The Experts below are selected from a list of 405 Experts worldwide ranked by ideXlab platform

Robia G Pautler - One of the best experts on this subject based on the ideXlab platform.

  • in vivo trans synaptic Tract tracing from the murine striatum and amygdala utilizing manganese enhanced mri memri
    Magnetic Resonance in Medicine, 2003
    Co-Authors: Robia G Pautler, Raymond Mongeau, Russell E Jacobs
    Abstract:

    Small focal injections of manganese ion (Mn^(2+)) deep within the mouse central nervous system combined with in vivo high-resolution MRI delineate Neuronal Tracts originating from the site of injection. Previous work has shown that Mn^(2+) can be taken up through voltage-gated Ca^(2+) channels, transported along axons, and across synapses. Moreover, Mn^(2+) is a paramagnetic MRI contrast agent, causing positive contrast enhancement in tissues where it has accumulated. These combined properties allow for its use as an effective MRI detectable Neuronal Tract Tracer. Injections of low concentrations of MnCl_2 into either the striatum or amygdala produced significant contrast enhancement along the known Neuronal circuitry. The observed enhancement pattern is different at each injection site and enhancement of the homotopic areas was observed in both cases. Ten days postinjection, the Mn^(2+) had washed out, as evidenced by the absence of positive contrast enhancement within the brain. This methodology allows imaging of Neuronal Tracts long after the injection of the ion because Mn^(2+) concentrates in active neurons and resides for extended periods of time. With appropriate controls, differentiation of subsets of Neuronal pathways associated with behavioral and pharmacological paradigms should be feasible.

  • in vivo Neuronal Tract tracing using manganese enhanced magnetic resonance imaging
    Magnetic Resonance in Medicine, 1998
    Co-Authors: Robia G Pautler, Afonso C Silva, Alan Koretsky
    Abstract:

    Development of efficient imaging techniques to trace neuro nal connections would be very useful. Manganese ion (Mn2+) is an excellent T1 contrast agent for magnetic resonance imaging (MRI). Four reports utilizing radioactive Mn2+ in fish and rat brain indicate that Mn2+ may be useful for tracing Neuronal connections. Therefore, the purpose of this work was to determine if Mn2+ can be used as an in vivo MRI Neuronal Tract Tracer. The results indicate that topical admin istration of MnCI2 solution to the naris of mice as well as to the retinal ganglion cells via intravitreal injection leads to en hancement of contrast along the respective pathways. There fore, application of Mn2+ to neurons allows the use of MRI to visualize Neuronal connections.

Santiago Canals - One of the best experts on this subject based on the ideXlab platform.

  • biocytin derived mri contrast agent for longitudinal brain connectivity studies
    ACS Chemical Neuroscience, 2011
    Co-Authors: Anurag Mishra, Almut Schuz, N K Logothetis, J Engelmann, Michael Beyerlein, Santiago Canals
    Abstract:

    To investigate the connectivity of brain networks noninvasively and dynamically, we have developed a new strategy to functionalize Neuronal Tracers and designed a biocompatible probe that can be visualized in vivo using magnetic resonance imaging (MRI). Furthermore, the multimodal design used allows combined ex vivo studies with microscopic spatial resolution by conventional histochemical techniques. We present data on the functionalization of biocytin, a well-known Neuronal Tract Tracer, and demonstrate the validity of the approach by showing brain networks of cortical connectivity in live rats under MRI, together with the corresponding microscopic details, such as fibers and Neuronal morphology under light microscopy. We further demonstrate that the developed molecule is the first MRI-visible probe to preferentially trace retrograde connections. Our study offers a new platform for the development of multimodal molecular imaging tools of broad interest in neuroscience, that capture in vivo the dynamics of large scale neural networks together with their microscopic characteristics, thereby spanning several organizational levels.

Alan Koretsky - One of the best experts on this subject based on the ideXlab platform.

  • in vivo Neuronal Tract tracing using manganese enhanced magnetic resonance imaging
    Magnetic Resonance in Medicine, 1998
    Co-Authors: Robia G Pautler, Afonso C Silva, Alan Koretsky
    Abstract:

    Development of efficient imaging techniques to trace neuro nal connections would be very useful. Manganese ion (Mn2+) is an excellent T1 contrast agent for magnetic resonance imaging (MRI). Four reports utilizing radioactive Mn2+ in fish and rat brain indicate that Mn2+ may be useful for tracing Neuronal connections. Therefore, the purpose of this work was to determine if Mn2+ can be used as an in vivo MRI Neuronal Tract Tracer. The results indicate that topical admin istration of MnCI2 solution to the naris of mice as well as to the retinal ganglion cells via intravitreal injection leads to en hancement of contrast along the respective pathways. There fore, application of Mn2+ to neurons allows the use of MRI to visualize Neuronal connections.

Russell E Jacobs - One of the best experts on this subject based on the ideXlab platform.

  • in vivo trans synaptic Tract tracing from the murine striatum and amygdala utilizing manganese enhanced mri memri
    Magnetic Resonance in Medicine, 2003
    Co-Authors: Robia G Pautler, Raymond Mongeau, Russell E Jacobs
    Abstract:

    Small focal injections of manganese ion (Mn^(2+)) deep within the mouse central nervous system combined with in vivo high-resolution MRI delineate Neuronal Tracts originating from the site of injection. Previous work has shown that Mn^(2+) can be taken up through voltage-gated Ca^(2+) channels, transported along axons, and across synapses. Moreover, Mn^(2+) is a paramagnetic MRI contrast agent, causing positive contrast enhancement in tissues where it has accumulated. These combined properties allow for its use as an effective MRI detectable Neuronal Tract Tracer. Injections of low concentrations of MnCl_2 into either the striatum or amygdala produced significant contrast enhancement along the known Neuronal circuitry. The observed enhancement pattern is different at each injection site and enhancement of the homotopic areas was observed in both cases. Ten days postinjection, the Mn^(2+) had washed out, as evidenced by the absence of positive contrast enhancement within the brain. This methodology allows imaging of Neuronal Tracts long after the injection of the ion because Mn^(2+) concentrates in active neurons and resides for extended periods of time. With appropriate controls, differentiation of subsets of Neuronal pathways associated with behavioral and pharmacological paradigms should be feasible.

Anurag Mishra - One of the best experts on this subject based on the ideXlab platform.

  • biocytin derived mri contrast agent for longitudinal brain connectivity studies
    ACS Chemical Neuroscience, 2011
    Co-Authors: Anurag Mishra, Almut Schuz, N K Logothetis, J Engelmann, Michael Beyerlein, Santiago Canals
    Abstract:

    To investigate the connectivity of brain networks noninvasively and dynamically, we have developed a new strategy to functionalize Neuronal Tracers and designed a biocompatible probe that can be visualized in vivo using magnetic resonance imaging (MRI). Furthermore, the multimodal design used allows combined ex vivo studies with microscopic spatial resolution by conventional histochemical techniques. We present data on the functionalization of biocytin, a well-known Neuronal Tract Tracer, and demonstrate the validity of the approach by showing brain networks of cortical connectivity in live rats under MRI, together with the corresponding microscopic details, such as fibers and Neuronal morphology under light microscopy. We further demonstrate that the developed molecule is the first MRI-visible probe to preferentially trace retrograde connections. Our study offers a new platform for the development of multimodal molecular imaging tools of broad interest in neuroscience, that capture in vivo the dynamics of large scale neural networks together with their microscopic characteristics, thereby spanning several organizational levels.