The Experts below are selected from a list of 264 Experts worldwide ranked by ideXlab platform
Patrick Prunet - One of the best experts on this subject based on the ideXlab platform.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO_4·7H_2O), over the range 0.01–1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO_4 did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO_4 reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO(4)center dot 7H(2)O), over the range 0.01-1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO(4) did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO(4) reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
Isabelle Leguen - One of the best experts on this subject based on the ideXlab platform.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO_4·7H_2O), over the range 0.01–1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO_4 did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO_4 reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO(4)center dot 7H(2)O), over the range 0.01-1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO(4) did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO(4) reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
Sandrine Peron - One of the best experts on this subject based on the ideXlab platform.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO_4·7H_2O), over the range 0.01–1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO_4 did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO_4 reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
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Effects of iron on rainbow trout gill cells in primary culture
Cell Biology and Toxicology, 2011Co-Authors: Isabelle Leguen, Sandrine Peron, Patrick PrunetAbstract:This study investigated the effects of iron in the form of iron sulphate (FeSO(4)center dot 7H(2)O), over the range 0.01-1 mM on rainbow trout primary gill cells cultured on semi-permeable membranes. The endpoints measured were cell proliferation, mucous cell numbers, area of Mucus in mucous cells, ultrastructural analysis and transepithelial resistance. Regardless of the concentration, FeSO(4) did not modify the apical surface of pavement cells (microridge) and mucous cells. However, at 1 mM, this metal reduced cell numbers, by inhibiting cell proliferation and causing cell death, and induced a decrease in transepithelial resistance. It is interesting to note that cell numbers were also reduced in the presence of 0.5 mM iron salt, although this reduction did not modify transepithelial resistance. FeSO(4) reduced mucous cell number but did not change Mucus area in mucous cells suggesting that this metal could induce a discharge of mucous cells, but Mucus secretion would be total and not partial. In conclusion, our in vitro model has allowed to study some toxic effect but also resistance of gill epithelium in presence of iron.
S Wendelaar E Bonga - One of the best experts on this subject based on the ideXlab platform.
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responses of skin mucous cells to aluminium exposure at low ph in atlantic salmon salmo salar smolts
Canadian Journal of Fisheries and Aquatic Sciences, 1997Co-Authors: Marc H G Berntssen, Frode Kroglund, Bjorn Olav Rosseland, S Wendelaar E BongaAbstract:Atlantic salmon (Salmo salar) smolts were exposed for 80 h to seven water qualities: pH 5.6 with 31 and 46 µg labile Al · L-1, pH 6.0 with 18 and 24 µg labile Al · L-1, and pH 6.2 with 12 and 18 µg labile Al · L-1 and control water at pH 6.8 and <10 µg labile Al · L-1. The three groups with the highest concentrations of labile Al (31 and 46 µg labile Al · L-1 at pH 5.6 and 24 µg labile Al · L-1 at pH 6.0) suffered high mortalities and showed a disturbance in osmoregulation and a massive secretion of Mucus, as seen from a decrease in number of skin mucous cells. Furthermore, an increase in skin mucous cells containing acidic mucosubstances was observed. The loss of plasma chloride and skin mucous cells showed a significant linear correlation (R2 = 0.68, p < 0.001). The increased secretion of Mucus on skin and gills and the increase in acidity of mucosubstances are consistent with their prior presumed defensive role in binding of Al.
Marc H G Berntssen - One of the best experts on this subject based on the ideXlab platform.
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responses of skin mucous cells to aluminium exposure at low ph in atlantic salmon salmo salar smolts
Canadian Journal of Fisheries and Aquatic Sciences, 1997Co-Authors: Marc H G Berntssen, Frode Kroglund, Bjorn Olav Rosseland, S Wendelaar E BongaAbstract:Atlantic salmon (Salmo salar) smolts were exposed for 80 h to seven water qualities: pH 5.6 with 31 and 46 µg labile Al · L-1, pH 6.0 with 18 and 24 µg labile Al · L-1, and pH 6.2 with 12 and 18 µg labile Al · L-1 and control water at pH 6.8 and <10 µg labile Al · L-1. The three groups with the highest concentrations of labile Al (31 and 46 µg labile Al · L-1 at pH 5.6 and 24 µg labile Al · L-1 at pH 6.0) suffered high mortalities and showed a disturbance in osmoregulation and a massive secretion of Mucus, as seen from a decrease in number of skin mucous cells. Furthermore, an increase in skin mucous cells containing acidic mucosubstances was observed. The loss of plasma chloride and skin mucous cells showed a significant linear correlation (R2 = 0.68, p < 0.001). The increased secretion of Mucus on skin and gills and the increase in acidity of mucosubstances are consistent with their prior presumed defensive role in binding of Al.
