The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Mark A Miller - One of the best experts on this subject based on the ideXlab platform.
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optochin revisited defining the optimal type of blood Agar for presumptive identification of streptococcus pneumoniae
Journal of Clinical Microbiology, 1998Co-Authors: Michael Gardam, Mark A MillerAbstract:To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia Agar, Trypticase soy Agar (TSA), and Mueller-Hinton Agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood Agar be used.
Jonathan R Carapetis - One of the best experts on this subject based on the ideXlab platform.
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Comparison of citrated human blood, citrated sheep blood, and defibrinated sheep blood Mueller-Hinton Agar preparations for antimicrobial susceptibility testing of Streptococcus pneumoniae isolates.
Journal of Clinical Microbiology, 2010Co-Authors: Catherine Satzke, A. Seduadua, E. K. Mulholland, Jonathan R Carapetis, Reginald Chandra, Fiona M. RussellAbstract:The use of Mueller-Hinton Agar supplemented with citrated human or citrated sheep blood was compared with the use of routinely used Mueller-Hinton Agar supplemented with defibrinated sheep blood for antimicrobial susceptibility testing of Streptococcus pneumoniae. The alternate supplements were found to be unsatisfactory, particularly for testing resistant isolates, and therefore are not recommended.
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As a bacterial culture medium, citrated sheep blood Agar is a practical alternative to citrated human blood Agar in laboratories of developing countries
Journal of Clinical Microbiology, 2006Co-Authors: F. M. Russell, S. S.n. Biribo, F. Oppedisano, A. Seduadua, Gopinath Selvaraj, E. K. Mulholland, S Warren, Jonathan R CarapetisAbstract:Human blood Agar (HuBA) is widely used in developing countries for the isolation of bacteria from clinical specimens. This study compared citrated sheep blood Agar (CSBA) and HuBA with defibrinated horse blood Agar and defibrinated sheep blood Agar (DSBA) for the isolation and antibiotic susceptibility testing of reference and clinical strains of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus. Reference and clinical strains of all organisms were diluted in brain heart infusion and a clinical specimen of cerebrospinal fluid and cultured on all Agars. Viable counts, colony morphology, and colony size were recorded. Susceptibility testing for S. pneumoniae and S. pyogenes was performed on defibrinated sheep blood Mueller-Hinton Agar, citrated sheep blood Mueller-Hinton Agar (CSB MHA), and human blood Mueller-Hinton Agar plates. For all organisms, the colony numbers were similar on all Agars. Substantially smaller colony sizes and absent or minimal hemolysis were noted on HuBA for all organisms. Antibiotic susceptibility results for S. pneumoniae were similar for the two sheep blood Agars; however, larger zone sizes were displayed on HuBA, and quality control for the reference strain failed on HuBA. For S. pyogenes, larger zone sizes were demonstrated on HuBA and CSBA than on DSBA. Poor hemolysis made interpretation of the zone sizes difficult on HuBA. CSBA is an acceptable alternative for the isolation of these organisms. The characteristic morphology is not evident, and hemolysis is poor on HuBA; and so HuBA is not recommended for use for the isolation or the susceptibility testing of any of these organisms. CSB MHA may be suitable for use for the susceptibility testing of S. pneumoniae.
Michael Gardam - One of the best experts on this subject based on the ideXlab platform.
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optochin revisited defining the optimal type of blood Agar for presumptive identification of streptococcus pneumoniae
Journal of Clinical Microbiology, 1998Co-Authors: Michael Gardam, Mark A MillerAbstract:To determine the optimal media for optochin susceptibility testing of Streptococcus pneumoniae, we measured inhibition zones for 72 S. pneumoniae and 22 Streptococcus viridans isolates on three blood-containing media. Because 15.3, 0, and 22.2% of S. pneumoniae organisms were misidentified on Columbia Agar, Trypticase soy Agar (TSA), and Mueller-Hinton Agar, respectively, each containing sheep blood, we recommend that TSA-sheep blood Agar be used.
Birgit Leonhard - One of the best experts on this subject based on the ideXlab platform.
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Agar disk diffusion (Bauer-Kirby) tests with various fastidious and nonfastidious reference (ATCC) strains: comparison of several Agar media.
Chemotherapy, 2009Co-Authors: Walter H. Traub, Birgit LeonhardAbstract:Several Agar media (Mueller-Hinton Agar, MHA; diagnostic sensitivity test Agar, DSTA; Schaedler Agar, SchA; Todd-Hewitt Agar with added yeast extract, THYA; Wilkins-Chalgren Agar, WCA) were compared u
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Antagonism of Teicoplanin and Vancomycin Activity by Chocolatized Mueller-Hinton Agar
Chemotherapy, 1996Co-Authors: Walter H. Traub, Birgit LeonhardAbstract:Chocolatized (80°C, 13min) Mueller-Hinton Agar antagonized the inhibitory activities of teicoplanin and vancomycin against reference strains of Streptococcus pyogenes, Streptococcus agalactiae, Streptococcus pneumoniae, Staphylococcus aureus, and Enterococcus faecalis. This antagonism was due to heat-exposed sheep erythrocytes, sheep hemoglobin, and the supernatant fluid from lysed sheep erythrocytes, but not to sheep serum. Neither water-soluble cholesterol, bovine albumin, bovine serum, hematin, hemin nor egg yolk suspension antagonized teicoplanin and vancomycin.
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Antibiotic Susceptibility of Clinical Isolates of Streptococcus pneumoniae
Chemotherapy, 1996Co-Authors: Walter H. Traub, Birgit LeonhardAbstract:Ninety-three representative, recent clinical isolates of Streptococcus pneumoniae were examined for susceptibility to 9 antimicrobial drugs utilizing Mueller-Hinton Agar (MHA) enric
E. K. Mulholland - One of the best experts on this subject based on the ideXlab platform.
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Comparison of citrated human blood, citrated sheep blood, and defibrinated sheep blood Mueller-Hinton Agar preparations for antimicrobial susceptibility testing of Streptococcus pneumoniae isolates.
Journal of Clinical Microbiology, 2010Co-Authors: Catherine Satzke, A. Seduadua, E. K. Mulholland, Jonathan R Carapetis, Reginald Chandra, Fiona M. RussellAbstract:The use of Mueller-Hinton Agar supplemented with citrated human or citrated sheep blood was compared with the use of routinely used Mueller-Hinton Agar supplemented with defibrinated sheep blood for antimicrobial susceptibility testing of Streptococcus pneumoniae. The alternate supplements were found to be unsatisfactory, particularly for testing resistant isolates, and therefore are not recommended.
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As a bacterial culture medium, citrated sheep blood Agar is a practical alternative to citrated human blood Agar in laboratories of developing countries
Journal of Clinical Microbiology, 2006Co-Authors: F. M. Russell, S. S.n. Biribo, F. Oppedisano, A. Seduadua, Gopinath Selvaraj, E. K. Mulholland, S Warren, Jonathan R CarapetisAbstract:Human blood Agar (HuBA) is widely used in developing countries for the isolation of bacteria from clinical specimens. This study compared citrated sheep blood Agar (CSBA) and HuBA with defibrinated horse blood Agar and defibrinated sheep blood Agar (DSBA) for the isolation and antibiotic susceptibility testing of reference and clinical strains of Streptococcus pneumoniae, Streptococcus pyogenes, and Staphylococcus aureus. Reference and clinical strains of all organisms were diluted in brain heart infusion and a clinical specimen of cerebrospinal fluid and cultured on all Agars. Viable counts, colony morphology, and colony size were recorded. Susceptibility testing for S. pneumoniae and S. pyogenes was performed on defibrinated sheep blood Mueller-Hinton Agar, citrated sheep blood Mueller-Hinton Agar (CSB MHA), and human blood Mueller-Hinton Agar plates. For all organisms, the colony numbers were similar on all Agars. Substantially smaller colony sizes and absent or minimal hemolysis were noted on HuBA for all organisms. Antibiotic susceptibility results for S. pneumoniae were similar for the two sheep blood Agars; however, larger zone sizes were displayed on HuBA, and quality control for the reference strain failed on HuBA. For S. pyogenes, larger zone sizes were demonstrated on HuBA and CSBA than on DSBA. Poor hemolysis made interpretation of the zone sizes difficult on HuBA. CSBA is an acceptable alternative for the isolation of these organisms. The characteristic morphology is not evident, and hemolysis is poor on HuBA; and so HuBA is not recommended for use for the isolation or the susceptibility testing of any of these organisms. CSB MHA may be suitable for use for the susceptibility testing of S. pneumoniae.