The Experts below are selected from a list of 225 Experts worldwide ranked by ideXlab platform
Bruce A. Freeman - One of the best experts on this subject based on the ideXlab platform.
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several ...
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several of these species react with unsaturated fatty acid to yield nitrated oxidatIon products. Using HPLC coupled with both UV detectIon and electrospray IonizatIon mass spectrometry, products of reactIon of ONOO- with linoleic acid displayed mass/charge (m/z) characteristics of LNO2 (at least three products at m/z 324, negative Ion mode). Further analysis by MS/MS gave a major fragment at m/z 46. AdditIon of a NO2 group was confirmed using [15N]ONOO- which gave a product at m/z 325, fragmenting to form a daughter Ion at m/z 47. FormatIon of nitrated lipids was inhibited by bicarbonate, superoxide dismutase (SOD), and Fe3+−EDTA, while the yield of oxidatIon products was decreased by bicarbonate and SOD, but not by Fe3+−EDTA. ReactIon of linoleic acid with both nitrogen dioxide (•NO2) or Nitronium tetrafluoroborate (NO...
Jason P. Eiserich - One of the best experts on this subject based on the ideXlab platform.
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several ...
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several of these species react with unsaturated fatty acid to yield nitrated oxidatIon products. Using HPLC coupled with both UV detectIon and electrospray IonizatIon mass spectrometry, products of reactIon of ONOO- with linoleic acid displayed mass/charge (m/z) characteristics of LNO2 (at least three products at m/z 324, negative Ion mode). Further analysis by MS/MS gave a major fragment at m/z 46. AdditIon of a NO2 group was confirmed using [15N]ONOO- which gave a product at m/z 325, fragmenting to form a daughter Ion at m/z 47. FormatIon of nitrated lipids was inhibited by bicarbonate, superoxide dismutase (SOD), and Fe3+−EDTA, while the yield of oxidatIon products was decreased by bicarbonate and SOD, but not by Fe3+−EDTA. ReactIon of linoleic acid with both nitrogen dioxide (•NO2) or Nitronium tetrafluoroborate (NO...
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tyrosine modificatIon by reactive nitrogen species a closer look
1995Co-Authors: A Vandervliet, Jason P. Eiserich, C A Oneill, Barry Halliwell, Carroll E CrossAbstract:Peroxynitrite (ONOO-) is a powerful oxidant and cytotoxic species formed by the rapid reactIon between nitrogen monoxide (nitric oxide, .NO) and superoxide (O2.-). At neutral pH ONOO- is partly protonated and this protonated form, peroxynitrous acid (ONOOH), decomposes rapidly to nitrate, forming (an) intermediate(s) with reactivity similar to .OH and .NO2. Peroxynitrite can hydroxylate and nitrate aromatic rings, and aromatic nitratIon of phenols such as tyrosine by ONOOH is proposed to proceed via a radical mechanism, with intermediate formatIon of .NO2. ModificatIon of tyrosine by .NO2 also involves nitratIon via a radical mechanism. Aromatic nitratIon of phenols by ONOO- has been shown to be enhanced by superoxide dismutase or Fe(3+)-EDTA, which were proposed to catalyze heterolytic cleavage of ONOOH to form a nitrating species similar to the Nitronium Ion (NO2+). We investigated possible mechanisms of tyrosine modificatIon by various reactive nitrogen species, including ONOO-, 3-morpholinosydnonimine (SIN-1), and .NO2. ReactIon of tyrosine with ONOO- leads to formatIon of 3-nitrotyrosine and dityrosine, indicating intermediate formatIon of tyrosyl radicals. The pH dependence of formatIon of both 3-nitrotyrosine and dityrosine by ONOO- suggests that intermediate formatIon of ONOOH is required. Qualitatively similar results were obtained when ONOOH was generated continuously by H2O2 and NaNO2 at mildly acidic pH or with SIN-1, a compound which at neutral pH releases both .NO and O2.-, presumably producing ONOO-. However, relatively low yields of nitrotyrosine were obtained with SIN-1, possibly because of competing reactIons of tyrosyl radicals with .NO or O2.-. Possible involvement of .NO2 in tyrosine modificatIon by ONOO- was studied using hydroxyl radical scavengers, which can increase the radical yield during decompositIon of ONOOH and thereby enhance generatIon of .NO2. Hydroxyl radical scavengers did not affect tyrosine modificatIon by .NO2 directly and slightly inhibited tyrosine modificatIon by authentic ONOO-. However, when ONOO- was produced at a slower rate, either by SIN-1 or by H2O2/NaNO2 at acidic pH, hydroxyl radical scavengers were found to significantly enhance tyrosine nitratIon. Our results suggest that ONOO- or ONOO(-)-generating systems induce nitratIon of tyrosine (or tyrosine residues in proteins) via intermediate formatIon of tyrosyl radicals and .NO2.
Valerie B Odonnell - One of the best experts on this subject based on the ideXlab platform.
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several ...
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several of these species react with unsaturated fatty acid to yield nitrated oxidatIon products. Using HPLC coupled with both UV detectIon and electrospray IonizatIon mass spectrometry, products of reactIon of ONOO- with linoleic acid displayed mass/charge (m/z) characteristics of LNO2 (at least three products at m/z 324, negative Ion mode). Further analysis by MS/MS gave a major fragment at m/z 46. AdditIon of a NO2 group was confirmed using [15N]ONOO- which gave a product at m/z 325, fragmenting to form a daughter Ion at m/z 47. FormatIon of nitrated lipids was inhibited by bicarbonate, superoxide dismutase (SOD), and Fe3+−EDTA, while the yield of oxidatIon products was decreased by bicarbonate and SOD, but not by Fe3+−EDTA. ReactIon of linoleic acid with both nitrogen dioxide (•NO2) or Nitronium tetrafluoroborate (NO...
Michael J Jablonsky - One of the best experts on this subject based on the ideXlab platform.
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several ...
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several of these species react with unsaturated fatty acid to yield nitrated oxidatIon products. Using HPLC coupled with both UV detectIon and electrospray IonizatIon mass spectrometry, products of reactIon of ONOO- with linoleic acid displayed mass/charge (m/z) characteristics of LNO2 (at least three products at m/z 324, negative Ion mode). Further analysis by MS/MS gave a major fragment at m/z 46. AdditIon of a NO2 group was confirmed using [15N]ONOO- which gave a product at m/z 325, fragmenting to form a daughter Ion at m/z 47. FormatIon of nitrated lipids was inhibited by bicarbonate, superoxide dismutase (SOD), and Fe3+−EDTA, while the yield of oxidatIon products was decreased by bicarbonate and SOD, but not by Fe3+−EDTA. ReactIon of linoleic acid with both nitrogen dioxide (•NO2) or Nitronium tetrafluoroborate (NO...
Phillip Chumley - One of the best experts on this subject based on the ideXlab platform.
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several ...
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nitratIon of unsaturated fatty acids by nitric oxide derived reactive nitrogen species peroxynitrite nitrous acid nitrogen dioxide and Nitronium Ion
1999Co-Authors: Valerie B Odonnell, Jason P. Eiserich, Phillip Chumley, Michael J Jablonsky, Rama N Krishna, Marion Kirk, Stephen Barnes, Bruce A. FreemanAbstract:Reactive nitrogen species derived from nitric oxide are potent oxidants formed during inflammatIon that can oxidize membrane and lipoprotein lipids in vivo. Herein, it is demonstrated that several of these species react with unsaturated fatty acid to yield nitrated oxidatIon products. Using HPLC coupled with both UV detectIon and electrospray IonizatIon mass spectrometry, products of reactIon of ONOO- with linoleic acid displayed mass/charge (m/z) characteristics of LNO2 (at least three products at m/z 324, negative Ion mode). Further analysis by MS/MS gave a major fragment at m/z 46. AdditIon of a NO2 group was confirmed using [15N]ONOO- which gave a product at m/z 325, fragmenting to form a daughter Ion at m/z 47. FormatIon of nitrated lipids was inhibited by bicarbonate, superoxide dismutase (SOD), and Fe3+−EDTA, while the yield of oxidatIon products was decreased by bicarbonate and SOD, but not by Fe3+−EDTA. ReactIon of linoleic acid with both nitrogen dioxide (•NO2) or Nitronium tetrafluoroborate (NO...