The Experts below are selected from a list of 66 Experts worldwide ranked by ideXlab platform
Werner Liesack - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of gene sequences encoding ammonia monooxygenase of Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71
FEMS microbiology letters, 1995Co-Authors: Jan-henrich Rotthauwe, Wietse De Boer, Werner LiesackAbstract:DNA encoding ammonia monooxygenase from two phylogenetically related autotrophic nitrifying bacteria, Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71, was amplified by PCR. The resulting products were cloned into the vector pCR-Script. A continuous region of DNA of about 1.5 kb for strain AHB1 and 1.24 kb for N. multiformis C-71 was analysed. These comprised the major part of the gene amoA encoding the active site polypeptide and, directly downstream, the 5′ portion of the amoB gene. The identity values for these sequences at the amino acid level were 93.0% for amoA and 96.1% for amoB. The corresponding values for the nucleic acid sequences were 86.7% and 88.8%, respectively. The identity of the 16S rRNA gene of strain AHB1 to that of N. multiformis C-71 was at least 98.5%. The different degree of sequence conservation between the 16S rDNA and the genes encoding for ammonia monooxygenase facilitates the application of the latter as a molecular tool for a fine-scale differentiation of autotrophic nitrifying bacteria, at the species or strain level, in both environmental and cultivation studies.
Jan-henrich Rotthauwe - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of gene sequences encoding ammonia monooxygenase of Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71
FEMS microbiology letters, 1995Co-Authors: Jan-henrich Rotthauwe, Wietse De Boer, Werner LiesackAbstract:DNA encoding ammonia monooxygenase from two phylogenetically related autotrophic nitrifying bacteria, Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71, was amplified by PCR. The resulting products were cloned into the vector pCR-Script. A continuous region of DNA of about 1.5 kb for strain AHB1 and 1.24 kb for N. multiformis C-71 was analysed. These comprised the major part of the gene amoA encoding the active site polypeptide and, directly downstream, the 5′ portion of the amoB gene. The identity values for these sequences at the amino acid level were 93.0% for amoA and 96.1% for amoB. The corresponding values for the nucleic acid sequences were 86.7% and 88.8%, respectively. The identity of the 16S rRNA gene of strain AHB1 to that of N. multiformis C-71 was at least 98.5%. The different degree of sequence conservation between the 16S rDNA and the genes encoding for ammonia monooxygenase facilitates the application of the latter as a molecular tool for a fine-scale differentiation of autotrophic nitrifying bacteria, at the species or strain level, in both environmental and cultivation studies.
E. Bock - One of the best experts on this subject based on the ideXlab platform.
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Genera-Specific Immunofluorescence Labeling of Ammonia Oxidizers with Polyclonal Antibodies Recognizing Both Subunits of the Ammonia Monooxygenase
Microbial Ecology, 2004Co-Authors: C. Fiencke, E. BockAbstract:Polyclonal antibodies that recognize the two subunits AmoA and AmoB of the ammonia monooxygenase (AMO) were applied to identify ammonia-oxidizing bacteria by immunofluorescence (IF) labeling in pure, mixed, and enriched cultures. The antibodies against the AmoA were produced using a synthetic peptide of the AmoA of Nitrosomonas eutropha , whereas the antibodies against the AmoB had been developed previously is against the whole B-subunit of the AMO [Pinck et al. (2001) Appl Environ Microbiol 67:118–124]. Using IF labeling, the AmoA antibodies were specific for the detection of all species of the genus Nitrosomonas . In contrast, the antiserum against AmoB labeled all genera of ammonia oxidizers of the β-subclass of Proteobacteria ( Nitrosomonas , Nitrosospira , Nitrosolobus , and Nitrosovibrio ). The fluorescence signals of the AmoA antibodies were spread all over the cells, whereas the signals of the AmoB antibodies were associated with the cytoplasmic membranes. The specificity of the reactions of the antisera with ammonia oxidizers were proven in pure and mixed cultures, and the characteristic IF labeling and the morphology of the cells enabled their identification at the genus level. The genus-specific IF labeling could be used to identify ammonia oxidizers enriched from various habitats. In enrichment cultures of natural sandstone, cells of the genera Nitrosomonas , Nitrosovibrio , and Nitrosospira were detected. Members of the genus Nitrosovibrio and Nitrosolobus were most prominent in enriched garden soil samples, whereas members of the genus Nitrosomonas dominated in enriched activated sludge. The antibodies caused only slight background fluorescence on sandstone and soil particles compared to oligonucleotide probes, which could not be used to detect ammonia oxidizers on these materials because of strong nonspecific fluorescence.
Wietse De Boer - One of the best experts on this subject based on the ideXlab platform.
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Comparative analysis of gene sequences encoding ammonia monooxygenase of Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71
FEMS microbiology letters, 1995Co-Authors: Jan-henrich Rotthauwe, Wietse De Boer, Werner LiesackAbstract:DNA encoding ammonia monooxygenase from two phylogenetically related autotrophic nitrifying bacteria, Nitrosospira sp. AHB1 and Nitrosolobus multiformis C-71, was amplified by PCR. The resulting products were cloned into the vector pCR-Script. A continuous region of DNA of about 1.5 kb for strain AHB1 and 1.24 kb for N. multiformis C-71 was analysed. These comprised the major part of the gene amoA encoding the active site polypeptide and, directly downstream, the 5′ portion of the amoB gene. The identity values for these sequences at the amino acid level were 93.0% for amoA and 96.1% for amoB. The corresponding values for the nucleic acid sequences were 86.7% and 88.8%, respectively. The identity of the 16S rRNA gene of strain AHB1 to that of N. multiformis C-71 was at least 98.5%. The different degree of sequence conservation between the 16S rDNA and the genes encoding for ammonia monooxygenase facilitates the application of the latter as a molecular tool for a fine-scale differentiation of autotrophic nitrifying bacteria, at the species or strain level, in both environmental and cultivation studies.
D W Hopkins - One of the best experts on this subject based on the ideXlab platform.
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different sensitivities to oxygen of nitrous oxide production by nitrosomonas europaea and Nitrosolobus multiformis
Soil Biology & Biochemistry, 2001Co-Authors: L Dundee, D W HopkinsAbstract:Abstract We measured N 2 O production by two species of primary chemoautotrophic nitrifying bacteria, Nitrosomonas europaea and Nitrosolobus multiformis , in media with different dissolved O 2 concentrations. Both species produced N 2 O across a wide range of O 2 concentrations and after 48 h there was a large peak of N 2 O production (3.2 nmol N 2 O cm −3 cell suspension) by N. europaea at a dissolved concentration of 2.1 mg O 2 l −1 , whereas for N. multiformis the peak in N 2 O production was much smaller (1.6 nmol N 2 O cm −3 ), but it also occurred at a dissolved O 2 concentration of 2.1 mg O 2 l −1 . However at greater dissolved oxygen concentrations (between 24 and 37 mg O 2 l −1 ), N. multiformis produced more N 2 O than N. europaea . The results are consistent with both species producing N 2 O during NH 4 + oxidation and with N. europaea , producing a much larger amount of N 2 O during denitrification than N. multiformis .