The Experts below are selected from a list of 16464 Experts worldwide ranked by ideXlab platform
Stephen R Thom - One of the best experts on this subject based on the ideXlab platform.
-
ascorbic acid supplementation diminishes microparticle elevations and Neutrophil Activation following scuba diving
American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2015Co-Authors: Ming Yang, Otto F Barak, Dennis Madden, Željko Dujić, Jasjeet Bhullar, Veena M. Bhopale, Stephen R ThomAbstract:Predicated on evidence that diving-related microparticle generation is an oxidative stress response, this study investigated the role that oxygen plays in augmenting production of annexin V-positive microparticles associated with open-water SCUBA diving and whether elevations can be abrogated by ascorbic acid. Following a cross-over study design, 14 male subjects ingested placebo and 2–3 wk later ascorbic acid (2 g) daily for 6 days prior to performing either a 47-min dive to 18 m of sea water while breathing air (∼222 kPa N2/59 kPa O2) or breathing a mixture of 60% O2/balance N2 from a tight-fitting face mask at atmospheric pressure for 47 min (∼40 kPa N2/59 kPa O2). Within 30 min after the 18-m dive in the placebo group, Neutrophil Activation, and platelet-Neutrophil interactions occurred, and the total number of microparticles, as well as subgroups bearing CD66b, CD41, CD31, CD142 proteins or nitrotyrosine, increased approximately twofold. No significant elevations occurred among divers after ingesting...
-
exercise before scuba diving ameliorates decompression induced Neutrophil Activation
Medicine and Science in Sports and Exercise, 2014Co-Authors: Dennis Madden, Veena M. Bhopale, Stephen R Thom, Ming Yang, Tatyana N Milovanova, Marko Ljubkovic, Zeljko DujicAbstract:AB Introduction: The goals of this study were to investigate the difference in responses between a scuba dive preceded by aerobic exercise (EX) and a nonexercise control dive (CON) and to further evaluate the potential relation between venous gas emboli (VGE) and microparticles (MP). We hypothesized that exercise would alter the quantity and subtype of annexin V-positive MP and VGE. Methods: Nineteen divers performed two dives to 18 m seawater for 41 min separated by at least 3 d, one of which was preceded by 60 min of treadmill interval exercise. Blood was obtained before exercise, before diving, and 15 min, 2 h, 4 h, and 24 h after surfacing. Intravascular bubbles were quantified by transthoracic echocardiography at 15, 40, 80, and 120 min. Results: The median VGE remained unchanged between the two dives; however, there was a significant increase in VGE in the exercise dive at 40 and 80 min at rest. MP were significantly elevated by approximately 2 times at all time points after CON compared with those after EX. Markers of Neutrophil and platelet Activation were elevated by both dives, and these elevations were attenuated in the EX dive. Conclusions: We conclude that some of the differences observed between the EX and CON related to MP and platelet and Neutrophil Activation provide additional insight into the potential protective benefits of exercise; however, further study is needed to understand the mechanism and true potential of these benefits.
-
bubbles microparticles and Neutrophil Activation changes with exercise level and breathing gas during open water scuba diving
Journal of Applied Physiology, 2013Co-Authors: Stephen R Thom, Dennis Madden, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Mislav LozoAbstract:The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V-positive microparticle (MP) production and size changes and Neutrophil Activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20-min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil Activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-Neutrophil interactions, and Neutrophil Activation appear to exist, but more study is required to improve confidence in the associations.
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that chan...
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, Neutrophil Activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the Neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant invers...
Zeljko Dujic - One of the best experts on this subject based on the ideXlab platform.
-
exercise before scuba diving ameliorates decompression induced Neutrophil Activation
Medicine and Science in Sports and Exercise, 2014Co-Authors: Dennis Madden, Veena M. Bhopale, Stephen R Thom, Ming Yang, Tatyana N Milovanova, Marko Ljubkovic, Zeljko DujicAbstract:AB Introduction: The goals of this study were to investigate the difference in responses between a scuba dive preceded by aerobic exercise (EX) and a nonexercise control dive (CON) and to further evaluate the potential relation between venous gas emboli (VGE) and microparticles (MP). We hypothesized that exercise would alter the quantity and subtype of annexin V-positive MP and VGE. Methods: Nineteen divers performed two dives to 18 m seawater for 41 min separated by at least 3 d, one of which was preceded by 60 min of treadmill interval exercise. Blood was obtained before exercise, before diving, and 15 min, 2 h, 4 h, and 24 h after surfacing. Intravascular bubbles were quantified by transthoracic echocardiography at 15, 40, 80, and 120 min. Results: The median VGE remained unchanged between the two dives; however, there was a significant increase in VGE in the exercise dive at 40 and 80 min at rest. MP were significantly elevated by approximately 2 times at all time points after CON compared with those after EX. Markers of Neutrophil and platelet Activation were elevated by both dives, and these elevations were attenuated in the EX dive. Conclusions: We conclude that some of the differences observed between the EX and CON related to MP and platelet and Neutrophil Activation provide additional insight into the potential protective benefits of exercise; however, further study is needed to understand the mechanism and true potential of these benefits.
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that chan...
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, Neutrophil Activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the Neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant invers...
Veena M. Bhopale - One of the best experts on this subject based on the ideXlab platform.
-
ascorbic acid supplementation diminishes microparticle elevations and Neutrophil Activation following scuba diving
American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2015Co-Authors: Ming Yang, Otto F Barak, Dennis Madden, Željko Dujić, Jasjeet Bhullar, Veena M. Bhopale, Stephen R ThomAbstract:Predicated on evidence that diving-related microparticle generation is an oxidative stress response, this study investigated the role that oxygen plays in augmenting production of annexin V-positive microparticles associated with open-water SCUBA diving and whether elevations can be abrogated by ascorbic acid. Following a cross-over study design, 14 male subjects ingested placebo and 2–3 wk later ascorbic acid (2 g) daily for 6 days prior to performing either a 47-min dive to 18 m of sea water while breathing air (∼222 kPa N2/59 kPa O2) or breathing a mixture of 60% O2/balance N2 from a tight-fitting face mask at atmospheric pressure for 47 min (∼40 kPa N2/59 kPa O2). Within 30 min after the 18-m dive in the placebo group, Neutrophil Activation, and platelet-Neutrophil interactions occurred, and the total number of microparticles, as well as subgroups bearing CD66b, CD41, CD31, CD142 proteins or nitrotyrosine, increased approximately twofold. No significant elevations occurred among divers after ingesting...
-
exercise before scuba diving ameliorates decompression induced Neutrophil Activation
Medicine and Science in Sports and Exercise, 2014Co-Authors: Dennis Madden, Veena M. Bhopale, Stephen R Thom, Ming Yang, Tatyana N Milovanova, Marko Ljubkovic, Zeljko DujicAbstract:AB Introduction: The goals of this study were to investigate the difference in responses between a scuba dive preceded by aerobic exercise (EX) and a nonexercise control dive (CON) and to further evaluate the potential relation between venous gas emboli (VGE) and microparticles (MP). We hypothesized that exercise would alter the quantity and subtype of annexin V-positive MP and VGE. Methods: Nineteen divers performed two dives to 18 m seawater for 41 min separated by at least 3 d, one of which was preceded by 60 min of treadmill interval exercise. Blood was obtained before exercise, before diving, and 15 min, 2 h, 4 h, and 24 h after surfacing. Intravascular bubbles were quantified by transthoracic echocardiography at 15, 40, 80, and 120 min. Results: The median VGE remained unchanged between the two dives; however, there was a significant increase in VGE in the exercise dive at 40 and 80 min at rest. MP were significantly elevated by approximately 2 times at all time points after CON compared with those after EX. Markers of Neutrophil and platelet Activation were elevated by both dives, and these elevations were attenuated in the EX dive. Conclusions: We conclude that some of the differences observed between the EX and CON related to MP and platelet and Neutrophil Activation provide additional insight into the potential protective benefits of exercise; however, further study is needed to understand the mechanism and true potential of these benefits.
-
bubbles microparticles and Neutrophil Activation changes with exercise level and breathing gas during open water scuba diving
Journal of Applied Physiology, 2013Co-Authors: Stephen R Thom, Dennis Madden, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Mislav LozoAbstract:The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V-positive microparticle (MP) production and size changes and Neutrophil Activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20-min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil Activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-Neutrophil interactions, and Neutrophil Activation appear to exist, but more study is required to improve confidence in the associations.
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that chan...
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, Neutrophil Activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the Neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant invers...
Ming Yang - One of the best experts on this subject based on the ideXlab platform.
-
ascorbic acid supplementation diminishes microparticle elevations and Neutrophil Activation following scuba diving
American Journal of Physiology-regulatory Integrative and Comparative Physiology, 2015Co-Authors: Ming Yang, Otto F Barak, Dennis Madden, Željko Dujić, Jasjeet Bhullar, Veena M. Bhopale, Stephen R ThomAbstract:Predicated on evidence that diving-related microparticle generation is an oxidative stress response, this study investigated the role that oxygen plays in augmenting production of annexin V-positive microparticles associated with open-water SCUBA diving and whether elevations can be abrogated by ascorbic acid. Following a cross-over study design, 14 male subjects ingested placebo and 2–3 wk later ascorbic acid (2 g) daily for 6 days prior to performing either a 47-min dive to 18 m of sea water while breathing air (∼222 kPa N2/59 kPa O2) or breathing a mixture of 60% O2/balance N2 from a tight-fitting face mask at atmospheric pressure for 47 min (∼40 kPa N2/59 kPa O2). Within 30 min after the 18-m dive in the placebo group, Neutrophil Activation, and platelet-Neutrophil interactions occurred, and the total number of microparticles, as well as subgroups bearing CD66b, CD41, CD31, CD142 proteins or nitrotyrosine, increased approximately twofold. No significant elevations occurred among divers after ingesting...
-
exercise before scuba diving ameliorates decompression induced Neutrophil Activation
Medicine and Science in Sports and Exercise, 2014Co-Authors: Dennis Madden, Veena M. Bhopale, Stephen R Thom, Ming Yang, Tatyana N Milovanova, Marko Ljubkovic, Zeljko DujicAbstract:AB Introduction: The goals of this study were to investigate the difference in responses between a scuba dive preceded by aerobic exercise (EX) and a nonexercise control dive (CON) and to further evaluate the potential relation between venous gas emboli (VGE) and microparticles (MP). We hypothesized that exercise would alter the quantity and subtype of annexin V-positive MP and VGE. Methods: Nineteen divers performed two dives to 18 m seawater for 41 min separated by at least 3 d, one of which was preceded by 60 min of treadmill interval exercise. Blood was obtained before exercise, before diving, and 15 min, 2 h, 4 h, and 24 h after surfacing. Intravascular bubbles were quantified by transthoracic echocardiography at 15, 40, 80, and 120 min. Results: The median VGE remained unchanged between the two dives; however, there was a significant increase in VGE in the exercise dive at 40 and 80 min at rest. MP were significantly elevated by approximately 2 times at all time points after CON compared with those after EX. Markers of Neutrophil and platelet Activation were elevated by both dives, and these elevations were attenuated in the EX dive. Conclusions: We conclude that some of the differences observed between the EX and CON related to MP and platelet and Neutrophil Activation provide additional insight into the potential protective benefits of exercise; however, further study is needed to understand the mechanism and true potential of these benefits.
-
bubbles microparticles and Neutrophil Activation changes with exercise level and breathing gas during open water scuba diving
Journal of Applied Physiology, 2013Co-Authors: Stephen R Thom, Dennis Madden, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Mislav LozoAbstract:The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V-positive microparticle (MP) production and size changes and Neutrophil Activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20-min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil Activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-Neutrophil interactions, and Neutrophil Activation appear to exist, but more study is required to improve confidence in the associations.
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that chan...
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, Neutrophil Activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the Neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant invers...
Marko Ljubkovic - One of the best experts on this subject based on the ideXlab platform.
-
exercise before scuba diving ameliorates decompression induced Neutrophil Activation
Medicine and Science in Sports and Exercise, 2014Co-Authors: Dennis Madden, Veena M. Bhopale, Stephen R Thom, Ming Yang, Tatyana N Milovanova, Marko Ljubkovic, Zeljko DujicAbstract:AB Introduction: The goals of this study were to investigate the difference in responses between a scuba dive preceded by aerobic exercise (EX) and a nonexercise control dive (CON) and to further evaluate the potential relation between venous gas emboli (VGE) and microparticles (MP). We hypothesized that exercise would alter the quantity and subtype of annexin V-positive MP and VGE. Methods: Nineteen divers performed two dives to 18 m seawater for 41 min separated by at least 3 d, one of which was preceded by 60 min of treadmill interval exercise. Blood was obtained before exercise, before diving, and 15 min, 2 h, 4 h, and 24 h after surfacing. Intravascular bubbles were quantified by transthoracic echocardiography at 15, 40, 80, and 120 min. Results: The median VGE remained unchanged between the two dives; however, there was a significant increase in VGE in the exercise dive at 40 and 80 min at rest. MP were significantly elevated by approximately 2 times at all time points after CON compared with those after EX. Markers of Neutrophil and platelet Activation were elevated by both dives, and these elevations were attenuated in the EX dive. Conclusions: We conclude that some of the differences observed between the EX and CON related to MP and platelet and Neutrophil Activation provide additional insight into the potential protective benefits of exercise; however, further study is needed to understand the mechanism and true potential of these benefits.
-
bubbles microparticles and Neutrophil Activation changes with exercise level and breathing gas during open water scuba diving
Journal of Applied Physiology, 2013Co-Authors: Stephen R Thom, Dennis Madden, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Mislav LozoAbstract:The study goal was to evaluate responses in humans following decompression from open-water SCUBA diving with the hypothesis that exertion underwater and use of a breathing mixture containing more oxygen and less nitrogen (enriched air nitrox) would alter annexin V-positive microparticle (MP) production and size changes and Neutrophil Activation, as well as their relationships to intravascular bubble formation. Twenty-four divers followed a uniform dive profile to 18 m of sea water breathing air or 22.5 m breathing 32% oxygen/68% nitrogen for 47 min, either swimming with moderately heavy exertion underwater or remaining stationary at depth. Blood was obtained pre- and at 15 and 120 min postdive. Intravascular bubbles were quantified by transthoracic echocardiography postdive at 20-min intervals for 2 h. There were no significant differences in maximum bubble scores among the dives. MP number increased 2.7-fold, on average, within 15 min after each dive; only the air-exertion dive resulted in a significant further increase to 5-fold over baseline at 2 h postdive. Neutrophil Activation occurred after all dives. For the enriched air nitrox stationary at depth dive, but not for other conditions, the numbers of postdive annexin V-positive particles above 1 μm in diameter were correlated with intravascular bubble scores (correlation coefficients ∼0.9, P < 0.05). We conclude that postdecompression relationships among bubbles, MPs, platelet-Neutrophil interactions, and Neutrophil Activation appear to exist, but more study is required to improve confidence in the associations.
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that chan...
-
microparticle production Neutrophil Activation and intravascular bubbles following open water scuba diving
Journal of Applied Physiology, 2012Co-Authors: Stephen R Thom, Veena M. Bhopale, Ming Yang, Tatyana N Milovanova, Marina Bogush, Kim Bushmann, Neal W Pollock, Marko Ljubkovic, Petar J Denoble, Zeljko DujicAbstract:The goal of this study was to evaluate annexin V-positive microparticles (MPs) and Neutrophil Activation in humans following decompression from open-water SCUBA diving with the hypothesis that changes are related to intravascular bubble formation. Sixteen male volunteer divers followed a uniform profile of four daily SCUBA dives to 18 m of sea water for 47 min. Blood was obtained prior to and at 80 min following the first and fourth dives to evaluate the impact of repetitive diving, and intravascular bubbles were quantified by trans-thoracic echocardiography carried out at 20-min intervals for 2 h after each dive. MPs increased by 3.4-fold after each dive, Neutrophil Activation occurred as assessed by surface expression of myeloperoxidase and the CD18 component of β2-integrins, and there was an increased presence of the platelet-derived CD41 protein on the Neutrophil surface indicating interactions with platelet membranes. Intravascular bubbles were detected in all divers. Surprisingly, significant invers...