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Xiaofang Geng - One of the best experts on this subject based on the ideXlab platform.

  • proteomic analysis of the skin of chinese giant salamander andrias davidianus
    Journal of Proteomics, 2015
    Co-Authors: Xiaofang Geng, Bing Chen, Fuchun Zhang, Xiayan Zang, Jingyan Sun, Hong Wei, Haitao Shang, Minghui Zhou, Jing Che
    Abstract:

    Abstract The Chinese giant salamander ( Andrias davidianus ), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin has developed Mucous Gland which could secrete a large amount of mucus under the scraping and electric stimulation, and the molting is the degraded skin stratum corneum. Although several proteomic studies have focused on functional proteomes of mammalian and frog skin, the skin proteome of Chinese giant salamander has not yet been carefully studied. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. Our findings indicated that 249 proteins were identified in the skin, 155 proteins in the mucus, and 97 proteins in the molting. Furthermore, Gene Ontology (GO) analysis showed that these proteins participated in various physiological activities, including extracellular matrix organization, defense, immune response, wound healing, respiration, etc. In conclusion, the proteomic results provide new insight in the aspects of the proteomes in the skin, mucus and the molting of Chinese giant salamander. Biological significance This was the first study to examine the protein expression abundance in the skin, mucus and molting of Chinese giant salamander by a proteomics approach. Meantime, the identification of a more global proteome in normal skin may provide a basis for characterizing and comparing the skin proteomes from other amphibian species.

  • Data from proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus).
    Data in brief, 2015
    Co-Authors: Xiaofang Geng, Wei Hong, Shang Haitao, Zhou Minghui, Bing Chen, Fuchun Zhang, Xiayan Zang, Jingyan Sun, Jing Che
    Abstract:

    The Chinese giant salamander (Andrias davidianus), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin is rich in collagens, and has developed Mucous Gland which could secrete a large amount of mucus under the scraping and electric stimulation. The molting is the degraded skin stratum corneum. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. The determination of the general proteome in the skin can potentially serve as a foundation for future studies characterizing the skin proteomes from diseased salamander to provide molecular and mechanistic insights into various disease states and potential therapeutic interventions. Data presented here are also related to the research article “Proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus)” in the Journal of Proteomics [1].

Alan James - One of the best experts on this subject based on the ideXlab platform.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.

  • increased mast cells and neutrophils in submucosal Mucous Glands and mucus plugging in patients with asthma
    Thorax, 2002
    Co-Authors: N G Carroll, S Mutavdzic, Alan James
    Abstract:

    Background: Mucus plugging of the airways is invariably seen in cases of fatal asthma, mucus production is associated with asthma attacks, and the area of submucosal Glands is increased in asthma. Mediators secreted from mast cells and neutrophils can stimulate Mucous Gland secretion. A study was undertaken to count the mast cells and neutrophils in submucosal Glands and to relate cell numbers to the presence of mucus in the airway lumen. Methods: Cartilaginous airways obtained at necropsy from cases of fatal asthma (n=8), non-fatal asthma (n=8), and control cases (n=8) were examined. Contiguous transverse sections were stained for mast cell tryptase and neutrophil elastase, and with Periodic Acid Schiff solution to identify mucus. Mucous Gland area, lumen area, and the percentage of the relaxed lumen area occupied by mucus (mucus occupying ratio, MOR) were measured. Mast cells (intact and degranulated) and neutrophils per area of submucosal Gland were calculated. Results: Compared with controls, the cases of fatal asthma had increased Mucous Gland area, MOR, percentage of degranulated mast cells, and numbers of neutrophils in the submucosal Glands (p<0.05). In cases of non-fatal asthma the MOR and the numbers of mast cells and neutrophils in the submucosal Glands were increased (p<0.05). When all cases were pooled together, the MOR correlated with the total number of mast cells ( r =0.55, p=0.005) and with the number of degranulated mast cells in the submucosal Glands ( r =0.51, p=0.013), but not with the number of neutrophils ( r =0.21, p=0.121). Conclusion: These results show that Mucous Gland area, MOR, and Mucous Gland inflammation are increased in asthma and that degranulation of mast cells may contribute to secretion of mucus into the lumen in cases of fatal asthma.

Jing Che - One of the best experts on this subject based on the ideXlab platform.

  • proteomic analysis of the skin of chinese giant salamander andrias davidianus
    Journal of Proteomics, 2015
    Co-Authors: Xiaofang Geng, Bing Chen, Fuchun Zhang, Xiayan Zang, Jingyan Sun, Hong Wei, Haitao Shang, Minghui Zhou, Jing Che
    Abstract:

    Abstract The Chinese giant salamander ( Andrias davidianus ), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin has developed Mucous Gland which could secrete a large amount of mucus under the scraping and electric stimulation, and the molting is the degraded skin stratum corneum. Although several proteomic studies have focused on functional proteomes of mammalian and frog skin, the skin proteome of Chinese giant salamander has not yet been carefully studied. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. Our findings indicated that 249 proteins were identified in the skin, 155 proteins in the mucus, and 97 proteins in the molting. Furthermore, Gene Ontology (GO) analysis showed that these proteins participated in various physiological activities, including extracellular matrix organization, defense, immune response, wound healing, respiration, etc. In conclusion, the proteomic results provide new insight in the aspects of the proteomes in the skin, mucus and the molting of Chinese giant salamander. Biological significance This was the first study to examine the protein expression abundance in the skin, mucus and molting of Chinese giant salamander by a proteomics approach. Meantime, the identification of a more global proteome in normal skin may provide a basis for characterizing and comparing the skin proteomes from other amphibian species.

  • Data from proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus).
    Data in brief, 2015
    Co-Authors: Xiaofang Geng, Wei Hong, Shang Haitao, Zhou Minghui, Bing Chen, Fuchun Zhang, Xiayan Zang, Jingyan Sun, Jing Che
    Abstract:

    The Chinese giant salamander (Andrias davidianus), renowned as a living fossil, is the largest and longest-lived amphibian species in the world. Its skin is rich in collagens, and has developed Mucous Gland which could secrete a large amount of mucus under the scraping and electric stimulation. The molting is the degraded skin stratum corneum. To establish the functional skin proteome of Chinese giant salamander, two-dimensional gel electrophoresis (2DE) and mass spectrometry (MS) were applied to detect the composition and relative abundance of the proteins in the skin, mucus and molting. The determination of the general proteome in the skin can potentially serve as a foundation for future studies characterizing the skin proteomes from diseased salamander to provide molecular and mechanistic insights into various disease states and potential therapeutic interventions. Data presented here are also related to the research article “Proteomic analysis of the skin of Chinese giant salamander (Andrias davidianus)” in the Journal of Proteomics [1].

Thais Mauad - One of the best experts on this subject based on the ideXlab platform.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.

Francis H. Y. Green - One of the best experts on this subject based on the ideXlab platform.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.

  • increased myoepithelial cells of bronchial submucosal Glands in fatal asthma
    Thorax, 2010
    Co-Authors: Francis H. Y. Green, Laura J Mcphee, Alan James, Ian Mitchell, David Williams, Thais Mauad
    Abstract:

    Background: Fatal asthma is characterized by enlargement of bronchial Mucous Glands and tenacious mucus plugs in the airway lumen. Myoepithelial cells, located within the Mucous Glands, contain contractile proteins, which provide structural support to Mucous cells and actively facilitate Glandular secretion. Objectives: To determine if myoepithelial cells are increased in the bronchial submucosal Glands of fatal asthma. Design: Autopsied lungs from 12 cases of fatal asthma (FA), 12 cases of persons with asthma dying of non-respiratory causes (NFA), and 12 non-asthma control (NAC) cases were obtained through the Prairie Provinces Asthma Study. Transverse sections of segmental bronchi from three lobes were stained for mucus and smooth muscle actin and the area fractions of Mucous plugs, Mucous Glands and myoepithelial cells determined by point counting. The fine structure of the myoepithelial cells was examined by electron microscopy. Results: FA was characterized by significant increases in Mucous Gland (p=0.003), Mucous plug (p=0.004), and myoepithelial cell areas (p=0.017) compared with NAC. When the ratio of myoepithelial cell area to total Gland area was examined, there was a disproportionate and significant increase in FA compared with NAC (p=0.014). Electron microscopy of FA cases revealed hypertrophy of the myoepithelial cells with increased intracellular myofilaments. The NFA group showed changes in these features that were intermediate between the FA and NAC groups; however the differences were not significant. Conclusions: Bronchial Mucous Glands and Mucous Gland myoepithelial cell smooth muscle actin are increased in fatal asthma and may contribute to asphyxia due to Mucous plugging.