The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
J. Carlos Pastor - One of the best experts on this subject based on the ideXlab platform.
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of ophthalmic inflammation and infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raúl Ortiz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola.
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of Ophthalmic Inflammation and Infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raul Ortíz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola. Findings Neonatal conjunctival smears ( n = 95), maternal data, and perinatal factors were collected. Slides were air-dried and sent to the Microbiology Department of the Hospital Clinico Universitario, Valladolid, Spain, where trained personnel performed Gram and methylene blue staining methods. Findings were interpreted by two expert microbiologists. Ophthalmological examination of all children showed five newborns with clinical signs of ON. Fourteen mothers reported were suspected with vulvo-vaginitis, and 27 had a urinary infection during pregnancy. Gram staining revealed the presence of epithelial cells in 87.6% and leukocytes in 15% of the conjunctival smears. These values were significantly higher than those shown by methylene blue staining. No rods, cocci, or yeasts were identified by either staining method. Chlamydia trachomatis DNA was also undetected in a small sub-sample with clinical suspicion of ON. There was no correlation among the presence of ON, ON microbes, maternal data, or perinatal factors. Conclusions Basic microbiological techniques did not provide enough information for screening cases of ON in Angola. Therefore, the use of molecular biology or other techniques is warranted for this purpose.
Anna Roca - One of the best experts on this subject based on the ideXlab platform.
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Does azithromycin given to women in labour decrease ocular bacterial infection in neonates? A double-blind, randomized trial.
BMC infectious diseases, 2017Co-Authors: Sarah E. Burr, Bully Camara, Claire Oluwalana, Ebrima Bojang, Christian Bottomley, Abdoulie Bojang, Robin L. Bailey, Umberto D'alessandro, Anna RocaAbstract:Vertical transmission can result in Neonatal infection and disease. Reducing the transmission of bacterial pathogens from mother to infant may be an effective means of preventing Neonatal infection, including bacterial Conjunctivitis. In a double-blind, randomized trial, we assessed the effect of administering a single dose of oral azithromycin to women in labour on bacterial colonization of the neonate. A reduction in purulent Neonatal Conjunctivitis was a secondary objective of the trial. Ocular samples were collected from the lower fornix of infants presenting with clinical signs of purulent Conjunctivitis during the first eight weeks of life. Incidence of purulent Conjunctivitis was compared between trial arms. Bacterial infection was assessed using PCR and incidence of purulent Conjunctivitis due to bacteria was also compared between arms. Forty of 843 infants (4.7%) presented clinical signs of purulent Conjunctivitis. No significant difference in incidence of purulent Conjunctivitis was seen between azithromycin and placebo arms [4.3% (18/419) versus 5.2% (22/424), OR = 0.82, 95% CI (0.44,1.54), p = 0.628]. S. aureus was the most commonly identified pathogen, detected in 38% of cases. Incidence of purulent-Conjunctivitis due to bacterial infection was lower in the azithromycin arm [1.2% (5/419) versus 3.8% (16/424), OR = 0.31, 95% CI (0.12–0.82), p = 0.025)]. The incidence of gram-positive bacteria was also lower in the azithromycin arm [1.0% (4/419) versus 3.3% (14/424), OR = 0.28, 95%CI (0.10–0.82), p = 0.029]. Oral azithromycin given to women during labour may have the potential to reduce the incidence of bacterial Neonatal Conjunctivitis. ClinicalTrials.gov, identifier NCT01800942 , registration date 26 Feb 2013.
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Does azithromycin given to women in labour decrease ocular bacterial infection in neonates? A double-blind, randomized trial
BMC Infectious Diseases, 2017Co-Authors: Sarah E. Burr, Bully Camara, Claire Oluwalana, Ebrima Bojang, Christian Bottomley, Abdoulie Bojang, Robin L. Bailey, Umberto D’alessandro, Anna RocaAbstract:Background Vertical transmission can result in Neonatal infection and disease. Reducing the transmission of bacterial pathogens from mother to infant may be an effective means of preventing Neonatal infection, including bacterial Conjunctivitis. Methods In a double-blind, randomized trial, we assessed the effect of administering a single dose of oral azithromycin to women in labour on bacterial colonization of the neonate. A reduction in purulent Neonatal Conjunctivitis was a secondary objective of the trial. Ocular samples were collected from the lower fornix of infants presenting with clinical signs of purulent Conjunctivitis during the first eight weeks of life. Incidence of purulent Conjunctivitis was compared between trial arms. Bacterial infection was assessed using PCR and incidence of purulent Conjunctivitis due to bacteria was also compared between arms. Results Forty of 843 infants (4.7%) presented clinical signs of purulent Conjunctivitis. No significant difference in incidence of purulent Conjunctivitis was seen between azithromycin and placebo arms [4.3% (18/419) versus 5.2% (22/424), OR = 0.82, 95% CI (0.44,1.54), p = 0.628]. S. aureus was the most commonly identified pathogen, detected in 38% of cases. Incidence of purulent-Conjunctivitis due to bacterial infection was lower in the azithromycin arm [1.2% (5/419) versus 3.8% (16/424), OR = 0.31, 95% CI (0.12–0.82), p = 0.025)]. The incidence of gram-positive bacteria was also lower in the azithromycin arm [1.0% (4/419) versus 3.3% (14/424), OR = 0.28, 95%CI (0.10–0.82), p = 0.029]. Conclusions Oral azithromycin given to women during labour may have the potential to reduce the incidence of bacterial Neonatal Conjunctivitis. Trial registration ClinicalTrials.gov, identifier NCT01800942 , registration date 26 Feb 2013.
Mark Wood - One of the best experts on this subject based on the ideXlab platform.
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a controlled trial of povidone iodine as prophylaxis against ophthalmia neonatorum
The New England Journal of Medicine, 1995Co-Authors: Sherwin J. Isenberg, Mark WoodAbstract:Background Neonatal Conjunctivitis (ophthalmia neonatorum) continues to cause blindness, because the agents used prophylactically to prevent this condition are not completely effective and are not widely available in many parts of the world. Povidone–iodine ophthalmic solution is an effective antibacterial agent with broad antibacterial and antiviral activity to which no bacteria are known to be resistant, and it is far less expensive and less toxic than the agents currently used to prevent Neonatal Conjunctivitis. Methods We conducted a masked, prospective trial involving 3117 infants born over a period of 30 months in a hospital in Kenya. Shortly after birth each infant received a 2.5 percent solution of povidone–iodine (n = 1076), a 1 percent solution of silver nitrate (n = 929), or 0.5 percent erythromycin ointment (n = 1112) in both eyes. Randomization was achieved by rotating the three medications after each was used for a week. Results Of the neonates treated with povidone–iodine, 13.1 percent had ...
Isabel Alexandre - One of the best experts on this subject based on the ideXlab platform.
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Vertical transmission of bacterial eye infections, Angola, 2011-2012.
Emerging infectious diseases, 2015Co-Authors: Mar Justel, Isabel Alexandre, Itziar Fernández, Prudencio Martínez, Ivan Sanz, Ana Rodriguez-fernandez, José Carlos Pastor, Raúl Ortiz De LejarazuAbstract:To determine transmission rates for Neonatal Conjunctivitis causative microorganisms in Angola, we analyzed 312 endocervical and 255 conjunctival samples from mothers and newborns, respectively, during 2011–2012. Transmission rates were 50% for Chlamydia trachomatis and Neisseria gonorrhoeae and 10.5% for Mycoplasma genitalium. Possible pathogenic effects of M. genitalium in children’s eyes are unknown.
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Vertical Transmission of Bacterial Eye Infections, Angola, 2011–2012
Centers for Disease Control and Prevention, 2015Co-Authors: Mar Justel, Isabel Alexandre, Itziar Fernández, Prudencio Martínez, Ivan Sanz, Ana Rodriguez-fernandez, José Carlos Pastor, Raúl Ortiz De LejarazuAbstract:To determine transmission rates for Neonatal Conjunctivitis causative microorganisms in Angola, we analyzed 312 endocervical and 255 conjunctival samples from mothers and newborns, respectively, during 2011–2012. Transmission rates were 50% for Chlamydia trachomatis and Neisseria gonorrhoeae and 10.5% for Mycoplasma genitalium. Possible pathogenic effects of M. genitalium in children’s eyes are unknown
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of ophthalmic inflammation and infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raúl Ortiz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola.
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of Ophthalmic Inflammation and Infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raul Ortíz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola. Findings Neonatal conjunctival smears ( n = 95), maternal data, and perinatal factors were collected. Slides were air-dried and sent to the Microbiology Department of the Hospital Clinico Universitario, Valladolid, Spain, where trained personnel performed Gram and methylene blue staining methods. Findings were interpreted by two expert microbiologists. Ophthalmological examination of all children showed five newborns with clinical signs of ON. Fourteen mothers reported were suspected with vulvo-vaginitis, and 27 had a urinary infection during pregnancy. Gram staining revealed the presence of epithelial cells in 87.6% and leukocytes in 15% of the conjunctival smears. These values were significantly higher than those shown by methylene blue staining. No rods, cocci, or yeasts were identified by either staining method. Chlamydia trachomatis DNA was also undetected in a small sub-sample with clinical suspicion of ON. There was no correlation among the presence of ON, ON microbes, maternal data, or perinatal factors. Conclusions Basic microbiological techniques did not provide enough information for screening cases of ON in Angola. Therefore, the use of molecular biology or other techniques is warranted for this purpose.
Itziar Fernández - One of the best experts on this subject based on the ideXlab platform.
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Vertical transmission of bacterial eye infections, Angola, 2011-2012.
Emerging infectious diseases, 2015Co-Authors: Mar Justel, Isabel Alexandre, Itziar Fernández, Prudencio Martínez, Ivan Sanz, Ana Rodriguez-fernandez, José Carlos Pastor, Raúl Ortiz De LejarazuAbstract:To determine transmission rates for Neonatal Conjunctivitis causative microorganisms in Angola, we analyzed 312 endocervical and 255 conjunctival samples from mothers and newborns, respectively, during 2011–2012. Transmission rates were 50% for Chlamydia trachomatis and Neisseria gonorrhoeae and 10.5% for Mycoplasma genitalium. Possible pathogenic effects of M. genitalium in children’s eyes are unknown.
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Vertical Transmission of Bacterial Eye Infections, Angola, 2011–2012
Centers for Disease Control and Prevention, 2015Co-Authors: Mar Justel, Isabel Alexandre, Itziar Fernández, Prudencio Martínez, Ivan Sanz, Ana Rodriguez-fernandez, José Carlos Pastor, Raúl Ortiz De LejarazuAbstract:To determine transmission rates for Neonatal Conjunctivitis causative microorganisms in Angola, we analyzed 312 endocervical and 255 conjunctival samples from mothers and newborns, respectively, during 2011–2012. Transmission rates were 50% for Chlamydia trachomatis and Neisseria gonorrhoeae and 10.5% for Mycoplasma genitalium. Possible pathogenic effects of M. genitalium in children’s eyes are unknown
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of ophthalmic inflammation and infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raúl Ortiz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola.
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The value of simple microbiological studies for on-site screening of acute Neonatal Conjunctivitis in Angola
Journal of Ophthalmic Inflammation and Infection, 2014Co-Authors: Isabel Alexandre, Nestor Cortes, Mar Justel, Itziar Fernández, Raul Ortíz De Lejarazu, J. Carlos PastorAbstract:Background Neonatal Conjunctivitis or ophthalmia neonatorum (ON) is an acute bacterial Conjunctivitis contracted by newborns during delivery. In non-industrialized countries, detection of the etiological agent is difficult due to the unavailability of modern diagnostic resources. Therefore, we analyzed the effectiveness of Gram and methylene blue staining techniques, which are simple microbiological methods in suspecting the aetiology of ON in a maternity ward in Luanda, Angola. Findings Neonatal conjunctival smears ( n = 95), maternal data, and perinatal factors were collected. Slides were air-dried and sent to the Microbiology Department of the Hospital Clinico Universitario, Valladolid, Spain, where trained personnel performed Gram and methylene blue staining methods. Findings were interpreted by two expert microbiologists. Ophthalmological examination of all children showed five newborns with clinical signs of ON. Fourteen mothers reported were suspected with vulvo-vaginitis, and 27 had a urinary infection during pregnancy. Gram staining revealed the presence of epithelial cells in 87.6% and leukocytes in 15% of the conjunctival smears. These values were significantly higher than those shown by methylene blue staining. No rods, cocci, or yeasts were identified by either staining method. Chlamydia trachomatis DNA was also undetected in a small sub-sample with clinical suspicion of ON. There was no correlation among the presence of ON, ON microbes, maternal data, or perinatal factors. Conclusions Basic microbiological techniques did not provide enough information for screening cases of ON in Angola. Therefore, the use of molecular biology or other techniques is warranted for this purpose.
