The Experts below are selected from a list of 114 Experts worldwide ranked by ideXlab platform
Susan Love - One of the best experts on this subject based on the ideXlab platform.
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Cancer: Proceedings Summary of The Sixth International Symposium on the Intraductal Approach To Breast Cancer Santa
2016Co-Authors: Susan Rochman, Dixie Mills, Henry Kuerer, Susan Love, Julian KimAbstract:Researchers are using the intraductal approach to advance breast cancer risk assessment, prevention, diagnosis, and treatment. Procedures and technologies that can access and interrogate the ductal-alveolar systems include Nipple Aspiration, ductal lavage and ductoscopy. Ductoscopic papillectomy, ductoscopic margin evaluation, and intraductal therapy are considered promising investigational and innovative treatments. These techniques are used to explore the biology of the normal breast; collect and analyze breast fluid and cells to identify biomarkers that can be used in breast cancer detection and risk assessment; and to identify new ways to find and administer therapeutic and/or preventive agents to the breast tissue. This report summarizes the latest research findings in these areas, presented at The 6th International Symposium on the Intraductal Approach to Breast Cancer in 2009
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State of the Science and the Intraductal Approach for Breast Cancer: Proceedings Summary of The Sixth International Symposium on the Intraductal Approach To Breast Cancer Santa Monica, California, 19–21 February 2009
BMC Proceedings, 2009Co-Authors: Susan Rochman, Dixie Mills, Henry Kuerer, Susan LoveAbstract:Researchers are using the intraductal approach to advance breast cancer risk assessment, prevention, diagnosis, and treatment. Procedures and technologies that can access and interrogate the ductal-alveolar systems include Nipple Aspiration, ductal lavage and ductoscopy. Ductoscopic papillectomy, ductoscopic margin evaluation, and intraductal therapy are considered promising investigational and innovative treatments. These techniques are used to explore the biology of the normal breast; collect and analyze breast fluid and cells to identify biomarkers that can be used in breast cancer detection and risk assessment; and to identify new ways to find and administer therapeutic and/or preventive agents to the breast tissue. This report summarizes the latest research findings in these areas, presented at The 6^th International Symposium on the Intraductal Approach to Breast Cancer in 2009.
Seema A Khan - One of the best experts on this subject based on the ideXlab platform.
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genetic determinants of Nipple Aspiration fluid yield
Annals of Surgical Oncology, 2016Co-Authors: Ali Shidfar, Jun Wang, Elliot Wiesenfeld, Wei Zhang, Denise M Scholtens, Angela J Fought, Robert T Chatterton, Seema A KhanAbstract:Nipple Aspiration fluid (NAF) is a non-invasively-acquired biosample that can provide a window into the breast environment, but NAF yield is highly variable. Its determinants must be better understood for studies of breast cancer risk. The wet earwax phenotype was identified as one determinant of NAF yield in the 1970s, and is linked to single nucleotide polymorphisms (SNPs) in the ATP-binding cassette transporter gene ABCC11. We have investigated this, as well as SNPs in the prolactin (PRL) and prolactin receptor (PRLR) genes, in relation to NAF yield. DNA was extracted from white blood cells of 557 NAF yielders and 359 non-yielders, and was used to genotype ABCC11 (rs17822931), PRL (rs849870, rs849872, rs849886, rs2244502, rs1341239), and PRLR (rs37364, rs34024951, rs1610218, rs9292575, rs7718468) using Taqman genotyping assay. The association between NAF yield and each single SNP was analyzed using logistic regression adjusting for age, race, and menopausal status. ABCC11 rs17822931 showed a negative association with NAF yield [odds ratio (OR) 0.66, 95 % confidence interval (CI) 0.49–0.88; p = 0.004]. The PRL rs849870 and the haplotype combination with other SNPs showed a marginal association with NAF yield. In addition, the years since last birth also showed negative association with NAF yielding (OR 0.98, 95 % CI 0.96–0.99; p = 0.001). The combination of the years since last birth with ABCC11 SNP revealed significant interaction between reproductive factor and genetic factor. Our results confirmed the association between NAF yield and earwax phenotype through ABCC11 genotype. Combined with the recency of last birth, ABCC11 genotype should be considered in the design of studies utilizing NAF as a biosample.
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abstract p3 07 11 Nipple Aspiration fluid yielders and non yielders genetic characteristics
Cancer Research, 2013Co-Authors: Ali Shidfar, Jun Wang, Denise M Scholtens, Robert T Chatterton, Subhashini Allu, Seema A KhanAbstract:Background: Nipple Aspiration fluid (NAF) is a noninvasively acquired biosample that can provide a window of observation into the breast environment, but NAF yield is variable across studies, and is related to a variety of demographic and reproductive factors. We have recently observed a positive correlation between serum prolactin level and NAF yield status. The genetic traits determining NAF yield are unknown, but data from the 1970s linked wet type ear wax to NAF yield, and recent studies point to single nucleotide polymorphisms (SNPs) in the ABCC11 gene as a determinant of earwax type. Further, prolactin and prolactin receptor SNPs are related to serum prolactin level. We have investigated associations between NAF yield and SNPs in prolactin, prolactin receptor and ABCC11 genes in a recently completed case-control study. Method: NAF and/or blood were collected from 916 women. Subjects were defined as yielder if NAF volume was equal or more than 2μL and non-yielder if it was less than 2μL. According to this definition, our subjects categorized as 557 yielders and 359 non-yielders. DNA was extracted from blood using Qiagen kit and 20 ng of DNA was used to amplify and genotype for ABCC11 (rs17822931), PRLR (rs37364), PRL (rs2244502), PRL (rs849872) SNPs using Taqman genotyping assay on an Applied Biosystems 7900HT machine. Results: The median age of Yielders and Non-yielders was 51 and 54, respectively, with the same range of 31-70 for both groups (P<0.0001). There were 209(38%) premenopausal, 257(46%) postmenopausal and 81(15%) perimenopausal of yielders vs. 99(28%) premenopausal, 229(64%) postmenopausal and 26(7%) perimenopausal of non-yielders (P<0.0001). ABCC11 genotype distribution was significantly different between yielders and non-yielders (P = 0.00163). However, genotype distribution was not significantly different for PRLR (rs37364), PRL (rs2244502) and PRL (rs849872). View this table: Conclusion: Our analysis demonstrates a correlation between ABCC11 (rs17822931) SNP and NAF yield status, with the major homozygous CC allele associated with NAF yielder and minor homozygous TT allele associated with non-yielder status. If NAF-based risk biomarkers are to be developed, a better understanding of the genetics of NAF yield is required. Citation Information: Cancer Res 2013;73(24 Suppl): Abstract nr P3-07-11.
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Ductal Lavage for Detection of Cellular Atypia in Women at High Risk for Breast Cancer
2013Co-Authors: William C Dooley, Seema A Khan, Brittmarie Ljung, Umberto Veronesi, Massimiliano Cazzaniga, Richard M Elledge, David T Hung, Joyce A. O’shaughnessy, Henry M. Kuerer, Rogsbert F PhillipsAbstract:Background: Breast cancer originates in breast epithelium and is associated with progressive molecular and morphologic changes.Women with atypical breast ductal epithelial cells have an increased relative risk of breast cancer.In this study, ductal lavage, a new procedure for collecting ductal cells with a microcatheter, was compared with Nipple Aspiration with regard to safety, tolerability, and the ability to detect abnormal breast epithelial cells. Methods: Women at high risk for breast cancer who had nonsuspicious mammograms and clinical breast examinations underwent Nipple Aspiration followed by lavage of fluid-yielding ducts.All statistical tests were two-sided. Results: The 507 women enrolled included 291 (57%) with a history of breast cancer and 199 (39%) with a 5-year Gail risk for breast cancer of 1.7 % or more. Nipple aspirate fluid (NAF) samples wer
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breast cancer characteristics in yielders and nonyielders of Nipple Aspiration fluid
Journal of Clinical Oncology, 2011Co-Authors: V Brutus, C Mcgathey, Thomas E Kmiecik, Kevin P Bethke, Jacqueline Sara Jeruss, Nora M Hansen, Seema A KhanAbstract:71 Background: Nipple aspirate fluid (NAF) is an attractive biosample for the investigation of breast cancer risk factors. The reported NAF yield rate varies from 30% to 90% in various studies, rai...
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identification of biomarkers for breast cancer in Nipple Aspiration and ductal lavage fluid
Clinical Cancer Research, 2005Co-Authors: Jing Zhao, S Krishnamurthy, Seema A Khan, Julie R Lange, Henry Mark Kuerer, Eric Schilling, Saraswati Sukumar, Daniel W ChanAbstract:Purpose: To establish a comprehensive proteomic approach for biomarker discovery and validation in breast fluid. Experimental Design: A total of 95 specimens from three institutions were used including 10 Nipple Aspiration fluid (5 stage I/II cancerous breasts and 5 age-matched healthy controls), 42 ductal lavage fluid from 14 patients with unilateral stage I/II cancer (25 from 9 cancerous breasts and 17 from 7 contralateral breasts), and 42 ductal lavage fluid from 14 high-risk women (multiple ducts repeated lavage). Differentially expressed protein/peptides were discovered by proteomic analysis of training sample, using ProteinChip arrays and surface-enhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry, and validated on independently collected testing samples. After protein identification, ELISA was done to confirm the SELDI findings. Results: We were able to obtain reproducible protein profiles using minimal amount of protein (1 μg) by applying an optimized chip protocol and SELDI. We were able to select cancer-associated biomarkers despite large individual variability by applying both unsupervised and supervised cluster analysis. Furthermore, we were able to train and test candidate biomarkers on independently collected samples and identified one component of a multimarker panel as human neutrophil peptides 1 to 3. Conclusions: Breast fluid is a rich source of breast cancer biomarkers. In combination with high-throughput novel proteomic profiling technology and multicenter study design, markers that are highly specific to breast cancer can be discovered and validated. Our observations also suggest that persistent elevation of human neutrophil peptide in high-risk women may imply early onset of cancer not yet detectable by current detection method. Proof of this hypothesis requires follow-up on a larger study population.
William C Dooley - One of the best experts on this subject based on the ideXlab platform.
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Ductal Lavage for Detection of Cellular Atypia in Women at High Risk for Breast Cancer
2013Co-Authors: William C Dooley, Seema A Khan, Brittmarie Ljung, Umberto Veronesi, Massimiliano Cazzaniga, Richard M Elledge, David T Hung, Joyce A. O’shaughnessy, Henry M. Kuerer, Rogsbert F PhillipsAbstract:Background: Breast cancer originates in breast epithelium and is associated with progressive molecular and morphologic changes.Women with atypical breast ductal epithelial cells have an increased relative risk of breast cancer.In this study, ductal lavage, a new procedure for collecting ductal cells with a microcatheter, was compared with Nipple Aspiration with regard to safety, tolerability, and the ability to detect abnormal breast epithelial cells. Methods: Women at high risk for breast cancer who had nonsuspicious mammograms and clinical breast examinations underwent Nipple Aspiration followed by lavage of fluid-yielding ducts.All statistical tests were two-sided. Results: The 507 women enrolled included 291 (57%) with a history of breast cancer and 199 (39%) with a 5-year Gail risk for breast cancer of 1.7 % or more. Nipple aspirate fluid (NAF) samples wer
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ductal lavage Nipple Aspiration and ductoscopy for breast cancer diagnosis
Current Oncology Reports, 2003Co-Authors: William C DooleyAbstract:The intraductal approach to breast cancer has been invigorated this year by a series of papers exploring ductalbased screening through Nipple Aspiration and lavage and ductal exploration through endoscopy. The merging of these efforts to define the earliest biologic changes in the progression toward breast cancer is opening new fields for both bench-translational and clinical research. These techniques have already begun to show value in defining the presence and extent of proliferative disease in high-risk patients, allowing for more informed therapeutic decision making.
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ductal lavage for detection of cellular atypia in women at high risk for breast cancer
Journal of the National Cancer Institute, 2001Co-Authors: William C Dooley, Seema A Khan, Henry Mark Kuerer, Brittmarie Ljung, Umberto Veronesi, Massimiliano Cazzaniga, Richard M Elledge, Joyce Oshaughnessy, David T Hung, Rogsbert F PhillipsAbstract:Background: Breast cancer originates in breast epithelium and is associated with progressive molecular and morphologic changes. Women with atypical breast ductal epithelial cells have an increased relative risk of breast cancer. In this study, ductal lavage, a new procedure for collecting ductal cells with a microcatheter, was compared with Nipple Aspiration with regard to safety, tolerability, and the ability to detect abnormal breast epithelial cells. Methods: Women at high risk for breast cancer who had nonsuspicious mammograms and clinical breast examinations underwent Nipple Aspiration followed by lavage of fluid-yielding ducts. All statistical tests were two-sided. Results: The 507 women enrolled included 291 (57%) with a history of breast cancer and 199 (39%) with a 5-year Gail risk for breast cancer of 1.7% or more. Nipple aspirate fluid (NAF) samples were evaluated cytologically for 417 women, and ductal lavage samples were evaluated for 383 women. Adequate samples for diagnosis were collected from 111 (27%) and 299 (78%) women, respectively. A median of 13500 epithelial cells per duct (range, 43-492 000 cells) was collected by ductal lavage compared with a median of 120 epithelial cells per breast (range, 10-74300) collected by Nipple Aspiration. For ductal lavage, 92 (24%) subjects had abnormal cells that were mildly (17%) or markedly (6%) atypical or malignant (<1%). For NAF, corresponding percentages were 6%, 3%, and fewer than 1 %. Ductal lavage detected abnormal intraductal breast cells 3.2 times more often than Nipple Aspiration (79 versus 25 breasts; McNemar's test, P<.001). No serious procedure-related adverse events were reported. Conclusions: Large numbers of ductal cells can be collected by ductal lavage to detect atypical cellular changes within the breast. Ductal lavage is a safe and well-tolerated procedure and is a more sensitive method of detecting cellular atypia than Nipple Aspiration.
Susan Rochman - One of the best experts on this subject based on the ideXlab platform.
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Cancer: Proceedings Summary of The Sixth International Symposium on the Intraductal Approach To Breast Cancer Santa
2016Co-Authors: Susan Rochman, Dixie Mills, Henry Kuerer, Susan Love, Julian KimAbstract:Researchers are using the intraductal approach to advance breast cancer risk assessment, prevention, diagnosis, and treatment. Procedures and technologies that can access and interrogate the ductal-alveolar systems include Nipple Aspiration, ductal lavage and ductoscopy. Ductoscopic papillectomy, ductoscopic margin evaluation, and intraductal therapy are considered promising investigational and innovative treatments. These techniques are used to explore the biology of the normal breast; collect and analyze breast fluid and cells to identify biomarkers that can be used in breast cancer detection and risk assessment; and to identify new ways to find and administer therapeutic and/or preventive agents to the breast tissue. This report summarizes the latest research findings in these areas, presented at The 6th International Symposium on the Intraductal Approach to Breast Cancer in 2009
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State of the Science and the Intraductal Approach for Breast Cancer: Proceedings Summary of The Sixth International Symposium on the Intraductal Approach To Breast Cancer Santa Monica, California, 19–21 February 2009
BMC Proceedings, 2009Co-Authors: Susan Rochman, Dixie Mills, Henry Kuerer, Susan LoveAbstract:Researchers are using the intraductal approach to advance breast cancer risk assessment, prevention, diagnosis, and treatment. Procedures and technologies that can access and interrogate the ductal-alveolar systems include Nipple Aspiration, ductal lavage and ductoscopy. Ductoscopic papillectomy, ductoscopic margin evaluation, and intraductal therapy are considered promising investigational and innovative treatments. These techniques are used to explore the biology of the normal breast; collect and analyze breast fluid and cells to identify biomarkers that can be used in breast cancer detection and risk assessment; and to identify new ways to find and administer therapeutic and/or preventive agents to the breast tissue. This report summarizes the latest research findings in these areas, presented at The 6^th International Symposium on the Intraductal Approach to Breast Cancer in 2009.
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Meeting report The Fourth International Symposium on the Intraductal Approach
2005Co-Authors: Bonnie L King, Susan M Love, Susan Rochman, Julian A KimAbstract:Intraductal approaches encompass procedures and technologies that are designed to access and interrogate the ductal–alveolar systems of the human breast, and include Nipple Aspiration, ductal lavage, random periareolar fine needle Aspiration, and ductoscopy. These approaches are being used to collect and analyze fluids and cells to develop methods for breast cancer detection and risk assessment; to introduce imaging technologies to explore the mammary tree for abnormalities; to administer therapeutic and/or preventive agents directly to the breast tissue; and to explore the biology of the normal mammary gland. The latest research findings in these areas, presented at The 4th International Symposium on the Intraductal Approach to Breast Cancer in 2005, are summarized in this report
Gillian Mitchell - One of the best experts on this subject based on the ideXlab platform.
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Nipple Aspiration and ductal lavage in women with a germline brca1 or brca2 mutation
Breast Cancer Research, 2005Co-Authors: Gillian Mitchell, Yoland Antill, William K Murray, Judy Kirk, Elizabeth Salisbury, Geoffrey J Lindeman, Juliana Di Iulio, Alvin Milner, Lisa Devereaux, Kellyanne PhillipsAbstract:The aim of this study was to collect serial samples of Nipple aspirate (NA) and ductal lavage (DL) fluid from women with germline BRCA1/2 mutations in order to create a biorepository for use in identifying biomarkers of breast cancer risk. Between March 2003 and February 2005, 52 women with germline BRCA1 or BRCA2 mutations (median age 43 years, range 27 to 65 years) were scheduled for six-monthly NA, DL and venesection. DL was attempted for all NA fluid-yielding (FY) and any non-FY ducts that could be located at each visit. Twenty-seven (52%) women were postmenopausal, predominantly (19/27) from risk reducing bilateral salpingo-oophorectomy (BSO). FY ducts were identified in 60% of all women, 76% of premenopausal women versus 44% of postmenopausal (P = 0.026). Eighty-five percent of women had successful DL. Success was most likely in women with FY ducts (FY 94% versus non-FY 71% (P = 0.049). DL samples were more likely to be cellular if collected from FY ducts (FY 68% versus non-FY 43%; P = 0.037). Total cell counts were associated with FY status (FY median cell count 30,996, range 0 to >1,000,000 versus non-FY median cell count 0, range 0 to 173,577; P = 0.002). Four women (8%) had ducts with severe atypia with or without additional ducts with mild epithelial atypia; seven others had ducts with mild atypia alone (11/52 (21%) in total). Median total cell count was greater from ducts with atypia (105,870, range 1920 to >1,000,000) than those with no atypia (174, 0 to >1,000,000; P ≤ 0.001). It is feasible to collect serial NA and DL samples from women at high genetic risk of breast cancer, and we are creating a unique, prospective collection of ductal samples that have the potential to be used for discovery of biomarkers of breast cancer risk and evaluate the ongoing effects of risk reducing BSO. DL cellular atypia was not predictive of a current breast cancer and longer follow up is needed to determine whether atypia is an additional marker of future breast cancer risk in this population already at high genetic risk of breast cancer.
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prostate specific antigen in Nipple Aspiration fluid menstrual cycle variability and correlation with serum prostate specific antigen
Tumor Biology, 2002Co-Authors: Gillian Mitchell, Edward R Sauter, Paul E C Sibley, Penelope A M Wilson, Roger Ahern, Rosalind A EelesAbstract:Objectives: To determine the variability of prostate-specific antigen (PSA) in the breast ductal fluid (Nipple Aspiration fluid, NAF) during the menstrual cycles of healthy premenopausal women. Methods: Fifteen female volunteers underwent weekly Nipple Aspiration of ductal fluid from both breasts for the duration of two menstrual cycles. A highly sensitive and specific Third Generation PSA Assay (IMMULITE®; Diagnostic Products Corporation, DPC) was used to detect NAF and serum PSA. Associations between NAF PSA and paired serum hormone levels of the pituitary-ovarian axis were tested using the Spearman rank correlation and the Wilcoxon signed rank test. Analysis of variance on log transformed NAF PSA was used to determine the intra- and intervolunteer variability. Results: NAF PSA ranged from <0.003 to 133,330 ng PSA/g total protein (median 2,030 ng/g). No repeatable pattern of change was observed for individual volunteers and no significant association between NAF PSA and any pituitary-ovarian axis serum hormone was detected. There was no correlation between serum and NAF PSA. Conclusions: Weekly NAF sampling in healthy premenopausal women to provide adequate volumes for PSA analysis was successfully achieved. Considerable variation was observed for NAF PSA, which may limit the future potential for this tumor marker in NAF. This variability was not associated with hormones of the pituitary-ovarian axis and did not show repeated cyclical variability during the menstrual cycle. Serum PSA does not appear to be an acceptable indicator of NAF PSA levels.
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cellular characteristics of Nipple Aspiration fluid during the menstrual cycle in healthy premenopausal women
Cytopathology, 2001Co-Authors: Gillian Mitchell, P A Trott, L Morris, N Coleman, Edward R Sauter, Rosalind A EelesAbstract:Cellular characteristics of Nipple Aspiration fluid during the menstrual cycle in healthy premenopausal women. Fifteen healthy premenopausal female volunteers underwent weekly Nipple Aspiration of ductal fluid from both breasts during two menstrual cycles to investigate the variability of the cellular profile of the ductal fluid. Ductal fluid was successfully obtained using breast massage and Nipple-areolar suction from 247/280 (89%) breasts. 83% of samples available for cytological analysis were cellular and 30% of cellular aspirates contained ductal epithelial cells identified using standard morphological criteria. No significant variation in cell number or cell type was identified during the menstrual cycle. All samples tested had an 'H' score of zero for oestrogen receptor. Seven out of 14 women expressed the proliferation marker Mcm-2 in the cells of at least one of the specimens, with no evidence of a menstrual cycle influence on expression. In conclusion, the cellular profile of breast ductal fluid did not vary consistently during the menstrual cycle, permitting future breast cancer screening studies incorporating serial Nipple Aspirations to be performed independent of the phase of the cycle.