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Parit Plainkum - One of the best experts on this subject based on the ideXlab platform.

  • efficacy of quantitative capillary beta hydroxybutyrate measurement in the diagnosis of diabetic ketoacidosis a comparison to quantitative serum ketone measurement by Nitroprusside Reaction
    Journal of the Medical Association of Thailand Chotmaihet thangphaet, 2014
    Co-Authors: Raweewan Lertwattanarak, Parit Plainkum
    Abstract:

    Objective: To examine the efficacy of using capillary beta-hydroxy butyrate (β-OHB) levels in comparison with serum ketone levels in distinguishing diabetic ketoacidosis (DKA) from non-DKA states in patients who had severe hyperglycemia and to determine a cut-off level of capillary β-OHB that is best for the diagnosis of DKA. Material and Method: Diabetic patients who presented with capillary blood glucose of >400 mg/dL were studied. Capillary β-OHB levels were measured by using a ketometer (OptiumXceedTM) at the same time as blood sample collection for biochemical tests and serum ketone measurement using Nitroprusside Reaction. The American Diabetes Association (ADA) criteria 2012 were used as the gold standard in the diagnosed of DKA. Results: There were 13 cases (34.2%) with DKA (DKA group) and 25 cases (65.8%) without DKA (non-DKA group). There was no difference in plasma glucose levels between both groups. (DKA group = 714.2+367.6 mg/dl vs. non-DKA group = 589.4+220.2 mg/dl). The DKA group had significantly higher serum ketone (7.2+3.6 vs. 0.28+0.05 mmol/L, p 3.1 mmol/L was the best cut-off level for the diagnosis of DKA, and yielded a sensitivity of 100% (95% CI = 75.1-100) with a specificity of 96% (95% CI = 79.6-99.3). Conclusion: Using a cut-off capillary β-OHB level of >3.1 mmol/L is highly effective in the diagnosis of DKA in patients who presented with hyperglycemia. Quantitative measurement of capillary β-OHB levels using a ketometer offers an immediate result that is useful for a reliable triage of screening for DKA in patients presented with severe hyperglycemia. Keywords: Triage, Diabetic ketoacidosis, Ketone, Beta-hydroxybutyrate

Raweewan Lertwattanarak - One of the best experts on this subject based on the ideXlab platform.

  • efficacy of quantitative capillary beta hydroxybutyrate measurement in the diagnosis of diabetic ketoacidosis a comparison to quantitative serum ketone measurement by Nitroprusside Reaction
    Journal of the Medical Association of Thailand Chotmaihet thangphaet, 2014
    Co-Authors: Raweewan Lertwattanarak, Parit Plainkum
    Abstract:

    Objective: To examine the efficacy of using capillary beta-hydroxy butyrate (β-OHB) levels in comparison with serum ketone levels in distinguishing diabetic ketoacidosis (DKA) from non-DKA states in patients who had severe hyperglycemia and to determine a cut-off level of capillary β-OHB that is best for the diagnosis of DKA. Material and Method: Diabetic patients who presented with capillary blood glucose of >400 mg/dL were studied. Capillary β-OHB levels were measured by using a ketometer (OptiumXceedTM) at the same time as blood sample collection for biochemical tests and serum ketone measurement using Nitroprusside Reaction. The American Diabetes Association (ADA) criteria 2012 were used as the gold standard in the diagnosed of DKA. Results: There were 13 cases (34.2%) with DKA (DKA group) and 25 cases (65.8%) without DKA (non-DKA group). There was no difference in plasma glucose levels between both groups. (DKA group = 714.2+367.6 mg/dl vs. non-DKA group = 589.4+220.2 mg/dl). The DKA group had significantly higher serum ketone (7.2+3.6 vs. 0.28+0.05 mmol/L, p 3.1 mmol/L was the best cut-off level for the diagnosis of DKA, and yielded a sensitivity of 100% (95% CI = 75.1-100) with a specificity of 96% (95% CI = 79.6-99.3). Conclusion: Using a cut-off capillary β-OHB level of >3.1 mmol/L is highly effective in the diagnosis of DKA in patients who presented with hyperglycemia. Quantitative measurement of capillary β-OHB levels using a ketometer offers an immediate result that is useful for a reliable triage of screening for DKA in patients presented with severe hyperglycemia. Keywords: Triage, Diabetic ketoacidosis, Ketone, Beta-hydroxybutyrate

Routh Clinkat Chemis+ry - One of the best experts on this subject based on the ideXlab platform.

  • A micro method for serum cholinesterase
    2015
    Co-Authors: Phil J. Garry, Joseph Routh I. J, Routh Clinkat Chemis+ry
    Abstract:

    A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetyithiocholine to release thiocholine, which reacts with 5:5-dithiobis- (2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 mm. at 370; the Reaction is stopped by the excess addition of quinidine sulfate. The difference in absorbance between the blank and test sample is then measured at 412 mL. Duplicate determinations can be run on 0.1 ml. of serum or plasma, and the method is well suited to laboratories using routine procedures. S EVERAL METHODS have been developed for the determination of cholin-esterase in a variety of biological specimens. These methods involve measurements of change in pH (1, 2); Reaction with hydroxylamine (3, 4); the use of acetyithiocholine as a substrate, followed by iodometric titration (5, 6); Reaction with Nitroprusside (7); decrease in absorbance of the thioester bond (8) ; and the Reaction of Sil groups with 5:5-dithio-bis-(2-nitrobenzoic acid) (9). Such an array of analytical procedures, coupled with complex instrumentation, incubation periods of up to 1 hr., and variation in expression of enzyme units have led to considerable confusion in clinical chemistry laboratories. It has already been established that acetylthiocholine is a satisfactory substrate for the measurement of cholinesterase activity (10). The SH groups of the thiocholine resulting from the enzyme action may be deter-mined colorimetrically by the Nitroprusside Reaction (7) or by Reaction with 5:5-dithiobis-(2-nitrobenzoic acid) (DTNB) originally proposed by Eliman in 1959 (11). A comparison of the use of these two reagents in our laboratory resulted in a choice of DTNB. Ellman and coworkers have utilized this reagent in a method for the determination of cholin-esterase in whole blood, erythrocytes, and tissue homogenates (9). A simple, rapid, colorimetric micro method for plasma or serum with acetyithiocholine as a substrate and DTNB as the chromogenic agent hat

Phil J. Garry - One of the best experts on this subject based on the ideXlab platform.

  • A micro method for serum cholinesterase
    2015
    Co-Authors: Phil J. Garry, Joseph Routh I. J, Routh Clinkat Chemis+ry
    Abstract:

    A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetyithiocholine to release thiocholine, which reacts with 5:5-dithiobis- (2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 mm. at 370; the Reaction is stopped by the excess addition of quinidine sulfate. The difference in absorbance between the blank and test sample is then measured at 412 mL. Duplicate determinations can be run on 0.1 ml. of serum or plasma, and the method is well suited to laboratories using routine procedures. S EVERAL METHODS have been developed for the determination of cholin-esterase in a variety of biological specimens. These methods involve measurements of change in pH (1, 2); Reaction with hydroxylamine (3, 4); the use of acetyithiocholine as a substrate, followed by iodometric titration (5, 6); Reaction with Nitroprusside (7); decrease in absorbance of the thioester bond (8) ; and the Reaction of Sil groups with 5:5-dithio-bis-(2-nitrobenzoic acid) (9). Such an array of analytical procedures, coupled with complex instrumentation, incubation periods of up to 1 hr., and variation in expression of enzyme units have led to considerable confusion in clinical chemistry laboratories. It has already been established that acetylthiocholine is a satisfactory substrate for the measurement of cholinesterase activity (10). The SH groups of the thiocholine resulting from the enzyme action may be deter-mined colorimetrically by the Nitroprusside Reaction (7) or by Reaction with 5:5-dithiobis-(2-nitrobenzoic acid) (DTNB) originally proposed by Eliman in 1959 (11). A comparison of the use of these two reagents in our laboratory resulted in a choice of DTNB. Ellman and coworkers have utilized this reagent in a method for the determination of cholin-esterase in whole blood, erythrocytes, and tissue homogenates (9). A simple, rapid, colorimetric micro method for plasma or serum with acetyithiocholine as a substrate and DTNB as the chromogenic agent hat

Joseph Routh I. J - One of the best experts on this subject based on the ideXlab platform.

  • A micro method for serum cholinesterase
    2015
    Co-Authors: Phil J. Garry, Joseph Routh I. J, Routh Clinkat Chemis+ry
    Abstract:

    A simple colorimetric method for determining serum or plasma cholinesterase has been described. The cholinesterase acts on acetyithiocholine to release thiocholine, which reacts with 5:5-dithiobis- (2-nitrobenzoic acid) to produce a yellow color. The activity is measured after incubation for 3 mm. at 370; the Reaction is stopped by the excess addition of quinidine sulfate. The difference in absorbance between the blank and test sample is then measured at 412 mL. Duplicate determinations can be run on 0.1 ml. of serum or plasma, and the method is well suited to laboratories using routine procedures. S EVERAL METHODS have been developed for the determination of cholin-esterase in a variety of biological specimens. These methods involve measurements of change in pH (1, 2); Reaction with hydroxylamine (3, 4); the use of acetyithiocholine as a substrate, followed by iodometric titration (5, 6); Reaction with Nitroprusside (7); decrease in absorbance of the thioester bond (8) ; and the Reaction of Sil groups with 5:5-dithio-bis-(2-nitrobenzoic acid) (9). Such an array of analytical procedures, coupled with complex instrumentation, incubation periods of up to 1 hr., and variation in expression of enzyme units have led to considerable confusion in clinical chemistry laboratories. It has already been established that acetylthiocholine is a satisfactory substrate for the measurement of cholinesterase activity (10). The SH groups of the thiocholine resulting from the enzyme action may be deter-mined colorimetrically by the Nitroprusside Reaction (7) or by Reaction with 5:5-dithiobis-(2-nitrobenzoic acid) (DTNB) originally proposed by Eliman in 1959 (11). A comparison of the use of these two reagents in our laboratory resulted in a choice of DTNB. Ellman and coworkers have utilized this reagent in a method for the determination of cholin-esterase in whole blood, erythrocytes, and tissue homogenates (9). A simple, rapid, colorimetric micro method for plasma or serum with acetyithiocholine as a substrate and DTNB as the chromogenic agent hat