The Experts below are selected from a list of 12 Experts worldwide ranked by ideXlab platform
Paola S. Timiras - One of the best experts on this subject based on the ideXlab platform.
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Early Anti-Oxidative and Anti-Proliferative CurcuMin Effects on NeuroglioMa Cells Suggest Therapeutic Targets
Neurochemical Research, 2008Co-Authors: Heena D. Panchal, Karen Vranizan, Chun Y. Lee, John Ngai, Paola S. TimirasAbstract:CurcuMin (diferuloyl), froM the Indian spice turMeric, reduces oxidative daMage and induces apoptosis. Utilizing DNA Microarrays, we have deMonstrated that a low (5 μM) dose of curcuMin added to a Mixture of astrocytes and oligodendrocytes (C6 rat glioMa cells) in culture for 24 and 48 h significantly Modulates gene expression in four priMary pathways: oxidative stress, cell cycle control, and DNA transcription and MetabolisM. Contribution of the pentose phosphate pathway to the pool of NADH upregulates glutathione and activates aldehyde oxidase. We have identified also several new genes, up- or downregulated by curcuMin, naMely, aldo-keto reductase, glucose-6-phosphate dehydrogenase, and aldehyde oxidase that protect against oxidative stress. The identification of several new cell cycle control genes, including the apoptosis-related Protein (pirin) and insulin-like growth factor (IGF), and of the NeurofilaMent M Protein involved in neurogenesis suggests that curcuMin May have applicability in the treatMent of a spectruM of neurodegenerative diseases.
Heena D. Panchal - One of the best experts on this subject based on the ideXlab platform.
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Early Anti-Oxidative and Anti-Proliferative CurcuMin Effects on NeuroglioMa Cells Suggest Therapeutic Targets
Neurochemical Research, 2008Co-Authors: Heena D. Panchal, Karen Vranizan, Chun Y. Lee, John Ngai, Paola S. TimirasAbstract:CurcuMin (diferuloyl), froM the Indian spice turMeric, reduces oxidative daMage and induces apoptosis. Utilizing DNA Microarrays, we have deMonstrated that a low (5 μM) dose of curcuMin added to a Mixture of astrocytes and oligodendrocytes (C6 rat glioMa cells) in culture for 24 and 48 h significantly Modulates gene expression in four priMary pathways: oxidative stress, cell cycle control, and DNA transcription and MetabolisM. Contribution of the pentose phosphate pathway to the pool of NADH upregulates glutathione and activates aldehyde oxidase. We have identified also several new genes, up- or downregulated by curcuMin, naMely, aldo-keto reductase, glucose-6-phosphate dehydrogenase, and aldehyde oxidase that protect against oxidative stress. The identification of several new cell cycle control genes, including the apoptosis-related Protein (pirin) and insulin-like growth factor (IGF), and of the NeurofilaMent M Protein involved in neurogenesis suggests that curcuMin May have applicability in the treatMent of a spectruM of neurodegenerative diseases.
Nora I. Perrone-bizzozero - One of the best experts on this subject based on the ideXlab platform.
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Overexpression of HuD, but not of its truncated forM HuD I+II, proMotes GAP-43 gene expression and neurite outgrowth in PC12 cells in the absence of nerve growth factor.
Journal of neurochemistry, 2002Co-Authors: Kim D. Anderson, M. Morin, Andrea Beckel-mitchener, Charlotte D. Mobarak, Rachael L. Neve, Henry Furneaux, Richard W. Burry, Nora I. Perrone-bizzozeroAbstract:We have previously shown that the RNA-binding Protein HuD binds to a regulatory eleMent in the growth-associated Protein (GAP)-43 MRNA and that this interaction involves its first two RNA recognition Motifs (RRMs). In this study, we investigated the functional significance of this interaction by overexpression of huMan HuD Protein (pcHuD) or its truncated forM lacking the third RRM (pcHuD I+II) in PC12 cells. Morphological analysis revealed that pcHuD cells extended short neurites containing GAP-43-positive growth cones in the absence of nerve growth factor (NGF). These processes also contained tubulin and F-actin filaMents but were not stained with antibodies against NeurofilaMent M Protein. In correlation with this phenotype, pcHuD cells contained higher levels of GAP-43 without changes in levels of other NGF-induced Proteins, such as SNAP-25 and tau. In MRNA decay studies, HuD stabilized the GAP-43 MRNA, whereas HuD I+II did not have any effect either on GAP-43 MRNA stability or on the levels of GAP-43 Protein. Likewise, pcHuD I+II cells showed no spontaneous neurite outgrowth and deficient outgrowth in response to NGF. Our results indicate that HuD is sufficient to increase GAP-43 gene expression and neurite outgrowth in the absence of NGF and that the third RRM in the Protein is critical for this function.
Chun Y. Lee - One of the best experts on this subject based on the ideXlab platform.
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Early Anti-Oxidative and Anti-Proliferative CurcuMin Effects on NeuroglioMa Cells Suggest Therapeutic Targets
Neurochemical Research, 2008Co-Authors: Heena D. Panchal, Karen Vranizan, Chun Y. Lee, John Ngai, Paola S. TimirasAbstract:CurcuMin (diferuloyl), froM the Indian spice turMeric, reduces oxidative daMage and induces apoptosis. Utilizing DNA Microarrays, we have deMonstrated that a low (5 μM) dose of curcuMin added to a Mixture of astrocytes and oligodendrocytes (C6 rat glioMa cells) in culture for 24 and 48 h significantly Modulates gene expression in four priMary pathways: oxidative stress, cell cycle control, and DNA transcription and MetabolisM. Contribution of the pentose phosphate pathway to the pool of NADH upregulates glutathione and activates aldehyde oxidase. We have identified also several new genes, up- or downregulated by curcuMin, naMely, aldo-keto reductase, glucose-6-phosphate dehydrogenase, and aldehyde oxidase that protect against oxidative stress. The identification of several new cell cycle control genes, including the apoptosis-related Protein (pirin) and insulin-like growth factor (IGF), and of the NeurofilaMent M Protein involved in neurogenesis suggests that curcuMin May have applicability in the treatMent of a spectruM of neurodegenerative diseases.
John Ngai - One of the best experts on this subject based on the ideXlab platform.
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Early Anti-Oxidative and Anti-Proliferative CurcuMin Effects on NeuroglioMa Cells Suggest Therapeutic Targets
Neurochemical Research, 2008Co-Authors: Heena D. Panchal, Karen Vranizan, Chun Y. Lee, John Ngai, Paola S. TimirasAbstract:CurcuMin (diferuloyl), froM the Indian spice turMeric, reduces oxidative daMage and induces apoptosis. Utilizing DNA Microarrays, we have deMonstrated that a low (5 μM) dose of curcuMin added to a Mixture of astrocytes and oligodendrocytes (C6 rat glioMa cells) in culture for 24 and 48 h significantly Modulates gene expression in four priMary pathways: oxidative stress, cell cycle control, and DNA transcription and MetabolisM. Contribution of the pentose phosphate pathway to the pool of NADH upregulates glutathione and activates aldehyde oxidase. We have identified also several new genes, up- or downregulated by curcuMin, naMely, aldo-keto reductase, glucose-6-phosphate dehydrogenase, and aldehyde oxidase that protect against oxidative stress. The identification of several new cell cycle control genes, including the apoptosis-related Protein (pirin) and insulin-like growth factor (IGF), and of the NeurofilaMent M Protein involved in neurogenesis suggests that curcuMin May have applicability in the treatMent of a spectruM of neurodegenerative diseases.