The Experts below are selected from a list of 231 Experts worldwide ranked by ideXlab platform
Zi-jian Song - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers
Analytical biochemistry, 2020Co-Authors: Yi Gao, Qian Liu, Sheng-sheng Liu, Zi-jian SongAbstract:Abstract Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA–NHS–Ester is brought about via activating three –OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P
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establishment of a 1 4 7 10 tetraazacyclododecane 1 4 7 10 tetraacetic acid mono n hydroxysuccinimide Ester dota nhs Ester based lectin microarray for efficiently detecting serum glycans in gastric cancers
Analytical Biochemistry, 2020Co-Authors: Songguo Li, Zi-jian Song, Lei Ye, Weidong DuAbstract:Abstract Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA–NHS–Ester is brought about via activating three –OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P
Weidong Du - One of the best experts on this subject based on the ideXlab platform.
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establishment of a 1 4 7 10 tetraazacyclododecane 1 4 7 10 tetraacetic acid mono n hydroxysuccinimide Ester dota nhs Ester based lectin microarray for efficiently detecting serum glycans in gastric cancers
Analytical Biochemistry, 2020Co-Authors: Songguo Li, Zi-jian Song, Lei Ye, Weidong DuAbstract:Abstract Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA–NHS–Ester is brought about via activating three –OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P
Hua-shan Zhang - One of the best experts on this subject based on the ideXlab platform.
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determination of biogenic amines in apples and wine with 8 phenyl 4 oxy acetic acid n hydroxysuccinimide Ester 4 4 difluoro 1 3 5 7 tetramethyl 4 bora 3a 4a diaza s indacene by high performance liquid chromatography
Analytica Chimica Acta, 2006Co-Authors: Jinshu Li, Kejing Huang, Hong Wang, Hua-shan ZhangAbstract:A precolumn-derivatization high performance liquid chromatographic method for biogenic amine analysis has been developed. Derivatization of biogenic amines was performed with a new fluorescent reagent, 8-phenyl-(4-oxy-acetic acid N-Hydroxysuccinimide Ester)-4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene (TMPAB-OSu), which was synthesized in our lab. The derivatization conditions and the influence of elution composition on the separation were investigated. In pH 8.5 H3BO3–Na2B4O7 media, the reaction of biogenic amines with TMPAB-OSu completed at 45 °C for 5 min. The labeled biogenic amines were separated on an ZORBAX Eclipse XDB-C8 column (150 mm × 4.6 mm, 5 μm) and detected with fluorescence at excitation and emission wavelengths of 497 and 509 nm, respectively. Detection limits of biogenic amines were 0.1–4 nmol L−1, at a signal-to-noise ratio of 3. The proposed method has been applied to the quantitative determination of spermine, phenethylamine, spermidine, cadaverine and putrescine in pericarp and pulp of mature and immature apples and wine with recoveries of 95–102% and R.S.D. of 0.7–3.9.
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2,6‐Dimethyl‐4‐quinolinecarboxylic acid N‐hydroxysuccinimide Ester: A fluorogenic hydrophilic derivatizing reagent for liquid chromatographic analysis of aliphatic amines
Journal of separation science, 2006Co-Authors: Hong Wang, Xin Liu, Niu Zhang, Li-wei Cao, Hua-shan ZhangAbstract:The use of a fluorogenic, hydrophilic, and amine-reactive reagent, 2,6-dimethyl-4-quinolinecarboxylic acid N-Hydroxysuccinimide Ester (DMQC-OSu) has been investigated in the procolumn derivatization for the LC separation of aliphatic amines. In pH 8.0 aqueous medium, DMQC-OSu reacted with amines at 50 degrees C within 20 min to form highly fluorescent carboxamides and the excess reagent hydrolyzed to the corresponding carboxylic acid. The separation of representative amine derivatives with DMQC-OSu has been performed using a C18 column with the fluorescence detection at 326/409 nm. The detection limits reached nanomolar level. The proposed method has been applied to the analysis of real samples with recoveries of 94-108%. Compared with other succinimidyl Esters used in the derivatization of amino compounds, DMQC-OSu and its hydrolysate had negligible fluorescence (phi(fl) = 0.09 and 0.02, respectively), which implied that small peaks appeared in chromatograms and slight interference was introduced to the determination.
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Separation of Amines as their 6-Methyl-2-phenyl-4-quinolinecarboxylic Acid N-Hydroxysuccinimide Ester Derivatives by High Performance Liquid Chromatography
Chromatographia, 2004Co-Authors: Hong Wang, Xin Liu, Hua-shan ZhangAbstract:6-Methyl-2-phenyl-4-quinolinecarboxylic acid N-Hydroxysuccinimide Ester (MPQC-OSu) has been developed for precolumn derivatization of aliphatic amines in HPLC. The great difference between the fluorescence quantum yield of MPQC-OSu derivative and that of MPQC-OSu hydrolysate, 6-methyl-2-phenyl-4-quinolinecarboxylic acid (MPQC) makes this reagent suitable for amine-labeling, followed by chromatographic separation. In pH8.5 borate buffer, MPQC-OSu reacted with amines at 60°C for 12 min to form highly fluorescent carboxamides and excess reagent hydrolysed to MPQC. The chromatographic behavior of amine derivatives with MPQC-OSu has been investigated using HPLC on C18 and C8 columns, respectively, with fluorescence detection at 340–402nm. A baseline separation of isopropanolamine, methylamine, ethylamine, n-propylamine, n-butylamine, n-amylamine and n-hexylamine was obtained in 25min using isocratic elution on a C18 column using methanol-water (70:30, v/v) as eluent. Detection limits were in the range 0.13–1 nM.
Songguo Li - One of the best experts on this subject based on the ideXlab platform.
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establishment of a 1 4 7 10 tetraazacyclododecane 1 4 7 10 tetraacetic acid mono n hydroxysuccinimide Ester dota nhs Ester based lectin microarray for efficiently detecting serum glycans in gastric cancers
Analytical Biochemistry, 2020Co-Authors: Songguo Li, Zi-jian Song, Lei Ye, Weidong DuAbstract:Abstract Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA–NHS–Ester is brought about via activating three –OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P
Yi Gao - One of the best experts on this subject based on the ideXlab platform.
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Establishment of a 1, 4, 7, 10-tetraazacyclododecane-1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) based lectin microarray for efficiently detecting serum glycans in gastric cancers
Analytical biochemistry, 2020Co-Authors: Yi Gao, Qian Liu, Sheng-sheng Liu, Zi-jian SongAbstract:Abstract Development of cancers is involved in changes of a variety of glycans. Lectin microarray is one of the most powerful methodologies for investigation of glycan alterations in biological samples with its advantages of high through-put, selectivity and specificity of the technique. However, utilization of lectin microarrays available commercially keeps of great challenges. In this study, we took use of the molecular self-assembled monolayer technique to modify a gold surface with the reagent 1,4,7,10-tetraazacyclododecane- 1,4,7,10-tetraacetic acid mono-N-Hydroxysuccinimide Ester (DOTA–NHS–Ester) in combination with 16-amino-1-hexadecanethiol hydrochloride. Cross-linking effect of DOTA–NHS–Ester is brought about via activating three –OH ends to three terminals of succinylimidines, making selective binding of the terminal amino groups in proteins possible. We immobilized ten commercial lectins on the platform and measured changes of serum lectin-matched glycans in patients with gastric cancer. The results demonstrated that this biochip modification platform conferred impressive chemical surface stabilization, sensitivity and geometric images. We observed that all the serum glycans tested in the patients were significantly higher than those in the controls (P
