The Experts below are selected from a list of 72 Experts worldwide ranked by ideXlab platform
Solange Oliveira - One of the best experts on this subject based on the ideXlab platform.
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the complete genome sequence of a new Necrovirus isolated from olea europaea l
Archives of Virology, 2005Co-Authors: Joana M S Cardoso, Maria Do Rosario Felix, Maria Ivone E Clara, Solange OliveiraAbstract:The complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals highest sequence identity with that of TNV-D. Based on the deduced genome organization and phylogenetic analysis of predicted functional translation products with that of other Necroviruses, the GP isolate genome appears to represent an example of a new virus arisen by gene exchange and is proposed to be a new Necrovirus, provisionally named Olive mild mosaic virus.
Yuzi Liu - One of the best experts on this subject based on the ideXlab platform.
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the complete nucleotide sequence of beet black scorch virus bbsv a new member of the genus Necrovirus
Archives of Virology, 2002Co-Authors: Y Cao, Zhunan Cai, Q Ding, Chenggui Han, Yuzi LiuAbstract:The complete nucleotide sequence of Beet black scorch virus (BBSV) was determined. The BBSV genome is composed of 3641 nucleotides and has similar organization with Tobacco necrosis virus D of 61% nucleotide identity. The 5'-proximal open reading frame (ORF) encodes a putative 23 kDa protein and a 82 kDa protein by reading-through of an amber termination codon. Three small ORFs located in the center of the genome may encode for a 4.2 kDa protein and two 7 kDa proteins. The 3'-proximal ORF encodes a 24.5 kDa protein equivalent in mass to the viral coat protein. Considering biological and molecular similarities with TNV, it is concluded that BBSV is a new member of the genus Necrovirus.
Maria Ivone E Clara - One of the best experts on this subject based on the ideXlab platform.
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the complete genome sequence of a new Necrovirus isolated from olea europaea l
Archives of Virology, 2005Co-Authors: Joana M S Cardoso, Maria Do Rosario Felix, Maria Ivone E Clara, Solange OliveiraAbstract:The complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals highest sequence identity with that of TNV-D. Based on the deduced genome organization and phylogenetic analysis of predicted functional translation products with that of other Necroviruses, the GP isolate genome appears to represent an example of a new virus arisen by gene exchange and is proposed to be a new Necrovirus, provisionally named Olive mild mosaic virus.
Maria Do Rosario Felix - One of the best experts on this subject based on the ideXlab platform.
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the complete genome sequence of a new Necrovirus isolated from olea europaea l
Archives of Virology, 2005Co-Authors: Joana M S Cardoso, Maria Do Rosario Felix, Maria Ivone E Clara, Solange OliveiraAbstract:The complete nucleotide sequence of a virus isolated from Olea europaea L. (GP isolate), previously identified as an isolate of Tobacco necrosis virus D (TNV-D) based on its coat protein sequence, was determined. The viral RNA genome consists of 3683 nucleotides and contains five open reading frames. The putative RNA-dependent RNA polymerase shows 91.2% amino acid identity with that of an isolate of Olive latent virus 1 (OLV-1) and the coat protein reveals highest sequence identity with that of TNV-D. Based on the deduced genome organization and phylogenetic analysis of predicted functional translation products with that of other Necroviruses, the GP isolate genome appears to represent an example of a new virus arisen by gene exchange and is proposed to be a new Necrovirus, provisionally named Olive mild mosaic virus.
Melvin D. Bolton - One of the best experts on this subject based on the ideXlab platform.
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RNAseq Analysis of Rhizomania-Infected Sugar Beet Provides the First Genome Sequence of Beet Necrotic Yellow Vein Virus from the USA and Identifies a Novel AlphaNecrovirus and Putative Satellite Viruses.
Viruses, 2020Co-Authors: John J. Weiland, Roshan Sharma Poudel, Alyssa Flobinus, David Cook, Gary A. Secor, Melvin D. BoltonAbstract:“Rhizomania” of sugar beet is a soilborne disease complex comprised of beet necrotic yellow vein virus (BNYVV) and its plasmodiophorid vector, Polymyxa betae. Although BNYVV is considered the causal agent of rhizomania, additional viruses frequently accompany BNYVV in diseased roots. In an effort to better understand the virus cohort present in sugar beet roots exhibiting rhizomania disease symptoms, five independent RNA samples prepared from diseased beet seedlings reared in a greenhouse or from field-grown adult sugar beet plants and enriched for virus particles were subjected to RNAseq. In all but a healthy control sample, the technique was successful at identifying BNYVV and provided sequence reads of sufficient quantity and overlap to assemble > 98% of the published genome of the virus. Utilizing the derived consensus sequence of BNYVV, infectious RNA was produced from cDNA clones of RNAs 1 and 2. The approach also enabled the detection of beet soilborne mosaic virus (BSBMV), beet soilborne virus (BSBV), beet black scorch virus (BBSV), and beet virus Q (BVQ), with near-complete genome assembly afforded to BSBMV and BBSV. In one field sample, a novel virus sequence of 3682 nt was assembled with significant sequence similarity and open reading frame (ORF) organization to members within the subgenus AlphaNecrovirus (genus Necrovirus; family Tombusviridae). Construction of a DNA clone based on this sequence led to the production of the novel RNA genome in vitro that was capable of inducing local lesion formation on leaves of Chenopodium quinoa. Additionally, two previously unreported satellite viruses were revealed in the study; one possessing weak similarity to satellite maize white line mosaic virus and a second possessing moderate similarity to satellite tobacco necrosis virus C. Taken together, the approach provides an efficient pipeline to characterize variation in the BNYVV genome and to document the presence of other viruses potentially associated with disease severity or the ability to overcome resistance genes used for sugar beet rhizomania disease management.
