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Suresh P Vyas - One of the best experts on this subject based on the ideXlab platform.
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non ionic Surfactant based vesicles niosomes for non invasive topical genetic immunization against hepatitis b
International Journal of Pharmaceutics, 2005Co-Authors: Suresh P Vyas, Sanyog Jain, Vivek Mishra, Sunil Mahor, Paramjit Singh, Prem N Gupta, Amit Rawat, R. P. Singh, Prakash K DubeyAbstract:DNA vaccines are capable of eliciting both humoral as well as cellular immune responses. Liposomes have been widely employed for DNA delivery through topical route; however, they suffer from certain drawbacks like higher cost and instability. In present study, Non-Ionic Surfactant based vesicles (niosomes) for topical DNA delivery have been developed. DNA encoding hepatitis B surface antigen (HBsAg) was encapsulated in niosomes. Niosomes composed of span 85 and cholesterol as constitutive lipids were prepared by reverse phase evaporation method. Prepared niosomes were characterized for their size, shape and entrapment efficiency. The immune stimulating activity was studied by measuring serum anti-HBsAg titer and cyokines level (IL-2 and IFN-γ) following topical application of niosomes in Balb/c mice and results were compared with naked DNA and liposomes encapsulated DNA applied topically as well as naked DNA and pure recombinant HBsAg administered intramuscularly. It was observed that topical niosomes elicited a comparable serum antibody titer and endogenous cytokines levels as compared to intramuscular recombinant HBsAg and topical liposomes. The study signifies the potential of niosomes as DNA vaccine carriers for effective topical immunization. The proposed system is simple, stable and cost effective compared to liposomes.
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non ionic Surfactant based vesicles niosomes in drug delivery
International Journal of Pharmaceutics, 1998Co-Authors: Ijeoma F Uchegbu, Suresh P VyasAbstract:Abstract The self assembly of Non-Ionic Surfactants into vesicles was first reported in the seventies by researchers in the cosmetic industry. Since then a number of groups world wide have studied Non-Ionic Surfactant vesicles (niosomes) with a view to evaluating their potential as drug carriers. This article presents a summary of the achievements in the field to date. Niosomes may be formed form a diverse array of amphiphiles bearing sugar, polyoxyethylene, polyglycerol, crown ether and amino acid hydrophilic head groups and these amphiphiles typically possess one to two hydrophobic alkyl, perfluoroalkyl or steroidal groups. The self assembly of Surfactants into niosomes is governed not only by the nature of the Surfactant but by the presence of membrane additives, the nature of the drug encapsulated and the actual method of preparation. Methods of niosome preparation and the number of different morphologies that have been identified are detailed. The influence of formulation factors on niosome stability is also examined as are methods to optimise drug loading. In vivo these systems have been evaluated as immunological adjuvants, anti-cancer/anti-infective drug targeting agents and carriers of anti-inflammatory drugs. Niosomes have also been used in diagnostic imaging. Efforts to achieve transdermal and ophthalmic drug delivery with some formulations are also discussed.
Maria Carafa - One of the best experts on this subject based on the ideXlab platform.
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novel ph sensitive non ionic Surfactant vesicles comparison between tween 21 and tween 20
Colloids and Surfaces B: Biointerfaces, 2011Co-Authors: Luisa Di Marzio, Carlotta Marianecci, Mariadea Petrone, Federica Rinaldi, Maria CarafaAbstract:Abstract Drug delivery systems using vesicular carriers such as liposomes or niosomes, have distinct advantages over conventional dosage forms because the vesicles can act as drug containing reservoirs and the modification of the vesicular compositions or surface properties can adjust the drug release rate and/or the affinity for the target site. In recent years, niosomes have been the object of growing scientific attention as an alternative potential drug delivery system to conventional liposomes. The aim of present work was firstly to determine the critical micelle concentration (CMC) and then to analyze the capability of polysorbate 21 (Tween 21) to form niosomal formulations. Non-Ionic Surfactant vesicles were prepared using Tween 21 and cholesterol (CHOL) at equimolar ratio (15 mM:15 mM) by employing the “film” technique. Cholesterol was used to complete the hydrophobic moiety of single alkyl chain Non-Ionic Surfactant for vesicle formation. Dynamic light scattering was used to determine the size, zeta (ζ)-potential, polydispersity index and colloidal stability of the niosomal formulation. The vesicles were also characterized for their microviscosity and pH-sensitivity using fluorescent probes. The present work led to a simple, but positive result in pharmaceutical technology area. In particular, we have shown that the Tween 21:CHOL vesicles (i) are a homogenous and monodisperse vesicular population; (ii) are characterized by dimension compatible with the transport of drugs across biological barriers especially those whose diameter is about 100 nm; (iii) shows a good stability at least 90 days at 4 °C and (iv) are pH-sensitive systems. In conclusion, this niosomal formulation could be used as pH-sensitive nanodevices for delivery of drugs to pathological tissues, which exhibit an acidic environment as compared to normal tissues.
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non ionic Surfactant vesicles in pulmonary glucocorticoid delivery characterization and interaction with human lung fibroblasts
Journal of Controlled Release, 2010Co-Authors: Carlotta Marianecci, Donatella Paolino, Christian Celia, Massimo Fresta, Maria Carafa, Franco AlhaiqueAbstract:Abstract Non-Ionic Surfactant vesicles (NSVs) were proposed for the pulmonary delivery of glucocorticoids such as beclomethasone dipropionate (BDP) for the treatment of inflammatory lung diseases, e.g. asthma, chronic obstructive pulmonary disease and various type of pulmonary fibrosis. The thin layer evaporation method followed by sonication was used to prepare small Non-Ionic Surfactant vesicles containing beclomethasone dipropionate. Light scattering experiments showed that beclomethasone dipropionate-loaded Non-Ionic Surfactant vesicles were larger than unloaded ones and showed a significant ( P in vitro investigation of vesicle tolerability, carrier-cell interaction, intracellular drug uptake and drug-loaded vesicle anti-inflammatory activity. The investigated NSVs did not show a significant cytotoxic activity at all incubation times for concentrations ranging from 0.01 to 1 μM. Confocal laser scanning microscopy showed vesicular carrier localization at the level of the cytoplasm compartment, where the glucocorticoid receptor (target site) is localized. BDP-loaded Non-Ionic Surfactant vesicles elicited a significant improvement of the HLF intracellular uptake of the drug with respect to the free drug solution, drug/Surfactant mixtures and empty vesicles used as references. The treatment of HLF cells with BDP-loaded Non-Ionic Surfactant vesicles determined a noticeable increase of the drug anti-inflammatory activity by reducing the secretion of both constitutive and interleukin-1β-stimulated nerve growth factor (as inflammatory index) of 68% and 85%, respectively. Obtained data indicate that the investigated NSVs represent a promising tool as a pulmonary drug delivery system.
Weien Yuan - One of the best experts on this subject based on the ideXlab platform.
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advances of non ionic Surfactant vesicles niosomes and their application in drug delivery
Pharmaceutics, 2019Co-Authors: Minyan Wei, Weien YuanAbstract:Non-Ionic Surfactant based vesicles, also known as niosomes, have attracted much attention in pharmaceutical fields due to their excellent behavior in encapsulating both hydrophilic and hydrophobic agents. In recent years, it has been discovered that these vesicles can improve the bioavailability of drugs, and may function as a new strategy for delivering several typical of therapeutic agents, such as chemical drugs, protein drugs and gene materials with low toxicity and desired targeting efficiency. Compared with liposomes, niosomes are much more stable during the formulation process and storage. The required pharmacokinetic properties can be achieved by optimizing components or by surface modification. This novel delivery system is also easy to prepare and scale up with low production costs. In this paper, we summarize the structure, components, formulation methods, quality control of niosome and its applications in chemical drugs, protein drugs and gene delivery.
Prakash K Dubey - One of the best experts on this subject based on the ideXlab platform.
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non ionic Surfactant based vesicles niosomes for non invasive topical genetic immunization against hepatitis b
International Journal of Pharmaceutics, 2005Co-Authors: Suresh P Vyas, Sanyog Jain, Vivek Mishra, Sunil Mahor, Paramjit Singh, Prem N Gupta, Amit Rawat, R. P. Singh, Prakash K DubeyAbstract:DNA vaccines are capable of eliciting both humoral as well as cellular immune responses. Liposomes have been widely employed for DNA delivery through topical route; however, they suffer from certain drawbacks like higher cost and instability. In present study, Non-Ionic Surfactant based vesicles (niosomes) for topical DNA delivery have been developed. DNA encoding hepatitis B surface antigen (HBsAg) was encapsulated in niosomes. Niosomes composed of span 85 and cholesterol as constitutive lipids were prepared by reverse phase evaporation method. Prepared niosomes were characterized for their size, shape and entrapment efficiency. The immune stimulating activity was studied by measuring serum anti-HBsAg titer and cyokines level (IL-2 and IFN-γ) following topical application of niosomes in Balb/c mice and results were compared with naked DNA and liposomes encapsulated DNA applied topically as well as naked DNA and pure recombinant HBsAg administered intramuscularly. It was observed that topical niosomes elicited a comparable serum antibody titer and endogenous cytokines levels as compared to intramuscular recombinant HBsAg and topical liposomes. The study signifies the potential of niosomes as DNA vaccine carriers for effective topical immunization. The proposed system is simple, stable and cost effective compared to liposomes.
B W Brooks - One of the best experts on this subject based on the ideXlab platform.
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phase behaviour of a non ionic Surfactant polymeric solution water system during the phase inversion process
Colloids and Surfaces A: Physicochemical and Engineering Aspects, 2005Co-Authors: F Xie, B W BrooksAbstract:Abstract A phase inversion method for making colloidal polymer composites is being considered. That method requires the formation of fine and stable emulsions of polymerisable monomer containing another kind of polymer. Suitable emulsions might be produced via phase inversion. Therefore, the phase behaviour of dispersions containing Non-Ionic Surfactant, non-aqueous polymer solutions (polyisobutene in styrene) and was investigated. Phase inversion maps, showing the behaviour of the emulsion system as a function of hydrophilic–lipophilic balance (HLB) and water volume fraction, are developed. Two types of Non-Ionic Surfactants, polyoxyethylene sorbitanmonolaurate (SML), or polyoxyethylene nonylphenyl ether (NPE), were used in the study. There are two types of phase inversion (catastrophic and transitional) and, in some circumstances, complex drops may be formed. Five phase inversion boundaries have been found in the NPE system. These include one transitional inversion boundary and four catastrophic inversion boundaries. Unlike the NPE system, there are two transitional phase inversion boundaries in the SML system. With the non-aqueous solutions used here, a stable polymer emulsion with sub-micron polymer drops could not be produced by using the transitional inversion route with either Surfactant. However, it was possible to produce a stable polymer emulsion with sub-micron drops by approaching the catastrophic inversion condition. A drop size of about 500 nm was obtained by using a mixture of SML Surfactants. As the “unstable” catastrophic inversion region is approached closely, the complex drops increased in size and became less stable.
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phase inversion in non ionic Surfactant oil water systems i the effect of transitional inversion on emulsion drop sizes
Chemical Engineering Science, 1994Co-Authors: B W Brooks, Howard N RichmondAbstract:Abstract A study has been made of the dynamics of transitional-phase inversion in agitated liquid—liquid dispersions of hydrocarbon (“oil”) and water stabilised by Non-Ionic Surfactants. Transitional-phase inversions are caused by changes in the phase behaviour of a Non-Ionic Surfactant—oil—water system and are induced by altering the Surfactant's affinity for the oil and water phases. Drop sizes were found to decrease and emulsification rates to increase as the transitional inversion point was approached. Agitation conditions have no effect on drop sizes of three-phase emulsions present at the transitional inversion point and spontaneous emulsification was observed. Drop sizes of emulsions produced by transitional inversion are dependent on Surfactant type, Surfactant concentration and the emulsion's start condition. A quantitative analysis of the drop sizes of Winsor type 3 emulsions showed that the drop sizes are controlled by the area of interfacial Surfactant coverage. The viscosity of the oil phase can affect drop sizes produced by transitional inversion. A comparison of transitional inversion and direct emulsification shows that much finer emulsions, with much less energy input, can be produced by transitional inversion processes.