the soft palate in pigs is a secondary lymphoid tissue that provides a first line of defense against foreign antigens entering by the mouth or nares. It has been known for a long time to be the site of colonization of important swine and zoonotic bacterial pathogens. Initially our understanding of microbes present at this site came from culture-based studies. Very recently, sequence-based approaches have been used to identify the core microbiome of the swine tonsil. Although animal to animal and herd to herd variation was detected in these studies, >90 of the organisms detected belonged to the phyla Proteobacteria and Firmicutes. Members of the family Pasteurellaceae appeared to be predominate in the tonsil; however, the relative proportions of Actinobacillus, Haemophilus, and Pasteurella varied. Members of the families Moraxellaceae, Fusobacteriaceae, Veillonellaceae, and Neisseriaceae were also seen as frequent residents of the tonsil.