The Experts below are selected from a list of 306 Experts worldwide ranked by ideXlab platform

Nobuhiro Uyesaka - One of the best experts on this subject based on the ideXlab platform.

  • impaired erythrocyte filterability of spontaneously hypertensive rats investigation by Nickel Mesh filtration technique
    Circulation, 2010
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter - ability in SHR is considered to contribute to the development and maintenance of genetic hypertension. (Circ J 2010; 74: 129 - 136)

  • Impaired erythrocyte filterability of spontaneously hypertensive rats : Investigation by Nickel Mesh filtration technique
    Circulation, 2009
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P

  • Causal role of dense microspherocytes in the anemia of hereditary spherocytosis: Clinical importance of filterability measurements through 3-μm pores
    Clinical Hemorheology and Microcirculation, 1994
    Co-Authors: S. Hasegawa, Hideo Shio, M. Iino, Alan N Schechter, T. Nomura, Nobuhiro Uyesaka
    Abstract:

    through the 3-llm pore Nickel Mesh compared with that through the 5-llm pores. It is, therefore, likely that the impaired deformability of HS RBCs arises from the decrease in the cell surface area to volume ratio and the high mean corpuscular hemoglobin concentration. After splenectomy, the dense microspherocytes disappeared, and there was a marked improvement in RBC filterability through 3-llm pores. This suggests that dense cells were microspherocytes that received splenic conditioning in the walls of splenic sinuses. Correspondingly, splenectomy markedly improved clinical manifestations and ameliorated hemolysis. Thus, the laboratory measurement (i.e., the 3-llm filtration test) reflected the patient's clinical state. The use of

  • Effect of low-osmolality contrast media on red cell filterability.
    Acta Radiologica, 1993
    Co-Authors: Hiroyuki Hiruma, Tatsuo Kumazaki, Nobuhiro Uyesaka
    Abstract:

    The effects of low-osmolality contrast media (CM) on red blood cell (RBC) filterability were investigated using a recently developed Nickel Mesh filtration method. the conventional hypertonic CM iothalamate, low-osmolality iohexol, and the recently synthesized iomeprol were studied. Among them, the osmolality of iomeprol was the lowest. the impact of CM osmolality, viscosity, and iodine content on the RBC filterability was analyzed. Under equal iodine content or viscosity condition, the filterability order of RBCs suspended in CM was iomeprol> iohexol >> iothalamate, because of the osmolality of CM. Iomeprol caused small echinocytic changes but these had a negligible influence on RBC filterability. In conclusion, the osmotic effect of CM on RBC filterability is more predominant than the other CM effects, and iomeprol is the preferred CM for RBC filterability.

Koichi Akashi - One of the best experts on this subject based on the ideXlab platform.

  • Impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients: investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Introduction Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Methods Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients ( n  = 101) and age-matched normotensive subjects ( n  = 14) were obtained from medical records, and diabetic patients were excluded. Results Erythrocyte deformability in the hypertensive group was significantly ( p  = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional ( r  = −0.303, p  = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP ( p  = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. Discussion These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients (n = 101) and age-matched normotensive subjects (n = 14) were obtained from medical records, and diabetic patients were excluded. Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional (r = −0.303, p = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP (p = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • Impaired deformability of erythrocytes in diabetic rat and human: investigation by the Nickel-Mesh-filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n  = 8, compared with 83.1 ± 4.2%, n  = 8; p  

  • impaired deformability of erythrocytes in diabetic rat and human investigation by the Nickel Mesh filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n = 8, compared with 83.1 ± 4.2%, n = 8; p < 0.001). Likewise, erythrocyte filterability for diabetic patients was significantly impaired compared with that for controls (87.6 ± 3.4%, n = 174, compared with 88.6 ± 2.1%, n = 51; p = 0.046). Stepwise multiple regression analysis revealed that this impairment was mostly attributable to associated obesity (BMI, p = 0.029) and glycemic stress (HbA1c(JDS), p = 0.046). We therefore conclude that erythrocyte filterability is commonly impaired in diabetic rats and in humans. Moreover, metabolic risk accumulation further impairs erythrocyte filterability, resulting in derangement of the microcirculation.

  • impaired erythrocyte filterability of spontaneously hypertensive rats investigation by Nickel Mesh filtration technique
    Circulation, 2010
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter - ability in SHR is considered to contribute to the development and maintenance of genetic hypertension. (Circ J 2010; 74: 129 - 136)

Keita Odashiro - One of the best experts on this subject based on the ideXlab platform.

  • Impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients: investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Introduction Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Methods Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients ( n  = 101) and age-matched normotensive subjects ( n  = 14) were obtained from medical records, and diabetic patients were excluded. Results Erythrocyte deformability in the hypertensive group was significantly ( p  = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional ( r  = −0.303, p  = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP ( p  = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. Discussion These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients (n = 101) and age-matched normotensive subjects (n = 14) were obtained from medical records, and diabetic patients were excluded. Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional (r = −0.303, p = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP (p = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • Impaired deformability of erythrocytes in diabetic rat and human: investigation by the Nickel-Mesh-filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n  = 8, compared with 83.1 ± 4.2%, n  = 8; p  

  • impaired deformability of erythrocytes in diabetic rat and human investigation by the Nickel Mesh filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n = 8, compared with 83.1 ± 4.2%, n = 8; p < 0.001). Likewise, erythrocyte filterability for diabetic patients was significantly impaired compared with that for controls (87.6 ± 3.4%, n = 174, compared with 88.6 ± 2.1%, n = 51; p = 0.046). Stepwise multiple regression analysis revealed that this impairment was mostly attributable to associated obesity (BMI, p = 0.029) and glycemic stress (HbA1c(JDS), p = 0.046). We therefore conclude that erythrocyte filterability is commonly impaired in diabetic rats and in humans. Moreover, metabolic risk accumulation further impairs erythrocyte filterability, resulting in derangement of the microcirculation.

  • impaired erythrocyte filterability of spontaneously hypertensive rats investigation by Nickel Mesh filtration technique
    Circulation, 2010
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter - ability in SHR is considered to contribute to the development and maintenance of genetic hypertension. (Circ J 2010; 74: 129 - 136)

Toru Maruyama - One of the best experts on this subject based on the ideXlab platform.

  • Impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients: investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Introduction Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Methods Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients ( n  = 101) and age-matched normotensive subjects ( n  = 14) were obtained from medical records, and diabetic patients were excluded. Results Erythrocyte deformability in the hypertensive group was significantly ( p  = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional ( r  = −0.303, p  = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP ( p  = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. Discussion These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients (n = 101) and age-matched normotensive subjects (n = 14) were obtained from medical records, and diabetic patients were excluded. Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional (r = −0.303, p = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP (p = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • Impaired deformability of erythrocytes in diabetic rat and human: investigation by the Nickel-Mesh-filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n  = 8, compared with 83.1 ± 4.2%, n  = 8; p  

  • impaired deformability of erythrocytes in diabetic rat and human investigation by the Nickel Mesh filtration technique
    Journal of Biorheology, 2011
    Co-Authors: Y. Kogawa, Keita Odashiro, Koichi Akashi, Toru Maruyama, Masaki Fukata, T. Fujino
    Abstract:

    Comprehensive research to quantify the deformability of erythrocytes in diabetic animals and humans has been lacking. The objective of this study was to compare the impairment of erythrocyte deformability in diabetic rats and patients by use of the same rheologic method. Deformability was investigated in streptozotocin-induced diabetic rats and diabetic patients, by using the highly sensitive and quantitative Nickel-Mesh-filtration technique. Erythrocyte filterability (whole-cell deformability) was defined as flow rate of hematocrit-adjusted erythrocyte suspension relative to that of saline (%). Hematological and biochemical data for diabetic rats did not differ from those for age-matched control rats except for hyperglycemia and malnutrition. Erythrocyte filterability for diabetic rats was significantly lower than that for control rats (69.4 ± 10.1%, n = 8, compared with 83.1 ± 4.2%, n = 8; p < 0.001). Likewise, erythrocyte filterability for diabetic patients was significantly impaired compared with that for controls (87.6 ± 3.4%, n = 174, compared with 88.6 ± 2.1%, n = 51; p = 0.046). Stepwise multiple regression analysis revealed that this impairment was mostly attributable to associated obesity (BMI, p = 0.029) and glycemic stress (HbA1c(JDS), p = 0.046). We therefore conclude that erythrocyte filterability is commonly impaired in diabetic rats and in humans. Moreover, metabolic risk accumulation further impairs erythrocyte filterability, resulting in derangement of the microcirculation.

  • impaired erythrocyte filterability of spontaneously hypertensive rats investigation by Nickel Mesh filtration technique
    Circulation, 2010
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter - ability in SHR is considered to contribute to the development and maintenance of genetic hypertension. (Circ J 2010; 74: 129 - 136)

Takehiko Fujino - One of the best experts on this subject based on the ideXlab platform.

  • Impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients: investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Introduction Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Methods Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients ( n  = 101) and age-matched normotensive subjects ( n  = 14) were obtained from medical records, and diabetic patients were excluded. Results Erythrocyte deformability in the hypertensive group was significantly ( p  = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional ( r  = −0.303, p  = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP ( p  = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. Discussion These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • impaired deformability of circulating erythrocytes obtained from nondiabetic hypertensive patients investigation by a Nickel Mesh filtration technique
    Clinical Hypertension, 2015
    Co-Authors: Keita Odashiro, Takeshi Arita, Takehiko Fujino, Toru Maruyama, Koichi Akashi
    Abstract:

    Hypertension is associated with microcirculatory disturbance, and erythrocyte deformability is a major determinant of the microcirculation. However, impairment of erythrocyte deformability in hypertensive patients in relation to antihypertensive treatment is unclear. The present study aimed to investigate this impairment in hypertensive patients under treatment using a highly sensitive and quantitative Nickel Mesh filtration technique. Deformability was evaluated by filterability, defined as the flow rate of a hematocrit-adjusted erythrocyte suspension relative to that of saline under a specific filtration pressure in a pressure-flow curve obtained by continuous filtration. Baseline characteristics of hypertensive patients (n = 101) and age-matched normotensive subjects (n = 14) were obtained from medical records, and diabetic patients were excluded. Erythrocyte deformability in the hypertensive group was significantly (p = 0.010) lower (87.8 ± 2.2 %) than that of the normotensive group (89.4 ± 1.7 %) and inversely proportional (r = −0.303, p = 0.002) to the mean blood pressure (BP) measured on blood sampling for the filtration study. Stepwise multiple regression analysis demonstrated that this impairment was mostly attributable to the mean BP (p = 0.001), whereas current smoking and episodes of stroke or coronary artery disease were not contributors. These findings indicate that erythrocyte deformability is impaired in the hypertensive patients, which depends on the current BP control rather than target organ damage.

  • impaired erythrocyte filterability of spontaneously hypertensive rats investigation by Nickel Mesh filtration technique
    Circulation, 2010
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P<0.005, and 71.1±3.9% in 18 weeks of age, P<0.005, respectively). This impairment in SHR could not be explained by the mean corpuscular volume or mean corpuscular hemoglobin concentration of erythrocytes, but the erythrocyte count was significantly (P<0.005) greater in SHR than in the age-matched WKY. Conclusions: Although the precise mechanisms remain to be elucidated, markedly impaired erythrocyte filter - ability in SHR is considered to contribute to the development and maintenance of genetic hypertension. (Circ J 2010; 74: 129 - 136)

  • Impaired erythrocyte filterability of spontaneously hypertensive rats : Investigation by Nickel Mesh filtration technique
    Circulation, 2009
    Co-Authors: Kyoko Ariyoshi, Keita Odashiro, Koichi Akashi, Takehiko Fujino, Toru Maruyama, Nobuhiro Uyesaka
    Abstract:

    Background: Deformability of erythrocytes plays a key role in the impairment of the microcirculation in hyper- tension. However, erythrocyte deformability in spontaneously hypertensive rats (SHR) during development of hypertension has not been fully investigated so far. Methods and Results: Erythrocyte filterability (whole cell deformability) was investigated in relation to blood pressure measured by the tail-cuff method in SHR and age-matched Wistar-Kyoto rats (WKY), using a highly sensitive and reproducible Nickel Mesh filtration technique. Impaired erythrocyte filterability was marked (37.0±17.5%) in prehypertensive young SHR (7 weeks of age) and sustained (51.6±13.3%) in hypertensive mature SHR (18 weeks of age), when compared with that of age-matched WKY (62.1±7.2% in 7 weeks of age, P