The Experts below are selected from a list of 3036 Experts worldwide ranked by ideXlab platform
Gregor Bachmannharildstad - One of the best experts on this subject based on the ideXlab platform.
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diagnostic values of beta 2 transferrin and beta trace protein as markers for cerebrospinal fluid fistula
Rhinology, 2008Co-Authors: Gregor BachmannharildstadAbstract:Introduction: During recent decades, β 2 -transferrin and β-trace protein (prostaglandin D synthase) have been used as immunological markers for the diagnosis of CSF fistula. A method for detecting CSF traces should be non invasive, reliable and cheap. Methods: The characteristics of the two immunological markers are described based on own experience and a literature review. PubMed (1966 - 2007) was searched and 39 articles were retrieved from the period 1987 - 2007. Results: The β 2 -transferrin marker showed a high reliability during the last decades using immunofixation or immunoblotting. The performance of β 2 -transferrin assay requires between two and four hours hands-on time in the laboratory depending on the assay. The β-trace protein protein marker showed a high reliability when assayed using immunoelectrophoresis or laser-Nephelometry. Laser-nephelomety is automated, non- time consuming, provides quantitative results and last but not least, is cheap. A cut-off point at 1.11 mg/l for β-trace protein gave the best trade-off between high sensitivity and high specificity when including the secretion/ serum ratio. Conclusion: Both β 2 -transferrin and β-trace protein are reliable immunological markers for the detection of CSF traces. High diagnostic accuracy values were found for both β 2 -transferrin and β-trace protein protein.
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diagnostic values of beta 2 transferrin and beta trace protein as markers for cerebrospinal fluid fistula
Rhinology, 2008Co-Authors: Gregor BachmannharildstadAbstract:Introduction During recent decades, beta2-transferrin and beta-trace protein (prostaglandin D synthase) have been used as immunological markers for the diagnosis of CSF fistula. A method for detecting CSF traces should be non invasive, reliable and cheap. Methods The characteristics of the two immunological markers are described based on own experience and a literature review. PubMed (1966-2007) was searched and 39 articles were retrieved from the period 1987-2007. Results The beta2-transferrin marker showed a high reliability during the last decades using immunofixation or immunoblotting. The performance of beta2-transferrin assay requires between two and four hours hands-on time in the laboratory depending on the assay. The beta-trace protein protein marker showed a high reliability when assayed using immunoelectrophoresis or laser-Nephelometry. Laser-nephelomety is automated, non- time consuming, provides quantitative results and last but not least, is cheap. A cut-off point at 1.11 mg/l for beta-trace protein gave the best trade-off between high sensitivity and high specificity when including the secretion/serum ratio. Conclusion Both beta2-transferrin and beta-trace protein are reliable immunological markers for the detection of CSF traces. High diagnostic accuracy values were found for both beta2-transferrin and beta-trace protein protein.
Cyle Wold - One of the best experts on this subject based on the ideXlab platform.
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strong spectral variation of biomass smoke light absorption and single scattering albedo observed with a novel dual wavelength photoacoustic instrument
Journal of Geophysical Research, 2008Co-Authors: K Lewis, W P Arnott, Hans Moosmuller, Cyle WoldAbstract:[1] A dual-wavelength photoacoustic instrument operating at 405 and 870 nm was used during the 2006 Fire Lab at Missoula Experiment to measure light scattering and absorption by smoke from the combustion of a variety of biomass fuels. Simultaneous measurements of aerosol light scattering by reciprocal Nephelometry within the instrument's acoustic resonator accompany photoacoustic aerosol light absorption measurements. Single scattering albedo values at 405 nm ranging from 0.37 to 0.95 were measured for different fuel types, and the spectral dependence of absorption was quantified using the Angstrom exponent of absorption. An absorption Angstrom exponent near unity is commonly observed for motor vehicle emission-generated black carbon aerosol. For biomass smoke, Angstrom exponents as high as 3.5 were found in association with smoke having single scattering albedo near unity. The measurements strongly suggest that light-absorbing organic material is present in wood smoke. A second single-wavelength photoacoustic instrument with reciprocal Nephelometry was used to quantify aerosol scattering and absorption at 532 nm. Absorption Angstrom exponents calculated using 532 and 870 nm data were as large as 2.5 for smoke with single scattering albedos near unity. The spectral variation in optical properties provides insight into the differentiation of aerosols from mobile or industrial sources versus those from biomass burning. Optical properties of biomass smokes could be classified by general fuel type such as flowering shrubs versus pine needle litter.
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strong spectral variation of biomass smoke light absorption and single scattering albedo observed with a novel dual wavelength photoacoustic instrument
Journal of Geophysical Research, 2008Co-Authors: K Lewis, W P Arnott, Hans Moosmuller, Cyle WoldAbstract:[1] A dual-wavelength photoacoustic instrument operating at 405 and 870 nm was used during the 2006 Fire Lab at Missoula Experiment to measure light scattering and absorption by smoke from the combustion of a variety of biomass fuels. Simultaneous measurements of aerosol light scattering by reciprocal Nephelometry within the instrument's acoustic resonator accompany photoacoustic aerosol light absorption measurements. Single scattering albedo values at 405 nm ranging from 0.37 to 0.95 were measured for different fuel types, and the spectral dependence of absorption was quantified using the Angstrom exponent of absorption. An absorption Angstrom exponent near unity is commonly observed for motor vehicle emission-generated black carbon aerosol. For biomass smoke, Angstrom exponents as high as 3.5 were found in association with smoke having single scattering albedo near unity. The measurements strongly suggest that light-absorbing organic material is present in wood smoke. A second single-wavelength photoacoustic instrument with reciprocal Nephelometry was used to quantify aerosol scattering and absorption at 532 nm. Absorption Angstrom exponents calculated using 532 and 870 nm data were as large as 2.5 for smoke with single scattering albedos near unity. The spectral variation in optical properties provides insight into the differentiation of aerosols from mobile or industrial sources versus those from biomass burning. Optical properties of biomass smokes could be classified by general fuel type such as flowering shrubs versus pine needle litter.
George P C Salmond - One of the best experts on this subject based on the ideXlab platform.
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environmental potassium regulates bacterial flotation antibiotic production and turgor pressure in serratia through the trkh transporter
Environmental Microbiology, 2019Co-Authors: Alex Quinteroyanes, Rita E Monson, George P C SalmondAbstract:: Serratia sp. strain ATCC 39006 (S39006) can float in aqueous environments due to natural production of gas vesicles (GVs). Expression of genes for GV morphogenesis is stimulated in low oxygen conditions, thereby enabling migration to the air-liquid interface. Quorum sensing (via SmaI and SmaR) and transcriptional and post-transcriptional regulators, including RbsR and RsmA, respectively, connect the control of cell buoyancy, motility and secondary metabolism. Here, we define a new pleiotropic regulator found in screens of GV mutants. A mutation in the gene trkH, encoding a potassium transporter, caused upregulation of GV formation, flotation, and the prodigiosin antibiotic, and downregulation of flagellar motility. Pressure Nephelometry revealed that the mutation in trkH affected cell turgor pressure. Our results show that osmotic change is an important physiological parameter modulating cell buoyancy and antimicrobial production in S39006, in response to environmental potassium levels.
K Lewis - One of the best experts on this subject based on the ideXlab platform.
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strong spectral variation of biomass smoke light absorption and single scattering albedo observed with a novel dual wavelength photoacoustic instrument
Journal of Geophysical Research, 2008Co-Authors: K Lewis, W P Arnott, Hans Moosmuller, Cyle WoldAbstract:[1] A dual-wavelength photoacoustic instrument operating at 405 and 870 nm was used during the 2006 Fire Lab at Missoula Experiment to measure light scattering and absorption by smoke from the combustion of a variety of biomass fuels. Simultaneous measurements of aerosol light scattering by reciprocal Nephelometry within the instrument's acoustic resonator accompany photoacoustic aerosol light absorption measurements. Single scattering albedo values at 405 nm ranging from 0.37 to 0.95 were measured for different fuel types, and the spectral dependence of absorption was quantified using the Angstrom exponent of absorption. An absorption Angstrom exponent near unity is commonly observed for motor vehicle emission-generated black carbon aerosol. For biomass smoke, Angstrom exponents as high as 3.5 were found in association with smoke having single scattering albedo near unity. The measurements strongly suggest that light-absorbing organic material is present in wood smoke. A second single-wavelength photoacoustic instrument with reciprocal Nephelometry was used to quantify aerosol scattering and absorption at 532 nm. Absorption Angstrom exponents calculated using 532 and 870 nm data were as large as 2.5 for smoke with single scattering albedos near unity. The spectral variation in optical properties provides insight into the differentiation of aerosols from mobile or industrial sources versus those from biomass burning. Optical properties of biomass smokes could be classified by general fuel type such as flowering shrubs versus pine needle litter.
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strong spectral variation of biomass smoke light absorption and single scattering albedo observed with a novel dual wavelength photoacoustic instrument
Journal of Geophysical Research, 2008Co-Authors: K Lewis, W P Arnott, Hans Moosmuller, Cyle WoldAbstract:[1] A dual-wavelength photoacoustic instrument operating at 405 and 870 nm was used during the 2006 Fire Lab at Missoula Experiment to measure light scattering and absorption by smoke from the combustion of a variety of biomass fuels. Simultaneous measurements of aerosol light scattering by reciprocal Nephelometry within the instrument's acoustic resonator accompany photoacoustic aerosol light absorption measurements. Single scattering albedo values at 405 nm ranging from 0.37 to 0.95 were measured for different fuel types, and the spectral dependence of absorption was quantified using the Angstrom exponent of absorption. An absorption Angstrom exponent near unity is commonly observed for motor vehicle emission-generated black carbon aerosol. For biomass smoke, Angstrom exponents as high as 3.5 were found in association with smoke having single scattering albedo near unity. The measurements strongly suggest that light-absorbing organic material is present in wood smoke. A second single-wavelength photoacoustic instrument with reciprocal Nephelometry was used to quantify aerosol scattering and absorption at 532 nm. Absorption Angstrom exponents calculated using 532 and 870 nm data were as large as 2.5 for smoke with single scattering albedos near unity. The spectral variation in optical properties provides insight into the differentiation of aerosols from mobile or industrial sources versus those from biomass burning. Optical properties of biomass smokes could be classified by general fuel type such as flowering shrubs versus pine needle litter.
Alex Quinteroyanes - One of the best experts on this subject based on the ideXlab platform.
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environmental potassium regulates bacterial flotation antibiotic production and turgor pressure in serratia through the trkh transporter
Environmental Microbiology, 2019Co-Authors: Alex Quinteroyanes, Rita E Monson, George P C SalmondAbstract:: Serratia sp. strain ATCC 39006 (S39006) can float in aqueous environments due to natural production of gas vesicles (GVs). Expression of genes for GV morphogenesis is stimulated in low oxygen conditions, thereby enabling migration to the air-liquid interface. Quorum sensing (via SmaI and SmaR) and transcriptional and post-transcriptional regulators, including RbsR and RsmA, respectively, connect the control of cell buoyancy, motility and secondary metabolism. Here, we define a new pleiotropic regulator found in screens of GV mutants. A mutation in the gene trkH, encoding a potassium transporter, caused upregulation of GV formation, flotation, and the prodigiosin antibiotic, and downregulation of flagellar motility. Pressure Nephelometry revealed that the mutation in trkH affected cell turgor pressure. Our results show that osmotic change is an important physiological parameter modulating cell buoyancy and antimicrobial production in S39006, in response to environmental potassium levels.