The Experts below are selected from a list of 45 Experts worldwide ranked by ideXlab platform

Wang Wei-lin - One of the best experts on this subject based on the ideXlab platform.

  • Analysis of NOS1,lipid and Nissl Body in the spina bifida occulta spinal cord
    Shandong Medical Journal, 2010
    Co-Authors: Wang Wei-lin
    Abstract:

    Objective To detect the amount of neuronal nitric oxide synthase 1(NOS1),lipid and Nissl Body in the spinal cord of spina bifida occulta(SBO) fetal rats and to approach its pathogenesy.Methods Rats in experimental group was established SBO fetal rat model.The expression of NOS1 was determined by immunofluorescence staining and the amount of lipid in spinal cord was determined by oil red O staining,while the amount of Nissl bodies in neurons was determined by toluidine blue staining.Results In comparison to the normal group,the NOS1 positive motoneurons were significantly increased in the malformation spinal cord.No significant difference of the amount of fat tissue in the spinal cord was observed between experimental group and normal group.The toluidine number decreased in the malformation specimens(P0.01).Conclusion It may be one of the important pathological bases of SBO that the amount of NOS1 in spinal cord increases,whereas Nissl Body decreases.

Jianjun Zhao - One of the best experts on this subject based on the ideXlab platform.

  • puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition
    Neuropharmacology, 2014
    Co-Authors: Nan Wang, Yanmin Zhang, Yuanji Wang, Yanjun Cao, Jianjun Zhao
    Abstract:

    Puerarin is extensively attractive because of its superior neuroprotective effects in stroke prevention. This paper focused on the protective effect of puerarin both in vivo and in vitro. Middle cerebral artery occlusion (MCAO) was operated on male Sprague-Dawley rat for 2 h, different doses of puerarin (2.62, 7.86 and 23.59 mg/kg) or vehicle were gavaged 1 h after reperfusion. Rats were sacrificed after 24 h or 7 days treatment of puerarin/vehicle. In 7.86 and 23.59 mg/kg groups, infarct volume was reduced (P < 0.05) when puerarin was given once; 7 days puerarin intervention further reduced the infarct volume (P < 0.05) compared with vehicle-treated animal. The modified neurological severity score (mNSS) was also raised in day 4 in 7.86 and 23.59 mg/kg groups and in all groups in day 7 compared with vehicle (P < 0.05). The number of Nissl Body, cleaved caspase-3 and GFAP positive cells increased observably after stroke in dose-dependence in rats. In our in vitro study, we have found that puerarin inhibited the pro-apoptosis factor and upregulated the BDNF secret of astrocytes after OGD-R. This indicated that the repairing effect of puerarin was associated with the astrocyte protection.

Weimin Ning - One of the best experts on this subject based on the ideXlab platform.

  • experimental evidence and network pharmacology identify the molecular targets of tong sheng tablets in cerebral ischemia reperfusion injury
    American Journal of Translational Research, 2019
    Co-Authors: Tuo Liu, Kangqiang Yang, Kang Zhou, Jing Tan, Jingyi Chen, Weimin Ning
    Abstract:

    Purpose Tong Sheng tablets (TSTs) have long been used for treating cerebral ischemic reperfusion injury (CIRI) in clinic, but the underlying mechanism remains unknown. Therefore, in this study, TSTs were evaluated systematically using chemical analysis, network pharmacology and classical pharmacology. Methods The first part was TSTs quality control including TSTs fingerprint establishment and chemicals identification. In the second part, network pharmacology analysis and bioinformatics were combined to construct a compound-target-disease network, which can screen out key targets or pathways, revealing complex molecule mechanism of TSTs. The last part was experiment verification. Classical pharmacology of TSTs was investigated in vivo to verify the results of network pharmacology. Results (1) Fingerprints of TSTs were established, and 11 characteristic peaks were identified using HPLC. (2) Network pharmacology and bioinformatics suggested that the protection of TSTs in treating CIRI might be related to regulation of oxidative stress, inflammation and apoptosis, and some key molecules such as Nrf2, IL-1β, TNF, Bcl-2 and Cyt-C involved in the pathways. (3) TSTs significantly improved neurologic behavior scores, decreased the areas of ischemic necrosis and neuronal necrosis, and increased Nissl Body counts. Besides, TSTs significantly decreased pro-inflammatory cytokine (IL-1β, TNF-α) and pro-oxidative product levels (LPO, MDA) and increased anti-oxidative product levels (NO, SOD). TSTs downregulated the protein expressions of Nrf2 and HO-1. Meanwhile, TSTs reduced apoptotic cell counts, downregulated the protein expressions of Cyt-C and Bax, and upregulated the protein expression of Bcl-2. In terms of autophagy, TSTs enhanced LC-3B protein expression. Conclusion The present results illustrated that TSTs effectively alleviated CIRI, and the underlying mechanism might be associated with multiple molecular pathways. Herein, we established a primary pattern for studying Chinese herbal compounds and provided basic guidance for future investigation.

Nan Wang - One of the best experts on this subject based on the ideXlab platform.

  • puerarin protected the brain from cerebral ischemia injury via astrocyte apoptosis inhibition
    Neuropharmacology, 2014
    Co-Authors: Nan Wang, Yanmin Zhang, Yuanji Wang, Yanjun Cao, Jianjun Zhao
    Abstract:

    Puerarin is extensively attractive because of its superior neuroprotective effects in stroke prevention. This paper focused on the protective effect of puerarin both in vivo and in vitro. Middle cerebral artery occlusion (MCAO) was operated on male Sprague-Dawley rat for 2 h, different doses of puerarin (2.62, 7.86 and 23.59 mg/kg) or vehicle were gavaged 1 h after reperfusion. Rats were sacrificed after 24 h or 7 days treatment of puerarin/vehicle. In 7.86 and 23.59 mg/kg groups, infarct volume was reduced (P < 0.05) when puerarin was given once; 7 days puerarin intervention further reduced the infarct volume (P < 0.05) compared with vehicle-treated animal. The modified neurological severity score (mNSS) was also raised in day 4 in 7.86 and 23.59 mg/kg groups and in all groups in day 7 compared with vehicle (P < 0.05). The number of Nissl Body, cleaved caspase-3 and GFAP positive cells increased observably after stroke in dose-dependence in rats. In our in vitro study, we have found that puerarin inhibited the pro-apoptosis factor and upregulated the BDNF secret of astrocytes after OGD-R. This indicated that the repairing effect of puerarin was associated with the astrocyte protection.

Tuo Liu - One of the best experts on this subject based on the ideXlab platform.

  • experimental evidence and network pharmacology identify the molecular targets of tong sheng tablets in cerebral ischemia reperfusion injury
    American Journal of Translational Research, 2019
    Co-Authors: Tuo Liu, Kangqiang Yang, Kang Zhou, Jing Tan, Jingyi Chen, Weimin Ning
    Abstract:

    Purpose Tong Sheng tablets (TSTs) have long been used for treating cerebral ischemic reperfusion injury (CIRI) in clinic, but the underlying mechanism remains unknown. Therefore, in this study, TSTs were evaluated systematically using chemical analysis, network pharmacology and classical pharmacology. Methods The first part was TSTs quality control including TSTs fingerprint establishment and chemicals identification. In the second part, network pharmacology analysis and bioinformatics were combined to construct a compound-target-disease network, which can screen out key targets or pathways, revealing complex molecule mechanism of TSTs. The last part was experiment verification. Classical pharmacology of TSTs was investigated in vivo to verify the results of network pharmacology. Results (1) Fingerprints of TSTs were established, and 11 characteristic peaks were identified using HPLC. (2) Network pharmacology and bioinformatics suggested that the protection of TSTs in treating CIRI might be related to regulation of oxidative stress, inflammation and apoptosis, and some key molecules such as Nrf2, IL-1β, TNF, Bcl-2 and Cyt-C involved in the pathways. (3) TSTs significantly improved neurologic behavior scores, decreased the areas of ischemic necrosis and neuronal necrosis, and increased Nissl Body counts. Besides, TSTs significantly decreased pro-inflammatory cytokine (IL-1β, TNF-α) and pro-oxidative product levels (LPO, MDA) and increased anti-oxidative product levels (NO, SOD). TSTs downregulated the protein expressions of Nrf2 and HO-1. Meanwhile, TSTs reduced apoptotic cell counts, downregulated the protein expressions of Cyt-C and Bax, and upregulated the protein expression of Bcl-2. In terms of autophagy, TSTs enhanced LC-3B protein expression. Conclusion The present results illustrated that TSTs effectively alleviated CIRI, and the underlying mechanism might be associated with multiple molecular pathways. Herein, we established a primary pattern for studying Chinese herbal compounds and provided basic guidance for future investigation.