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Franz Bucar - One of the best experts on this subject based on the ideXlab platform.

  • Antioxidant activity of Nigella sativa essential oil
    Phytotherapy Research, 2000
    Co-Authors: M. Burits, Franz Bucar
    Abstract:

    The essential oil of black cumin seeds, Nigella sativa L., was tested for a possible antioxidant activity. A rapid evaluation for antioxidants, using two TLC screening methods, showed that thymoquinone and the components carvacrol, t-anethole and 4-terpineol demonstrated respectable radical scavenging property. These four constituents and the essential oil possessed variable antioxidant activity when tested in the diphenylpicrylhydracyl assay for non-specific hydrogen atom or electron donating activity. They were also effective.OH radical scavenging agents in the assay for non-enzymatic lipid peroxidation in liposomes and the deoxyribose degradation assay. GC-MS analysis of the essential oil obtained from six different samples of Nigella sativa seeds and from a commercial fixed oil showed that the qualitative composition of the volatile compounds was almost identical. Differences were mainly restricted to the quantitative composition.

Ali Aldossary - One of the best experts on this subject based on the ideXlab platform.

  • antidermatophyte activity of ether extract of Nigella sativa and its active principle thymoquinone
    Journal of Ethnopharmacology, 2005
    Co-Authors: Salih H M Aljabre, Mohamad Akram Randhawa, Naeem Akhtar, Omar M Alakloby, Abdulrahman M Alqurashi, Ali Aldossary
    Abstract:

    Abstract The antifungal activity of ether extract of Nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of Trichophyton rubrum and one each of Trichophyton interdigitale , Trichophyton mentagrophytes , Epidermophyton floccosum and Microsporum canis . Agar diffusion method with serial dilutions of ether extract of Nigella sativa , thymoquinone and griseofulvin was employed. The incubation was carried out at 30 °C for 14 days. The diameter of fungal colonies and the percentage inhibition of the fungal growth at each dilution were determined, taking those of the controls as 100%. The minimum inhibitory concentration (MIC) was considered as the minimum concentration of the drug, which inhibited 80–100% of the fungal growth. The MICs of the ether extract of Nigella sativa and thymoquinone were between 10 and 40 and 0.125 and 0.25 mg/ml, respectively, while those of griseofulvin ranged from 0.00095 to 0.0155 mg/ml. These results denote the potentiality of Nigella sativa as a source for antidermatophyte drugs and support its use in folk medicine for the treatment of fungal skin infections.

  • Antidermatophyte activity of ether extract of Nigella sativa and its active principle, thymoquinone.
    Journal of ethnopharmacology, 2005
    Co-Authors: Salih H M Aljabre, Mohamad Akram Randhawa, Naeem Akhtar, Omar M Alakloby, Abdulrahman M Alqurashi, Ali Aldossary
    Abstract:

    The antifungal activity of ether extract of Nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of Trichophyton rubrum and one each of Trichophyton interdigitale, Trichophyton mentagrophytes, Epidermophyton floccosum and Microsporum canis. Agar diffusion method with serial dilutions of ether extract of Nigella sativa, thymoquinone and griseofulvin was employed. The incubation was carried out at 30 degrees C for 14 days. The diameter of fungal colonies and the percentage inhibition of the fungal growth at each dilution were determined, taking those of the controls as 100%. The minimum inhibitory concentration (MIC) was considered as the minimum concentration of the drug, which inhibited 80-100% of the fungal growth. The MICs of the ether extract of Nigella sativa and thymoquinone were between 10 and 40 and 0.125 and 0.25 mg/ml, respectively, while those of griseofulvin ranged from 0.00095 to 0.0155 mg/ml. These results denote the potentiality of Nigella sativa as a source for antidermatophyte drugs and support its use in folk medicine for the treatment of fungal skin infections.

M. Hassar - One of the best experts on this subject based on the ideXlab platform.

  • acute and chronic toxicity of Nigella sativa fixed oil
    Phytomedicine, 2002
    Co-Authors: A. Zaoui, Yahia Cherrah, H. Amarouch, N Mahassini, Katim Alaoui, M. Hassar
    Abstract:

    Summary We investigated the toxicity of the fixed oil of Nigella sativa L seeds in mice and rats through determination of LD 50 values and examination of possible biochemical, hematological and histopathological changes. The acute toxicity of Nigella sativa fixed oil was investigated in mice. LD 50 values, obtained by single doses, orally and intraperitoneally administered in mice, were 28.8 ml/kg body wt. p.o. [26.2–31.6] and 2.06 ml/kg body wt. i.p. [1.86–2.26], respectively. Chronic toxicity was studied in rats treated daily with an oral dose of 2 ml/kg body wt. for 12 weeks. Changes in key hepatic enzymes levels, including aspartate-aminotransferase, alanine-aminotranferase, and gamma-glutamyltransferase and histopathological modifications (heart, liver, kidneys and pancreas) were not observed in rats treated with Nigella sativa after 12 weeks of treatment. The serum cholesterol, triglyceride and glucose levels and the count of leukocytes and platelets decreased significantly, compared to control values, while hematocrit and hemoglobin levels increased significantly. A slowing of body weight gain was also observed in Nigella sativa treated rats, as compared to control animals. The low toxicity of Nigella sativa fixed oil, evidenced by high LD 50 values, key hepatic enzyme stability and organ integrity, suggests a wide margin of safety for therapeutic doses of Nigella sativa fixed oil, but the changes in hemoglobin metabolism and the fall in leukocyte and platelet count must be taken into consideration.

  • Diuretic and hypotensive effects of Nigella sativa in the spontaneously hypertensive rat
    Therapie, 2000
    Co-Authors: A. Zaoui, A Settaf, Yahia Cherrah, M. A. Lacaille-dubois, H. Amarouch, M. Hassar
    Abstract:

    Nigella sativa (ranunculaceae) is used in Arab folk medicine as a diuretic and hypotensive plant. We report here the diuretic and hypotensive effects of dichloromethane extract of Nigella sativa seeds in the spontaneously hypertensive rat (SHR). An oral dose of Nigella sativa extract (0.6 ml/kg/day) and furosemide (5 mg/kg/day) increased significantly the diuresis by 16 and 30 per cent respectively after 15 days of treatment; urinary excretion of Cl-, Na+, K+ and urea is also increased. Simultaneously, the mean arterial pressure decreased respectively by 22 and 18 per cent in the Nigella sativa treated rat and nifedipine treated rat (0.5 mg/kg/day). In conclusion, the diuretic activity observed in the SHR rat treated with Nigella sativa seeds may be partially responsible for its diuretic action; it seems that other pathways may also be involved in their cardiovascular effects.

M. Burits - One of the best experts on this subject based on the ideXlab platform.

  • Antioxidant activity of Nigella sativa essential oil
    Phytotherapy Research, 2000
    Co-Authors: M. Burits, Franz Bucar
    Abstract:

    The essential oil of black cumin seeds, Nigella sativa L., was tested for a possible antioxidant activity. A rapid evaluation for antioxidants, using two TLC screening methods, showed that thymoquinone and the components carvacrol, t-anethole and 4-terpineol demonstrated respectable radical scavenging property. These four constituents and the essential oil possessed variable antioxidant activity when tested in the diphenylpicrylhydracyl assay for non-specific hydrogen atom or electron donating activity. They were also effective.OH radical scavenging agents in the assay for non-enzymatic lipid peroxidation in liposomes and the deoxyribose degradation assay. GC-MS analysis of the essential oil obtained from six different samples of Nigella sativa seeds and from a commercial fixed oil showed that the qualitative composition of the volatile compounds was almost identical. Differences were mainly restricted to the quantitative composition.

Salih H M Aljabre - One of the best experts on this subject based on the ideXlab platform.

  • antidermatophyte activity of ether extract of Nigella sativa and its active principle thymoquinone
    Journal of Ethnopharmacology, 2005
    Co-Authors: Salih H M Aljabre, Mohamad Akram Randhawa, Naeem Akhtar, Omar M Alakloby, Abdulrahman M Alqurashi, Ali Aldossary
    Abstract:

    Abstract The antifungal activity of ether extract of Nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of Trichophyton rubrum and one each of Trichophyton interdigitale , Trichophyton mentagrophytes , Epidermophyton floccosum and Microsporum canis . Agar diffusion method with serial dilutions of ether extract of Nigella sativa , thymoquinone and griseofulvin was employed. The incubation was carried out at 30 °C for 14 days. The diameter of fungal colonies and the percentage inhibition of the fungal growth at each dilution were determined, taking those of the controls as 100%. The minimum inhibitory concentration (MIC) was considered as the minimum concentration of the drug, which inhibited 80–100% of the fungal growth. The MICs of the ether extract of Nigella sativa and thymoquinone were between 10 and 40 and 0.125 and 0.25 mg/ml, respectively, while those of griseofulvin ranged from 0.00095 to 0.0155 mg/ml. These results denote the potentiality of Nigella sativa as a source for antidermatophyte drugs and support its use in folk medicine for the treatment of fungal skin infections.

  • Antidermatophyte activity of ether extract of Nigella sativa and its active principle, thymoquinone.
    Journal of ethnopharmacology, 2005
    Co-Authors: Salih H M Aljabre, Mohamad Akram Randhawa, Naeem Akhtar, Omar M Alakloby, Abdulrahman M Alqurashi, Ali Aldossary
    Abstract:

    The antifungal activity of ether extract of Nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of Trichophyton rubrum and one each of Trichophyton interdigitale, Trichophyton mentagrophytes, Epidermophyton floccosum and Microsporum canis. Agar diffusion method with serial dilutions of ether extract of Nigella sativa, thymoquinone and griseofulvin was employed. The incubation was carried out at 30 degrees C for 14 days. The diameter of fungal colonies and the percentage inhibition of the fungal growth at each dilution were determined, taking those of the controls as 100%. The minimum inhibitory concentration (MIC) was considered as the minimum concentration of the drug, which inhibited 80-100% of the fungal growth. The MICs of the ether extract of Nigella sativa and thymoquinone were between 10 and 40 and 0.125 and 0.25 mg/ml, respectively, while those of griseofulvin ranged from 0.00095 to 0.0155 mg/ml. These results denote the potentiality of Nigella sativa as a source for antidermatophyte drugs and support its use in folk medicine for the treatment of fungal skin infections.