The Experts below are selected from a list of 327 Experts worldwide ranked by ideXlab platform
Carlos A Saura - One of the best experts on this subject based on the ideXlab platform.
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crtc1 mediates preferential transcription at Neuronal Activity regulated cre tata promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parradamas, Laura Rubioferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
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CRTC1 mediates preferential transcription at Neuronal Activity-regulated CRE/TATA promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parra-damas, Laura Rubió-ferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
Jie Shen - One of the best experts on this subject based on the ideXlab platform.
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crtc1 mediates preferential transcription at Neuronal Activity regulated cre tata promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parradamas, Laura Rubioferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
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CRTC1 mediates preferential transcription at Neuronal Activity-regulated CRE/TATA promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parra-damas, Laura Rubió-ferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
Koichi Kawakami - One of the best experts on this subject based on the ideXlab platform.
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Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish.
Methods in Molecular Biology, 2016Co-Authors: Akira Muto, Koichi KawakamiAbstract:Visualization of Neuronal Activity during animal behavior is a critical step in understanding how the brain generates behavior. In the model vertebrate zebrafish, imaging of the brain has been done mostly by using immobilized fish. Here, we describe a novel method to image Neuronal Activity of the larval zebrafish brain during prey capture behavior. We expressed a genetically encoded fluorescent calcium indicator, GCaMP, in the optic tectum of the midbrain using the Gal4-UAS system. Tectal Activity was then imaged in unrestrained larvae during prey perception. Since larval zebrafish swim only intermittently, detection of the Neuronal Activity is possible between swimming bouts. Our method makes functional brain imaging under natural behavioral conditions feasible and will greatly benefit the study of Neuronal activities that evoke animal behaviors.
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Real-Time Visualization of Neuronal Activity during Perception
Current Biology, 2013Co-Authors: Akira Muto, Masamichi Ohkura, Gembu Abe, Junichi Nakai, Koichi KawakamiAbstract:To understand how the brain perceives the external world, it is desirable to observe Neuronal Activity in the brain in real time during perception. The zebrafish is a suitable model animal for fluorescence imaging studies to visualize Neuronal Activity because its body is transparent through the embryonic and larval stages. Imaging studies have been carried out to monitor Neuronal Activity in the larval spinal cord and brain using Ca(2+) indicator dyes and DNA-encoded Ca(2+) indicators, such as Cameleon, GFP-aequorin, and GCaMPs. However, temporal and spatial resolution and sensitivity of these tools are still limited, and imaging of brain Activity during perception of a natural object has not yet been demonstrated. Here we demonstrate visualization of Neuronal Activity in the optic tectum of larval zebrafish by genetically expressing the new version of GCaMP. First, we demonstrate Ca(2+) transients in the tectum evoked by a moving spot on a display and identify direction-selective neurons. Second, we show tectal Activity during perception of a natural object, a swimming paramecium, revealing a functional visuotopic map. Finally, we image the tectal responses of a free-swimming larval fish to a paramecium and thereby correlate Neuronal Activity in the brain with prey capture behavior.
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Report Real-Time Visualization of Neuronal Activity during Perception
2013Co-Authors: Akira Muto, Masamichi Ohkura, Gembu Abe, Junichi Nakai, Koichi KawakamiAbstract:Brain Science Institute, Saitama University,255 Shimo-Okubo, Sakura-ku, Saitama 338-8570, JapanSummaryTo understand how the brain perceives the external world,it is desirable to observe Neuronal Activity in the brain inreal time during perception. The zebrafish is a suitablemodel animal for fluorescence imaging studies to visualizeNeuronal Activity because its body is transparent throughthe embryonic and larval stages. Imaging studies havebeen carried out to monitor Neuronal Activity in the larvalspinal cord and brain using Ca
Arnaldo Parradamas - One of the best experts on this subject based on the ideXlab platform.
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crtc1 mediates preferential transcription at Neuronal Activity regulated cre tata promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parradamas, Laura Rubioferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
Arnaldo Parra-damas - One of the best experts on this subject based on the ideXlab platform.
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CRTC1 mediates preferential transcription at Neuronal Activity-regulated CRE/TATA promoters
Scientific Reports, 2017Co-Authors: Arnaldo Parra-damas, Laura Rubió-ferrarons, Jie Shen, Carlos A SauraAbstract:Gene expression mediated by the transcription factor cAMP-responsive element-binding protein (CREB) is essential for a wide range of brain processes. The transcriptional coactivartor CREB-regulated transcription coactivator-1 (CRTC1) is required for efficient induction of CREB target genes during Neuronal Activity. However, the mechanisms regulating induction of specific CREB/CRTC1-dependent genes during Neuronal Activity remain largely unclear. Here, we investigated the molecular mechanisms regulating Activity-dependent gene transcription upon activation of the CREB/CRTC1 signaling pathway in neurons. Depolarization and cAMP signals induce preferential transcription of Activity-dependent genes containing promoters with proximal CRE/TATA sequences, such as c-fos, Dusp1, Nr4a1, Nr4a2 and Ptgs2, but not genes with proximal CRE/TATA-less promoters (e.g. Nr4a3, Presenilin-1 and Presenilin-2). Notably, biochemical and chromatin immunoprecipitation analyses reveal constitutive binding of CREB to target gene promoters in the absence of Neuronal Activity, whereas recruitment of CRTC1 to proximal CRE/TATA promoters depends on Neuronal Activity. Neuronal Activity induces rapid CRTC1 dephosphorylation, nuclear translocation and binding to endogenous CREB. These results indicate that Neuronal Activity induces a preferential binding of CRTC1 to the transcriptional complex in CRE/TATA-containing promoters to engage Activity-dependent transcription in neurons.
