The Experts below are selected from a list of 9783 Experts worldwide ranked by ideXlab platform
Ryoji Takahashi - One of the best experts on this subject based on the ideXlab platform.
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Loss-of-Function Mutation of Soybean R2R3 MYB Transcription Factor Dilutes Tawny Pubescence Color.
Frontiers in plant science, 2020Co-Authors: Fan Yan, Stephen M. Githiri, Yu Sang, Qingyu Wang, Yajing Liu, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulate their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color.
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loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny pubescence color
Frontiers in Plant Science, 2020Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.
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Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny pubescence color
2019Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute pubescence color.
Stephen M. Githiri - One of the best experts on this subject based on the ideXlab platform.
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Loss-of-Function Mutation of Soybean R2R3 MYB Transcription Factor Dilutes Tawny Pubescence Color.
Frontiers in plant science, 2020Co-Authors: Fan Yan, Stephen M. Githiri, Yu Sang, Qingyu Wang, Yajing Liu, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulate their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color.
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loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny pubescence color
Frontiers in Plant Science, 2020Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.
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Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny pubescence color
2019Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute pubescence color.
Qingyu Wang - One of the best experts on this subject based on the ideXlab platform.
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Loss-of-Function Mutation of Soybean R2R3 MYB Transcription Factor Dilutes Tawny Pubescence Color.
Frontiers in plant science, 2020Co-Authors: Fan Yan, Stephen M. Githiri, Yu Sang, Qingyu Wang, Yajing Liu, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulate their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color.
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loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny pubescence color
Frontiers in Plant Science, 2020Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.
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Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny pubescence color
2019Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute pubescence color.
Yu Sang - One of the best experts on this subject based on the ideXlab platform.
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Loss-of-Function Mutation of Soybean R2R3 MYB Transcription Factor Dilutes Tawny Pubescence Color.
Frontiers in plant science, 2020Co-Authors: Fan Yan, Stephen M. Githiri, Yu Sang, Qingyu Wang, Yajing Liu, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulate their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color.
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loss of function mutation of soybean r2r3 myb transcription factor dilutes tawny pubescence color
Frontiers in Plant Science, 2020Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. These results strongly suggest that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene.
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Loss-of-function mutation of soybean R2R3 MYB transcription factor reduces flavone content and dilutes tawny pubescence color
2019Co-Authors: Stephen M. Githiri, Yu Sang, Qingyu Wang, Ryoji TakahashiAbstract:Abstract Background Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are catalyzed by two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Results Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild-type of Glyma.03G258700 protein binds to the promoter of FNS II genes and upregulates their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein fails to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color. Conclusions This study revealed that soybean Glyma.03G258700 encoding the R2R3 MYB transcription factor corresponds to the Td gene. The wild type of MYB protein binds to the promoter of FNS II genes and upregulates their expression, resulting in higher flavone content and deeper pubescence color. Loss-of-function mutation of the gene fails to promote expression of FNS II genes, resulting in lower flavone content and dilute pubescence color.
Fan Yan - One of the best experts on this subject based on the ideXlab platform.
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Loss-of-Function Mutation of Soybean R2R3 MYB Transcription Factor Dilutes Tawny Pubescence Color.
Frontiers in plant science, 2020Co-Authors: Fan Yan, Stephen M. Githiri, Yu Sang, Qingyu Wang, Yajing Liu, Ryoji TakahashiAbstract:Pubescence color of soybean is controlled by two genes, T and Td. In the presence of a dominant T allele, dominant and recessive alleles of the Td locus generate tawny and light tawny (or near-gray) pubescence, respectively. Flavones, responsible for pubescence color, are synthesized via two copies of flavone synthase II genes (FNS II-1 and FNS II-2). This study was conducted to map and clone the Td gene. Genetic and linkage analysis using an F2 population and F3 families derived from a cross between a Clark near-Isogenic Line with light tawny pubescence (genotype: TT tdtd) and a Harosoy near-Isogenic Line with tawny pubescence (TT TdTd) revealed a single gene for pubescence color around the end of chromosome 3. Genome sequence alignment of plant introductions revealed an association between premature stop codons in Glyma.03G258700 (R2R3 MYB transcription factor) and recessive td allele. Cultivars and Lines having near-gray or light tawny pubescence and a gray pubescence cultivar with td allele had premature stop codons in the gene. These results suggest that Glyma.03G258700 corresponds to the Td gene. It was predominantly expressed in pubescence. Compared to a tawny pubescence Line, a near-Isogenic Line with td allele produced extremely small amounts of transcripts of Glyma.03G258700, FNS II-1, and FNS II-2 in pubescence. The promoter of FNS II-1 and FNS II-2 shared cis-acting regulatory elements for binding of MYB proteins. These results suggest that the wild type of Glyma.03G258700 protein may bind to the promoter of FNS II genes and upregulate their expression, resulting in increased flavone content and deeper pubescence color. In contrast, mutated Glyma.03G258700 protein may fail to upregulate the expression of FNS II genes, resulting in decreased flavone content and dilute pubescence color.
