The Experts below are selected from a list of 39 Experts worldwide ranked by ideXlab platform
M. Elizabeth Hartnett - One of the best experts on this subject based on the ideXlab platform.
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Mueller Cell derived VEGF164 knockdown reduces retinal neovascularisation in the rat model of retinopathy of prematurity.
Investigative Ophthalmology & Visual Science, 2014Co-Authors: Deeksha Gambhir, Yanchao Jiang, George W Smith, Z. Yang, Haibo Wang, Lori Fotheringham, M. Elizabeth HartnettAbstract:1. Lentivirus with shRNA to VEGF164 targeting Muller Cells: VEGFA and control luciferase shRNAs were created as described7. Lentivectors with a CD44 promoter and short hairpin RNA (shRNA) were used to knockdown VEGFA (VEGFA.shRNA) or VEGF164 (VEGF164.shRNA). At least two different shRNAs were created and tested for knockdown efficiency in HEK reporter Cell lines for VEGFA.shRNA and VEGF164.shRNA. rMC-1 Cells were used to test the specificity of VEGFA.shRNA, VEGF164.shRNA and luciferase control (luc.shRNA). Uninfected Cells were used as controls for luc.shRNA.
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Knockdown of Mueller Cell specific VEGF reduces retinal neovascularization in a rat model of retinopathy of prematurity (ROP)
Investigative Ophthalmology & Visual Science, 2013Co-Authors: Manabu Mccloskey, Yanchao Jiang, Z. Yang, Haibo Wang, Jeremy Strange, Tal Kafri, John G. Flannery, M. Elizabeth HartnettAbstract:in a rat model of retinopathy of prematurity Yanchao Jiang1, Manabu McCloskey1, Haibo Wang1, Zhihong Yang1, Jeremy Strange1, Tal Kafir2, John G Flannery3, Kenneth Greenberg4, Scott Hammond2, ME Hartnett1, 1Dept of Ophthalmology, John A. Moran Eye Center, University of Utah, Salt Lake City, UT 2University of North Carolina, Chapel Hill, UT 3University of California, Berkeley, CA 4Spiral Devices, LLC, Berkeley, CA
Yanchao Jiang - One of the best experts on this subject based on the ideXlab platform.
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Mueller Cell derived VEGF164 knockdown reduces retinal neovascularisation in the rat model of retinopathy of prematurity.
Investigative Ophthalmology & Visual Science, 2014Co-Authors: Deeksha Gambhir, Yanchao Jiang, George W Smith, Z. Yang, Haibo Wang, Lori Fotheringham, M. Elizabeth HartnettAbstract:1. Lentivirus with shRNA to VEGF164 targeting Muller Cells: VEGFA and control luciferase shRNAs were created as described7. Lentivectors with a CD44 promoter and short hairpin RNA (shRNA) were used to knockdown VEGFA (VEGFA.shRNA) or VEGF164 (VEGF164.shRNA). At least two different shRNAs were created and tested for knockdown efficiency in HEK reporter Cell lines for VEGFA.shRNA and VEGF164.shRNA. rMC-1 Cells were used to test the specificity of VEGFA.shRNA, VEGF164.shRNA and luciferase control (luc.shRNA). Uninfected Cells were used as controls for luc.shRNA.
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Knockdown of Mueller Cell specific VEGF reduces retinal neovascularization in a rat model of retinopathy of prematurity (ROP)
Investigative Ophthalmology & Visual Science, 2013Co-Authors: Manabu Mccloskey, Yanchao Jiang, Z. Yang, Haibo Wang, Jeremy Strange, Tal Kafri, John G. Flannery, M. Elizabeth HartnettAbstract:in a rat model of retinopathy of prematurity Yanchao Jiang1, Manabu McCloskey1, Haibo Wang1, Zhihong Yang1, Jeremy Strange1, Tal Kafir2, John G Flannery3, Kenneth Greenberg4, Scott Hammond2, ME Hartnett1, 1Dept of Ophthalmology, John A. Moran Eye Center, University of Utah, Salt Lake City, UT 2University of North Carolina, Chapel Hill, UT 3University of California, Berkeley, CA 4Spiral Devices, LLC, Berkeley, CA
Z. Yang - One of the best experts on this subject based on the ideXlab platform.
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Mueller Cell derived VEGF164 knockdown reduces retinal neovascularisation in the rat model of retinopathy of prematurity.
Investigative Ophthalmology & Visual Science, 2014Co-Authors: Deeksha Gambhir, Yanchao Jiang, George W Smith, Z. Yang, Haibo Wang, Lori Fotheringham, M. Elizabeth HartnettAbstract:1. Lentivirus with shRNA to VEGF164 targeting Muller Cells: VEGFA and control luciferase shRNAs were created as described7. Lentivectors with a CD44 promoter and short hairpin RNA (shRNA) were used to knockdown VEGFA (VEGFA.shRNA) or VEGF164 (VEGF164.shRNA). At least two different shRNAs were created and tested for knockdown efficiency in HEK reporter Cell lines for VEGFA.shRNA and VEGF164.shRNA. rMC-1 Cells were used to test the specificity of VEGFA.shRNA, VEGF164.shRNA and luciferase control (luc.shRNA). Uninfected Cells were used as controls for luc.shRNA.
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Knockdown of Mueller Cell specific VEGF reduces retinal neovascularization in a rat model of retinopathy of prematurity (ROP)
Investigative Ophthalmology & Visual Science, 2013Co-Authors: Manabu Mccloskey, Yanchao Jiang, Z. Yang, Haibo Wang, Jeremy Strange, Tal Kafri, John G. Flannery, M. Elizabeth HartnettAbstract:in a rat model of retinopathy of prematurity Yanchao Jiang1, Manabu McCloskey1, Haibo Wang1, Zhihong Yang1, Jeremy Strange1, Tal Kafir2, John G Flannery3, Kenneth Greenberg4, Scott Hammond2, ME Hartnett1, 1Dept of Ophthalmology, John A. Moran Eye Center, University of Utah, Salt Lake City, UT 2University of North Carolina, Chapel Hill, UT 3University of California, Berkeley, CA 4Spiral Devices, LLC, Berkeley, CA
Haibo Wang - One of the best experts on this subject based on the ideXlab platform.
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Mueller Cell derived VEGF164 knockdown reduces retinal neovascularisation in the rat model of retinopathy of prematurity.
Investigative Ophthalmology & Visual Science, 2014Co-Authors: Deeksha Gambhir, Yanchao Jiang, George W Smith, Z. Yang, Haibo Wang, Lori Fotheringham, M. Elizabeth HartnettAbstract:1. Lentivirus with shRNA to VEGF164 targeting Muller Cells: VEGFA and control luciferase shRNAs were created as described7. Lentivectors with a CD44 promoter and short hairpin RNA (shRNA) were used to knockdown VEGFA (VEGFA.shRNA) or VEGF164 (VEGF164.shRNA). At least two different shRNAs were created and tested for knockdown efficiency in HEK reporter Cell lines for VEGFA.shRNA and VEGF164.shRNA. rMC-1 Cells were used to test the specificity of VEGFA.shRNA, VEGF164.shRNA and luciferase control (luc.shRNA). Uninfected Cells were used as controls for luc.shRNA.
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Knockdown of Mueller Cell specific VEGF reduces retinal neovascularization in a rat model of retinopathy of prematurity (ROP)
Investigative Ophthalmology & Visual Science, 2013Co-Authors: Manabu Mccloskey, Yanchao Jiang, Z. Yang, Haibo Wang, Jeremy Strange, Tal Kafri, John G. Flannery, M. Elizabeth HartnettAbstract:in a rat model of retinopathy of prematurity Yanchao Jiang1, Manabu McCloskey1, Haibo Wang1, Zhihong Yang1, Jeremy Strange1, Tal Kafir2, John G Flannery3, Kenneth Greenberg4, Scott Hammond2, ME Hartnett1, 1Dept of Ophthalmology, John A. Moran Eye Center, University of Utah, Salt Lake City, UT 2University of North Carolina, Chapel Hill, UT 3University of California, Berkeley, CA 4Spiral Devices, LLC, Berkeley, CA
W. Richard Green - One of the best experts on this subject based on the ideXlab platform.
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Clinicopathologic Correlation of an Untreated Macular Hole and a Macular Hole Treated by Vitrectomy, Transforming Growth Factor-β2, and Gas Tamponade
American journal of ophthalmology, 1996Co-Authors: Robert H. Rosa, Bert M. Glaser, Zenaida De La Cruz, W. Richard GreenAbstract:Purpose To study the clinicopathologic features of an untreated macular hole in the right eye and a macular hole in the left eye treated by vitrectomy, application of transforming growth factor-β 2 , and gas tamponade. Methods The patient, a 73-year-old man with bilateral macular holes, was studied clinically before and after surgical treatment of the macular hole in his left eye. The patient's eyes were obtained postmortem and serially step-sectioned through the macula and optic nerve head for electron microscopy. Results Examination of 1-μm thick plastic-embedded sections through the macula of the right eye disclosed a 0.6-mm macular hole with rounded gliotic margins, a thin epiretinal membrane, and parafoveal cystic changes. Examination of 1-μm thick plastic-embedded sections through the macula of the left eye disclosed a 0.25-mm defect in the fovea, which was bridged by glial Cells. The glial Cells were continuous with a thin hypoCellular epiretinal membrane without contraction features on both sides of the defect. The ultrastructural features of the glial Cells were consistent with Mueller Cells. Conclusions Treatment of a macular hole with vitrectomy, transforming growth factor-β 2 , and gas tamponade was followed by complete closure of the macular hole by Mueller Cell proliferation.