The Experts below are selected from a list of 7752 Experts worldwide ranked by ideXlab platform
Tatiana P Ugarova - One of the best experts on this subject based on the ideXlab platform.
-
control of integrin αiibβ3 outside in signaling and platelet adhesion by sensing the physical properties of fibrin ogen substrates
Biochemistry, 2010Co-Authors: Nataly P Podolnikova, Ivan S Yermolenko, Alexander Fuhrmann, Valeryi K Lishko, Sergei Magonov, Benjamin P Bowen, Joerg Enderlein, Andriy V Podolnikov, Robert Ros, Tatiana P UgarovaAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in an aggregated Multilayered Material characterized by low adhesion forces. In contrast, immobiliz...
-
physical properties of fibrinogen substrates control integrin mediated cell adhesion
Biophysical Journal, 2010Co-Authors: Ivan S Yermolenko, Alexander Fuhrmann, Tatiana P Ugarova, Robert RosAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Recently, we have shown that binding of fibrinogen to the surface of fibrin gel prevents integrin αMβ2-mediated leukocyte adhesion by creating an anti-adhesive layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive.To gain an understanding of the mechanism underlying differential cell adhesion, we extended the cell adhesion assays to platelets carrying integrin αIIbβ3. The results showed a similar behavior indicating that the process is independent of the type of integrins. In order to quantify the adhesion forces, we applied single cell force spectroscopy (SCFS). In this assay, a single cell is attached to a tipless cantilever of an atomic force microscope (AFM) and force-distance curves for different surfaces are acquired. For cells carrying αMβ2-integrins we found significant lower adhesion forces for high- compared to low-density fibrinogen substrates.Furthermore, we analyzed the adhesive behavior of fibrinogen surfaces using force spectroscopy with a silicon nitride AFM tip. These experiments, unrelated to the cells and integrins, show similar behaviors as the cell adhesion assays. AFM images of the different substrates indicate that fibrinogen deposition at high density results in an aggregated Multilayered Material characterized by low adhesion forces. However, low-density fibrinogen produces a single layer in which molecules are directly attached to the solid surface resulting in higher adhesion forces.The data suggest that deposition of a Multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties of surfaces resulting in reduced force generation with implications for hemostasis and bioMaterial applications.
Robert Ros - One of the best experts on this subject based on the ideXlab platform.
-
control of integrin αiibβ3 outside in signaling and platelet adhesion by sensing the physical properties of fibrin ogen substrates
Biochemistry, 2010Co-Authors: Nataly P Podolnikova, Ivan S Yermolenko, Alexander Fuhrmann, Valeryi K Lishko, Sergei Magonov, Benjamin P Bowen, Joerg Enderlein, Andriy V Podolnikov, Robert Ros, Tatiana P UgarovaAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in an aggregated Multilayered Material characterized by low adhesion forces. In contrast, immobiliz...
-
physical properties of fibrinogen substrates control integrin mediated cell adhesion
Biophysical Journal, 2010Co-Authors: Ivan S Yermolenko, Alexander Fuhrmann, Tatiana P Ugarova, Robert RosAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Recently, we have shown that binding of fibrinogen to the surface of fibrin gel prevents integrin αMβ2-mediated leukocyte adhesion by creating an anti-adhesive layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive.To gain an understanding of the mechanism underlying differential cell adhesion, we extended the cell adhesion assays to platelets carrying integrin αIIbβ3. The results showed a similar behavior indicating that the process is independent of the type of integrins. In order to quantify the adhesion forces, we applied single cell force spectroscopy (SCFS). In this assay, a single cell is attached to a tipless cantilever of an atomic force microscope (AFM) and force-distance curves for different surfaces are acquired. For cells carrying αMβ2-integrins we found significant lower adhesion forces for high- compared to low-density fibrinogen substrates.Furthermore, we analyzed the adhesive behavior of fibrinogen surfaces using force spectroscopy with a silicon nitride AFM tip. These experiments, unrelated to the cells and integrins, show similar behaviors as the cell adhesion assays. AFM images of the different substrates indicate that fibrinogen deposition at high density results in an aggregated Multilayered Material characterized by low adhesion forces. However, low-density fibrinogen produces a single layer in which molecules are directly attached to the solid surface resulting in higher adhesion forces.The data suggest that deposition of a Multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties of surfaces resulting in reduced force generation with implications for hemostasis and bioMaterial applications.
Ivan S Yermolenko - One of the best experts on this subject based on the ideXlab platform.
-
control of integrin αiibβ3 outside in signaling and platelet adhesion by sensing the physical properties of fibrin ogen substrates
Biochemistry, 2010Co-Authors: Nataly P Podolnikova, Ivan S Yermolenko, Alexander Fuhrmann, Valeryi K Lishko, Sergei Magonov, Benjamin P Bowen, Joerg Enderlein, Andriy V Podolnikov, Robert Ros, Tatiana P UgarovaAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in an aggregated Multilayered Material characterized by low adhesion forces. In contrast, immobiliz...
-
physical properties of fibrinogen substrates control integrin mediated cell adhesion
Biophysical Journal, 2010Co-Authors: Ivan S Yermolenko, Alexander Fuhrmann, Tatiana P Ugarova, Robert RosAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Recently, we have shown that binding of fibrinogen to the surface of fibrin gel prevents integrin αMβ2-mediated leukocyte adhesion by creating an anti-adhesive layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive.To gain an understanding of the mechanism underlying differential cell adhesion, we extended the cell adhesion assays to platelets carrying integrin αIIbβ3. The results showed a similar behavior indicating that the process is independent of the type of integrins. In order to quantify the adhesion forces, we applied single cell force spectroscopy (SCFS). In this assay, a single cell is attached to a tipless cantilever of an atomic force microscope (AFM) and force-distance curves for different surfaces are acquired. For cells carrying αMβ2-integrins we found significant lower adhesion forces for high- compared to low-density fibrinogen substrates.Furthermore, we analyzed the adhesive behavior of fibrinogen surfaces using force spectroscopy with a silicon nitride AFM tip. These experiments, unrelated to the cells and integrins, show similar behaviors as the cell adhesion assays. AFM images of the different substrates indicate that fibrinogen deposition at high density results in an aggregated Multilayered Material characterized by low adhesion forces. However, low-density fibrinogen produces a single layer in which molecules are directly attached to the solid surface resulting in higher adhesion forces.The data suggest that deposition of a Multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties of surfaces resulting in reduced force generation with implications for hemostasis and bioMaterial applications.
Alexander Fuhrmann - One of the best experts on this subject based on the ideXlab platform.
-
control of integrin αiibβ3 outside in signaling and platelet adhesion by sensing the physical properties of fibrin ogen substrates
Biochemistry, 2010Co-Authors: Nataly P Podolnikova, Ivan S Yermolenko, Alexander Fuhrmann, Valeryi K Lishko, Sergei Magonov, Benjamin P Bowen, Joerg Enderlein, Andriy V Podolnikov, Robert Ros, Tatiana P UgarovaAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Fibrin and fibrinogen, the principal components of hemostatic and pathological thrombi, may represent biologically relevant substrates whose variable physical properties control adhesion of leukocytes and platelets. In our previous work, we have shown that binding of fibrinogen to the surface of fibrin clot prevents cell adhesion by creating an antiadhesive fibrinogen layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive. To explore the mechanism underlying differential cell adhesion, we examined the structural and physical properties of surfaces prepared by deposition of various concentrations of fibrinogen using atomic force microscopy and force spectroscopy. Fibrinogen deposition at high density resulted in an aggregated Multilayered Material characterized by low adhesion forces. In contrast, immobiliz...
-
physical properties of fibrinogen substrates control integrin mediated cell adhesion
Biophysical Journal, 2010Co-Authors: Ivan S Yermolenko, Alexander Fuhrmann, Tatiana P Ugarova, Robert RosAbstract:The physical properties of substrates are known to control cell adhesion via integrin-mediated signaling. Recently, we have shown that binding of fibrinogen to the surface of fibrin gel prevents integrin αMβ2-mediated leukocyte adhesion by creating an anti-adhesive layer. Furthermore, fibrinogen immobilized on various surfaces at high density supports weak cell adhesion whereas at low density it is highly adhesive.To gain an understanding of the mechanism underlying differential cell adhesion, we extended the cell adhesion assays to platelets carrying integrin αIIbβ3. The results showed a similar behavior indicating that the process is independent of the type of integrins. In order to quantify the adhesion forces, we applied single cell force spectroscopy (SCFS). In this assay, a single cell is attached to a tipless cantilever of an atomic force microscope (AFM) and force-distance curves for different surfaces are acquired. For cells carrying αMβ2-integrins we found significant lower adhesion forces for high- compared to low-density fibrinogen substrates.Furthermore, we analyzed the adhesive behavior of fibrinogen surfaces using force spectroscopy with a silicon nitride AFM tip. These experiments, unrelated to the cells and integrins, show similar behaviors as the cell adhesion assays. AFM images of the different substrates indicate that fibrinogen deposition at high density results in an aggregated Multilayered Material characterized by low adhesion forces. However, low-density fibrinogen produces a single layer in which molecules are directly attached to the solid surface resulting in higher adhesion forces.The data suggest that deposition of a Multilayered fibrinogen matrix prevents stable cell adhesion by modifying the physical properties of surfaces resulting in reduced force generation with implications for hemostasis and bioMaterial applications.
Tanmay Basak - One of the best experts on this subject based on the ideXlab platform.
-
analysis of microwave propagation for Multilayered Material processing lambert s law versus exact solution
Industrial & Engineering Chemistry Research, 2004Co-Authors: Tanmay BasakAbstract:Microwave heating of a Multilayered system has wide applications in thawing, drying, hyperthermia treatment, and many more. A typical Multilayered system can be viewed as a combination of low and high dielectric Materials. The propagation of microwaves within a Multilayered system can be represented either by exponential Lambert's law or by an exact solution of Maxwell's equation. The exact solution for microwave propagation is based on both the transmitted and reflected waves, whereas Lambert's law is based purely on transmission. Lambert's law is a good approximation for a semi-infinite sample, and the validity of Lambert's law for a Multilayered assembly is limited to orientation of various dielectrics. Two specific cases for a Multilayered sample are considered, and the detailed scattering effects during microwave propagation have been analyzed via Lambert's law and the exact solution.