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Enrico Marinello - One of the best experts on this subject based on the ideXlab platform.

  • The protective effect of Mucuna pruriens seeds against snake venom poisoning.
    Journal of ethnopharmacology, 2009
    Co-Authors: Nget Hong Tan, Roberto Guerranti, Enrico Marinello, Shin Yee Fung, Si Mui Sim, John C. Aguiyi
    Abstract:

    Ethnopharmacological relevance: The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite. Aim of the study: To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms. Materials and methods: Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization. Results: In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested. Conclusions: The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites. © 2009 Elsevier Ireland Ltd. All rights reserved.

  • Antidiabetic oligocyclitols in seeds of Mucuna pruriens
    Phytotherapy research : PTR, 2005
    Co-Authors: Donato Donati, Lucia Raffaella Lampariello, Roberto Pagani, Roberto Guerranti, Giuliano Cinci, Enrico Marinello
    Abstract:

    Using a combination of chromatographic and NMR techniques, the presence of D-chiro-inositol and its two galacto-derivatives is demonstrated in Mucuna pruriens seeds. The quantities detected explain the well-established antiglycaemic effect of Mucuna pruriens seeds.

  • effects of Mucuna pruriens extract on activation of prothrombin by echis carinatus venom
    Journal of Ethnopharmacology, 2001
    Co-Authors: Roberto Guerranti, John C. Aguiyi, Roberto Pagani, Enza Errico, Enrico Marinello
    Abstract:

    Mucuna pruriens (L.) DC has long been used as a medicinal plant by traditional healers. The validity of the claims made for this plant has also been tested scientifically. Some of its properties are probably linked to high concentrations of dopa since it is useful in the treatment of Parkinson's disease. The antisnake properties of an extract of Mucuna pruriens' seeds (MP101UJ) in vivo were recently demonstrated and one is now investigating its biochemical mechanism. Echis carinatus venom (EV) contains a mixture of proteins that affect the coagulative cascade, causing severe bleeding and haemorrhage. Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied. An increase in procoagulant activity was found. This could explain the protective effect in vivo.

John C. Aguiyi - One of the best experts on this subject based on the ideXlab platform.

  • The protective effect of Mucuna pruriens seeds against snake venom poisoning.
    Journal of ethnopharmacology, 2009
    Co-Authors: Nget Hong Tan, Roberto Guerranti, Enrico Marinello, Shin Yee Fung, Si Mui Sim, John C. Aguiyi
    Abstract:

    Ethnopharmacological relevance: The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite. Aim of the study: To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms. Materials and methods: Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization. Results: In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested. Conclusions: The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites. © 2009 Elsevier Ireland Ltd. All rights reserved.

  • effects of Mucuna pruriens extract on activation of prothrombin by echis carinatus venom
    Journal of Ethnopharmacology, 2001
    Co-Authors: Roberto Guerranti, John C. Aguiyi, Roberto Pagani, Enza Errico, Enrico Marinello
    Abstract:

    Mucuna pruriens (L.) DC has long been used as a medicinal plant by traditional healers. The validity of the claims made for this plant has also been tested scientifically. Some of its properties are probably linked to high concentrations of dopa since it is useful in the treatment of Parkinson's disease. The antisnake properties of an extract of Mucuna pruriens' seeds (MP101UJ) in vivo were recently demonstrated and one is now investigating its biochemical mechanism. Echis carinatus venom (EV) contains a mixture of proteins that affect the coagulative cascade, causing severe bleeding and haemorrhage. Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied. An increase in procoagulant activity was found. This could explain the protective effect in vivo.

Roberto Guerranti - One of the best experts on this subject based on the ideXlab platform.

  • The protective effect of Mucuna pruriens seeds against snake venom poisoning.
    Journal of ethnopharmacology, 2009
    Co-Authors: Nget Hong Tan, Roberto Guerranti, Enrico Marinello, Shin Yee Fung, Si Mui Sim, John C. Aguiyi
    Abstract:

    Ethnopharmacological relevance: The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite. Aim of the study: To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms. Materials and methods: Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization. Results: In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested. Conclusions: The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites. © 2009 Elsevier Ireland Ltd. All rights reserved.

  • Antidiabetic oligocyclitols in seeds of Mucuna pruriens
    Phytotherapy research : PTR, 2005
    Co-Authors: Donato Donati, Lucia Raffaella Lampariello, Roberto Pagani, Roberto Guerranti, Giuliano Cinci, Enrico Marinello
    Abstract:

    Using a combination of chromatographic and NMR techniques, the presence of D-chiro-inositol and its two galacto-derivatives is demonstrated in Mucuna pruriens seeds. The quantities detected explain the well-established antiglycaemic effect of Mucuna pruriens seeds.

  • effects of Mucuna pruriens extract on activation of prothrombin by echis carinatus venom
    Journal of Ethnopharmacology, 2001
    Co-Authors: Roberto Guerranti, John C. Aguiyi, Roberto Pagani, Enza Errico, Enrico Marinello
    Abstract:

    Mucuna pruriens (L.) DC has long been used as a medicinal plant by traditional healers. The validity of the claims made for this plant has also been tested scientifically. Some of its properties are probably linked to high concentrations of dopa since it is useful in the treatment of Parkinson's disease. The antisnake properties of an extract of Mucuna pruriens' seeds (MP101UJ) in vivo were recently demonstrated and one is now investigating its biochemical mechanism. Echis carinatus venom (EV) contains a mixture of proteins that affect the coagulative cascade, causing severe bleeding and haemorrhage. Here the effect of an extract of MP101UJ in prothrombin activation by EV in vitro by clotting and chromogenic assay is studied. An increase in procoagulant activity was found. This could explain the protective effect in vivo.

Nget Hong Tan - One of the best experts on this subject based on the ideXlab platform.

  • Extracts of Cowhage (Mucuna pruriens) Seeds and Anti-Snake Venom Effects
    Nuts and Seeds in Health and Disease Prevention, 2011
    Co-Authors: Nget Hong Tan, Shin Yee Fung, Si Mui Sim
    Abstract:

    Publisher Summary This chapter highlights the anti-venom properties of cowhage seeds. It has been demonstrated in the laboratory that immunization of mice with Mucuna pruriens seed extract (MPE) elicited production of immunoglobulins that could neutralize cobra venom. Pretreatment of the animals by intraperitoneal injection of Mucuna pruriens seed extract did effectively protect the animals against the lethalities of Echis carinatus and Naja sputatrix venoms. The same treatment was moderately effective against lethalities of some other venom. It can be concluded that pretreatment with Mucuna pruriens seed extract was able to significantly inhibit the venom-induced myotoxic and cytotoxic effects and the elevation of D-dimer levels. The pretreatment also decreased the clotting time of mice injected with the venom. The protective mechanism involved an immunological mechanism. Injection of the seed extract elicited formation of antibodies that could cross-react with snake venom phospholipases A 2 , polypeptide neurotoxins, and some other venom toxins. The antibodies may be used as an alternative to commercial antivenoms. Survival studies, pharmacological experiments, and gene expression studies all suggest the involvement of a direct protective mechanism—in particular, cardioprotective action in the case of anti-cobra venom action. However, the use of raw Mucuna pruriens seeds is often accompanied by toxic symptoms, including neurotoxicity, behavioral changes, and severe vomiting.

  • The protective effect of Mucuna pruriens seeds against snake venom poisoning.
    Journal of ethnopharmacology, 2009
    Co-Authors: Nget Hong Tan, Roberto Guerranti, Enrico Marinello, Shin Yee Fung, Si Mui Sim, John C. Aguiyi
    Abstract:

    Ethnopharmacological relevance: The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite. Aim of the study: To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms. Materials and methods: Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization. Results: In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested. Conclusions: The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites. © 2009 Elsevier Ireland Ltd. All rights reserved.

Si Mui Sim - One of the best experts on this subject based on the ideXlab platform.

  • Extracts of Cowhage (Mucuna pruriens) Seeds and Anti-Snake Venom Effects
    Nuts and Seeds in Health and Disease Prevention, 2011
    Co-Authors: Nget Hong Tan, Shin Yee Fung, Si Mui Sim
    Abstract:

    Publisher Summary This chapter highlights the anti-venom properties of cowhage seeds. It has been demonstrated in the laboratory that immunization of mice with Mucuna pruriens seed extract (MPE) elicited production of immunoglobulins that could neutralize cobra venom. Pretreatment of the animals by intraperitoneal injection of Mucuna pruriens seed extract did effectively protect the animals against the lethalities of Echis carinatus and Naja sputatrix venoms. The same treatment was moderately effective against lethalities of some other venom. It can be concluded that pretreatment with Mucuna pruriens seed extract was able to significantly inhibit the venom-induced myotoxic and cytotoxic effects and the elevation of D-dimer levels. The pretreatment also decreased the clotting time of mice injected with the venom. The protective mechanism involved an immunological mechanism. Injection of the seed extract elicited formation of antibodies that could cross-react with snake venom phospholipases A 2 , polypeptide neurotoxins, and some other venom toxins. The antibodies may be used as an alternative to commercial antivenoms. Survival studies, pharmacological experiments, and gene expression studies all suggest the involvement of a direct protective mechanism—in particular, cardioprotective action in the case of anti-cobra venom action. However, the use of raw Mucuna pruriens seeds is often accompanied by toxic symptoms, including neurotoxicity, behavioral changes, and severe vomiting.

  • The protective effect of Mucuna pruriens seeds against snake venom poisoning.
    Journal of ethnopharmacology, 2009
    Co-Authors: Nget Hong Tan, Roberto Guerranti, Enrico Marinello, Shin Yee Fung, Si Mui Sim, John C. Aguiyi
    Abstract:

    Ethnopharmacological relevance: The seed, leaf and root of Mucuna pruriens have been used in traditional medicine for treatments of various diseases. In Nigeria, the seed is used as oral prophylactics for snakebite. Aim of the study: To study the protective effects of Mucuna pruriens seed extract against the lethalities of various snake venoms. Materials and methods: Rats were pre-treated with Mucuna pruriens seed extract and challenged with various snake venoms. The effectiveness of anti-Mucuna pruriens (anti-MPE) antibody to neutralize the lethalities of snake venoms was investigated by in vitro neutralization. Results: In rats, MPE pre-treatment conferred effective protection against lethality of Naja sputatrix venom and moderate protection against Calloselasma rhodostoma venom. Indirect ELISA and immunoblotting studies showed that there were extensive cross-reactions between anti-MPE IgG and venoms from many different genera of poisonous snakes, suggesting the involvement of immunological neutralization in the protective effect of MPE pre-treatment against snake venom poisoning. In vitro neutralization experiments showed that the anti-MPE antibodies effectively neutralized the lethalities of Asiatic cobra (Naja) venoms, but were not very effective against other venoms tested. Conclusions: The anti-MPE antibodies could be used in the antiserum therapy of Asiatic cobra (Naja) bites. © 2009 Elsevier Ireland Ltd. All rights reserved.