The Experts below are selected from a list of 18909 Experts worldwide ranked by ideXlab platform
Yueping Yin - One of the best experts on this subject based on the ideXlab platform.
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gonococcal urethritis caused by a multidrug resistant Neisseria gonorrhoeae strain with high level resistance to spectinomycin in china
Emerging microbes & infections, 2020Co-Authors: Shaochun Chen, Xiaoyu Zhu, Yueping YinAbstract:We report a recent (2018) gonorrhoeal urethritis caused by a multidrug-resistant Neisseria gonorrhoeae strain in China. The isolated N. gonorrhoeae strain from a male and female pair expressed high...
Monica M Lahra - One of the best experts on this subject based on the ideXlab platform.
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direct detection of pena gene associated with ceftriaxone resistant Neisseria gonorrhoeae fc428 strain by using pcr
Emerging Infectious Diseases, 2018Co-Authors: David M Whiley, Lebogang P Mhango, Amy V Jennison, Graeme R Nimmo, Monica M LahraAbstract:The ceftriaxone-resistant Neisseria gonorrhoeae FC428 clone was first observed in Japan in 2015, and in 2017, it was documented in Denmark, Canada, and Australia. Here, we describe a PCR for direct detection of the penA gene associated with this strain that can be used to enhance surveillance activities.
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multitarget pcr assay for direct detection of penicillinase producing Neisseria gonorrhoeae for enhanced surveillance of gonococcal antimicrobial resistance
Journal of Clinical Microbiology, 2015Co-Authors: Cameron Buckley, Ella Trembizki, Namraj Goire, Kevin Freeman, Robert W Baird, Marcus Y Chen, Basil Donovan, Rebecca Guy, Monica M LahraAbstract:A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.
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penicillinase producing plasmid types in Neisseria gonorrhoeae clinical isolates from australia
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: David M Whiley, Ella Trembizki, Cameron Buckley, Andrew J Lawrence, Kevin Freeman, Athena Limnios, Julie C Pearson, Helen V Smith, Kerrie Stevens, Monica M LahraAbstract:Penicillinase-producing Neisseria gonorrhoeae (PPNG) carrying the blaTEM-135 gene is of particular concern, as it is considered a stepping stone toward resistance to extended-spectrum cephalosporins. Here, we sought to characterize plasmid types and the occurrence of the blaTEM-135 gene for N. gonorrhoeae clinical isolates from Australia. We found that blaTEM-135 was prevalent in Australian PPNG and was detected on all three major plasmid types.
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characterization of a novel Neisseria gonorrhoeae penicillinase producing plasmid isolated in australia in 2012
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: Ella Trembizki, Cameron Buckley, Andrew J Lawrence, Monica M Lahra, David M WhileyAbstract:Neisseria gonorrhoeae plasmids harboring a penicillinase gene are responsible for dissemination of high-level penicillin resistance among gonococci worldwide. Penicillinase-producing N. gonorrhoeae (PPNG) strains are believed to have originally acquired such plasmids via horizontal genetic transfer
Yonatan H Grad - One of the best experts on this subject based on the ideXlab platform.
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increased power from conditional bacterial genome wide association identifies macrolide resistance mutations in Neisseria gonorrhoeae
Nature Communications, 2020Co-Authors: Kevin C, Yonatan H Grad, Tatum D Mortimer, Marissa A Duckett, Allison L Hicks, Nicole E Wheeler, Leonor SanchezbusoAbstract:The emergence of resistance to azithromycin complicates treatment of Neisseria gonorrhoeae, the etiologic agent of gonorrhea. Substantial azithromycin resistance remains unexplained after accounting for known resistance mutations. Bacterial genome-wide association studies (GWAS) can identify novel resistance genes but must control for genetic confounders while maintaining power. Here, we show that compared to single-locus GWAS, conducting GWAS conditioned on known resistance mutations reduces the number of false positives and identifies a G70D mutation in the RplD 50S ribosomal protein L4 as significantly associated with increased azithromycin resistance (p-value = 1.08 × 10−11). We experimentally confirm our GWAS results and demonstrate that RplD G70D and other macrolide binding site mutations are prevalent (present in 5.42% of 4850 isolates) and widespread (identified in 21/65 countries across two decades). Overall, our findings demonstrate the utility of conditional associations for improving the performance of microbial GWAS and advance our understanding of the genetic basis of macrolide resistance. The mechanisms underlying resistance of Neisseria gonorrhoeae to the antibiotic azithromycin are incompletely understood. Here, Ma et al. conduct a conditional genome-wide association study to identify new resistance mutations and experimentally confirm that a mutation in ribosomal protein L4 confers increased resistance.
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azithromycin susceptibility among Neisseria gonorrhoeae isolates and seasonal macrolide use
The Journal of Infectious Diseases, 2019Co-Authors: Scott W Olesen, Elizabeth Torrone, John R Papp, Robert D Kirkcaldy, Marc Lipsitch, Yonatan H GradAbstract:Rising azithromycin nonsusceptibility among Neisseria gonorrhoeae isolates threatens current treatment recommendations, but the cause of this rise is not well understood. We performed an ecological study of seasonal patterns in macrolide use and azithromycin resistance in N. gonorrhoeae, finding that population-wide macrolide use is associated with increased azithromycin nonsusceptibility. These results, indicative of bystander selection, have implications for antibiotic prescribing guidelines.
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in vitro selection of Neisseria gonorrhoeae mutants with elevated mic values and increased resistance to cephalosporins
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: Stevenr Johnson, Marc Lipsitch, Yonatan H Grad, Satishkumar Ranganathan Ganakammal, Mark Burroughs, Mike Frace, Ryan Weil, David L TreesAbstract:Strains of Neisseria gonorrhoeae with mosaic penA genes bearing novel point mutations in penA have been isolated from ceftriaxone treatment failures. Such isolates exhibit significantly higher MIC values to third-generation cephalosporins. Here we report the in vitro isolation of two mutants with elevated MICs to cephalosporins. The first possesses a point mutation in the transpeptidase region of the mosaic penA gene, and the second contains an insertion mutation in pilQ.
Shaochun Chen - One of the best experts on this subject based on the ideXlab platform.
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gonococcal urethritis caused by a multidrug resistant Neisseria gonorrhoeae strain with high level resistance to spectinomycin in china
Emerging microbes & infections, 2020Co-Authors: Shaochun Chen, Xiaoyu Zhu, Yueping YinAbstract:We report a recent (2018) gonorrhoeal urethritis caused by a multidrug-resistant Neisseria gonorrhoeae strain in China. The isolated N. gonorrhoeae strain from a male and female pair expressed high...
Magnus Unemo - One of the best experts on this subject based on the ideXlab platform.
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Genomic Epidemiology of Azithromycin-Nonsusceptible Neisseria gonorrhoeae, Argentina, 2005–2019
'Centers for Disease Control and Prevention (CDC)', 2021Co-Authors: Ricardo Ariel Gianecini, Magnus Unemo, Daniel Golparian, Tomas Poklepovich, Noelia Cuenca, Ezequiel Tuduri, Josefina Campos, Patricia GalarzaAbstract:Azithromycin-nonsusceptible Neisseria gonorrhoeae strains are an emerging global public health threat. During 2015–2018, the prevalence of azithromycin-nonsusceptible gonococcal infection increased significantly in Argentina. To investigate the genomic epidemiology and resistance mechanisms of these strains, we sequenced 96 nonsusceptible isolates collected in Argentina during 2005–2019. Phylogenomic analysis revealed 2 main clades, which were characterized by a limited geographic distribution, circulating during January 2015–November 2019. These clades included the internationally spreading multilocus sequence types (STs) 1580 and 9363. The ST1580 isolates, which had MICs of 2–4 μg/mL, had mutations in the 23S rRNA. The ST9363 isolates, which had MICs of 2–4 or >256 μg/mL, had mutations in the 23S rRNA, a mosaic mtr locus, or both. Identifying the geographic dissemination and characteristics of these predominant clones will guide public health policies to control the spread of azithromycin-nonsusceptible N. gonorrhoeae in Argentina
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Multidrug-resistant Neisseria gonorrhoeae infection with ceftriaxone resistance and intermediate resistance to azithromycin, Denmark, 2017.
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, 2017Co-Authors: David Terkelsen, Janne Schurmann Tolstrup, Camilla Hundahl Johnsen, Ole Lund, Helle Kiellberg Larsen, Peder Worning, Magnus Unemo, Henrik WesthAbstract:We describe a multidrug-resistant Neisseria gonorrhoeae infection with ceftriaxone resistance and azithromycin intermediate resistance in a heterosexual man in Denmark, 2017. Whole genome sequencing of the strain GK124 identified MSLT ST1903, NG-MAST ST1614 and all relevant resistance determinants including similar penA resistance mutations previously described in ceftriaxone-resistant gonococcal strains. Although treatment with ceftriaxone 0.5 g plus azithromycin 2 g was successful, increased awareness of spread of gonococcal strains threatening the recommended dual therapy is crucial.
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analytical specificity and sensitivity of the novel dual target geneproof Neisseria gonorrhoeae pcr kit for detection of n gonorrhoeae
Apmis, 2015Co-Authors: Daniel Golparian, Bengt Hellmark, Magnus UnemoAbstract:Detection of Neisseria gonorrhoeae relies increasingly on nucleic acid amplification tests (NAATs). The specificity of many gonococcal NAATs has been suboptimal and supplementary testing remains recommended in Europe and several additional countries. The novel dual-target GeneProof Neisseria gonorrhoeae PCR kit, targeting porA pseudogene and 16S rRNA gene, showed a high specificity and sensitivity when isolates of non-gonococcal Neisseria and related species (n = 144), and gonococci (n = 104) were tested. However, rare gonococcal porA mutants were only detected in the 16S rRNA gene target and two non-gonococcal isolates showed a low-level cross-reactivity in the 16S rRNA gene target. The detection limit for both targets was 1.5 copies per reaction.
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first three Neisseria gonorrhoeae isolates with high level resistance to azithromycin in sweden a threat to currently available dual antimicrobial regimens for treatment of gonorrhea
Antimicrobial Agents and Chemotherapy, 2014Co-Authors: Magnus Unemo, Daniel Golparian, Bengt HellmarkAbstract:Neisseria gonorrhoeae has developed resistance to all antimicrobials previously used for first-line treatment of gonorrhea (1).…
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first Neisseria gonorrhoeae strain with resistance to cefixime causing gonorrhoea treatment failure in austria 2011
Eurosurveillance, 2011Co-Authors: Magnus Unemo, Daniel Golparian, A Stary, A EigentlerAbstract:We describe the first cefixime-resistant Neisseria gonorrhoeae strain in Austria that caused treatment failure. It follows the first five cases in Europe of cefixime treatment failure, reported in Norway in 2010 and the United Kingdom in 2011. Effective treatment of gonorrhoea is crucial for public health control and, at present, requires substantially enhanced awareness, more frequent test-of-cure, interaction with experts after therapeutic failure, tracing and therapy of contacts, and surveillance of gonococcal antimicrobial resistance and treatment failures worldwide.
