The Experts below are selected from a list of 57465 Experts worldwide ranked by ideXlab platform
Azize Alayli - One of the best experts on this subject based on the ideXlab platform.
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The effects of MgS nanoparticles-Cisplatin-bio-conjugate on SH-SY5Y Neuroblastoma Cell line
Molecular Biology Reports, 2020Co-Authors: Ozge Balpinar Nalci, Hayrunnisa Nadaroglu, Ahmet Hacimuftuoglu, Sidika Genc, Azize AlayliAbstract:Magnesium sulfide nanoparticles (MgS NPs) is a nanomaterial that has an important place in diagnosis, treatment, diagnosis, and drug delivery systems. Neuroblastoma, a type of brain cancer, is an extremely difficult cancer to treat with today’s treatment options. This study was carried out to determine the cytotoxic, oxidant, and antioxidant effects on the Neuroblastoma cancer line (SH-SY5Y Cell line) along with the green synthesis and characterization of MgS NPs structures. MgS NPs were synthesized by green synthesis using Na_2S and Punica granatum , a cleaner method for toxic effects, and characterized using Scanning Electron Microscopy, Fourier Transform Infrared spectroscopy, X-Ray diffraction methods. In Cell culture, SH-SY5Y Cells were grown in a suitable nutrient medium under favorable conditions. Five different doses of MgS NPs (10, 25, 50, 75, and 100 µg/mL) were applied to the Cell line for 24 h. The analysis of the MgS NPs applications was performed with MTT cytotoxicity test and total oxidant and total antioxidant tests. According to the data obtained, 75 μg/mL MgS NPs application decreased cancer Cell viability up to 48.54%. MgS NPs exhibited a dose-dependent effect on the SH-SY5Y Cell line. Also, it was determined that MgS NPs increased oxidant activity in Neuroblastoma Cells, which was compatible with the cytotoxicity test. As a result, MgS NPs exhibited an effective activity on the Neuroblastoma Cell line. It was clearly seen that NPs obtained by green synthesis prevented the related cancer line from proliferating.
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Preparation of CoS nanoparticles-cisplatin bio-conjugates and investigation of their effects on SH-SY5Y Neuroblastoma Cell line
Cytotechnology, 2020Co-Authors: Muhammed Sait Ertugrul, Hayrunnisa Nadaroglu, Ozge Balpinar Nalci, Ahmet Hacimuftuoglu, Azize AlayliAbstract:Neuroblastoma is one of the most widely seen under the age of 15 tumors that occur in the adrenal medulla and sympathetic ganglia. Cisplatin, an antineoplastic drug, is a Platinum-based compound and is known to inhibit the proliferation of Neuroblastoma Cells. Effective applications of nanoparticles in biomedical areas such as biomolecular, antimicrobial detection and diagnosis, tissue engineering, theranostics, biomarking, drug delivery, and anti-cancer have been investigated in many studies. This study aims to prepare the bioconjugates of CoS (cobalt sulfide) nanoparticles (NPs) with cisplatin combination groups and to evaluate their effects on the Neuroblastoma Cell line. Nanoparticle synthesis was done using the green synthesis technique using Punica granatum plant extract. The size and shape of CoS NPs were characterized by SEM, FT-IR, and XRD. Zeta potential was confirmed by the DLS study. For this purpose, the SH-SY5Y Neuroblastoma Cell line was cultured in a suitable Cell culture medium. Cisplatin 5 µg and different concentrations (Cisplatin + CoS NPs bioconjugates (5, 10, 25, 50, 75 μg) doses were applied to SH-SY5Y Neuroblastoma Cell lines for 24 h. TAC, TOS and MTT tests were performed 24 h after the application. According to the MTT test results, cisplatin and CoS NP combinations reduced the proliferation of Neuroblastoma Cells by 78 to 57% compared to the cisplatin control. From the findings obtained; the most effective Bio-conjugate group was Cisplatin 5 μg/mL + CoS 75 μg/mL.
Angelika Eggert - One of the best experts on this subject based on the ideXlab platform.
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temporal proteomics of ngf trka signaling identifies an inhibitory role for the e3 ligase cbl b in Neuroblastoma Cell differentiation
Science Signaling, 2015Co-Authors: Kristina B Emdal, Annakathrine Pedersen, Dorte B Bekkerjensen, Kalliopi Tsafou, Heiko Horn, Sven Lindner, Johannes H Schulte, Angelika EggertAbstract:SH-SY5Y Neuroblastoma Cells respond to nerve growth factor (NGF)–mediated activation of the tropomyosin-related kinase A (TrkA) with neurite outgrowth, thereby providing a model to study neuronal differentiation. We performed a time-resolved analysis of NGF-TrkA signaling in Neuroblastoma Cells using mass spectrometry–based quantitative proteomics. The combination of interactome, phosphoproteome, and proteome data provided temporal insights into the molecular events downstream of NGF binding to TrkA. We showed that upon NGF stimulation, TrkA recruits the E3 ubiquitin ligase Cbl-b, which then becomes phosphorylated and ubiquitylated and decreases in abundance. We also found that recruitment of Cbl-b promotes TrkA ubiquitylation and degradation. Furthermore, the amount of phosphorylation of the kinase ERK and neurite outgrowth increased upon Cbl-b depletion in several Neuroblastoma Cell lines. Our findings suggest that Cbl-b limits NGF-TrkA signaling to control the length of neurites.
Olga Boyd - One of the best experts on this subject based on the ideXlab platform.
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systems genomics evaluation of the sh sy5y Neuroblastoma Cell line as a model for parkinson s disease
BMC Genomics, 2014Co-Authors: Abhimanyu Krishna, Maria Biryukov, Christophe Trefois, Paul Antony, Rene Hussong, Jake Lin, Merja Heinaniemi, Gustavo Glusman, Sandra Koglsberger, Olga BoydAbstract:The human Neuroblastoma Cell line, SH-SY5Y, is a commonly used Cell line in studies related to neurotoxicity, oxidative stress, and neurodegenerative diseases. Although this Cell line is often used as a Cellular model for Parkinson’s disease, the relevance of this Cellular model in the context of Parkinson’s disease (PD) and other neurodegenerative diseases has not yet been systematically evaluated. We have used a systems genomics approach to characterize the SH-SY5Y Cell line using whole-genome sequencing to determine the genetic content of the Cell line and used transcriptomics and proteomics data to determine molecular correlations. Further, we integrated genomic variants using a network analysis approach to evaluate the suitability of the SH-SY5Y Cell line for perturbation experiments in the context of neurodegenerative diseases, including PD. The systems genomics approach showed consistency across different biological levels (DNA, RNA and protein concentrations). Most of the genes belonging to the major Parkinson’s disease pathways and modules were intact in the SH-SY5Y genome. Specifically, each analysed gene related to PD has at least one intact copy in SH-SY5Y. The disease-specific network analysis approach ranked the genetic integrity of SH-SY5Y as higher for PD than for Alzheimer’s disease but lower than for Huntington’s disease and Amyotrophic Lateral Sclerosis for loss of function perturbation experiments.
Roberto Maggi - One of the best experts on this subject based on the ideXlab platform.
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retinoic acid reduces human Neuroblastoma Cell migration and invasiveness effects on dcx lis1 neurofilaments 68 and vimentin expression
BMC Cancer, 2008Co-Authors: Elio Messi, Maria Carolina Florian, Claudio Caccia, M Zanisi, Roberto MaggiAbstract:Background Neuroblastoma is a severe pediatric tumor, histologically characterised by a variety of Cellular phenotypes. One of the pharmacological approaches to Neuroblastoma is the treatment with retinoic acid. The mechanism of action of retinoic acid is still unclear, and the development of resistance to this differentiating agent is a great therapy problem. Doublecortin, a microtubule-associated protein involved in neuronal migration, has recently been proposed as a molecular marker for the detection of minimal residual disease in human Neuroblastoma. Nevertheless, no information is available on the expression of doublecortin in the different Cell-types composing human Neuroblastoma, its correlation with Neuroblastoma Cell motility and invasiveness, and the possible modulations exerted by retinoic acid treatment.
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Effects and metabolism of steroid hormones in human Neuroblastoma Cells.
Steroids, 1998Co-Authors: Roberto Maggi, Angelo Poletti, L.a. Casulari, Federica Pimpinelli, Flavio Piva, Maria R. Zanisi, Luciano MartiniAbstract:Abstract The development of the central nervous system is influenced by sex steroids and by their metabolites. However, little information on the possible effects of steroid hormones on Neuroblastoma Cells is available. Human Neuroblastoma Cell lines have been used as a model of human neuroblasts in vitro to study the metabolism of steroid hormones; in addition, the effects of steroids and steroid antagonists on Neuroblastoma Cell growth have also been investigated. The results obtained show that SH-SY5Y human Neuroblastoma Cells may actively metabolize testosterone and progesterone to their respective 5α-reduced metabolites and that differentiation of Neuroblastoma Cells is paralleled by a significant increase in expression of the type-1 5α-reductase and of the formation of steroid metabolites. All these data are suggestive of a potential role of steroid 5α-reduced metabolites in the biology of Neuroblastoma Cells. Studies performed to analyze the role of steroid hormones on Neuroblastoma Cell proliferation show that progesterone at low doses may induce minor stimulation, and at higher doses, a toxic effect on the Neuroblastoma Cell line SK-N-SH is seen. Moreover, the antiprogestin 17β-hydroxy-11β-(4-dimethylamino-phenyl-1)-17-(prop-1-ynyl)estra-4,9-dien-3-one (RU486) decreases the proliferation of these Cells in a dose-dependent manner. The effect of RU486 is not antagonized by either progesterone or dexamethasone, a result that seems to exclude the action of RU486 via classic intraCellular steroid hormone receptors.
Graeme Henderson - One of the best experts on this subject based on the ideXlab platform.
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the effect of nociceptin on ca2 channel current and intraCellular ca2 in the sh sy5y human Neuroblastoma Cell line
British Journal of Pharmacology, 1996Co-Authors: Mark Connor, Alison Yoe, Graeme HendersonAbstract:The human Neuroblastoma Cell line SH-SY5Y expresses the 'orphan' opioid receptor (ORL1). We have demonstrated that nociceptin, the putative endogenous ligand for ORL1, produces a concentration-dependent inhibition of the N-type calcium channel current in these Cells (IC50 42 nM). In addition, in the presence of carbachol, nociceptin increased the intraCellular concentration of Ca2+ (EC50 60 nM). Both effects of nociceptin were blocked by pertussis toxin pretreatment but not by the opioid antagonists CTAP (1 microM), naltrindole (1 microM) and naloxone (10 microM).