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José María Monserrat - One of the best experts on this subject based on the ideXlab platform.
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Toxicity of fullerene and nanosilver nanomaterials against bacteria associated to the body surface of the estuarine worm Laeonereis acuta (Polychaeta, Nereididae).
Marine environmental research, 2014Co-Authors: Lucas Freitas Cordeiro, Bianca Fell Marques, Luiza Wilges Kist, Maurício Reis Bogo, Gerardo López, Gisela Pagano, Irene Clemes Külkamp-guerreiro, José María MonserratAbstract:This study analyzed the growth and biochemical responses of six bacterial colonies isolated from the mucus of the estuarine polychaeta Laeonereis acuta (Nereididae) after exposure to a water suspension of fullerene (nC60) and nanosilver (nAg) separately (0.01; 0.10; and 1.00 mg/L) and together (0.01; 0.10; and 1.00 mg/L of nanosilver and 1.00 mg/L of fullerene added to each nAg concentration). Exposures were performed in darkness during 24 h and then samples were taken from the worms and inoculated on agar during 24 h to analyze colonies growth. After this the material was analyzed biochemically. Colonies growth (tested by wet biomass weight) was inhibited at 0.01 and 0.10 mg/L of nAg and 0.01 and 0.10 mg/L nAg + constant 1.00 mg/L of nC60 (p
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Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae)
Environmental Science and Pollution Research, 2011Co-Authors: Juliane Ventura-lima, Patrícia Baptista Ramos, Daniele Fattorini, Francesco Regoli, Luis Ferraz, Leandro M. De Carvalho, José María MonserratAbstract:Introduction This study aimed to analyze antioxidant responses and oxidative damage induced by two inorganic forms of arsenic (As; As^III and As^V) in an estuarine polychaete species, Laeonereis acuta (Nereididae). The capacity of arsenic biotransformation was also evaluated through the methylation process considering the activity of a key enzyme involved in the metabolization process. Materials and methods Worms were exposed to 50 μg (As^III or As^V)/l during 2 or 7 days, plus a control group. Endpoints analyzed included concentration of reactive oxygen species (ROS), activities of antioxidant enzymes such as glutathione reductase (GR), total glutathione- S -transferase (GST), and omega isoform (GST Ω), glucose-6-phosphate deshydrogenase (G6PDH), levels of the antioxidant glutathione (GSH), and lipid peroxides concentration (TBARS). Results and discussion Results showed: (1) GR inhibition after 2-day exposure to both As forms ( p
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Accumulation, biotransformation, and biochemical responses after exposure to arsenite and arsenate in the estuarine polychaete Laeonereis acuta (Nereididae).
Environmental science and pollution research international, 2011Co-Authors: Juliane Ventura-lima, Patrícia Baptista Ramos, Daniele Fattorini, Francesco Regoli, Luis Ferraz, Leandro M. De Carvalho, José María MonserratAbstract:Introduction This study aimed to analyze antioxidant responses and oxidative damage induced by two inorganic forms of arsenic (As; AsIII and AsV) in an estuarine polychaete species, Laeonereis acuta (Nereididae). The capacity of arsenic biotransformation was also evaluated through the methylation process considering the activity of a key enzyme involved in the metabolization process.
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biomonitoring of antioxidant and oxidative stress responses in perinereis gualpensis polychaeta Nereididae in chilean estuarine regions under different anthropogenic pressure
Ecotoxicology and Environmental Safety, 2010Co-Authors: Mauricio Diazjaramillo, J.l. Ferreira, L.l. Amado, M.r. Retamal, Roberto Urrutia, C. Bertrán, Ricardo Barra, Juliane Venturalima, Atila Clivea Martins, José María MonserratAbstract:This study aimed to analyze oxidative stress parameters, including levels of the antioxidant glutathione (GSH), activity of glutamate-cysteine ligase (GCL) and glutathione-S-transferase (GST), total antioxidant capacity and protein oxidation, in the polychaete Perinereis gualpensis (Nereididae) collected from the Biobio, Itata, Valdivia and Lingue estuaries in Chile, which present different degrees of anthropogenic pressure. Sampling sites were characterized considering a geographic information system and the physicochemical characteristics of water and sediment. Significant differences (p<0.05) were observed between the sampling sites for most of the responses (GSH, GCL, GST and antioxidant capacity), mainly related to human activities such as agriculture, industry, among others. Multivariate correlation analysis indicates a certain relationship of antioxidant responses with human activities, salinity, and worm weight, this last employed to standardize GST and antioxidant capacity. These results clearly indicate biomarker responses in P. gualpensis in Biobio and Valdivia estuaries, the more affected by human activities.
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Biomonitoring of antioxidant and oxidative stress responses in Perinereis gualpensis (Polychaeta: Nereididae) in Chilean estuarine regions under different anthropogenic pressure.
Ecotoxicology and environmental safety, 2010Co-Authors: Mauricio Díaz-jaramillo, Juliane Ventura-lima, J.l. Ferreira, L.l. Amado, Átila Clivea Da Silva Martins, M.r. Retamal, Roberto Urrutia, C. Bertrán, Ricardo Barra, José María MonserratAbstract:This study aimed to analyze oxidative stress parameters, including levels of the antioxidant glutathione (GSH), activity of glutamate-cysteine ligase (GCL) and glutathione-S-transferase (GST), total antioxidant capacity and protein oxidation, in the polychaete Perinereis gualpensis (Nereididae) collected from the Biobio, Itata, Valdivia and Lingue estuaries in Chile, which present different degrees of anthropogenic pressure. Sampling sites were characterized considering a geographic information system and the physicochemical characteristics of water and sediment. Significant differences (p
Won Kim - One of the best experts on this subject based on the ideXlab platform.
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Determination of complete mitogenome sequence for Eastern Asian population of Cheilonereis cyclurus (Harrington, 1897) (Polychaeta: Nereididae)
Mitochondrial DNA Part B, 2017Co-Authors: Taeseo Park, Sang-hwa Lee, Myung-hwa Shin, Won KimAbstract:Complete mitogenome sequence for eastern Asian population of Cheilonereis cyclurus (Polychaeta: Nereididae) was determined for the first time. The length of circular genome of C. cyclurus is 14,917...
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complete mitochondrial genome sequence of the giant mud worm paraleonnates uschakovi khlebovich wu 1962 polychaeta Nereididae
Mitochondrial DNA Part B, 2016Co-Authors: Taeseo Park, Sang-hwa Lee, Won KimAbstract:Complete mitochondrial genome sequence of the giant mud worm Paraleonnates uschakovi (Polychaeta: Nereididae) was determined in this study for the first time. The mitogenome of P. uschakovi is 15,5...
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Complete mitochondrial genome sequence of the giant mud worm Paraleonnates uschakovi Khlebovich & Wu, 1962 (Polychaeta: Nereididae)
Mitochondrial DNA Part B, 2016Co-Authors: Taeseo Park, Sang-hwa Lee, Won KimAbstract:Complete mitochondrial genome sequence of the giant mud worm Paraleonnates uschakovi (Polychaeta: Nereididae) was determined in this study for the first time. The mitogenome of P. uschakovi is 15,5...
Karl Jürss - One of the best experts on this subject based on the ideXlab platform.
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Genetic differentiation in Hediste diversicolor (Polychaeta: Nereididae) for the North Sea and the Baltic Sea
Marine Biology, 1997Co-Authors: M. Röhner, Ralf Bastrop, Karl JürssAbstract:Genetic differentiation between North Sea and Baltic Sea Hediste diversicolor (O.F. Muller, 1776) (Polychaeta: Nereididae) populations was studied by allozyme electrophoresis on starch gel. Thirteen loci were analyzed in eight populations. The level of genetic variation was very low (mean H o = 0.000 to 0.015). Differentiation between H. diversicolor populations is quite high (F ST = 0.892) and reflected by three enzyme loci (MDH-I*, MDH-II*, IDH-I*). The reduced gene flow (N m
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genetic differentiation in hediste diversicolor polychaeta Nereididae for the north sea and the baltic sea
Marine Biology, 1997Co-Authors: M. Röhner, Ralf Bastrop, Karl JürssAbstract:Genetic differentiation between North Sea and Baltic Sea Hediste diversicolor (O.F. Muller, 1776) (Polychaeta: Nereididae) populations was studied by allozyme electrophoresis on starch gel. Thirteen loci were analyzed in eight populations. The level of genetic variation was very low (mean H o = 0.000 to 0.015). Differentiation between H. diversicolor populations is quite high (F ST = 0.892) and reflected by three enzyme loci (MDH-I*, MDH-II*, IDH-I*). The reduced gene flow (N m<1) may be explained by the limited dispersal capacity of the species. Regardless of whether found in the North Sea or Baltic Sea, there appear to be two different genetic types which are parapatric or sympatric in some places. The two types hybridize at three localities, but no signs of hybridization have been found at one (Tallinn).
Detlev Arendt - One of the best experts on this subject based on the ideXlab platform.
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the normal development of platynereis dumerilii Nereididae annelida
Frontiers in Zoology, 2010Co-Authors: Antje H L Fischer, Thorsten Henrich, Detlev ArendtAbstract:The polychaete annelid Platynereis dumerilii is an emerging model organism for the study of molecular developmental processes, evolution, neurobiology and marine biology. Annelids belong to the Lophotrochozoa, the so far understudied third major branch of bilaterian animals besides deuterostomes and ecdysozoans. P. dumerilii has proven highly relevant to explore ancient bilaterian conditions via comparison to the deuterostomes, because it has accumulated less evolutionary change than conventional ecdysozoan models. Previous staging was mainly referring to hours post fertilization but did not allow matching stages between studies performed at (even slightly) different temperatures. To overcome this, and to provide a first comprehensive description of P. dumerilii normal development, a temperature-independent staging system is needed. Platynereis dumerilii normal development is subdivided into 16 stages, starting with the zygote and ending with the death of the mature worms after delivering their gametes. The stages described can be easily identified by conventional light microscopy or even by dissecting scope. Developmental landmarks such as the beginning of phototaxis, the visibility of the stomodeal opening and of the chaetae, the first occurrence of the ciliary bands, the formation of the parapodia, the extension of antennae and cirri, the onset of feeding and other characteristics are used to define different developmental stages. The morphology of all larval stages as well as of juveniles and adults is documented by light microscopy. We also provide an overview of important steps in the development of the nervous system and of the musculature, using fluorescent labeling techniques and confocal laser-scanning microscopy. Timing of each developmental stage refers to hours post fertilization at 18 ± 0.1°C. For comparison, we determined the pace of development of larvae raised at 14°C, 16°C, 20°C, 25°C, 28°C and 30°C. A staging ontology representing the comprehensive list of developmental stages of P. dumerilii is available online. Our atlas of Platynereis dumerilii normal development represents an important resource for the growing Platynereis community and can also be applied to other nereidid annelids.
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The normal development of Platynereis dumerilii (Nereididae, Annelida)
Frontiers in Zoology, 2010Co-Authors: Antje H L Fischer, Thorsten Henrich, Detlev ArendtAbstract:Background The polychaete annelid Platynereis dumerilii is an emerging model organism for the study of molecular developmental processes, evolution, neurobiology and marine biology. Annelids belong to the Lophotrochozoa, the so far understudied third major branch of bilaterian animals besides deuterostomes and ecdysozoans. P. dumerilii has proven highly relevant to explore ancient bilaterian conditions via comparison to the deuterostomes, because it has accumulated less evolutionary change than conventional ecdysozoan models. Previous staging was mainly referring to hours post fertilization but did not allow matching stages between studies performed at (even slightly) different temperatures. To overcome this, and to provide a first comprehensive description of P. dumerilii normal development, a temperature-independent staging system is needed. Results Platynereis dumerilii normal development is subdivided into 16 stages, starting with the zygote and ending with the death of the mature worms after delivering their gametes. The stages described can be easily identified by conventional light microscopy or even by dissecting scope. Developmental landmarks such as the beginning of phototaxis, the visibility of the stomodeal opening and of the chaetae, the first occurrence of the ciliary bands, the formation of the parapodia, the extension of antennae and cirri, the onset of feeding and other characteristics are used to define different developmental stages. The morphology of all larval stages as well as of juveniles and adults is documented by light microscopy. We also provide an overview of important steps in the development of the nervous system and of the musculature, using fluorescent labeling techniques and confocal laser-scanning microscopy. Timing of each developmental stage refers to hours post fertilization at 18 ± 0.1°C. For comparison, we determined the pace of development of larvae raised at 14°C, 16°C, 20°C, 25°C, 28°C and 30°C. A staging ontology representing the comprehensive list of developmental stages of P. dumerilii is available online. Conclusions Our atlas of Platynereis dumerilii normal development represents an important resource for the growing Platynereis community and can also be applied to other nereidid annelids.
Leonid P Nezlin - One of the best experts on this subject based on the ideXlab platform.
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Development of the nervous system in Platynereis dumerilii (Nereididae, Annelida)
Frontiers in Zoology, 2017Co-Authors: Viktor V Starunov, Elena E Voronezhskaya, Leonid P NezlinAbstract:Background The structure and development of the nervous system in Lophotrochozoa has long been recognized as one of the most important subjects for phylogenetic and evolutionary discussion. Many recent papers have presented comprehensive data on the structure and development of catecholaminergic, serotonergic and FMRFamidergic parts of the nervous system. However, relatively few papers contain detailed descriptions of the nervous system in Annelida, one of the largest taxa of Lophotrochozoa. The polychaete species Platynereis dumerilii has recently become one of the more popular model animals in evolutionary and developmental biology. The goal of the present study was to provide a detailed description of its neuronal development. The data obtained will contribute to a better understanding of the basic features of neuronal development in polychaetes. Results We have studied the development of the nervous system in P. dumerilii utilizing histo- and immunochemical labelling of catecholamines, serotonin, FMRFamide related peptides, and acetylated tubulin. The first neuron differentiates at the posterior extremity of the protrochophore, reacts to the antibodies against both serotonin and FMRFamide. Then its fibres run forwards along the ventral side. Soon, more neurons appear at the apical extreme, and their basal neurites form the basel structure of the developing brain (cerebral neuropil and circumesophageal connectives). Initial development of the nervous system starts in two rudiments: anterior and posterior. At the nectochaete stage, segmental ganglia start to differentiate in the anterior-to-posterior direction, and the first structures of the stomatogastric and peripheral nervous system appear. All connectives including the unpaired ventral cord develop from initially paired nerves. Conclusions We present a detailed description of Platynereis dumerilii neuronal development based on anti-acetylated tubulin, serotonin, and FMRFamide-like immunostaining as well as catecholamine histofluorescence. The development of the nervous system starts from peripheral pioneer neurons at both the posterior and anterior poles of the larva, and their neurites form a scaffold upon which the adult central nervous system develops. The anterior-to-posterior mode of the ventral ganglia development challenges the primary heteronomy concept. Comparison with the development of Mollusca reveals substantial similarities with early neuronal development in larval Solenogastres.
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development of the nervous system in platynereis dumerilii Nereididae annelida
Frontiers in Zoology, 2017Co-Authors: Viktor V Starunov, Elena E Voronezhskaya, Leonid P NezlinAbstract:The structure and development of the nervous system in Lophotrochozoa has long been recognized as one of the most important subjects for phylogenetic and evolutionary discussion. Many recent papers have presented comprehensive data on the structure and development of catecholaminergic, serotonergic and FMRFamidergic parts of the nervous system. However, relatively few papers contain detailed descriptions of the nervous system in Annelida, one of the largest taxa of Lophotrochozoa. The polychaete species Platynereis dumerilii has recently become one of the more popular model animals in evolutionary and developmental biology. The goal of the present study was to provide a detailed description of its neuronal development. The data obtained will contribute to a better understanding of the basic features of neuronal development in polychaetes. We have studied the development of the nervous system in P. dumerilii utilizing histo- and immunochemical labelling of catecholamines, serotonin, FMRFamide related peptides, and acetylated tubulin. The first neuron differentiates at the posterior extremity of the protrochophore, reacts to the antibodies against both serotonin and FMRFamide. Then its fibres run forwards along the ventral side. Soon, more neurons appear at the apical extreme, and their basal neurites form the basel structure of the developing brain (cerebral neuropil and circumesophageal connectives). Initial development of the nervous system starts in two rudiments: anterior and posterior. At the nectochaete stage, segmental ganglia start to differentiate in the anterior-to-posterior direction, and the first structures of the stomatogastric and peripheral nervous system appear. All connectives including the unpaired ventral cord develop from initially paired nerves. We present a detailed description of Platynereis dumerilii neuronal development based on anti-acetylated tubulin, serotonin, and FMRFamide-like immunostaining as well as catecholamine histofluorescence. The development of the nervous system starts from peripheral pioneer neurons at both the posterior and anterior poles of the larva, and their neurites form a scaffold upon which the adult central nervous system develops. The anterior-to-posterior mode of the ventral ganglia development challenges the primary heteronomy concept. Comparison with the development of Mollusca reveals substantial similarities with early neuronal development in larval Solenogastres.
