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Dianping Tang - One of the best experts on this subject based on the ideXlab platform.

  • paper electrode based flexible pressure sensor for point of care immunoassay with digital Multimeter
    Analytical Chemistry, 2019
    Co-Authors: Yu Tang, Guoneng Cai, Dianping Tang
    Abstract:

    A novel paper electrode-based flexible pressure sensor modified with multiwalled carbon nanotubes was designed for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA) with digital Multimeter readout. The portable POC testing device consisted of flexible pressure sensor equipped with a paper electrode and connected through syringe tubing to a single-break microplate. The immunoreaction was initially carried out on the microplate with a sandwich-type assay format using platinum nanozyme-labeled secondary antibody for the gas generation. Upon addition of hydrogen peroxide (H2O2), platinum nanozyme (catalase-like mimic) reduced it into hydrogen oxide and oxygen (O2). The overflowing oxygen gas increased the pressure of the multiwalled carbon nanotube-functionalized paper electrode in a homemade pressure-tight system, and the increased pressure could be readily monitored using the paper electrode-based flexible pressure-tight sensor with a digital Multimeter readout. The detectable signal mainly ...

  • a new enzyme immunoassay for alpha fetoprotein in a separate setup coupling an aluminium prussian blue based self powered electrochromic display with a digital Multimeter readout
    Analyst, 2018
    Co-Authors: Guoneng Cai, Rongrong Ren, Dianping Tang
    Abstract:

    A new enzyme immunosensing platform was designed for the simple, rapid and sensitive detection of disease-related biomarkers (alpha-fetoprotein, AFP, was used as a model in this case), coupling an aluminium (Al)/Prussian blue-based self-powered electrochromic display with a digital Multimeter readout.

  • semiautomated support photoelectrochemical immunosensing platform for portable and high throughput immunoassay based on au nanocrystal decorated specific crystal facets bivo4 photoanode
    Analytical Chemistry, 2016
    Co-Authors: Jian Shu, Guoneng Cai, Zhenli Qiu, Zhenzhen Lin, Huanghao Yang, Dianping Tang
    Abstract:

    Photoelectrochemical (PEC) measurement has been developed rapidly for bioanalysis in recent years. However, the actual application for most existed PEC bioanalytical systems is still a challenge because the perfect solutions for sensing surface design, high-throughput detection, and portability are lacked. To successfully overcome these limitations and realize accurate, continuous screening and assessing on prognostic indicator of early stage cancer on the spot, an innovative and portable semiautomated support power-free photoelectrochemical (SP-PEC) immunosensing platform consisted with a miniature semiautomatic injection system and digital Multimeter (DMM) readout is designed (prostate specific antigen, PSA, was used as the proof-of-concept analyte). Decahedral BiVO4 that decorated with Au nanocrystal on {010} facets (Au-BiVO4) by photodeposition is used as the photoanode materials to produce photocurrent signal under irradiation of micro laser light (5.0 w, λ ≥ 380 nm). The monoclonal anti-PSA capture ...

  • enzymatic oxydate triggered self illuminated photoelectrochemical sensing platform for portable immunoassay using digital Multimeter
    Analytical Chemistry, 2016
    Co-Authors: Jian Shu, Zhenli Qiu, Qian Zhou, Youxiu Lin, Dianping Tang
    Abstract:

    Herein a novel split-type photoelectrochemical (PEC) immunosensing platform was designed for sensitive detection of low-abundance biomarkers (prostate-specific antigen, PSA, used in this case) by coupling a peroxyoxalate chemiluminescence (PO-CL) self-illuminated system with digital Multimeter (DMM) readout. The PEC detection device consisted of a capacitor/DMM-joined electronic circuit and a PO-CL-based self-illuminated cell. Initially, reduced graphene oxide-doped BiVO4 (BiVO4-rGO) photovoltaic materials with good photoelectric properties was integrated into the capacitor/DMM-joined circuit for photocurrent generation in the presence of hydrogen peroxide (H2O2, as the hole-trapping reagent). A sandwich-type immunoreaction with target PSA was carried out in capture antibody-coated microplates by using glucose oxidase/detection antibody-conjugating gold nanoparticle (pAb2-AuNP-GOx). Accompanying the sandwiched immunocomplex, the labeled GOx could oxidize glucose to produce H2O2. The as-generated H2O2 coul...

  • digital Multimeter based immunosensing strategy for sensitive monitoring of biomarker by coupling an external capacitor with an enzymatic catalysis
    Biosensors and Bioelectronics, 2014
    Co-Authors: Dianping Tang, Bing Zhang, Bingqian Liu, Guonan Chen
    Abstract:

    Abstract A new digital Multimeter (DMM)-based immunosensing system was designed for quantitative monitoring of biomarker (prostate-specific antigen, PSA used in this case) by coupling with an external capacitor and an enzymatic catalytic reaction. The system consisted of a salt bridge-linked reaction cell and a capacitor/DMM-joined electronic circuit. A sandwich-type immunoreaction with target PSA between the immobilized primary antibody and glucose oxidase (GO x )-labeled detection antibody was initially carried out in one of the two half-cells. Accompanying the sandwiched immunocomplex, the conjugated GO x could catalyze the oxidation of glucose, simultaneously resulting in the conversion of [Fe(CN) 6 ] 3− to [Fe(CN) 6 ] 4− . The difference in the concentrations of [Fe(CN) 6 ] 3− /[Fe(CN) 6 ] 4− in two half-cells automatically produced a voltage that was utilized to charge an external capacitor. With the closing circuit switch, the capacitor discharged through the DMM, which could provide a high instantaneous current. Under the optimal conditions, the resulting currents was indirectly proportional to the concentration of target PSA in the dynamic range of 0.05–7 ng mL −1 with a detection limit (LOD) of 6 pg mL −1 . The reproducibility, precision, and selectivity were acceptable. In addition, the methodology was validated by analyzing 12 clinical serum specimens, receiving a good accordance with the referenced values for the detection of PSA.

Roberto Manduchi - One of the best experts on this subject based on the ideXlab platform.

  • characterizing energy consumption in a visual sensor network testbed
    Testbeds and Research Infrastructures for the DEvelopment of NeTworks and COMmunities, 2006
    Co-Authors: Cintia B Margi, Vladislav Petkov, Katia Obraczka, Roberto Manduchi
    Abstract:

    In this work we characterize the energy consumption of a visual sensor network testbed. Each node in the testbed consists of a "single-board computer", namely Crossbow's Stargate, equipped with a wireless network card and a Webcam. We assess energy consumption of activities representative of the target application (e.g., perimeter surveillance) using a benchmark that runs (individual and combinations of) "basic" tasks such as processing, flash memory access, image acquisition, and communication over the network. In our characterization, we consider the various hardware states the system switches through as it executes these benchmarks, e.g., different radio modes (sleep, idle, transmission, reception), and Webcam modes (off, on, and acquiring image). We report both steady-state and transient energy consumption behavior obtained by direct measurements of current with a digital Multimeter. We validate our measurements against results obtained using the Stargate's on-board energy consumption measuring capabilities.

Guoneng Cai - One of the best experts on this subject based on the ideXlab platform.

  • paper electrode based flexible pressure sensor for point of care immunoassay with digital Multimeter
    Analytical Chemistry, 2019
    Co-Authors: Yu Tang, Guoneng Cai, Dianping Tang
    Abstract:

    A novel paper electrode-based flexible pressure sensor modified with multiwalled carbon nanotubes was designed for point-of-care (POC) immunoassay of carcinoembryonic antigen (CEA) with digital Multimeter readout. The portable POC testing device consisted of flexible pressure sensor equipped with a paper electrode and connected through syringe tubing to a single-break microplate. The immunoreaction was initially carried out on the microplate with a sandwich-type assay format using platinum nanozyme-labeled secondary antibody for the gas generation. Upon addition of hydrogen peroxide (H2O2), platinum nanozyme (catalase-like mimic) reduced it into hydrogen oxide and oxygen (O2). The overflowing oxygen gas increased the pressure of the multiwalled carbon nanotube-functionalized paper electrode in a homemade pressure-tight system, and the increased pressure could be readily monitored using the paper electrode-based flexible pressure-tight sensor with a digital Multimeter readout. The detectable signal mainly ...

  • a new enzyme immunoassay for alpha fetoprotein in a separate setup coupling an aluminium prussian blue based self powered electrochromic display with a digital Multimeter readout
    Analyst, 2018
    Co-Authors: Guoneng Cai, Rongrong Ren, Dianping Tang
    Abstract:

    A new enzyme immunosensing platform was designed for the simple, rapid and sensitive detection of disease-related biomarkers (alpha-fetoprotein, AFP, was used as a model in this case), coupling an aluminium (Al)/Prussian blue-based self-powered electrochromic display with a digital Multimeter readout.

  • semiautomated support photoelectrochemical immunosensing platform for portable and high throughput immunoassay based on au nanocrystal decorated specific crystal facets bivo4 photoanode
    Analytical Chemistry, 2016
    Co-Authors: Jian Shu, Guoneng Cai, Zhenli Qiu, Zhenzhen Lin, Huanghao Yang, Dianping Tang
    Abstract:

    Photoelectrochemical (PEC) measurement has been developed rapidly for bioanalysis in recent years. However, the actual application for most existed PEC bioanalytical systems is still a challenge because the perfect solutions for sensing surface design, high-throughput detection, and portability are lacked. To successfully overcome these limitations and realize accurate, continuous screening and assessing on prognostic indicator of early stage cancer on the spot, an innovative and portable semiautomated support power-free photoelectrochemical (SP-PEC) immunosensing platform consisted with a miniature semiautomatic injection system and digital Multimeter (DMM) readout is designed (prostate specific antigen, PSA, was used as the proof-of-concept analyte). Decahedral BiVO4 that decorated with Au nanocrystal on {010} facets (Au-BiVO4) by photodeposition is used as the photoanode materials to produce photocurrent signal under irradiation of micro laser light (5.0 w, λ ≥ 380 nm). The monoclonal anti-PSA capture ...

Cintia B Margi - One of the best experts on this subject based on the ideXlab platform.

  • characterizing energy consumption in a visual sensor network testbed
    Testbeds and Research Infrastructures for the DEvelopment of NeTworks and COMmunities, 2006
    Co-Authors: Cintia B Margi, Vladislav Petkov, Katia Obraczka, Roberto Manduchi
    Abstract:

    In this work we characterize the energy consumption of a visual sensor network testbed. Each node in the testbed consists of a "single-board computer", namely Crossbow's Stargate, equipped with a wireless network card and a Webcam. We assess energy consumption of activities representative of the target application (e.g., perimeter surveillance) using a benchmark that runs (individual and combinations of) "basic" tasks such as processing, flash memory access, image acquisition, and communication over the network. In our characterization, we consider the various hardware states the system switches through as it executes these benchmarks, e.g., different radio modes (sleep, idle, transmission, reception), and Webcam modes (off, on, and acquiring image). We report both steady-state and transient energy consumption behavior obtained by direct measurements of current with a digital Multimeter. We validate our measurements against results obtained using the Stargate's on-board energy consumption measuring capabilities.

Jingdong Zhang - One of the best experts on this subject based on the ideXlab platform.