The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Twana Ahmed Mustafa - One of the best experts on this subject based on the ideXlab platform.
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cytotoxic and cytogenetic effects of aqueous and methanol crude extracts ofnicotiana tabacum on rhabdoMyosarcoma rd and l20b cell lines in vitro
European Journal of Experimental Biology, 2014Co-Authors: Asaad A B Alasady, Nadya Y Ahmed, Twana Ahmed MustafaAbstract:The present study was carried out to evaluate the c ytotoxicity of aqueous and methanol crude extracts of locally Nicotiana tabacum leaves on two cell lines, Rhabdom yosarcoma (RD) tumor cell line, and Murine fibrobla st (L20B) cell line in vitro. The cytogenetic effects of methanol crude extract was studied also on both cell l ines by evaluation the mitotic index (M.I) after estimating cytotoxic concentration 50% (CC50%) value for extract. The results of cytotoxic effect of aqueous and methanol crude ext racts of N. tabacum revealed that both extracts ha d a cytotoxic effect against both RD and L20B cell lines, in whic h a significant decrease in proliferation of RD tum or cell line was observed at 78.125 to 10000µg/ml of methanol crude extracts after 72hrs treatm ent. Aqueous extract showed cytotoxic effect on both RD and L20B cell lines at all concentrations also after 72hrs treatment. The CC50 values were 2100 µg/ml for RD and 2150µg/ml for L20B cell lines after 72hrs exposure with methanol extract. The cytogenetic effects of methanolic crude extract of leaves of N. tabacum revealed a significant decreas e in M.I in both cell lines.
Marten Ferno - One of the best experts on this subject based on the ideXlab platform.
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myofibromatosis like hemangiopericytoma metastasizing as differentiated vascular smooth muscle and Myosarcoma myopericytes as a subset of myofibroblasts
The American Journal of Surgical Pathology, 1992Co-Authors: Michael Dictor, Ake Elner, Torsten Andersson, Marten FernoAbstract:A thyroid hemangiopericytoma that was resected in a 5-year-old boy recurred insidiously in the larynx 8 years later. Marked cicatricial mucosal inflammation prevented a definitive pathologic diagnosis of recurrence until a nodule grew to obstruct the airway 15 years after initial surgery. After excision of the nodule, a larger sarcomatous metastasis was discovered in the upper esophagus and resected, but the patient eventually succumbed to widespread disease at the age of 20 years. The original tumor contained atypical pericytes and bundles of hyalinizing smooth muscle abutting on “staghorn vessels,” a pattern similar to infantile myofibromatosis. Desmin immunostaining was negative in the pericytes but positive in smooth-muscle cells dispersed singly as well as in bundles. Both elements reacted strongly for vimentin and the α-isoform of actin (α-SMA) found in normal smooth muscle and pericytes. A third cell type showing dendritic processes and immunoreactivity for all three antigens was interpreted as a myopericyte. Spindled cells in multiple subsequent mucosal biopsy specimens stained retrospectively also positive for these antigens. Large bundles of vascular smooth muscle surrounded by radiating myocytes characterized the occluding laryngeal nodule. In the esophageal metastasis, which showed no histologic features typical of hemangiopericytoma, numerous mitotically active, small, vimentin+, desmin+, α-SMA+ cells often maintained shortened processes and tended to form nodular aggregates about capillaries. Single rows of pericytes accreted to endothelial tubes. Ultrastructurally, some cells contained myofilaments and irregular dense material or showed rare cell junctions and variable investment by a basal lamina. This case vividly illustrates the potential for tumors composed predominantly of pericytes to differentiate toward smooth muscle and also identifies myopericytes as vimentin+, desmin+, α-actin+ dendritic cells. It suggests further that some tumors with a hemangiopericytomatous pattern and a presumed myofibroblastic component, such as infantile myofibromatosis, may reflect a benign disturbance in the differentiation of smooth muscle and pericytes, giving rise to myopericytes rather than true myofibroblasts.
Asaad A B Alasady - One of the best experts on this subject based on the ideXlab platform.
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cytotoxic and cytogenetic effects of aqueous and methanol crude extracts ofnicotiana tabacum on rhabdoMyosarcoma rd and l20b cell lines in vitro
European Journal of Experimental Biology, 2014Co-Authors: Asaad A B Alasady, Nadya Y Ahmed, Twana Ahmed MustafaAbstract:The present study was carried out to evaluate the c ytotoxicity of aqueous and methanol crude extracts of locally Nicotiana tabacum leaves on two cell lines, Rhabdom yosarcoma (RD) tumor cell line, and Murine fibrobla st (L20B) cell line in vitro. The cytogenetic effects of methanol crude extract was studied also on both cell l ines by evaluation the mitotic index (M.I) after estimating cytotoxic concentration 50% (CC50%) value for extract. The results of cytotoxic effect of aqueous and methanol crude ext racts of N. tabacum revealed that both extracts ha d a cytotoxic effect against both RD and L20B cell lines, in whic h a significant decrease in proliferation of RD tum or cell line was observed at 78.125 to 10000µg/ml of methanol crude extracts after 72hrs treatm ent. Aqueous extract showed cytotoxic effect on both RD and L20B cell lines at all concentrations also after 72hrs treatment. The CC50 values were 2100 µg/ml for RD and 2150µg/ml for L20B cell lines after 72hrs exposure with methanol extract. The cytogenetic effects of methanolic crude extract of leaves of N. tabacum revealed a significant decreas e in M.I in both cell lines.
Marcel Joniau - One of the best experts on this subject based on the ideXlab platform.
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polar agents with differentiation inducing capacity potentiate tumor necrosis factor mediated cytotoxicity in human myeloid cell lines
Journal of Leukocyte Biology, 1995Co-Authors: Stany Depraetere, Bart Vanhaesebroeck, Walter Fiers, Jean Willems, Marcel JoniauAbstract:Cotreatment or pretreatment of several human myeloid cell lines (KG1, HL60, U937, THP1) with the differentiation inducer DMSO was found to potentiate the antiproliferative and cytotoxic effects of TNF. In addition, TNF-resistant monocytic cell lines could be sensitized to TNF cytotoxicity by DMSO treatment. Other highly polar molecules, known to be potent differentiation inducers, showed similar effects to those of DMSO. The potentiating effect of DMSO was related neither to an up-regulation of TNF receptor expression nor to an alteration in the rate of TNF internalization and degradation. We present evidence that the TNF activities are p55 TNF receptor-mediated and are not due to insertion of TNF into lipid bilayers, an effect that could be susceptible to DMSO, as this component has been described to modify cell membrane characteristics. DMSO-induced potentiation of TNF cytostasis/cytotoxicity was restricted to myeloid leukemia cell lines. In non-myeloid cells such as fibrosarcomas, Myosarcomas, thymomas, or carcinomas, DMSO was found either not to alter or to inhibit TNF-induced cell death. The latter results are in good agreement with data reported by others who suggested that DMSO could act as a scavenger of TNF-induced toxic radical formation. The potential correlation in myeloid cells between DMSO-induced changes in the cells' differentiation status and DMSO-enhanced TNF-susceptibility is discussed.
Shivananda Nadiminti - One of the best experts on this subject based on the ideXlab platform.
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mitral valve myxosarcoma
Indian Journal of Thoracic and Cardiovascular Surgery, 2005Co-Authors: Vivek Jawali, Murali R Chakravarthy, Srinivasan Kolar, Murali Manohar, Tammanagowda Ainangowda Patil, J K Das, Gilbert Joseph, Jayaprakash Krishnamoorthy, Shivananda NadimintiAbstract:Myxosarcomas are rare and incidence fewer than 3-5% has been quoted1,2 and these tumors arising from the posterior mitral leaflet (PML) is yet to be reported. The myxosarcoma was seen arising from the PML prolapsing in the aorta, causing a gradient of 70 mmHg across the aortic valve. In order to excise the tumor completely, the mitral valve had to be excised and replaced. Despite an echocardiographic confirmation of complete excision of the tumor, the tumor recurred. The histopathological examination showed both benign and malignant feature in the tumor.
