The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform

William A. Paxton - One of the best experts on this subject based on the ideXlab platform.

  • Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4 + T-lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

  • Mucin 6 in seminal plasma binds dc sign and potently blocks dendritic cell mediated transfer of hiv 1 to cd4 t lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

Martijn J. Stax - One of the best experts on this subject based on the ideXlab platform.

  • Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4 + T-lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

  • Mucin 6 in seminal plasma binds dc sign and potently blocks dendritic cell mediated transfer of hiv 1 to cd4 t lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

Elisabeth Van Leeuwen - One of the best experts on this subject based on the ideXlab platform.

  • Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4 + T-lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

  • Mucin 6 in seminal plasma binds dc sign and potently blocks dendritic cell mediated transfer of hiv 1 to cd4 t lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

Dave Speijer - One of the best experts on this subject based on the ideXlab platform.

  • Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4 + T-lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

  • Mucin 6 in seminal plasma binds dc sign and potently blocks dendritic cell mediated transfer of hiv 1 to cd4 t lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

Sjoerd Repping - One of the best experts on this subject based on the ideXlab platform.

  • Mucin 6 in seminal plasma binds DC-SIGN and potently blocks dendritic cell mediated transfer of HIV-1 to CD4 + T-lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.

  • Mucin 6 in seminal plasma binds dc sign and potently blocks dendritic cell mediated transfer of hiv 1 to cd4 t lymphocytes
    Virology, 2009
    Co-Authors: Martijn J. Stax, Thijs Van Montfort, Richard R. Sprenger, Mark Melchers, Rogier W. Sanders, Elisabeth Van Leeuwen, Sjoerd Repping, Georgios Pollakis, Dave Speijer, William A. Paxton
    Abstract:

    Abstract Many viruses transmitted via the genital or oral mucosa have the potential to interact with dendritic cell-specific intercellular adhesion molecule-3 grabbing non integrin (DC-SIGN) expressed on immature dendritic cells (iDCs) that lie below the mucosal surface. These cells have been postulated to capture and disseminate human immunodeficiency virus type-1 (HIV-1) to CD4+ lymphocytes, potentially through breaches in the mucosal lining. We have previously described that BSSL (bile salt-stimulated lipase) in human milk can bind DC-SIGN and block transfer. Here we demonstrate that seminal plasma has similar DC-SIGN blocking properties as BSSL in human milk. Using comparative SDS-PAGE and Western blotting combined with mass spectrometry we identified Mucin 6 as the DC-SIGN binding component in seminal plasma. Additionally, we demonstrate that purified Mucin 6 binds DC-SIGN and successfully inhibits viral transfer. Mucin 6 in seminal plasma may therefore interfere with the sexual transmission of HIV-1 and other DC-SIGN co-opting viruses.